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The RING zinc finger protein LbRZF1 promotes salt gland development and salt tolerance in Limonium bicolor

Journal of Integrative Plant Biology 2024年第4期66卷 787-809页

作者： Zongran Yang Ziwei Zhang Ziqi Qiao Xueying Guo yixuan wen Yingxue Zhou Chunliang Yao Hai Fan Baoshan Wang Guoliang Han Shandong Provincial Key Laboratory of Plant Stress College of Life SciencesShandong Normal UniversityJi'nan 250014China National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta of Shandong ProvinceDongying 257000China Dongying Institute Shandong Normal UniversityDongying 257000China

The recretohalophyte Limonium bicolor thrives in high-salinity environments because salt glands on the above-ground parts of the plant help to expel excess salt.Here,we characterize a nucleus-localized C3HC4(RING-HC)-type zinc finger protein of L.bicolor named RING ZINC FINGER PROTEIN 1(LbRZF1).LbRZF1 was expressed in salt glands and in response to NaCl treatment.LbRZF1 showed no E3 ubiquitin ligase activity.The phenotypes of overexpression and knockout lines for LbRZF1 indicated that LbRZF1 positively regulated salt gland development and salt tolerance in L.bicolor.lbrzf1 mutants had fewer salt glands and secreted less salt than did the wild-type,whereas LbRZF1-overexpressing lines had opposite phenotypes,in keeping with the overall salt tolerance of these plants.A yeast two-hybrid screen revealed that LbRZF1 interacted with LbCATALASE2(LbCAT2)and the transcription factor LbMYB113,leading to their stabilization.Silencing of LbCAT2 or LbMYB113 decreased salt gland density and salt tolerance.The heterologous expression of LbRZF1 in Arabidopsis thaliana conferred salt tolerance to this non-halophyte.We also identified the transcription factor LbMYB48 as an upstream regulator of LbRZF1 transcription.The study of LbRZF1 in the regulation network of salt gland development also provides a good foundation for transforming crops and improving their salt resistance.

关键词： LbRZF1 Limonium bicolor molecular mechanism salt gland development salt tolerance

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Simulation Method and Feature Analysis of Shutdown Pressure Evolution During Multi-Cluster Fracturing Stimulation

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Energy Engineering 2024年第1期121卷 111-123页

作者： Huaiyin He Longqing Zou Yanchao Li yixuan Wang Junxiang Li Huan wen Bei Chang Lijun Liu Shale Gas Exploration and Development Department CNPC Chuanqing Drilling Engineering Co.Ltd.Chengdu610051China College of Energy Chengdu University of TechnologyChengdu610059China

Multistage multi-cluster hydraulic fracturing has enabled the economic exploitation of shale reservoirs,but the interpretation of hydraulic fracture parameters is challenging.The pressure signals after pump shutdown are influenced by hydraulic fractures,which can reflect the geometric features of hydraulic fracture.The shutdown pressure can be used to interpret the hydraulic fracture parameters in a real-time and cost-effective manner.In this paper,a mathematical model for shutdown pressure evolution is developed considering the effects of wellbore friction,perforation friction and fluid loss in fractures.An efficient numerical simulation method is established by using the method of characteristics.Based on this method,the impacts of fracture half-length,fracture height,opened cluster and perforation number,and filtration coefficient on the evolution of shutdown pressure are analyzed.The results indicate that a larger fracture half-length may hasten the decay of shutdown pressure,while a larger fracture height can slow down the decay of shutdown pressure.A smaller number of opened clusters and perforations can significantly increase the perforation friction and decrease the overall level of shutdown pressure.A larger filtration coefficient may accelerate the fluid filtration in the fracture and hasten the drop of the shutdown pressure.The simulation method of shutdown pressure,as well as the analysis results,has important implications for the interpretation of hydraulic fracture parameters.

关键词： Multistage multi-cluster hydraulic fracturing pump shutdown pressure feature analysis numerical simulation

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Positive effects of biochar application and Rhizophagus irregularis inoculation on mycorrhizal colonization,rice seedlings and phosphorus cycling in paddy soils

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Pedosphere 2024年第2期34卷 361-373页

作者： yixuan CHEN Zhonghua wen Jun MENG Zunqi LIU Jialong WEI Xiyu LIU Ziyi GE Wanning DAI Li LIN wenfu CHEN National Biochar Institute Shenyang Agricultural UniversityShenyang 110866China Key Laboratory of Biochar and Soil Improvement Ministry of Agriculture and Rural AffairsShenyang 110866China

Phosphorus(P)is an essential element for plant growth but is often limiting in ecosystems;therefore,improving the P fertilizer use efficiency is important.Biochar and arbuscular mycorrhizal fungi(AMF)may enhance P cycling in paddy soils that contain high content of total P but low content of available P(AP).In this study,the effects of biochar addition and Rhizophagus irregularis inoculation on the organic and inorganic P contents and phosphatase activities in paddy soils,rice seedling growth,and AMF colonization were investigated.Compared with no biochar addition,biochar addition enhanced the percentage of spore germination at day 7,hyphal length,most probable number,and mycorrhizal colonization rate of R.irregularis by 32%,662%,70%,and 28%on average,respectively.Biochar and R.irregularis altered soil P cycling and availability.Biochar and R.irregularis,either individually or in combination,increased soil AP content by 2%–48%.Rice seedlings treated with biochar and R.irregularis produced greater biomass,improved root morphology,and increased nutrient uptake compared with those of the control without biochar and R.irregularis.The results suggest that combined application of biochar and R.irregularis is beneficial to rice cultivation in paddy soils with high content of total P but low content of AP.

关键词： arbuscular mycorrhizal fungi available P phosphorus fraction rice husk biochar seedling growth spore germination

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In situ TEM visualization of Ag catalysis in Li-O_(2)nanobatteries

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Nano Research 2023年第5期16卷 6833-6839页

作者： yixuan wen Shuaijun Ding Chongchong Ma Peng Jia Wei Tu Yunna Guo Shuang Guo Wei Zhou Xiaoqian Zhang Jianyu Huang Liqiang Zhang Tongde Shen Yuqing Qiao Hebei Key Laboratory of Applied Chemistry College of Environmental and Chemical EngineeringYanshan UniversityQinhuangdao 066004China Clean Nano Energy Center State Key Laboratory of Metastable Materials Science and TechnologyYanshan UniversityQinhuangdao 066004China

Lithium-oxygen(Li-O_(2))batteries have been considered as an ideal solution to solving the global energy crisis.Silver(Ag)and Agbased catalyst have been extensively studied due to their high catalytic activities in Li-O_(2)batteries.However,it remains a challenge to track the catalytic mechanism during the charge/discharge process.Here,a nanoscale processing method was used to assemble a Li-O_(2)nanobattery in an aberration-corrected environmental transmission electron microscope(ETEM),where a single Ag nanowire(NW)was used as catalyst for O_(2)electrode.A visualization of the lithium ion insertion process during the electrochemical reactions was achieved in this nanobattery.Numerous Ag nanoparticles(NPs)were observed on the surface of the Ag NW,which were covered by the discharge product Li2O_(2).By simultaneously studying the evolution of the interface and the phase transformation,it can be concluded that these Ag NPs wrapped around Ag NW acted as catalyst during the subsequent charge/discharge reaction.Based on these studies,Ag NPs decorated on porous carbon were synthesized,it can simultaneously improve the cycling stability(100 cycles)and the maximum specific capacity(17,371 mAh·g^(−1)at a current density of 100 mA·g^(−1))in a coin cell Li-O_(2)battery.This study suggests that nanoscale Ag may be a promising catalyst for Li-O_(2)battery.

关键词： Li-O_(2)battery in situ environmental transmission electron microscopy catalyst Ag nanoparticles

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Strategies for Video Producers to Tell Their Good Stories Under the 5W Model of Communication

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教育技术与创新 2024年第1期6卷 50-66页

作者： wenqian Hong yixuan Jiang Rui wen wenyu Chen Nan Lou Jinxuan Deng Jiayun Li Hanfei Li Dan Ye School of Foreign Studies Wenzhou UniversityChina

In recent years,with the rise of video media such as YouTube and Tik Tok,the short video creation industry has emerged.How to spread China's excellent culture and how to“tell a good Chinese story”have become a hot concern.Using the 5W Model of Communication as a framework for analysis,this paper selects Chinese short videos with high popularity on YouTube as the object of the study.Studies found that the success of these videos was associated with five factors in the process of communication,which are communication main forces,communication content,communication channels,communication audience,and audience effects.Through theoretical and data analysis,the study concluded five strategies in video production to help promote Intercultural communication.

关键词： International communication communication strategy video production

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章太炎进化观的历时性研究

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新疆社会科学 2024年第1期 21-33,164页

作者：张翊轩北京大学哲学系北京100871

1903—1906年间,章太炎的进化观发生了鲜明的“断裂”,在《訄书》时期,章太炎认同达尔文、斯宾塞的进化论;至《俱分进化论》时期,他提出了善恶苦乐并进的俱分进化观,并把“俱分”进一步推扩为“文明”的定语;到《民报》时期,特别是在《... 详细信息

1903—1906年间,章太炎的进化观发生了鲜明的“断裂”,在《訄书》时期,章太炎认同达尔文、斯宾塞的进化论;至《俱分进化论》时期,他提出了善恶苦乐并进的俱分进化观,并把“俱分”进一步推扩为“文明”的定语;到《民报》时期,特别是在《齐物论释》中,批判线性进化观背后的理论根源逐步呈现出来,表达为“齐文野”的思想旨趣及对目的论文明观的深刻反思,对进化概念本身的批判实则是对诸如文明、进步等观念被用以将强权和暴力合理化、合法化、合目的性的危险的格外警惕,这是理解章太炎一以贯之之道的方便法门。

关键词：章太炎断裂《俱分进化论》齐文野目的论

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RNA-Seq analysis reveals the growth and photosynthetic responses of rapeseed(Brassica napus L.)under red and blue LEDs with supplemental yellow,green,or white light

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Horticulture Research 2020年第1期7卷 296-308页

作者： Xiaoying Liu Zheng Chen Mohammad Shah Jahan yixuan wen Xuyang Yao Haifeng Ding Shirong Guo Zhigang Xu College of Agriculture Nanjing Agricultural University210095 NanjingChina College of Horticulture Nanjing Agricultural University210095 NanjingChina Department of Horticulture Sher-e-Bangla Agricultural UniversityDhaka 1207Bangladesh College of Agriculture Shihezi University832003 ShiheziChina

Compound light is required for plant growth and development,but the response mechanisms of plants are undercharacterized and not fully understood.The present study was undertaken to evaluate the effect of supplemental light(green light,G;white light,W;yellow light,Y)added to red–blue light(RB)and sole W on the growth and photosynthesis of rapeseed seedlings.The results revealed that supplemental G/W improved the growth and photosynthesis of seedlings,but supplemental Y significantly reduced the photosynthetic rate and palisade tissue layer.Sole W caused similar responses in terms of growth,leaf development,oxidative damage,and antioxidant capability as supplemental Y.In total,449,367,813,and 751 differentially expressed genes(DEGs)were identified under supplemental G,Y,and W and sole W,respectively,compared to RB.The DEGs under different lights were closely associated with pathways such as light stimulus and high-light response,root growth,leaf development,photosynthesis,photosynthesis-antenna proteins,carbohydrate synthesis and degradation,secondary metabolism,plant hormones,and antioxidant capacity,which contributed to the distinct growth and photosynthesis under different treatments.Our results suggest that Y is more likely substituted by other wavelengths to achieve certain effects similar to those of supplemental Y,while G has a more distinctive effect on rapeseed.Taken together,supplementation RB with G/W promotes the growth of rapeseed seedlings in a controlled environment.

关键词： substituted oxidative synthesis

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基于用户偏好的多媒体视频个性化智能推荐方法研究

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电子设计工程 2024年第11期32卷 192-195页

作者：董雯曹奕萱于小婷西北大学现代学院陕西西安710130

为了满足用户个性化需求,提出基于用户偏好的多媒体视频个性化智能推荐方法。采集用户历史浏览行为数据,对提取的视频特征向量作相似度计算,生成视频候选集,建立视频浏览兴趣点(PoI)关联图,根据用户浏览时间计算其对不同类别视频PoI的... 详细信息

为了满足用户个性化需求,提出基于用户偏好的多媒体视频个性化智能推荐方法。采集用户历史浏览行为数据,对提取的视频特征向量作相似度计算,生成视频候选集,建立视频浏览兴趣点(PoI)关联图,根据用户浏览时间计算其对不同类别视频PoI的兴趣偏好度,选择排名靠前的N个视频生成推荐列表,实现多媒体视频个性化智能推荐。实验结果表明,该方法可实现多媒体视频个性化推荐,当其维度为180时,视频推荐的Top-1指标最高;兴趣吻合度指标均值达到94.9%,Top-1均值为95.51%。

关键词：用户偏好多媒体视频个性化推荐词向量兴趣点(PoI) 推荐列表

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基于多种降水数据产品的黄河源区径流模拟研究

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高原气象 2024年第3期43卷 570-582页

作者：李晓玥文军谢琰陈亚玲陈怡璇葛翔宇成都信息工程大学大气科学学院/高原大气与环境四川省重点实验室四川成都610225 宜宾学院四川宜宾644000

黄河源区位于青藏高原的东北部,该流域气象站点分布稀疏,水文过程研究存在很大的局限性,研究多种降水数据产品在流域的适用性对水文模型研究具有重要的推动作用。本文以黄河源区作为研究区域,基于中国气象同化驱动数据集(China Meteorol... 详细信息

黄河源区位于青藏高原的东北部,该流域气象站点分布稀疏,水文过程研究存在很大的局限性,研究多种降水数据产品在流域的适用性对水文模型研究具有重要的推动作用。本文以黄河源区作为研究区域,基于中国气象同化驱动数据集(China Meteorological Assimilation Datasets for SWAT model Version1.1,CMADS V1.1)、热带降雨测量卫星(Tropical Rainfall Measurement Mission,TRMM)降水数据集(3B42 Version7)和气象站观测降水数据分别驱动土壤水文评估工具分布式水文模型(Soil and Water Assessment Tool,SWAT),同时利用SWAT-CUP(SWAT Calibration and Uncertainty Program)和SUFI-2(Sequential Uncertainty Fitting2)算法对27个敏感性参数进行率定,模拟黄河源区多年月平均径流量的变化规律,并将模拟结果与观测值进行对比分析,评估了CMADS和TRMM 3B42降水数据产品在该流域的精度以及SWAT模型在黄河源区的适用性。结果表明:(1)由3种降水数据获得的降水空间分布为由西向东递增的趋势,TRMM 3B42与实测降水在年和月变化上一致性好于CMADS数据集。(2)参数敏感性分析结果表明:SCS(Soil Conservation Service)径流曲线数、地下水滞后系数、土壤蒸发补偿系数对径流模拟的敏感性程度较强。(3)利用CMADS和TRMM 3B42降水数据集模拟的径流结果均优于实测降水数据,流域内3个水文站在率定期的相关系数R分别是0.93,0.92和0.88;TRMM 3B42模拟结果次之,率定期和验证期的相关系数R均在0.80以上,纳什系数(Nash-Sutcliffe efficiency coefficient,NSE)在0.50以上。本研究证明了CMADS数据集和SWAT模型在地貌类型复杂、气候变化敏感的高海拔地区的径流模拟方面具有较好的适用性,为气象站点稀缺的地区提供了建立水文模型的替换方案。

关键词：黄河源区 SWAT水文模型径流模拟再分析降水数据

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三黑杂粮营养馒头最佳配方研究

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粮食加工 2024年第2期49卷 54-58页

作者：罗文李奕萱曾珍鲁璐龙文玲四川旅游学院成都610100 旌晶食品有限公司四川德阳618000

在传统白面馒头的制作基础上,通过添加黑米粉、膨化黑豆粉和熟黑芝麻粉研制三黑杂粮营养馒头。在单因素试验基础上选取试验因素和水平,根据Box-Benhnken的试验设计原理,采用三因素三水平的响应面分析法获得最佳配方。结果表明:以小麦粉1... 详细信息

在传统白面馒头的制作基础上,通过添加黑米粉、膨化黑豆粉和熟黑芝麻粉研制三黑杂粮营养馒头。在单因素试验基础上选取试验因素和水平,根据Box-Benhnken的试验设计原理,采用三因素三水平的响应面分析法获得最佳配方。结果表明:以小麦粉100 g为基准,黑米粉添加量20%、膨化黑豆粉添加量4%、熟黑芝麻粉添加量10%为最优值。此条件下制成的馒头色泽黑亮、质地暄软、口味香浓,市场应用前景良好。

关键词：黑米粉膨化黑豆粉熟黑芝麻粉响应面分析营养馒头

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