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Establishing a protein expression profile database for the normal human pituitary gland using two-dimensional high-performance liquid chromatography combined with LTQ-Orbitrap mass spectrometry

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Neural Regeneration Research 2012年第36期7卷 2922-2928页

作者： Rong Xie Wei Xu Weimin Bao Hang Liu Luping Chen yiwen shen Jianhong Zhu Department of Neurosurgery Huashan HospitalFudan University Institution of Biological Medicine Fudan University

In this study, we selected adult normal pituitary gland tissues from six patients during operations for pituitary microadenomas via the transsphenoidal approach for extended normal pituitary tissue resection around the tumor, and analyzed the protein expression of human normal pituitary using two-dimensional high-performance liquid chromatography combined with LTQ-Orbitrap mass spectrometry proteomics technology. The ten most highly expressed proteins in normal human pituitary were： alpha 3 type VI collagen isoform 5 precursor （abundance among tall pituitary proteins 1.30%）, fibrinogen beta chain preproprotein （0.99%）, vimentin （0.73%）, prolactin （0.69%）, ATP synthase, H~ transporting and mitochondrial F1 complex beta subunit precursor （0.52%）, keratin I （0.49%）, growth hormone （0.45%）, carbonic anhydrase I （0.40%）, heat shock protein 90 kDa I （0.31%）, and annexin V （0.30%）. Based on the biological function classifications of these proteins, the top three categories by content were neuroendocrine proteins （abundance among all pituitary proteins, 40.1%）, catalytic and metabolic proteins （28.3%）, and cell signal transduction proteins （9.8%）. Based on cell positioning classification, the top three categories were cell organelle （24.5%） membrane （20.8%）, and cytoplasm （13.0%）. Based on biological process classification, the top three categories of proteins are involved in physiological processes （42.9%）, cellular processes （40.4%）, and regulation of biological processes （9.1%）. Our experimental findings indicate that a protein expression profile database of normal human pituitary can be precisely and efficiently established by proteomics technology.

关键词： two-dimensional high-performance liquid chromatography mass spectrum pituitary gland proteins proteomics hypophyseal tumor physiological function pathological mechanism

Erratum to“Gravity-Dependent Animacy Perception in Zebrafish”

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Research 2024年第3期6卷 799-800页

作者： Xiaohan Ma Xiangyong Yuan Jiahuan Liu Li shen yiwen Yu Wen Zhou Zuxiang Liu Yi Jiang State Key Laboratory of Brain and Cognitive Science CAS Center for Excellence in Brain Science and Intelligence TechnologyInstitute of PsychologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Chinese Institute for Brain Research Beijing 102206China State Key Laboratory of Brain and Cognitive Science Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefei 230088China

In the Research Article“Gravity-Dependent Animacy Perception in Zebrafish”[1],there was an error when calculating the velocity and acceleration of the human and pigeon BM stimuli provided by other researchers in ***... 详细信息

关键词： acceleration finding Zebrafish

High-Value-Added Utilization of Turpentine:Screening of Anti-Influenza Virus Agents fromβ-Pinene Derivatives

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Journal of Renewable Materials 2024年第1期12卷 45-56页

作者： yiwen Li Hongyan Si Peng Wang Hai Luo Minggui shen Xiaoping Rao Zhanqian Song Shibin Shang Zongde Wang shengliang Liao East China Woody Fragrance and Flavor Engineering Research Center of National Forestry and Grassland Administration Camphor Engineering Research Center of National Forestry and Grassland AdministrationCollege of ForestryJiangxi Agricultural UniversityNanchang330045China Institute of Chemical Industry of Forestry Products China Academy of ForestryNational Engineering Laboratory for Biomass Chemical UtilizationKey and Open Laboratory of Forest Chemical EngineeringState Forestry AdministrationKey Laboratory of Biomass Energy and MaterialJiangsu ProvinceNanjing210042China College of Chemical Engineering Huaqiao UniversityXiamen361021China

Turpentine is a renewable and resourceful forest *** deep processing and utilization of turpentine,particularly its primary componentβ-pinene,has garnered widespread *** study aimed to synthesize 40 derivatives ofβ-pinene,including nopinone,3-cyanopyridines of nopinone,myrtanyl acid,myrtanyl acylthioureas,and myrtanyl *** assessed the antiviral activities of theseβ-pinene derivatives against influenza virus A/Puerto Rico/8/34(H1N1)using the 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ***β-pinene derivatives were used before and after cellular infection with the influenza virus to evaluate their preventive and therapeutic effects against the H1N1 *** results showed that only compound 10o exhibited a preventive effect against the H1N1 virus with a half-maximal inhibitory concentration(IC50)value of 47.6μmol/*** the compounds,4e,4i,and 4l demonstrated therapeutic effects against cellular infection,with compound 4e displaying the most potent therapeutic effect(IC50=17.5μmol/L),comparable to the positive control *** findings indicated that certainβ-pinene derivatives exhibited in vitro antiviral activity against the H1N1 influenza A virus,warranting further investigation as potential anti-influenza agents.

关键词： Antiviral activity β-pinene derivative 3-cyanopyridine influenza virus

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高校科技成果转化新问题与对策再思考

科技管理研究 2011年第13期31卷 82-84页

作者：沈意文扬州大学人文社科处江苏扬州225009

高校科技成果转化率不高引起了社会各界的广泛关注,对当前高校科技成果转化存在的问题和原因进行了分析和思考,并在此基础上提出了促进高校科技成果转化的新思路。

关键词：高校科技成果转化思考

Discovery and characterization of naturally occurring covalent inhibitors of SARS-CoV-2 M^(pro)from the antiviral herb Ephedra

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Chinese Journal of Natural Medicines 2024年第9期22卷 797-807页

作者： HU Qing ZHANG yiwen CHEN Pengcheng ZHANG Yani ZHU Guanghao LIU Wei WANG Chaoran ZHENG Shuilian shen Nonger WANG Haonan HUANG Ping GE Guangbo Center for Clinical Pharmacy Cancer CenterDepartment of PharmacyZhejiang Provincial People’s Hospital(Affiliated People’s Hospital)Hangzhou Medical CollegeHangzhou 310014China Shanghai Frontiers Science Center of TCM Chemical Biology Institute of Interdisciplinary Integrative Medicine ResearchShanghai University of Traditional Chinese MedicineShanghai 201203China Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine Shanghai 200021China Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023China

The Chinese herb Ephedra(also known as Mahuang)has been extensively utilized for the prevention and treatment of coronavirus-induced diseases,including coronavirus disease 2019(COVID-19).However,the specific anti-SARS-CoV-2 compounds and mechanisms have not been fully *** main protease(M^(pro))of SARS-CoV-2 is a highly conserved enzyme responsible for proteolytic processing during the viral life cycle,making it a critical target for the development of antiviral *** study aimed to identify naturally occurring covalent inhibitors of SARS-CoV-2 M^(pro)from Ephedra and to investigate their covalent binding *** results demonstrated that the non-alkaloid fraction of Ephedra(ENA)exhibited a potent inhibitory effect against the SARS-CoV-2 M^(pro)effect,whereas the alkaloid fraction did ***,the chemical constituents in ENA were identified,and the major constituents'anti-SARS-CoV-2 M^(pro)effects were *** the tested constituents,herbacetin(HE)and gallic acid(GA)were found to inhibit SARS-CoV-2 M^(pro)in a time-and dose-dependent *** combination displayed a significant synergistic effect on this key ***,various techniques,including inhibition kinetic assays,chemoproteomic methods,and molecular dynamics simulations,were employed to further elucidate the synergistic anti-M^(pro)mechanisms of the combination of HE and ***,this study deciphers the naturally occurring covalent inhibitors of SARS-CoV-2 M^(pro)from Ephedra and characterizes their synergistic anti-M^(pro)synergistic effect,providing robust evidence to support the anti-coronavirus efficacy of Ephedra.

关键词： Main protease Ephedra Ephedra non-alkaloid fraction Covalent inhibitors Synergistic effect

Unveiling the secrets of diatom-mediated calcification:Implications for the biological pump

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Science China Earth Sciences 2024年第9期67卷 2895-2909页

作者： yiwen PAN Yifan LI Chen-Tung Arthur CHEN Zong-Pei JIANG Wei-Jun CAI Yunwen shen Zesheng DING Qixian CHEN Yanan DI Wei FAN Chenba ZHU Ying CHEN Ocean College Zhejiang UniversityZhoushan 316021China Hainan Institute of Zhejiang University Sanya 570203China Department of Oceanography Sun Yat-Sen UniversityGaoxiong 80424China School of Marine Science and Policy University of DelawareNewark DE 19716USA Carbon Neutral Innovation Research Center Xiamen UniversityXiamen 361005China

Siliceous diatoms are one of the most prominent actors in the oceans,and they account for approximately 40%of the primary production and particulate organic carbon export *** is believed that changes in carbon flux caused by variations in diatom distribution can lead to significant climate *** the fundamental pathways of diatom-driven carbon sequestration have long been established,there are no reports of CaCO_(3) precipitation induced by marine diatom *** manuscript introduces novel details regarding the enhancement of aragonite precipitation during photosynthesis in Skeletonema costatum in both artificial and natural *** direct measurements of cell surfaces via a pH microelectrode and zeta potential analyzer,it was determined that the diatom-mediated promotion of CaCO_(3) precipitation is achieved through the creation of specific microenvironments with concentrated[CO_(3)^(2-)]and[Ca^(2+)]and/or the dehydrating effect of adsorbed Ca^(2+).Based on this mechanism,it is highly plausible that diatom-mediated calcification could occur in the oceans,an assertion that was supported by the significant deviation of total alkalinity(TA)from the conservative TA-salinity mixing line during a Skeletonema costatum bloom in the East China Sea and other similar *** newly discovered calcification pathway establishes a link between particulate inorganic and organic carbon flux and thus helps in the reassessment of marine carbon export fluxes and CO_(2) sequestration *** discovery may have important ramifications for assessing marine carbon cycling and predicting the potential effects of future ocean acidification.

关键词： Diatom bloom Skeletonema costatum Calcification Biological carbon pump Counter carbonate pump

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科技人力资源能力建设:概念与思考

高等工程教育研究 2007年第5期55卷 10-15页

作者：沈漪文孔寒冰浙江大学科教发展战略研究中心

科技人力资源(HRST)被看作竞争和经济发展的最核心要素,是国家和地区发展的第一战略资源,开发和利用这个宝贵资源是我们的紧迫任务。HRST能力建设则是应运而生的全新概念。本文在讨论"科技人力资源"、"能力"、"能力建设"等概念的基础上... 详细信息

科技人力资源(HRST)被看作竞争和经济发展的最核心要素,是国家和地区发展的第一战略资源,开发和利用这个宝贵资源是我们的紧迫任务。HRST能力建设则是应运而生的全新概念。本文在讨论"科技人力资源"、"能力"、"能力建设"等概念的基础上,探讨科技人力资源能力建设的内涵,强调此种能力建设对工程教育的意义。

关键词：科技人力资源能力建设能力

抗生素及其抗性基因在环境中的污染、降解和去除研究进展

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应用与环境生物学报 2015年第2期21卷 181-187页

作者：沈怡雯黄智婷谢冰华东师范大学生态与环境科学学院上海市城市化生态过程与生态恢复重点实验室上海200241

随着抗生素及其抗菌产品的广泛应用,自然和人工环境中的抗生素残留带来的危害引起人们关注.本文基于最新文献,综述了国内外抗生素及其抗性基因的污染水平和来源、它们之间的关系和传播机理以及这类污染物的降解和去除技术.现有研究表明... 详细信息

随着抗生素及其抗菌产品的广泛应用,自然和人工环境中的抗生素残留带来的危害引起人们关注.本文基于最新文献,综述了国内外抗生素及其抗性基因的污染水平和来源、它们之间的关系和传播机理以及这类污染物的降解和去除技术.现有研究表明,抗生素及其抗性基因的污染已遍布水、土壤、大气等介质,而在以污水处理厂和固废填埋场为代表的人工环境中,其污染水平更高.抗生素残留诱导产生抗性基因,其在环境中传播扩散与水平基因转移(Horizontal Gene Transfer,HGT)和微生物群落结构组成有关.抗生素和抗性基因在环境中自然降解过程受基质类型、光照、温度和微生物种群等因素的影响,其中光照是影响其降解的重要因子;而在人工处理系统中,紫外消毒和生化降解对抗生素及其抗性基因有较好的去除效果,但并非全部有效.建议今后加强对特定环境中抗生素和抗生素抗性基因的扩散规律和高效降解去除等方面的机理和工艺研究,进而有效控制其环境含量,降低其污染水平.

关键词：抗生素抗性基因水平基因转移微生物群落结构降解机理去除效果

结合Transformer的轻量化中文语音识别

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计算机应用研究 2023年第2期40卷 424-429页

作者：沈逸文孙俊江南大学人工智能与计算机学院江苏无锡214122

近年来,深度神经网络模型在语音识别领域成为热门研究对象。然而,深层神经网络的构建依赖庞大的参数和计算开销,过大的模型体积也增加了其在边缘设备上部署的难度。针对上述问题,提出了基于Transformer的轻量化语音识别模型。首先使用... 详细信息

近年来,深度神经网络模型在语音识别领域成为热门研究对象。然而,深层神经网络的构建依赖庞大的参数和计算开销,过大的模型体积也增加了其在边缘设备上部署的难度。针对上述问题,提出了基于Transformer的轻量化语音识别模型。首先使用深度可分离卷积获得音频特征信息;其次构建了双半步剩余权重前馈神经网络,即Macaron-Net结构,并引入低秩矩阵分解,实现了模型压缩;最后使用稀疏注意力机制,提升了模型的训练速度和解码速度。为了验证模型,在Aishell-1和aidatatang_200zh数据集上进行了测试。实验结果显示,与Open-Transformer相比,所提模型在字错误率上相对下降了19.8%,在实时率上相对下降了32.1%。

关键词：语音识别 Transformer 低秩矩阵分解轻量卷积模型压缩稀疏注意力