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Screening Helicobacter pylori genes induced during infection of mouse stomachs

World Journal of Gastroenterology 2012年第32期18卷 4323-4334页

作者： Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood yisheng ni Kimberly Smith Sharon H Jackson William G Coleman Laboratory of Biochemistry and Genetics National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda MD 20892 United States Laboratory of Host Defenses National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda MD 20892 United States

AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(***) as it relates to its survival in the ***:In vivo expression technology(IVET) systems are used to identify microbial virulence *** modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and *** novel IVET systems were developed by the fusion of random Sau3A DNA fragments of *** and a tandem-reporter system of chloramphenicol acetyltransferase and ***,each vector contains a kanamycin resistance *** used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that *** expresses in *** expression studies were conducted by infecting RAW 264.7 cells with *** was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced ***:In this study,we have identified 31 in vivo induced(ivi) genes in the initial *** 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse *** factors,vacA and cagA,were found in this screen and are known to play important roles in *** infection,colonization and *** detection validates the efficacy of these screening *** of the identified ivi genes have already been implicated to play an important role in the pathogenesis of *** and other bacterial pathogens such as Escherichia coli and Vibrio *** profiles of allivi genes were confirmed by real time PCR analysis of *** RNA isolated from *** infected RAW 264.7 *** compared the expression profile of *** and RAW 264.7 coculture with that of *** *** ge

关键词： Helicobacter pylori In vivo expression technology Virulence genes Mice Infection

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倪氏源流考

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寻根 2006年第1期 110-114页

作者：倪义省

2002年夏,倪国(即小邾国)国王友的陵墓在山东省枣庄市山亭区东江村的成功发掘,揭开了倪国一段鲜为人知的历史。早已湮灭在历史长河中的这段故事,重新引起了世人的注意,倪国和倪氏渊源关系的谜底也因之初步揭开。同中华民族的其他“百姓... 详细信息

2002年夏,倪国(即小邾国)国王友的陵墓在山东省枣庄市山亭区东江村的成功发掘,揭开了倪国一段鲜为人知的历史。早已湮灭在历史长河中的这段故事,重新引起了世人的注意,倪国和倪氏渊源关系的谜底也因之初步揭开。同中华民族的其他“百姓”一样,倪氏始祖也是黄帝。再具体一点说,?（共5页)

关键词：源流考渊源关系山亭区山东省历史序

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Study of Methanol Conversion over Fe-Zn-Zr Catalyst

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Journal of Natural Gas Chemistry 2007年第3期16卷 326-328页

作者： Xiaoming ni yisheng Tan Yizhuo Han State Key Laboratory of Coal Conversion Institute of Coal Chemistry Chinese Academy of SciencesTaiyuan 030001 Shanxi China Huaibei Coal Industry Teachers College Huaibei 235000 Anhui China

The methanol conversion over Fe-Zn-Zr catalyst was studied at 0.1 MPa and 280-360 ℃ The experimental results indicate that the main products of methanol conversion are methane and butane, and that other hydrocarbons are scarcely produced. All results show that propylene is most probably the olefin formed first in methanol conversion rather than ethene over Fe-Zn-Zr catalyst. Methane is formed from methoxy group, and C4 is possibly yielded on the surface from propylene through binding with a methoxy group.

关键词： methanol conversion Fe-Zn-Zr catalyst

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ACTIVE PRODUCTS OF PORCINE TRYPSIN AFTER AUTOLYSIS

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Science in China,Ser.A 1980年第11期 1453-1460页

作者：茹炳根杜锦珠曾耀辉陈濂生倪逸声谭国华张龙翔 Department of Biology Beijing University

Porcine trypsin obtained from pancreas residues subsequent to insulin removal undergoes autolysis when subjected to chromatography and gives rise to new active forms of autolyzed products with intra-chain split at bonds Lys145—Ala146 and Arg105—*** of 1% solutions of porcine trypsin either at pH 5.0 or at pH 9.1 induces autolysis to give active products involving one or two specific cleavages of bonds Lys145—Ala148 and Arg105—Val106 or Lys131—Ser132, as well as inactive degraded products, No evidence has been obtained that on autolysis of porcine trypsin, any active fragment with molecular weight lower than that of the parent molecule was *** active forms of autolyzed products of porcine trypsin have almost the same specific activity as the intact enzyme when assayed against BAEE. They are of the same molecular weight as the parent molecule. These findings indicate that the active forms of autolyzed products maintain the specific three-dimensio

关键词： ACTIVE PRODUCTS OF PORCINE TRYPSIN AFTER AUTOLYSIS very NH

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Influence of zirconium addition on the microstructure and magnetic properties of nanocomposite Nd_(10.1)Fe_(78.2-x)Co_5Zr_xB_(6.7) permanent magnets

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Rare Metals 2007年第3期26卷 218-221页

作者： FENG Shanshan ni Jiansen WANG Zhanyong WU yisheng ZHOU Bangxin XU Hui Institute of Materials Science Shanghai University Shanghai 20007 China

Nanocomposite Nd10.1Fe78.2-xCo5ZrxB6.7 （x= 0, 1.5, 2.5, 2.7, 3, 4） permanent magnets were prepared by melt-spun and annealing. The microstructure and magnetic properties of the permanent magnets were investigated. The resuits reveal that the addition of Zr element significantly reduces the grain size and improves the thermal stability of the amorphous phase. A fme nanocomposite microstructure with an average grain size of about 35 nm can be developed at a wheel speed of 16 m·s^-1 with the content of Zr up to 2.7 at.%. After optimal annealing （710℃ x 4 min）, the magnetic properties of the ***6.7 bonded magnets were achieved as follows： Br= 0.72 T, jHc = 769 kA·m^-1, and （BH）max = 85.0 kJ·m^-3.

关键词： magnetic properties Nd-Fe-B nanocomposite rare earths bonded magnet

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DDT INDUCTION OF TYROSINE DECARBOXYLASE IN DDT-POISONED COCKROACHES(Ⅰ)

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Chinese Science Bulletin 1984年第1期29卷 141-页

作者：罗远倪逸声张宗炳 Departmens of Biology Peking University

We have reported that the neurotoxin released by DDT-poisoned cockroaches was identified to be tyramine, the decarboxylated product of tyrosine. The excessive production of this neurotoxin is apparently the result of ... 详细信息

关键词： apparently released induction 伪口 letter

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Polymeric assembled nanoparticles through kinetic stabilization by confined impingement jets dilution mixer for fluorescence switching imaging

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Chinese Journal of Chemical Engineering 2023年第4期56卷 89-96页

作者： Jingran Liu Yue Wu Jie Tang Tao Wang Feng ni Qiumin Wu Xijiao Yang Ayyaz Ahmad Naveed Ramzan yisheng Xu State Key Laboratory of Chemical Engineering East China University of Science and TechnologyShanghai 200237China Shanghai Shyndec Pharmaceutical Co. LtdShanghai 200040China Department of Chemical Engineering Muhammad Nawaz Sharif University of Engineering and TechnologyMultanPakistan Faculty of Chemical Metallurgicaland Polymer EngineeringUniversity of Engineering&TechnologyLahorePakistan

Traditional fluorescence switching molecules achieving the state change between on and off states commonly based on UV irradiation. However, it is worth noting that UV irradiation is harmful to both the cancer cells and the normal cells. To achieve fluorescence switching under visible wavelength and avoid complicate molecular design, a fluorophore of 2,4,5,6-tetrakis(carbazol-9-yl)-1,3-dicyanobenzene(4Cz IPN) and a quencher of diarylethene(DAE) were physically incorporated within the biocompatible block copolymer poly(lactic-co-glycolic acid)-b-poly(ethylene glycol)(PLGA-b-PEG) to form 4Cz IPNDAE nanoparticles(NPs) through flash nanoprecipitation(FNP). By using the FNP method, the NPs were prepared within milliseconds in a confined impingement jets dilution(CIJ-D) mixer. Quenching and recovery of fluorescence could achieve in the presence of DAE under 475 nm and 560 nm *** structure and fluorescent properties of the nanoparticles can be tuned by external conditions for their efficient fluorescence resonance energy transfer(FRET) in a kinetic stabilization process. This NPs formation process was further optimized by varying the dilution ratio, Reynolds number(Re) and polymer concentration to modulate the mixing and particle nucleation and growth process. The size and fluorescence switching properties of the NPs were systematically investigated in solution and in cellular uptake experiments. This work is anticipated to provide a simple and highly effective engineering strategy for the modulation of fluorescence switching nanoparticles and beneficial to its engineering application.

关键词： Preparation Kinetic stabilization Flash nanoprecipitation Nanoparticles Fluorescence switch

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Methanation of syngas over coral reef-like ni/Al_2O_3 catalysts

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Journal of Natural Gas Chemistry 2011年第4期20卷 435-440页

作者： Shengli Ma yisheng Tan Yizhuo Han State Key Laboratory of Coal Conversion Institute of Coal Chemistry Chinese Academy of Sciences Taiyuan 030001 Shanxi China Graduate University of Chinese Academy of Sciences Beijing 100039 China

Coral reef-like ni/Al2O3 catalysts were prepared by co-precipitation of nickel acetate and aluminium nitrate with sodium carbonate aqueous solution in the medium of ethylene *** of syngas was carried out over coral reef-like ni/Al2O3 catalysts in a continuous flow type fixed-bed *** structure and properties of the fresh and used catalysts were studied by SEM,N2 adsorption-desorption,XRD,H2-TPR,O2-TPO,TG and ICP-AES *** results showed that the coral reef-like ni/Al2O3 catalysts exhibited better activity than the conventional ni/Al2O3-H2O *** activities of coral reef-like catalysts were in the order of ni/Al2O3-673ni/Al2O3-573ni/Al2O3- 473ni/***/Al2O3-673-EG catalyst showed not only good activity and improved stability but also superior resistance to carbon deposition,sintering,and ni *** the reaction conditions of CO/H2（molar ratio）=1：3,593 K,atmospheric pressure and a GHSV of 2500 h-1,CH4 selectivity was 84.7%,and the CO conversion reached 98.2%.

关键词： methanation syngas coral reef-like ni/Al2O3 catalysts calcination

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Catalytic Oxidation of Dimethyl Ether to Dimethoxymethane over Cs Modified H_3PW_(12)O_(40)/SiO_2 Catalysts

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Journal of Natural Gas Chemistry 2007年第3期16卷 322-325页

作者： Qingde Zhang yisheng Tan Caihong Yang Yizhuo Han Jun Shamoto Noritatsu Tsubaki State Key Laboratory of Coal Conversion Institute of Coal Chemistry Chinese Academy of Sciences Taiyuan 030001 Shanxi China Graduate University of Chinese Academy of Sciences Beijing 100049 China Department of Applied Chemistry University of Toyama Toyama 930-8555 Japan

The attractive utilization route for one-step catalytic oxidation of dimethyl ether to dimethoxymethane was successfully carried out over the H3PW12O40（40%）/SiO2 catalyst, modified by Cs, K, ni, and V. The Cs modification of H3PW12O40（40%）/SiO2 gave the most promising result of 20% dimethyl ether conversion and 34.8% dimethoxymethane selectivity. Dimethoxymethane could be synthe- sized via methoxy groups decomposed from dimethyl ether through the synergistic effect between the acid sites and the redox sites of Cs modified H3PW12O40（40%）/SiO2.

关键词： dimethyl ether dimethoxymethane catalytic oxidation Cs modified H3PW12O40/SiO2 XRD

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二氧化碳催化转化的研究进展

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石油化工 2005年第6期34卷 505-512页

作者：倪小明谭猗生韩怡卓中国科学院山西煤炭化学研究所煤转化国家重点实验室

对近几年关于CO2的催化转化生成甲醇、二甲醚、烃类、合成气等基础化工原料以及转化为以碳酸二甲酯为代表的酯类、羧酸、N-甲酰苯胺、各种聚合物等多种具有高附加值的产品的新催化合成过程的研究与开发进行了评述,并对CO2催化转化领域... 详细信息

对近几年关于CO2的催化转化生成甲醇、二甲醚、烃类、合成气等基础化工原料以及转化为以碳酸二甲酯为代表的酯类、羧酸、N-甲酰苯胺、各种聚合物等多种具有高附加值的产品的新催化合成过程的研究与开发进行了评述,并对CO2催化转化领域的研究发展方向进行了初步的探讨。

关键词：二氧化碳催化转化

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