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哈氏弧菌345Ⅲ型分泌系统诱导鱼类细胞凋亡和变圆

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微生物学通报 2024年第3期51卷 779-791页

作者：吴颖邓益琴张策李席席张晨梁思轩徐雪峰赵哲河海大学江苏省海洋生物资源可持续利用工程研究中心江苏南京210024 河海大学海洋学院海洋生物系江苏南京210024 中国水产科学研究院南海水产研究所农业农村部南海渔业资源开发利用重点实验室广东广州510300

【背景】哈氏弧菌(Vibrio harveyi)是一种革兰氏阴性细菌,也是海洋动物的重要条件致病菌之一。Ⅲ型分泌系统(typeⅢsecretionsystem,T3SS)是革兰氏阴性致病菌中保守存在的毒力装置,已被公认在细菌致病过程中起着关键作用。【目的】探索... 详细信息

【背景】哈氏弧菌(Vibrio harveyi)是一种革兰氏阴性细菌,也是海洋动物的重要条件致病菌之一。Ⅲ型分泌系统(typeⅢsecretionsystem,T3SS)是革兰氏阴性致病菌中保守存在的毒力装置,已被公认在细菌致病过程中起着关键作用。【目的】探索哈氏弧菌T3SS诱导鱼类细胞死亡的机制。【方法】采用基因敲除和回补手段获得了T3SS功能缺失的哈氏弧菌345的缺失突变和回补株。利用4’,6-二脒基-2-苯基吲哚(4′,6-diamidino-2-phenylindole,DAPI)染色、原位末端转移酶标记技术(TdT-mediated dUTP nick-end labeling, TUNEL)、含半胱氨酸的天冬氨酸蛋白水解酶(cysteinyl aspartatespecific proteinase, Caspase)活性测定和lactate dehydrogenase (LDH)释放测定等技术揭示哈氏弧菌T3SS导致的细胞死亡特征。【结果】哈氏弧菌345依赖T3SS能迅速导致鱼类细胞变圆并脱落,细胞核皱缩,部分细胞呈现DNA片段化、形成凋亡小体,细胞凋亡关键分子Caspase-3被激活;此外,哈氏弧菌T3SS还导致鱼类细胞的细胞膜通透性改变,LDH等内容物被释放,并且抑制凋亡途径的激活并不能阻止细胞内容物的释放和细胞变圆。【结论】哈氏弧菌利用T3SS体外诱导鱼类细胞发生细胞凋亡途径的激活和细胞变圆,最终导致细胞发生死亡。

关键词：哈氏弧菌 Ⅲ型分泌系统 vcrD基因细胞凋亡

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Effects of seagrass leaf litter decomposition on sediment organic carbon composition and the key transformation processes

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Science China Earth Sciences 2017年第12期60卷 2108-2117页

作者： LIU SongLin JIANG ZhiJian deng yiqin WU YunChao ZHAO ChunYu ZHANG JingPing SHEN Yuan HUANG XiaoPing Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of Sciences College of Earth Science University of Chinese Academy of Sciences South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences

Seagrass leaf litters are an important source of sediment organic carbon(SOC). However, the mechanisms of seagrass leaf litter decomposition influencing SOC composition and the key transformation processes remain unknown. We performed a laboratory chamber experiment to compare the labile organic carbon(OC) composition and the enzyme activities governing SOC transformation between the seagrass group(seagrass leaf litter addition) and the control group. The results showed that the seagrass leaf litter decomposition significantly elevated the salt-extractable carbon(SEC) content and the SEC/SOC. Additionally,the levels of invertase, polyphenol oxidase, and cellulase in the seagrass leaf litters addition group were generally higher than in the control group, which could elevate recalcitrant OC decomposition. Following 24 days incubation, addition of seagrass leaf litter increased the amount of CO_2 released, but decreased the SOC content. Therefore, seagrass leaf litter decomposition leached abundant dissolved OC, which enhanced the activity and transformation of SOC.

关键词： Seagrass leaf litter Sediment organic carbon Composition Enzyme activities

Analysis of the Causes of the Intensity Enhancement of the Typhoon “Tiange”

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Journal of Geoscience and Environment Protection 2023年第7期11卷 156-164页

作者： yiqin deng Xin Qi Zhewei deng Rong Huang Pingtan Comprehensive Experimental Area Meteorological Bureau Pingtan China Qingliu County Meteorological Bureau Sanming China Fuzhou Meteorological Bureau Fuzhou China

This paper analyzes the causes of the offshore strength enhancement of Typhoon Tiange (No. 1713) from four aspects: high and low layer divergence, vertical wind shear, southwest monsoon transport, offshore sea temperature and weak cold air. The conclusions are as follows: The high-level airflow divergence enhancement and positive vorticity advection in the offshore, strengthen the middle rise movement of the “Tiange”, and the ascending movement enhances the strength of the “Tiange” by strengthening the lower layer convergence;The high offshore sea temperature and sea temperature are changing temperature, which also has a certain effect on the strengthening of “Tiange” in the offshore;The strengthening of the southwest warm and humid airflow provides sufficient water vapor and energy for the development of “Tiange”, which is beneficial to the maintenance of the typhoon warm heart structure;The intrusion of weak cold air, enhanced the radiation cooperation of the lower typhoon disturbance, which is beneficial to the enhancement of “Tiange”.

关键词： Typhoon Offshore Strengthening Cause Analysis

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水产动物弧菌病及其生物防治研究进展

大连海洋大学学报 2023年第4期38卷 553-563页

作者：邓益琴中国水产科学研究院南海水产研究所农业农村部南海渔业资源开发利用重点实验室广东广州510300

弧菌病是水产养殖动物主要细菌病之一,对鱼、虾、蟹和贝类等均会造成危害,研究病害防治技术对推动水产养殖可持续发展具有重要意义。弧菌病防治手段主要有以抗生素为代表的药物防治、以疫苗为代表的免疫防治及以益生菌为代表的生物防治... 详细信息

弧菌病是水产养殖动物主要细菌病之一,对鱼、虾、蟹和贝类等均会造成危害,研究病害防治技术对推动水产养殖可持续发展具有重要意义。弧菌病防治手段主要有以抗生素为代表的药物防治、以疫苗为代表的免疫防治及以益生菌为代表的生物防治。其中,生物防治是利用物种间的相互关系,以一种或一类生物或其衍生物抑制另一种或另一类生物的方法,其可在养殖全过程进行疾病的广谱预防和控制,且对环境和食品安全影响小,是具有巨大潜力的防治技术。目前,水产养殖中弧菌病的生物防治手段主要有益生菌及其相关产品、药用植物和噬菌体。本文在总结水产动物弧菌病病原学、流行病学、发病机制和诊断方法的基础上,主要从防治技术的种类、机制和应用等方面综述了水产养殖动物弧菌病的生物防治研究进展,并提出在深入探究生物防治作用机制、作用模式的同时,确保环境友好并高质高效推动其商业化发展等未来重点研究方向,以期为水产养殖动物弧菌病的生物防治研究提供科学参考。

关键词：水产动物弧菌病生物防治防治机制

Metabolism of branched-chain amino acids revealed by transcriptome analysis in Vibrio alginolyticus

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Metabolism of branched-chain amino acids revealed by transcr...

Identification of a novel sRNA to switch the flux of alanine into carbon and nitrogen metabolism pathways in Vibrio alginolyticus

第五届全国人畜共患病学术研讨会

作者： yiqin deng Xiongqi Ding Xiaochun Huang Yiying Yang Chang Chen Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of Sciences

Branched-chain amino acids(BCAAs)play important roles in nitrogen ***,little is known about the metabolism of BCAAs in the fish pathogen Vibrio *** this study,the global gene expression patterns of *** ZJ-T cultured in M63 minimal medium supplemented with ammonium sulfate or with three BCAAs(isoleucine,leucine and valine)as nitrogen source were evaluated by transcriptome *** results revealed that 311

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Identification of a novel sRNA to switch the flux of alanine...

Inhibition of xanthine oxidase alleviated pancreatic necrosis via HIF-1α-regulated LDHA and NLRP3 signaling pathway in acute pancreatitis

第五届全国人畜共患病学术研讨会

作者： yiqin deng Chang Chen Annick Jacq Xiaochun Huang Yiying Yang Xiongqi Ding Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of Sciences Institute for Integrative Biology of the Cell(I2BC) CEACNRSUniversite Paris-SudUniversite Paris-Saclay

Amino acids(AAs)are the major nutrients that can serve both as nitrogen and carbon sources for bacteria growth,hence their metabolisms are strictly regulated by the availability of carbon and nitrogen in the *** a novel sRNA,amR(Alanine Metabolism Regulator),was identified and found to regulate alanine metabolism in Vibrio *** 5’and 3’-RACE,the boarder of amR transcript was *** whole sequence is 313nt and a rho-independant

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3591-3604页

作者： Juan Rong Chenxia Han Yan Huang yiqin Wang Qi Qiu Manjiangcuo Wang Shisheng Wang Rui Wang Juqin Yang Xia Li Chenggong Hu Zhiyao Chen Lihui deng Wei Huang Qing Xi Dan Du West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western MedicineWest China Hospital/West China Medical SchoolSichuan UniversityChengdu 610041China State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Advanced Mass Spectrometry Center Research Core FacilityFrontiers Science Center for Disease-related Molecular NetworkWest China HospitalSichuan UniversityChengdu 610213China Proteomics-Metabolomics Platform Research Core FacilityInstitutes for Systems GeneticsWest China HospitalSichuan UniversityChengdu 610093China Biobank West China HospitalSichuan UniversityChengdu 610093China West China School of Nursing Sichuan UniversityChengdu 610041China Department of Critical Care Medicine West China HospitalSichuan UniversityChengdu 610041China

Acute pancreatitis(AP)is a potentially fatal condition with no targeted treatment *** inhibiting xanthine oxidase(XO)in the treatment of AP has been studied in several experimental models and clinical trials,whether XO is a target of AP and what its the main mechanism of action is remains ***,we aimed to re-evaluate whether XO is a target aggravating AP other than merely generating reactive oxygen species that trigger *** first revealed that XO expression and enzyme activity were significantly elevated in the serum and pancreas of necrotizing AP *** also found that allopurinol and febuxostat,as purine-like and non-purine XO inhibitors,respectively,exhibited protective effects against pancreatic acinar cell death in vitro and pancreatic damage in vivo at different doses and treatment time ***,we observed that conditional Xdh overexpression aggravated pancreatic necrosis and *** mechanism analysis showed that XO inhibition restored the hypoxia-inducible factor 1-alpha(HIF-1α)-regulated lactate dehydrogenase A(LDHA)and NOD-like receptor family pyrin domain containing 3(NLRP3)signaling pathways and reduced the enrichment of^(13)C_(6)-glucose to^(13)C_(3)-***,we observed that clinical circulatory XO activity was significantly elevated in severe cases and correlated with C-reactive protein levels,while pancreatic XO and urate were also increased in severe AP *** results together indicated that proper inhibition of XO might be a promising therapeutic strategy for alleviating pancreatic necrosis and preventing progression of severe AP by downregulating HIF-1α-mediated LDHA and NLRP3 signaling pathways.

关键词： Xanthine oxidase inhibitor Multi-omics HIF-1a Necrotizing acute pancreatitis Lactate Therapeutic target NLRP3 Metabolic flux

我国热带典型海草床潜在致病菌群落结构及其毒力基因丰度的空间特征

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南方水产科学 2020年第5期16卷 1-9页

作者：邓益琴刘松林冯娟江志坚中国水产科学研究院南海水产研究所/广东省渔业生态环境重点实验室/农业农村部南海渔业资源开发利用重点实验室广东广州510300 中国科学院南海海洋研究所热带海洋生物资源与生态重点实验室广东广州510301

该研究利用16S rRNA扩增子测序技术分析了中国热带(新村、黎安、潭门)典型海草床水体潜在致病菌的群落结构,通过选择性培养基分析可培养弧菌和肠球菌的丰度,进一步利用定量PCR分析弧菌(Vibrio)和肠球菌(Enterococcus)典型毒力基因含量... 详细信息

该研究利用16S rRNA扩增子测序技术分析了中国热带(新村、黎安、潭门)典型海草床水体潜在致病菌的群落结构,通过选择性培养基分析可培养弧菌和肠球菌的丰度,进一步利用定量PCR分析弧菌(Vibrio)和肠球菌(Enterococcus)典型毒力基因含量。结果发现,新村、黎安海草床水体潜在致病菌与潭门有显著差异;可培养弧菌和肠球菌及其典型毒力基因vhh、toxR、aspA、esp和gelE均呈现新村>黎安>潭门的趋势,而弧菌典型毒力基因ctxA、tlh、trh和vvp呈现黎安>新村>潭门的趋势。新村和黎安较高的人类活动强度如营养负荷,可能是导致其海草床致病菌和毒力基因丰度较高的重要原因,而新村和黎安的差异可能与不同的人类活动方式有关。

关键词：海草床潜在致病菌弧菌肠球菌典型毒力基因

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从公共艺术谈互动雕塑的发展

艺术探索 2008年第1期22卷 58-58,62页

作者：郭选昌邓义勤重庆大学艺术学院重庆400000

公共艺术是城市空间的主要构成元素之一。雕塑从架上走向了公众与自然,以其更具有参与性的特点成为人和环境不可缺少的交流媒介。城市景观雕塑作品在注重艺术性的同时,更应注重与公众的互动性,增加本身的参与性与创造性,让公众更自觉自... 详细信息

公共艺术是城市空间的主要构成元素之一。雕塑从架上走向了公众与自然,以其更具有参与性的特点成为人和环境不可缺少的交流媒介。城市景观雕塑作品在注重艺术性的同时,更应注重与公众的互动性,增加本身的参与性与创造性,让公众更自觉自愿地参与到艺术中来。互动雕塑在科技的发展下应孕而生,因其独特的互动性、参与性、游戏性,以前所未有的方式进入了公共空间。

关键词：互动雕塑互动性参与性公共艺术