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A transcriptome analysis of two grapevine populations segregating for tendril phyllotaxy

Horticulture Research 2017年第1期4卷 208-218页

作者： Jie Arro Jose Cuenca yingzhen yang Zhenchang Liang Peter Cousins Gan-Yuan Zhong USDA-Agricultural Research Service Grape Genetics Research UnitGeneva14456NYUSA Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resource Institute of Botanythe Chinese Academy of SciencesBeijing100093People’s Republic of China E&J Gallo Winery Modesto95353CAUSA

The shoot structure of cultivated grapevine Vitis vinifera *** exhibits a three-node modular repetitive pattern,two sequential leaf-opposed tendrils followed by a tendril-free *** this study,we investigated the molecular basis of this pattern by characterizing differentially expressed genes in 10 bulk samples of young tendril tissue from two grapevine populations showing segregation of mutant or wild-type shoot/tendril *** population was the selfed progeny and the other one,an outcrossed progeny of a Vitis hybrid,‘Roger’s Red’.We analyzed 13375 expressed genes and carried out in-depth analyses of 324 of them,which were differentially expressed with a minimum of 1.5-fold changes between the mutant and wild-type bulk samples in both selfed and cross populations.A significant portion of these genes were direct cis-binding targets of 14 transcription factor families that were themselves differentially ***-based dependency analysis further revealed that most of the significantly rewired connections among the 10 most connected hub genes involved at least one transcription ***3 and MYB12,which were known important for plant-form development,were among these transcription *** importantly,TCP3 and MYB12 were found in this study to be involved in regulating the lignin gene PRX52,which is important to plant-form development.A further support evidence for the roles of TCP3-MYB12-PRX52 in contributing to tendril phyllotaxy was the findings of two other lignin-related genes uniquely expressed in the mutant phyllotaxy background.

关键词： Vitis analysis pattern

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Thermal-responsive genetic and epigenetic regulation of DAM cluster controlling dormancy and chilling requirement in peach floral buds

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Horticulture Research 2020年第1期7卷 1276-1289页

作者： Hong Zhu Pao-yang Chen Silin Zhong Chris Dardick Ann Callahan Yong-Qiang An Steve van Knocker yingzhen yang Gan-Yuan Zhong Albert Abbott Zongrang Liu USDA-ARS Appalachian Fruit Research StationKearneysvilleWV 25430USA Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China Institute of Plant and Microbial Biology Academia SinicaTaipei 11529Taiwan The State Key Laboratory of Agrobiotechnology School of Life ScienceThe Chinese University of Hong KongHong KongHong Kong USDA-ARS Plant Genetics Research UnitDonald Danforth Plant Science CenterSt LouisMO 63132USA Department of Horticulture Michigan State UniversityEast LansingMI 48834USA USDAARS Grape Genetic Research UnitGenevaNY 14456USA Forest Health Research and Education Center University of KentuckyLexingtonKY 40546USA

The Dormancy-associated MADS-box(DAM)gene cluster in peach serves as a key regulatory hub on which the seasonal temperatures act and orchestrate dormancy onset and exit,chilling response and floral bud developmental ***,how different temperature regimes interact with and regulate the six linked DAM genes remains ***,we demonstrate that chilling downregulates DAM1 and DAM3–6 in dormant floral buds with distinct patterns and identify DAM4 as the most abundantly expressed *** reveal multiple epigenetic events,with tri-methyl histone H3 lysine 27(H3K27me3)induced by chilling specifically in DAM1 and DAM5,a 21-nt sRNA in DAM3 and a ncRNA induced in *** induction is inversely correlated with downregulation of their cognate *** also show that the six DAMs were hypermethylated,associating with the production of 24-nt ***,the chilling-responsive dynamic of the different epigenetic elements and their interactions likely define distinct expression abundance and downregulation pattern of each *** further show that the expression of the five DAMs remains steadily unchanged or continuously downregulated at the ensuing warm temperature after chilling,and this state of regulation correlates with robust increase of sRNA expression,H3K27me3 and CHH methylation,which is particularly pronounced in *** robust increase of repressive epigenetic marks may irreversibly reinforce the chillingimposed repression of DAMs to ensure flower-developmental programming free from any residual DAM *** together,we reveal novel information about genetic and epigenetic regulation of the DAM cluster in peach,which will be of fundamental significance in understanding of the regulatory mechanisms underlying chilling requirement and dormancy release,and of practical application for improvement of plasticity of flower time and bud break in fruit trees to adapt changing climates.

关键词： epigenetic cluster chilling

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A key‘foxy’aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape‘Concord’

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Horticulture Research 2020年第1期7卷 2028-2039页

作者： yingzhen yang JoséCuenca Nian Wang Zhenchang Liang Honghe Sun Benjamin Gutierrez Xiaojun Xi Jie Arro YiWang Peige Fan Jason Londo Peter Cousins Shaohua Li Zhangjun Fei Gan-Yuan Zhong US Department of Agriculture-Agricultural Research Service Grape Genetics Research UnitGenevaNYUSA Beijing Key Laboratory of Grape Sciences and Enology Laboratory of Plant ResourcesInstitute of BotanyChinese Academy of SciencesBeijingChina Boyce Thompson Institute for Plant Research Cornell UniversityIthacaNYUSA US Department of Agriculture-Agricultural Research Service Plant Genetic Resources UnitGenevaNYUSA Forestry and Pomology Research Institute Shanghai Academy of Agricultural SciencesShanghaiChina University of Chinese Academy of Sciences BeijingChina E.&J.Gallo Winery ModestoCAUSA US Department of Agriculture–Agricultural Research Service Robert W.Holley Center for Agriculture and HealthIthacaNYUSA Present address:Centro de Citricultura y Producción Vegetal.Instituto Valenciano de Investigaciones Agrarias MoncadaValenciaSpain Present address:College of Horticulture and Forestry Huazhong Agricultural UniversityWuhanHubeiChina

‘Concord’,the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L.,possesses a special‘foxy’aroma predominantly resulted from the accumulation of methyl anthranilate(MA)in *** aroma,however,is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species *** we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key‘foxy’aroma gene,anthraniloyl-CoA:methanol acyltransferase(AMAT),that is responsible for MA *** found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in‘Concord’and other ***-derived *** promoter variants,all with direct and inverted repeats,were further confirmed in more than 1,300 Vitis ***,functional impact of these indels was validated in transgenic *** on the promoter regulation,large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some *** *** of the AMAT genetic regulation advances our understanding of the‘foxy’aroma trait and makes it genetically trackable and amenable in grapevine breeding.

关键词： insertion aroma grape

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EXPRESSION OF PERFORIN AND FAS LIGAND IN INFILTRATING CELLS OF MURINE MYOCARDIUM INFECTED WITH COXSACKIEVIRUS B3

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Chinese Medical Sciences Journal 1998年第3期13卷 161-165页

作者：熊丁丁杨英珍宿燕岗陈灏珠

Purpose. To clarify the role of perforin-and Fas ligand (L)-mediated cytotoxicity pathogenesis of viral myocarditis. Materials and methods. Forty balb/c mice were randomly divided into experimental group (n = 20) and control group (n = 20), and inoculated intraperitoneally with coxsackievirus B3(CVB3) and Eagle’s solu- tion without CVB3, respectively. The mice were sacrificed and their hearts were removed at day 7 post-in- oculation. Expression of perform and FasL were detected with immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization. Results. (1)Perform-and FasL-positiye cells were demonstrated in experimental murine hearts by im- munohistochemistry, however, no cells were discovered in control murine hearts; (2) The examination of RT-PCR showed the positive ratios of perform and FasL mRNA in myocardium were significantly higher in experimental group (100% and 100 % ) than that in control group (20% and 30 %, P<0.05); (3)Positive signals of perform and FasL mRNA were found in myocardium of all the experimental mice by in situ hybridization, but nothing was detected in control group. Conclusion. Perform and FasL can be expressed in infiltrating cells in murine myocardium with acute myocarditis caused by CVB3, suggesting perform and FasL might play an important role in pathogenesis of viral myocarditis.

关键词： viral myocarditis perform Fas ligand

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数据驱动的大规模森林场景真实感动画

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计算机辅助设计与图形学学报 2008年第8期20卷 1015-1022页

作者：宋成芳于洋杨颖振何发智朱庆彭群生武汉大学计算机学院武汉430079 浙江大学CAD＆CG国家重点实验室杭州310058 武汉大学测绘遥感信息工程国家重点实验室武汉430079

提出一种基于数据驱动的大规模森林场景真实感动画的实现方案.该方案预先对单株样本树在风力作用下的运动作高精度的动力学计算,得到高分辨率、精确的树骨架形变序列;然后采用分簇主元分析方法对形变序列数据进行压缩与简化,构建单株树... 详细信息

提出一种基于数据驱动的大规模森林场景真实感动画的实现方案.该方案预先对单株样本树在风力作用下的运动作高精度的动力学计算,得到高分辨率、精确的树骨架形变序列;然后采用分簇主元分析方法对形变序列数据进行压缩与简化,构建单株树各枝干运动的细节层次;最后基于多株样本树多个细节层次的运动数据合成大规模森林的群体运动.实验结果表明:该方案实现了力学形变数据的高效重用,在森林动画合成效率和真实感上具有一定的优势,能满足对规模和画质要求较高的应用,如影视动画与媒体广告等的需求.

关键词：森林真实感动画数据驱动多层次运动细节

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病毒性心脏病诊治新进展

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医师进修杂志 2001年第5期24卷 9-11页

作者：杨英珍陈瑞珍复旦大学医学院附属中山医院、上海市心血管病研究所、卫生部病毒性心脏病重点实验室，上海200032

病毒性心肌炎(VMC)及扩张型心肌病(简称扩心病，DCM)是心血管系统常见病、多发病。病毒性心肌炎的诊断无国际统一标准，其发病机理尚未完全明了，至今国内外对二者均无特效的药物治疗手段。少数病毒性心肌炎可演变为扩张型心肌病，后者... 详细信息

病毒性心肌炎(VMC)及扩张型心肌病(简称扩心病，DCM)是心血管系统常见病、多发病。病毒性心肌炎的诊断无国际统一标准，其发病机理尚未完全明了，至今国内外对二者均无特效的药物治疗手段。少数病毒性心肌炎可演变为扩张型心肌病，后者病死率高，以往5 a生存率约50%［1］，最近的研究发现，扩张型心肌病的发病日趋年轻化，严重影响青少年健康及社会经济的发展。虽然对扩心病的治疗如心脏移植、心肌减容术在国外已经广泛开展［2］，国内也在起步，但前者费用昂贵，后者远期疗效尚未能完全肯定。ACEI及β受体阻滞剂药物对改善扩心病症状有一定疗效［3，4］。上海市心血管病研究所通过大量基础研究，协同全国十二家综合医院，经过多方面的实验及临床研究，提出了目前药物治疗病毒性心肌炎及扩张型心肌病的较佳方案。并联同全国心血管病学会，再次修订了急性病毒性心肌炎的诊断参考标准。

关键词：病毒性心肌炎诊断治疗发病机理

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COMPARATIVE EFFECTS OF LOSARTAN AND CAPTOPRIL ON VENTRICULAR REMODELING AND FUNCTION AFTER MYOCARDIAL INFARCTION IN THE RAT

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Chinese Medical Sciences Journal 1998年第1期13卷 32-36页

作者：张高星沈学东浦寿月杨英珍潘文明陈灏珠 Shanghai Institute of Cardiovascular Diseases Zhongshan Hospital Shanghai Medical University Shanghai 200032.

Objective. To determine the effects of losartan and captopril treatment on ventricular remodeling and function after myocardial infarction in rats. Methods. Thirty-two rats with MI induced by coronary ligation after seven days were divided into four groups randomly and treated with captopril(2 g. liter-1, group A), losartan(10 mg. kg-1. d-1, group B), losartan(30 mg. kg-1. d-1,group C) and placebo (no drug, group D) for six weeks, respectively. Shamoperated rats(group E)served as controls. Echocardiography was performed at 1 and 7 weeks after MI, re- spectively. Results. Compared with the results before treatment,both LV end-diastolic internal diameter and volume decreased significantly and the thickened Posterior wall was reversed in group A, B and C; the peak early filling velocity decreased whereas the peak velocity was increased in these three groups. There are no significant difference among the three treated groups. However,LV end-diastolic internal diameter and the E/A were still increased,whereas the thickness of anterior wall and the peak velocity of LV outflow were decreased in group A,B,and C after treatment comparing with group E. Conclusion. Both angiotensin converting enzyme inhibitor and angiotensin II receptor antagonist can prevent the ventricular remodeling and improve the ventricular function.

关键词： myocardial infarction ventricular remodelin angiotensing converting enzyme inhibitor

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植物运动的层次式简化技术

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中国图象图形学报 2011年第8期16卷 1526-1531页

作者：宋成芳杨颖振彭群生武汉大学计算机学院武汉430072 浙江大学CAD&CG国家重点实验室杭州310027

利用力学预计算方法生成植物运动的真实感动画需要对形变数据作有效简化和压缩。针对植物及其运动天然具有层级性的特点,提出一种基于视觉感知层次的动态几何简化算法。依据植物的树型层级结构,将各级"子树"的运动作高低频分解;"低频运... 详细信息

利用力学预计算方法生成植物运动的真实感动画需要对形变数据作有效简化和压缩。针对植物及其运动天然具有层级性的特点,提出一种基于视觉感知层次的动态几何简化算法。依据植物的树型层级结构,将各级"子树"的运动作高低频分解;"低频运动"表征"子树"的主体运动,"高频运动"即为运动细节。按"广度优先"遍历植物层级结构,在某一"深度"(层级)上将所有"子树"的"低频运动"合成即获得原始运动在此层次的逼近。遍历深度递增将构成植物运动的渐进逼近;递减则为植物运动的逐步抽象化,这实质上实现了植物运动的细节层次控制。实验表明该算法可实现植物运动的高效简化和压缩,利于生成大规模植物场景的真实感动画。

关键词：植物模拟计算机动画动态几何简化运动压缩

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定量PCR比较病毒性心肌炎血和心肌肠道病毒RNA含量及其与心肌病变的关系

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上海医学 1999年第7期22卷 399-401页

作者：封启明杨英珍陈灏珠上海医科大学中山医院上海市心血管病研究所卫生部病毒性心脏病重点实验室

目的研究小鼠柯萨奇病毒性心肌炎模型血、心肌柯萨奇病毒ＲＮＡ含量的关系；外周血中肠道病毒ＲＮＡ含量与心肌病理积分的关系。方法利用定量ＰＣＲ方法检测病毒性心肌炎小鼠模型急性期外周血、心肌肠道病毒ＲＮＡ含量。结果ＣＶＢ３感... 详细信息

目的研究小鼠柯萨奇病毒性心肌炎模型血、心肌柯萨奇病毒ＲＮＡ含量的关系；外周血中肠道病毒ＲＮＡ含量与心肌病理积分的关系。方法利用定量ＰＣＲ方法检测病毒性心肌炎小鼠模型急性期外周血、心肌肠道病毒ＲＮＡ含量。结果ＣＶＢ３感染所致心肌炎小鼠外周血、心肌中肠道病毒ＲＮＡ含量显著正相关（ｒ＝０．９４，Ｐ＜０．０１）。外周血及心肌中肠道病毒ＲＮＡ含量与心肌病理积分显著正相关（ｒ＝０．８０，Ｐ＜０．０１，ｒ＝０．８５，Ｐ＜０．０１）。结论病毒性心肌炎急性期外周血中肠道病毒ＲＮＡ含量可以反映心肌中的病毒ＲＮＡ含量水平，且两者与心肌病理损害均有一致性改变。

关键词：定量PCR 柯萨奇B3病毒病毒性心肌炎 RNA

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急性病毒性心肌炎的药物治疗观察

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中华心血管病杂志 1999年第6期27卷 413-415页

作者： Collaborative Group of “Key National Medical Projects for the Ninth Five Year Plan Research” (Correspondence: yang yingzhen, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital,Shanghai Medical University,Shanghai 200032) 国家"九.五"科技攻关课题协作组

目的　全国十二家大型医院协作观察中西医结合治疗急性病毒性心肌炎疗效。方法　对 10 2 8例临床诊断为急性病毒性心肌炎患者随机各分两组 ,治疗组 60 2例 ,用中西医结合 (黄芪、牛磺酸、泛葵利酮、抗心律失常药等)治疗 ;对照组 4 2 6... 详细信息

目的　全国十二家大型医院协作观察中西医结合治疗急性病毒性心肌炎疗效。方法　对 10 2 8例临床诊断为急性病毒性心肌炎患者随机各分两组 ,治疗组 60 2例 ,用中西医结合 (黄芪、牛磺酸、泛葵利酮、抗心律失常药等)治疗 ;对照组 4 2 6例 ,用常规 (极化液、抗心律失常药等 )治疗。结果　中西医结合治疗组临床症状改善、外周血肠道病毒阴转、心电图ST T改变及房室传导阻滞、阵发性心房颤动、窦房传导阻滞等恢复均优于对照组(P <0 .0 1、0 .0 5 ) ;对早搏及心功能改善两组间无统计学差异(P <0 .0 5 )。结论　在目前对急性病毒性心肌炎无特效药物治疗的情况下 ,采用中西医结合治疗可作为一种治疗手段。

关键词：心肌炎病毒性心肌炎药物疗法

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