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RT-qPCR normalization of reference genes in different lifehistory stages of Gracilaria vermiculophylla(Rhodophyta)

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Journal of Oceanology and Limnology 2023年第5期41卷 1910-1917页

作者： yingyue zhang Jinxin YANG Ze YANG Cong QI Di XU Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China

The quantitative realtime polymerase chain reaction(RT-qPCR)is a powerful and sensitive method to measure expression of targeted gene but it highly relies on the use of suitable reference genes for data *** evaluated the expressions of 8 housekeeping genes:18S ribosomal rDNA(18S rDNA),28S ribosomal r DNA(28S rDNA),rubisco large subunit(rbc-L),β-actin(ACT),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),elongation factor 1(EF1),β-tubulin(Tub B),and P-phycoerythrin B(PEB),to select the suitable reference genes for different life-history stages(tetrasporophyte,carposporophyte,and male/female gametophyte)of Gracilaria vermiculophylla by absolute quantitative *** geNorm and BestKeeper were used to verify the results acquired from copy number *** show that the expression of identified reference genes varied in comparing groups composed of different type of life *** is suggested that 18S rDNA and TubB could be used for highly complex samples composed of mixed ploidy and phases.18S rDNA and 28S rDNA were also preferred for using among the matured isomorphic *** for samples with different maturities,TubB and ACT were recommended for tetrasporophytes and gametophytes respectively.

关键词： reference gene Gracilaria vermiculophylla life-history stage quantitative real-time polymerase chain reaction(RT-qPCR) red algae

Transcriptome Analysis During Tetrasporogenesis of Gracilariopsis lemaneiformis and Preliminary Study of the Expressions of Its Meiotic Genes

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Journal of Ocean University of China 2023年第2期22卷 541-554页

作者： ZHU Weifang YANG Ze XU Di YANG Jinxin zhang yingyue PAN Xiaohui The Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China

The transcriptomes of three different parts of the fertile tetrasporophyte of Gracilariopsis lemaneiformis,including tip(T),middle(M),and subjacent(S)parts,with a gradual tetrasporangium maturity were analyzed and compared to identify the genes involved in the process of *** number of differentially expressed genes(DEGs)for the Gple-S versus Gple-T comparison was 10296,and the numbers of DEGs for the Gple-S versus Gple-M and Gple-T versus Gple-M comparisons were 7435 and 1337,*** Ontology and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed,and the results showed the enrichment of 132 KEGG pathways(corrected P<0.05).A total of 58 DEGs related to meiosis were screened and blasted against 18 meiosis-related genes(dmc1,mlh1,mnd1,msh4,msh2,msh6,mre11,pds5,pms1,rad21,rad50,rad51,smc1,smc2,smc4,smc5,smc6,and spo11),including four meiosis-specific *** transcriptome comparison indicated that in the T part,the meiosis,ribosome,and RNA transport-related genes were mostly up-regulated compared with those in the other two *** the M part,the genes related to ribosomes and the endoplasmic reticulum were also up-regulated compared with those in the lower ***,in the S part,the genes associated with photosynthesis were mostly up-regulated,which might be helpful to the recovery from spore formation and release.

关键词： Gracilariopsis lemaneiformis transcriptome tetrasporogenesis meiosis related genes

Whole genome sequencing exploitation analysis of dibutyl phthalate by strain Stenotrophomonas acidaminiphila BDBP 071

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Food Bioscience 2023年第1期51卷 168-178页

作者： yingyue zhang Jie Tang Min Wu Xuerui Zhou Su Wang Hong Ye Wenliang Xiang Qing zhang Ting Cai School of Food and Bioengineering Xihua UniversityChengdu610039SichuanPeople’s Republic of China

Dibutyl phthalate(DBP)is widely used as a plasticizer in plastic food packaging and has attracted extensive attention due to its residual hazards and ability to *** degradation is a very effective way to remove DBP from a polluted *** this study,Stenotrophomonas acidaminiphila BDBP 071,a strain that efficiently degraded DBP was isolated from tomato rhizosphere *** obtain a comprehensive understanding of the degradation mechanism of DBP by *** strain BDBP 071,whole genome sequencing of this strain was *** results showed that the genome size of BDBP 071 was 3.87 Mb,the G+C content was 69.43%,and the number of predicted coding sequences was *** on whole genome sequencing,the metabolic pathway related to DBP biotransformation was obtained,and key genes were subsequently verified by a real-time quantitative polymerase chain reaction to infer the degradation pathway of *** was preliminarily predicted that the relative expression of monoester hydrolase of EstB3 is increased in this *** study provides a scientific basis for applying *** BDBP 071 in environmental pollution bioremediation,as well as a rich resource for DBP biodegradation genes.

关键词： Dibutyl phthalate Stenotrophomonas acidaminiphila Whole genome sequencing RT-qPCR

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基于场域理论的亲子阅读服务需求研究

情报科学 2023年第7期41卷 115-124页

作者：周昕张赢月吉林大学商学与管理学院吉林长春130012

【目的/意义】针对当前亲子阅读服务现状,研究亲子阅读服务需求,并结合现实提出相应的建议,为亲子阅读服务提供参考。【方法/过程】以场域理论为基础,通过线上访谈,整理资料得到亲子阅读服务需求场域,并通过发放调查问卷,对回收的问卷... 详细信息

【目的/意义】针对当前亲子阅读服务现状,研究亲子阅读服务需求,并结合现实提出相应的建议,为亲子阅读服务提供参考。【方法/过程】以场域理论为基础,通过线上访谈,整理资料得到亲子阅读服务需求场域,并通过发放调查问卷,对回收的问卷进行数据整理,通过spss检验信效度并进行探索因子分析,计算李克特量表均值,最终验证分析合理,构建亲子阅读服务需求场域模型。【结果/结论】亲子阅读服务需求场域模型的核心为亲子阅读辅助服务需求,其主要包括两个主要服务需求场,即亲子阅读服务需求资本场和亲子阅读服务需求惯习场,前者具体包括数字亲子图书资源、纸质亲子图书资源、专业指导知识;后者则包括亲子阅读服务方式、亲子阅读空间、硬件设施、亲子阅读氛围、多样化亲子阅读活动、安全保障。【创新/局限】本文利用场域理论,以亲子共同体为内部主体,通过调查分析构建了亲子阅读服务需求场域以及场域的资本和惯习。未来将进一步扩大的样本数量和范围、增加问卷的针对性,并针对服务需求进行具体完善的服务体系设计。

关键词：场域理论亲子阅读服务需求文化资本惯习亲子阅读服务需求模型

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心肌梗死患者进行抗阻运动的研究进展

解放军医学院学报 2021年第4期42卷 460-463,F0003页

作者：张瀛月马晶徐勇解放军医学院北京100853 解放军总医院第一医学中心心血管内科北京100853

心肌梗死是一种预后不佳的急性心血管疾病。抗阻运动,如提踵,是以增加肌肉力量为目的的系统、科学的运动。抗阻运动作为运动心脏康复的一部分,在心肌梗死的一级及二级预防中有重要作用,可改善心脏功能,减轻肌肉力量减退,提高生活质量,... 详细信息

心肌梗死是一种预后不佳的急性心血管疾病。抗阻运动,如提踵,是以增加肌肉力量为目的的系统、科学的运动。抗阻运动作为运动心脏康复的一部分,在心肌梗死的一级及二级预防中有重要作用,可改善心脏功能,减轻肌肉力量减退,提高生活质量,使患者更好地回归工作和生活。本文对心肌梗死患者进行抗阻运动可能获得的益处和机制及其实际应用中的问题进行综述,旨在促进抗阻运动在心肌梗死患者中的应用。

关键词：抗阻运动心肌梗死心脏康复心力衰竭康复治疗

Simultaneous measurements of refractive index and temperature based on a no-core fiber coated with Ag and PDMS films

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Chinese Physics B 2023年第5期32卷 471-477页

作者：李宇昕陈海良张赢月陈强武彪樊晓亚刘英超马明建 State Key Laboratory of Metastable Materials Science&Technology and Key Laboratory for Microstructural Material Physics of Hebei Province School of ScienceYanshan UniversityQinhuangdao 066004China College of Artificial Intelligence North China University of Science and TechnologyTangshan 063210China

A compact surface plasmon resonance(SPR) fiber optic sensor, being utilized to simultaneously measure refractive index(RI) and temperature, is proposed and experimentally demonstrated in this paper. One part of a no-core fiber(NCF)was coated with a silver(Ag) film, and the other part was coated with a silver/polydimethylsiloxane(Ag/PDMS) composite film to stimulate the SPR effect. Due to the two heterogeneous films, two dips appeared in the transmission spectrum and were used to achieve the dual-parameter measurements. The experimental results showed that the RI sensitivity reached 2121.43 nm/RIU and 0 nm/RIU, while the temperature sensitivity reached-0.32 nm/℃ and-2.21 nm/℃ for the two dips,respectively. Based on the obtained transfer matrix, the measurements of RI and temperature could be demodulated. This designed sensor showed the merits of simple structure, easy to implement, and high sensitivity, demonstrating application prospects in dual-parameter monitoring.

关键词： no-core fiber surface plasmon resonance temperature sensor

发展型p-Laplace方程边界最优控制的存在性

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吉林大学学报（理学版） 2023年第1期61卷 41-45页

作者：张瀛月杜润梅长春工业大学数学与统计学院长春130012

通过对成本泛函的极小化序列取极限给出发展型p-Laplace方程初边值问题最优控制函数的存在性.先用能量估计方法研究该问题解的存在唯一性,再用紧性估计和紧嵌入定理分析成本泛函极小化序列的收敛性,最后证明最优控制函数的存在性.

关键词：发展型p-Laplace方程最优控制边界控制

辣椒主要生产环节机械化技术在宝坻的示范应用

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农业技术与装备 2021年第7期 56-57页

作者：张滢月天津市宝坻区农业发展服务中心天津301800

目前,宝坻区辣椒种植仍然以人工作业为主,存在种植方式落后、劳动强度大、生产成本高、作业效率低等问题,使农民的收入得不到提高,严重制约了辣椒种植业的发展。在辣椒种植中的主要生产环节实现机械化可以很好地解决上述问题。文章介绍... 详细信息

目前,宝坻区辣椒种植仍然以人工作业为主,存在种植方式落后、劳动强度大、生产成本高、作业效率低等问题,使农民的收入得不到提高,严重制约了辣椒种植业的发展。在辣椒种植中的主要生产环节实现机械化可以很好地解决上述问题。文章介绍了辣椒主要生产环节机械化技术在宝坻的示范应用。

关键词：辣椒主要生产环节机械化

内源性n-3多不饱和脂肪酸对下丘脑神经干细胞来源外泌体miRNA的调节

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食品科学 2022年第13期43卷 140-145页

作者：路宗博张盈月葛科立张金玉薛美兰葛银林青岛大学基础医学院山东青岛266073

目的:通过培养fat-1转基因小鼠下丘脑神经干细胞获取外泌体并提取总RNA进行高通量测序,研究内源性n-3多不饱和脂肪酸(n-3 polyunsaturated fatty acids,n-3 PUFAs)对丘脑神经干细胞来源外泌体miRNA的调节作用,探究n-3 PUFAs抑制下丘脑... 详细信息

目的:通过培养fat-1转基因小鼠下丘脑神经干细胞获取外泌体并提取总RNA进行高通量测序,研究内源性n-3多不饱和脂肪酸(n-3 polyunsaturated fatty acids,n-3 PUFAs)对丘脑神经干细胞来源外泌体miRNA的调节作用,探究n-3 PUFAs抑制下丘脑炎症及肥胖的作用机制。方法:分别培养fat-1转基因小鼠与野生型C57BL/6小鼠下丘脑神经干细胞,从细胞培养上清液中收集外泌体并提取总RNA,经高通量测序后利用生物信息学技术对差异表达的miRNA进行分析及靶基因预测,对靶基因进行基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析后,利用实时荧光定量聚合酶链式反应(quantitative real-time polymerase chain reaction,qPCR)进行相对表达量的测定。结果:与野生型C57BL/6小鼠相比,fat-1转基因小鼠下丘脑神经干细胞来源的外泌体miRNA具有特异表达谱,且具有显著差异表达的miRNA靶基因处于下丘脑炎症、脂肪酸代谢等通路中关键位置。经qPCR验证,差异表达的miRNA关键靶基因表达均受到调控。结论:内源性n-3 PUFAs水平的增加可以调节下丘脑神经干细胞外泌体中miRNA的表达,进而调控炎症反应与脂质代谢相关基因的表达,从而发挥抑制下丘脑炎症、抵抗肥胖的作用。

关键词：内源性n-3多不饱和脂肪酸外泌体 miRNA 下丘脑炎症脂质代谢