检索结果-南通市图书馆

Desialylated Platelet Clearance in the Liver is a Novel Mechanism of Systemic Immunosuppression

Research 2024年第3期2023卷 35-50页

作者： June Li Danielle Karakas Feng Xue yingyu chen Guangheng Zhu Yeni H.Yucel Sonya A.MacParland Haibo Zhang John W.Semple John Freedman Qizhen Shi Heyu Ni Department of Laboratory Medicine and Pathobiology University of TorontoTorontoONCanada Toronto Platelet Immunobiology Group TorontoONCanada Keenan Research Centre for Biomedical Science of St.Michael's Hospital TorontoONCanada Canadian Blood Services Centre for Innovation TorontoONCanada Departments of Pediatrics Medical College of WisconsinMilwaukeeWIUSA Blood Research Institute Versiti WisconsinMilwaukeeWIUSA Departments of Ophthalmology and Vision Sciences Medicine University of TorontoTorontoONCanada Faculty of Engineering and Architectural Science Ryerson UniversityTorontoONCanada Multi-Organ Transplant Program Toronto General Hospital Research InstituteTorontoONCanada Department of Immunology University of TorontoTorontoONCanada Critical Care Medicine Department ofAnesthesiology and PainUniversity of TorontoTorontoONCanada Department of Physiology University of TorontoTorontoONCanada Department of Pharmacology University of TorontoTorontoONCanada. Division of Hematology and Transfusion Medicine Lund UniversityLundSweden clinical Immunology and Transfusion Medicine Ofice of Medical ServicesRegion SkaneLundSweden Department of Medicine University of TorontoTorontoONCanada Children's Research Institute Children's WisconsinWauwatosaWIUSA Midwest Athletes Against Childhood Cancer Fund Research Center MilwaukeeWIUSA.

Platelets are small, versatile blood cells that are critical for hemostasis/thrombosis. Local platelet accumulation is a known contributor to proinflammation in various disease states. However, the anti-inflammatory/immunosuppressive potential of platelets has been poorly explored. Here, we uncovered, unexpectedly, desialylated platelets (dPLTs) down-regulated immune responses against both platelet-associated and -independent antigen challenges. Utilizing multispectral photoacoustic tomography, we tracked dPLT trafficking to gut vasculature and an exclusive Kupffer cell-mediated dPLT clearance in the liver, a process that we identified to be synergistically dependent on platelet glycoprotein Ibα and hepatic Ashwell–Morell receptor. Mechanistically, Kupffer cell clearance of dPLT potentiated a systemic immunosuppressive state with increased anti-inflammatory cytokines and circulating CD4+ regulatory T cells, abolishable by Kupffer cell depletion. Last, in a clinically relevant model of hemophilia A, presensitization with dPLT attenuated anti-factor VIII antibody production after factor VIII ( infusion. As platelet desialylation commonly occurs in daily-aged and activated platelets, these findings open new avenues toward understanding immune homeostasis and potentiate the therapeutic potential of dPLT and engineered dPLT transfusions in controlling autoimmune and alloimmune diseases.

关键词： Platelet transfusion homeostasis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evaluation and application of a milk antibody ELISA for assessing the prevalence and incidence of bovine tuberculosis in dairy herds in Hubei Province,China

引用

Animal Diseases 2023年第2期3卷 143-151页

作者： yingyu chen Shuang cheng Yu Wang Guiqiang Wang Xijuan Wu Ian D.Robertson Aizhen Guo State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityHubei ProvinceWuhan 430070China College of Veterinary Medicine Huazhong Agricultural UniversityHubei ProvinceWuhan 430070China National Animal Tuberculosis Para-Reference Laboratory(Wuhan) Huazhong Agricultural UniversityHubei ProvinceWuhan 430070China Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology Huazhong Agricultural UniversityHubei ProvinceWuhan 430070China Hubei Livestock and Poultry Centre Wuhan 430070China School of Veterinary Medicine Murdoch UniversityMurdoch 6150Australia

Bovine tuberculosis(bTB)is a chronic zoonotic disease that is endemic in *** in-vitro tests for bTB are mainly based on blood *** of samples results in some stress to the sampled cattle and associated economic losses for the herd *** study was designed to investigate the relationship between milk and serum antibody tests for bTB in dairy cows using 85 cows with milk and corresponding blood *** 4,395 milk samples were used to assesse the apparent(test)prevalence and incidence of bTB using the milk antibody *** association between levels of bTB milk antibody and milk quality was also *** and serum antibody tests showed a good correlation with a 87.5%(95%CI:61.7%,98.4)positive agreement and 98.7%(95%CI:95.4,99.8)negative *** animal level lactoprevalence ranged from 0.3%(95%CI:0,1.2)to 33.3%(95%CI:26.6,40.6)in different farms and the incidence rate ranged from 0 head/cow-month(95%CI:0,0.02)to 0.04 head/cow-month(95%CI:0.02,0.07).Twenty percent of sampled farms met the criteria for bTB control in *** prevalence on large-scale farms was lower(p<0.001)than on small *** bTB milk antibody levels had a negative correlation with milk yield and a positive correlation with somatic cell count(SCC),milk protein percentage(MPP)and percentage of total solids(TS).According to this research,milk ELISA could be used as a supplement of blood samples to assist in the surveillance for bTB and for alerting control and eradication of bTB.

关键词： Bovine Tuberculosis Milk antibody Prevalence Incidence rate Milk quality

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

PDCD5 regulates iNKT cell terminal maturation and iNKT1 fate decision

引用

Cellular & Molecular Immunology 2019年第9期16卷 746-756页

作者： Ke Wang Xinwei Zhang Yifan Wang Gaowen Jin Mingyang Li Shusong Zhang Jie Hao Rong Jin Xiaojun Huang Hounan Wu Jun Zhang yingyu chen Qing Ge Key Laboratory of Medical Immunology Ministry of Health.Department of ImmunologySchool of Basic Medical SciencesPeking University Health Science Center38 Xue Yuan RoadBeijingChina Peking University Institute of Hematology People’s HospitalBeijingChina Peking University Medical and Health Analytical Center Peking University Health Science CenterBeijingChina

Invariant natural killer T1(iNKT1)cells are characterized by the preferential expression of T-box transcription factor T-bet(encoded by Tbx21)and the production of cytokine IFN-γ,but the relationship between the developmental process and iNKT1 lineage diversification in the thymus remains *** report in the present study a crucial role of programmed cell death 5(PDCD5)in iNKT cell terminal maturation and iNKT1 fate *** with T cell-specific deletion of PDCD5 had decreased numbers of thymic and peripheral iNKT cells with a predominantly immature phenotype and defects in response toα-*** of PDCD5 also selectively abolished the iNKT1 lineage by reducing T-bet expression in iNKT cells at an early thymic developmental stage(before CD44 upregulation).We further demonstrated that TOX2,one of the high mobility group proteins that was highly expressed in iNKT cells at stage 1 and could be stabilized by PDCD5,promoted the permissive histone H3K4me3 modification in the promoter region of *** data indicate a pivotal and unique role of PDCD5/TOX2 in iNKT1 lineage *** also suggest that the fate of iNKT1 may be programmed at the developmental stage of iNKT cells in the thymus.

关键词： determination. PDCD5 NKT

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein kinase C iota(PRKCI) negatively regulates autophagy via AKT-mTOR signaling in U2OS osteosarcoma cells

Protein kinase C iota(PRKCI) negatively regulates autophagy ...

引用

第十届全国免疫学学术大会

作者： Liujing Qu yingyu chen Key Laboratory of Medical Immunology Ministry of Health Peking University Health Science Center

Objective:Protein kinase C(PRKC)is a family of serine/threonine kinases,and these PRKC isozymes have crucial roles in diverse cellular signaling processes including cellular proliferation,differentiation,apoptosis and *** iota(PRKCI),one member of the atypical PRKC isoforms,has been shown to be a human oncogene compared with some other diverse PKC ***:Autophagy was confirmed by microtubule-associated protein light chain 3(LC3)conversion and measuring the level of p62/*** fusion of autophagosome and lysosome was viewed by confocal and immumofluorescence ***-and chemical-induced autophagy was examined by western blot and confocal *** level of PRKCI and p62 were detected by ***:Overexpressed PRKCI also contributes to the poor prognosis and aggressive tumor phenotypes in tumor *** the present study,we demonstrated the role of PRKCI on cell *** PRKCI overexpressed U2OS cells,autophagosome formation and substrate degradation were inhibited either in normal or stress *** effects were evidenced by decreased LC3B-II levels,increased endogenous SQSTM1/p62 accumulation and exogenous Poly *** the PRKCI knockout U2OS cells,the opposite effect was *** addition,we identified two PRKCI mutants,PRKCIL485M and PRKCIp560R,which localize the STKc PKC *** can induce cell autophagy which is similar to *** may be dominant negative mutants of ***,we investigate the cell signaling of PRKCI mediated *** is noted that PRKCI regulates autophagy through AKT-m TOR *** significantly,PRKCI expression was well correlated with autophagic capacity in human osteosarcoma tissues,in that SQSTM1/p62 degradation was decreased when PRKCI expression was increased in human osteosarcoma ***:Protein kinase C iota(PRKCI)negatively regulates autophagy via AKT-m TOR signaling in U2OS osteosarcoma *** study also provide

关键词： PRKCI autophagy mTOR osteosarcoma

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Programmed cell death 5(PDCD5) negatively regulates autoimmunity

Programmed cell death 5(PDCD5) negatively regulates autoimmu...

引用

第十届全国免疫学学术大会

作者： Ge Li Juan Xiao yingyu chen Department of Immunology Peking University School of Basic Medical Sciences Peking University Center for Human Disease Genomics Medical College Hubei University of Arts and Science

Purpose:This study aims to investigate the roles of PDCD5 in autoimmune disease and to probe into its molecular mechanism of ***:We constructed some autoimmune disease models such as autoimmune encephalomyelitis(EAE),collageninduced arthritis(CIA)and DSS-induced ulcerative colitis(UC)to detect whether increased PDCD5 protein could relieve autoimmune *** these models,PDCD5 transgenic mice were used,or rh PDCD5 were injected to mice or rats.

关键词： PDCD5 Autoimmune disease Regulatory T cell FOXP3

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

A case study investigating the efects of emergency vaccination with Brucella abortus A19 vaccine on a dairy farm undergoing an abortion outbreak in China

引用

Animal Diseases 2022年第4期2卷 246-257页

作者： Yu Wang Yan Wang Qingjie Peng Zhijie Xiang yingyu chen Guiqiang Wang Xijuan Wu Aizhen Guo Ian D.Robertson State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan 430070China College of Veterinary Medicine Huazhong Agricultural UniversityWuhanChina Hubei International Scientifc and Technological Cooperation Base of Veterinary Epidemiology Huazhong Agricultural UniversityWuhanChina China-Australia Joint Research and Training Centre for Veterinary Epidemiology Huazhong Agricultural UniversityWuhanChina Technology Centre of Bio-Agricultural Angel Yeast CoLtdYichangChina Hubei Hongshan Laboratory Huazhong Agricultural UniversityWuhanChina Hubei Dairy Herd Improvement Center WuhanChina School of Veterinary Medicine Murdoch UniversityPerthWAAustralia

Brucellosis is an important zoonosis that results in substantial economic losses to the livestock industry through abortions and reduced milk *** study investigated an abortion outbreak in a dairy herd and then explored the efects of emergency vaccination with Brucella abortus A19 vaccine on the incidence of abortion and milk yield.A full dose of vaccine(6×10^(10)—12×10^(10)colony forming units,CFU)was administered subcutaneously to calves and non-pregnant heifers,and a reduced dose(6×10^(8)—12×10^(8)CFU)to adult cows and pregnant replacement *** Bengal Test was used to screen Brucella infection status and then positive samples were tested with a *** that tested positive for both tests were considered positive to Brucella *** animal-level seroprevalence of brucellosis was 23.1%(95%CI:17.0,30.2),and the attributable fraction of abortions in seropositive animals was 89.1%(95%CI:64.3,96.7).The odds of seropositivity were signifcantly higher in cows that aborted compared to cows that calved normally(OR=21.4,95%CI:4.4,168.4).Cows in sheds A2 and C1 were 10.2(95%CI:1.4,128.0)and 17.0(95%CI:2.8,190.3)times more likely to be seropositive than cows in shed *** were not detectable in most heifers 12 months *** efectiveness of the vaccine in preventing abortions was estimated to be 56.8%(95%CI:15.8,77.8)for the entire herd,but increased to 86.7%(95%CI:4.4,98.1)when only primiparous heifers were ***,a signifcant increase in the average herd 305-day milk yield one-year after vaccination was also observed relative to that in the previous three *** is concluded that emergency vaccination of a dairy herd undergoing an abortion outbreak with the A19 vaccine efectively reduced the incidence of abortion and indirectly increased milk yield one-year after vaccination.

关键词： Brucellosis Outbreak investigation Emergency vaccination Brucella abortus A19 vaccine Abortion 305-day milk yield

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

近水面水下爆炸气泡强耦合运动特性研究

引用

中国造船 2016年第3期57卷 65-71页

作者：陈莹玉姚熊亮哈尔滨工程大学船舶工程学院哈尔滨150001

近自由面水下爆炸气泡三维动态特性非常复杂,与很多因素有关。采用ABAQUS/Explicit中的欧拉技术模拟气泡与自由面的强耦合作用。建立近水面水下爆炸气泡运动的计算模型,对近自由面流场中的膨胀、坍塌及射流的形成,自由面上水冢的产生和... 详细信息

近自由面水下爆炸气泡三维动态特性非常复杂,与很多因素有关。采用ABAQUS/Explicit中的欧拉技术模拟气泡与自由面的强耦合作用。建立近水面水下爆炸气泡运动的计算模型,对近自由面流场中的膨胀、坍塌及射流的形成,自由面上水冢的产生和撕裂等动力学行为进行分析,旨在为模拟水下爆炸气泡与自由面的强非线性耦合作用提供一种新的方法。

关键词：欧拉法水下爆炸气泡自由面

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genetic diversity,population structure and core collection analysis of Hunan tea plant germplasm through genotyping-by-sequencing

引用

Beverage Plant Research 2022年第1期2卷 36-42页

作者： Feiyi Huang Jihua Duan Yu Lei Zhen Liu Yankai Kang Yi Luo yingyu chen Youyong Li Shuoqian Liu Saijun Li Zhonghua Liu College of Horticulture Hunan Agricultural UniversityChangsha 410128China Tea Research Institute Hunan Academy of Agricultural Sciences/National Centre for Tea ImprovementHunan Branch/Hunan Tea Variety and Seedling Engineering Technology Research CenterChangsha 410125China Tea Research Institute Yunnan Academy of Agricultural SciencesMenghai 666200China

Tea is considered to be a well-known and widely consumed beverage and Hunan province is rich in tea plant *** order to better conserve and utilize Hunan tea plant resources,110 tea accessions from seven geographical origins were used to assess genetic diversity of Hunan tea plant germplasm through genotyping by sequencing(GBS)*** a result,a total of 311,044 high-quality single nucleotide polymorphism(SNP)markers were *** structure,phylogenetic relationships and principal component analysis(PCA)divided the entire accessions into three *** genetic diversity and population differentiation analysis showed that the mean observed heterozygosity(Ho)ranged from 0.16 to 0.24,while the mean polymorphic information content(PIC)ranged from 0.14 to 0.17,and mean minor allele frequency(MAF)ranged from 0.11 to *** of molecular variance(AMOVA)indicated that 81.38%of the total variance was derived from within populations,which suggested a rich genetic diversity in Hunan tea ***,a core tea germplasm set was developed,which was comprised of 22 tea plant accessions and maintained the whole genetic diversity of the entire *** work should be valuable for conservation and utilization of tea germplasm in Hunan。

关键词： Hunan germplasm analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Really interesting new gene finger protein 121(RNF121) is anovel Golgi-localized membrane protein that regulates apoptosis

Really interesting new gene finger protein 121(RNF121) is an...

引用

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： Yuanbo Zhao Dubeiqi Hong Dalong Ma yingyu chen Key Laboratory of Medical Immunology Ministry of Health Peking University Health Science Center Peking University Center for Human Disease Genomics Peking University

Really interesting new gene(RING)finger protein is a large protein family in the *** have very important functions in physiological activities and cancer *** biological functions of many RING finger proteins remain *** thisstudy,we describe the biological activity of a novel,human Golgi-localized RING fingerprotein 121(RNF121),the function of which is,thus far,*** RNF121 proteincontains five conserved TM domains and that the RING finger domain faces the *** endoplasmic reticulum-localized RNF121 in Caenorhabditis elegans,human RNF121was ***121 predominantly localized to the Golgi apparatus in ***121 mRNA is expressed in a variety of normal human ***121 knockdown inhibited cell growth and induced apoptosis,which was accompaniedby caspase-3 activation and the inactivation of poly(adenosine diphosphate-ribose)polymerase.Z-VAD-FMK,a pan-caspases inhibitor,inhibited the RNF121knockdown–*** of wild-type RNF121 decreased RNF121 knockdown-inducedapoptosis but the RING domain mutants of RNF121did not have this *** theRING-domain mutated(RNF121 C229A C259A)or truncated RNF121(RNF121 1-225)couldn’t alleviate the cell death induced by knockdown of ***121 C229A C259Aand RNF121 1-225 also showed no anti-apoptosis effect when *** indicatesthat the RING domain is required for RNF121-regulated ***,RNF121 knockdown enhanced etoposide-induced *** RNF121has anti-apoptosis effect in cancer cells,and sheds new light to the relation betweenRNF121 and *** finger proteins have been proved to play crucial roles in *** is possible that RNF121 regulates apoptosis through targeting apoptosis-related molecules for *** is also possible that RNF121 regulates apoptosisthrough regulation of Golgi-related transport *** study instigates further investigation to the exact mechanism that RNF121 regula

关键词： RING finger protein 121 RNF121 apoptosis RING domain Golgi apparatus

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Risk factors associated with Salmonella in dairy cattle farms in Henan and Hubei provinces,China

引用

Animal Diseases 2023年第4期3卷 256-264页

作者： Jie Wang Xiaojie Zhu Yuxi Zhao Yongkang Xue Zhen Zhang Lei Yan yingyu chen Ian D.Robertson Aizhen Guo Joshua W.Aleri The State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China School of Veterinary Medicine College of ScienceHealthEngineering and EducationMurdoch University90 South StreetMurdochWA 6150Australia Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology Huazhong Agricultural UniversityWuhan 430070China Henan Dairy Herd Improvement Center Zhengzhou 450046China Centre for Animal Production and Health Future Foods InstituteMurdoch University90 South StreetMurdochWA 6150Australia

A cross-sectional study was conducted to evaluate the risk factors associated with the Salmonella infection status of dairy herds in Henan and Hubei provinces,*** were assigned a Salmonella status based on the isolation of Salmonella from fecal samples obtained from adult lactating dairy *** on potential biosecurity risk factors was collected using a questionnaire template via a 15-min face-to-face interview with dairy cattle producers from May 2020 to March *** questionnaire consisted of 23 questions across two broad categories of potential biosecurity risk factors:farm and biosecurity managemental factors.A total of 48 farms were *** all(100%,48/48)of the surveyed farms,although sick animals were separated from healthy animals using fencing,there were no strict quarantine protocols for newly introduced *** species rearing was practiced in 35.4%(17/48)of the *** were removed more than once a day in 45.8%(22/48)of the farms,whereas the remainder(54.2%,26/48)only removed animal feces once a day.A total of 29.2%(14/48)of the farms were located<2 km from other livestock *** use of personal protective equipment was consistently performed on all farms,with a major-ity of the workers on most farms(81.3%,39/48)always disinfecting footwear before entering production areas.A significant association between Salmonella-positive status and the high frequency of the presence of wildlife(birds and rodents in sheds and feed storage places)was recorded(OR:11.9,95%Cl:1.7,84.1,p=0.013).Fortunately,no farms shared farm equipment with other *** study highlights the occurrence of wildlife as a risk factor for the presence of Salmonella in investigated dairy *** is a need to institute appropriate on-farm control measures for wild birds and rodents to control the potential spread of Salmonella in dairy production systems.

关键词： Cattle Management practices Risk factors Salmonella

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：