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魁蚶3个群体及杂交子代遗传多样性分析

水产科学 2024年第4期43卷 561-570页

作者：刘童王英俊吴莹莹邹琰吕芳吴海一李建民宋爱环山东省海洋科学研究院山东青岛266104 青岛市近海生态修复与安全保障重点实验室山东青岛266104 山东省智慧海洋牧场重点实验室(筹) 山东青岛266104 青岛市海洋生物种质资源挖掘与利用工程实验室山东青岛266104 日照山海天旅游度假区渔政监督管理站山东日照276800

为探明魁蚶不同群体间遗传多样性差异,以魁蚶中国群体、韩国群体和杂交子一代群体为研究对象,利用线粒体基因(16S rRNA和COⅠ)和核糖体内部转录间隔区(ITS-1)对5个魁蚶群体共150个样本进行遗传多样性分析。结果显示,魁蚶16S rRNA、COⅠ... 详细信息

为探明魁蚶不同群体间遗传多样性差异,以魁蚶中国群体、韩国群体和杂交子一代群体为研究对象,利用线粒体基因(16S rRNA和COⅠ)和核糖体内部转录间隔区(ITS-1)对5个魁蚶群体共150个样本进行遗传多样性分析。结果显示,魁蚶16S rRNA、COⅠ和ITS-1同源序列片段长度分别为605、766 bp和419 bp,其中16S rRNA和COⅠ基因片段A+T含量均高于G+C含量,ITS-1片段A+T含量均低于G+C含量。16S rRNA和COⅠ基因分别定义了4个和14个单倍型,主体单倍型所占频率分别为81.58%和61.43%,ITS-1定义了24种单倍型,主体单倍型所占频率为50.67%。单倍型邻接关系树结果显示,16S rRNA和COⅠ基因均为4个子一代群体聚为1支,即墨群体独有的单倍型为1支,而ITS-1的分支无明显群体特征。16S rRNA和COⅠ的遗传分化结果均表明,即墨群体与其他4个群体间遗传分化较大,其他4个群体间不存在分化;ITS-1的结果显示,5个群体间的遗传分化指数值均小于0.25,遗传分化不明显。试验结果表明,在进行魁蚶种内遗传进化研究时,线粒体基因的敏感性高于核糖体基因,即墨群体的遗传多样性明显高于4个子一代群体。

关键词：水生生物学遗传多样性线粒体DNA 核糖体DNA 魁蚶

A 5D simulation method on post-earthquake repair process of buildings based on BIM

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Earthquake Engineering and Engineering Vibration 2020年第3期19卷 541-560页

作者： Xu Zhen Zhang Furong Jin Wei wu yingying Qi Mingzhu Yang Yajun Beijing Key Laboratory of Urban Underground Space Engineering School of Civil and Resource EngineeringUniversity of Science and Technology BeijingBeijing 100083China

The post-earthquake repair process of buildings is one of the key components of building seismic *** the details of the repair process in advance will benefit the evaluation and decision making for the seismic resilience of *** this end,a five-dimensional(5 D)simulation method on the post-earthquake repair process of buildings is proposed based on building information modeling(BIM),by which the repair activities can be presented in the way associated with a 3 D model,time(denoted as the fourth dimension),and the related costs(denoted as the fifth dimension).First,a decision-making model of repair schemes based on the interval index of the possibility degree is created to determine the optimal post-earthquake repair schemes for a damaged ***,an algorithm based on separate flowrepetitive process is designed to automatically make the repair schedule on a BIM platform according to the determined repair ***,an algorithm of automatic mapping between the components in BIM and their repair schedules is developed,so that a 5 D simulation of a post-earthquake repair process can be implemented.A six-story office building is selected as a case study,and the details of the post-earthquake repair process(e.g.,the costs of labor,material and equipment over the repair time)are presented by the proposed 5 D simulation *** outcome of this study can be used to evaluate the seismic resilience of buildings and also to facilitate the decision making for seismically resilient buildings by providing the detailed guidance of the repair process.

关键词： BIM details repair

Shock Characteristics and Protective Design of Equipment During Spacecraft Docking Process

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Acta Mechanica Solida Sinica 2023年第6期36卷 837-850页

作者： Xu Gao Xianxian He yingying wu Yifeng Dong Jiajing Huo Ying Li Beijing Institute of Spacecraft System Engineering Beijing 100094China School of Naval Architecture Ocean and Energy Power EngineeringWuhan University of TechnologyWuhan 430064China Institute of Advanced Structure Technology Beijing Institute of TechnologyBeijing 100081China

The shock loads generated by spacecraft during docking can cause functional failure and structural damage to aerospace electronic equipment and even lead to catastrophic flight *** is currently a lack of systematic and comprehensive research on the shock environment of spacecraft electronic equipment due to the diversity and complexity of the shock *** this paper,the validity of the finite element model is verified based on the sinusoidal vibration experiment results of the spacecraft reentry *** method of shock dynamic response analysis is used to obtain the shock environment of electronic equipment under different shock *** shock response spectrum is used to describe the shock environment of aerospace electronic *** results show that the resonance frequency error between the sinusoidal vibration experiment and the model is less than 4.06%.When the docking relative speed of the reentry capsule is 2 m/s,the shock response spectrum values of one of the equipment are 30 m2/s,0.67 m/s,and 0.059 m,*** wire rope spring on the mating surface can provide vibration isolation and shock *** increase in spring damping coefficient results in a decrease in the amplitude and time of the vibration *** increase in spring stiffness reduces the input of shock load within a certain *** research results can provide guidance for the design and evaluation of shock environmental adaptability of aerospace electronic equipment.

关键词： Shock environment Shock response spectrum Spacecraft docking Protective design

Synthesis and Herbicidal Activities of Novel Uracil Derivatives Containing Pyrimidinyl Moiety

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Chemical Research in Chinese Universities 2014年第3期30卷 400-404页

作者： HE Leien wu yingying ZHANG Hanyun LIU Manyun SHI Deqing Key Laboratory of Pesticide & Chemical Biology Ministry of Education College of Chemistry Central China Normal University Wuhan 430079 P. R. China

In order to find novel protoporphyrinogen oxidase inhibitors with high efficacy, broad-spectrum activity, and safety to crops, nine title compounds（4a-4i） were designed and synthesized by introducing pyrimidine moiety into the uracil skeleton with commercially herbicide butafenacil as the lead compound. Their structures were con- firmed by 1H NMR, IR, mass spectroscopy and elemental analysis. The bioassay results indicate that most of com- pounds 4 tested exhibit good to excellent herbicidal activities against B. campestris, A. retroflexus, E. crusgalli and D. sanguinalis in pre-emergence treatment at a dose of 1.5 kg/ha（1 ha=10^4 m^2）, for example, compound 4i showed 100% inhibition against the four plants tested in pre-emergence treatment at a dose of 1.5 kg/ha. So, this type of skeleton can be used as a valuable lead compound for the further development of a pre-emergent herbicide.

关键词： Protoporphyrinogen oxidase Uracil Pyrimidine Herbicidal activity

Comparative analysis of mitochondrial genome of a deepsea crab Chaceon granulates reveals positive selection and novel genetic features

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Journal of Oceanology and Limnology 2020年第2期38卷 427-437页

作者： ZHANG Bo wu yingying WANG Xin JIANG Wei YIN Jianping LIN Qiang Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510275China Institution of South China Sea Ecology and Environmental Engineering Chinese Academy of SciencesGuangzhou 510275China University of Chinese Academy of Sciences Beijing 100049China Beijing Advanced Sciences and Innovation Center of Chinese Academy of Sciences Beijing 100049China Institute of Oceanology Chinese Academy of SciencesQingdao 266071China

Deep-sea organisms survive in an extremely harsh *** must be some genetic adaptation mechanisms for *** systematically characterized and compared the complete mitochondrial genome(mitogenome)of a deep-sea crab(Chaceon granulates)with those of shallow *** mitogenome of the crab was 16126 bp in length,and encoded 37 genes as most of a metazoan mitogenome,including 13 protein-coding genes(PCGs),22 transfer RNA(tRNA)genes,and 2 ribosomal RNA(rRNA)*** gene arrangement and orientation was conserved in the ***,a unique mitogenome element regulator,the origin of light-strand replication(O L),was firstly predicted in the present crab *** addition,further positive selection analysis showed that two residues(33 S in ND3 and 502 I in ND5)in *** mitogenome were positively selected,indicated the selective evolution of the deep-sea ***,the mitogenome of deep-sea *** showed a unique OL element and positive *** special features would influence the mitochondrial energy metabolism,and be involved in the adaptation of deepsea environment,such as oxygen deficits and low temperatures.

关键词： deep-sea organisms mitochondrial genome adaptation Chaceon granulates

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兰德公司治理模式与智库产品研究

竞争情报 2024年第3期20卷 47-54页

作者：姜杰陈海鹏吴颖颖潘镕上海市科学学研究所上海200031 上海大学上海200444

智库是推进国家治理体系和治理能力现代化的重要力量。完善智库治理结构、提升智库研究能力是新时代智库建设的内在要求。兰德公司是世界著名的智库,拥有一支多学科、高水平的人才队伍,完成了大批高质量的战略咨询报告。在治理结构上,... 详细信息

智库是推进国家治理体系和治理能力现代化的重要力量。完善智库治理结构、提升智库研究能力是新时代智库建设的内在要求。兰德公司是世界著名的智库,拥有一支多学科、高水平的人才队伍,完成了大批高质量的战略咨询报告。在治理结构上,兰德公司理事会为最高决策机构,为兰德公司提供重要的战略指导和商业决策支持,推动兰德公司持续发展;在能力提升上,兰德公司形成了一系列独特的研究方法,拥有一套完善的质量控制体系。这些对于提升我国智库治理能力和治理水平具有良好的参考和实践意义。

关键词：兰德公司智库治理产品

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足球灵敏素质测试综述

体育科技文献通报 2024年第3期32卷 75-78页

作者：董云飞王新胜吴盈盈广州体育学院广东广州510000

研究目的:在研读足球运动项目灵敏素质测试文献基础上,对足球灵敏素质测试方法进行分析和归纳总结,为足球运动项目灵敏素质测试研究提供参考。研究方法:本文主要运用了文献资料法、逻辑分析法。在足球项目中,运动员的灵敏素质是一项复... 详细信息

研究目的:在研读足球运动项目灵敏素质测试文献基础上,对足球灵敏素质测试方法进行分析和归纳总结,为足球运动项目灵敏素质测试研究提供参考。研究方法:本文主要运用了文献资料法、逻辑分析法。在足球项目中,运动员的灵敏素质是一项复杂的、综合性的身体素质之一,其测试方法主要有快速改变方向速度测试、反应灵敏测试。研究结果:①快速改变方向速度测试虽然需要的仪器设备较少,但是其测试过程缺乏感知决策,与足球运动实际不符。②反应灵敏测试虽然在测试过程中让受试者有了感知决策环节,更符合运动实际,但是也有其缺点。结论:目前国内在灵敏素质领域的研究与国外相比有较大差距。建议:密切关注国外在灵敏素质领域的研究成果,以补充完善相关知识。

关键词：足球灵敏素质测试

Screening White Spot Syndrome Virus(WSSV)-Resistant Molecular Markers from Fenneropenaeus chinensis

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Journal of Ocean University of China 2017年第1期16卷 129-136页

作者： wu yingying MENG Xianhong KONG Jie LUAN Sheng LUO Kun WANG Qingyin ZHENG Yongyun Marine Biology Institute of Shandong Province Qingdao 266002P.R.China Laboratory for Marine Fisheries Science and Food Production Processes of Qingdao National Laboratory for Marine Science and Technology Qingdao 266071P.R.China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071P.R.China

White spot syndrome virus(WSSV)-resistant molecular markers were screened from the selectively bred new variety ‘Huanghai No. 2' of Fenneropenaeus chinensis using unlabeled-probe high-resolution melting(HRM) technique. After the artificial infection with WSSV, the first 96 dead shrimps and the last 96 surviving shrimps were collected, representing WSSV-susceptible and-resistant populations, respectively. The genotypes at well-developed 39 single nucleotide polymorphisms(SNPs) loci were obtained. As revealed in the Chi-square test, 3 SNPs, genotype A/A of contig C364-89 AT, genotype A/A of C2635-527 CA and genotype C/T of contig C12355-592 CT, were positively correlated with disease-resistance traits. Other 2 SNPs, genotype G/G of contig C283-145 AG and genotype C/C of contig C12355-592 CT, were negatively correlated. Moreover, analysis with Blast X program for disease-resistant SNPs indicated that 3 contigs, Contig283, Contig364 and Contig12355, matched to the functional genes of effector caspase of Penaeus monodon, peptide transporter family 1-like protein, and 40 S ribosomal protein S2 of Perca flavescens with high sequence similarity. The results will be helpful to provide theoretical and technical supports for molecular marker-assisted selective breeding of F. chinensis.

关键词： Fenneropenaeu chinensis WSSV SNP molecular marker

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药护管理在神经内科高危药品管理中的效果

中国卫生产业 2024年第4期21卷 131-133,141页

作者：冯妙娜陈永坚伍莺莺台山市第二人民医院内一科广东台山529224 台山市第二人民医院药剂科广东台山529224

目的分析神经内科高危药品管理中药护管理的价值。方法选取2023年1—12月台山市第二人民医院的20名药师作为研究对象,2023年1—6月行传统管理为传统组,2023年7—12月行药护管理为管理组,对比两组管理质量、管理不当事件、不良反应、管... 详细信息

目的分析神经内科高危药品管理中药护管理的价值。方法选取2023年1—12月台山市第二人民医院的20名药师作为研究对象,2023年1—6月行传统管理为传统组,2023年7—12月行药护管理为管理组,对比两组管理质量、管理不当事件、不良反应、管理满意率。结果管理组的管理质量高于传统组,差异有统计学意义(P<0.05)。管理组管理不当事件总发生率为5.00%,低于传统组的20.00%,差异有统计学意义(P<0.05)。管理组的不良反应发生率为2.00%,低于传统组的9.00%,差异有统计学意义(P<0.05)。管理组的管理满意率为100.00%,高于传统组的75.00%,差异有统计学意义(P<0.05)。结论药护管理落实后能减少高危药品使用不当事件,还能避免不良反应发生,使管理质量得到大幅提升,可满足神经内科高危药品的管理需求。

关键词：神经内科满意率药护管理安全隐患高危药品