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Modulation of evening complex activity enables north-to-south adaptation of soybean

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Science China(Life Sciences) 2021年第2期64卷 179-195页

作者： Xiaolong Fang Yapeng Han Mengshi liu Jiacan Jiang Xiang Li Qichao Lian Xianrong Xie Yian Huang Qibin Ma Hai Nian Ji Qi Cunyi Yang yingxiang Wang State Key Laboratory of Genetic Engineering and Institute of Genetics Institute of Plant BiologySchool of Life SciencesFudan UniversityShanghai 200438China Guangdong Provincial Key Laboratory of Plant Molecular Breeding Guangdong Sub-center of National Center for Soybean ImprovementCollege of AgricultureSouth China Agricultural UniversityGuangzhou 510642China The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural UniversityGuangzhou 510642China

Soybean,a typical short-day crop,is sensitive to photoperiod,which is a major limiting factor defining its north-to-south cultivation *** long-juvenile(LJ)trait is controlled primarily by the J locus which has been used for decades by soybean breeders to delay flowering and improve grain yield in tropical *** J gene encodes an ortholog of the Arabidopsis Evening Complex(EC)component EARLY FLOWERING 3(ELF3).To identify modifiers of J,we conducted a forward genetic screen and isolated a mutant(eoj57)that in combination with j has longer flowering delay compared with j single mutant ***-based cloning and genome re-sequencing identified eoj57(designated as GmLUX2)as an ortholog of the Arabidopsis EC component LUX ARRHYTHMO(LUX).To validate that GmLUX2 is a modifier of J,we used trans-complementation and identified a natural variant allele with a similar *** also show that GmLUX2 physically interacts with GmELF3a/b and binds DNA,whereas the mutant and natural variant are attenuated in both *** analysis shows that the GmLUX2-GmELF3a complex co-regulates the expression of several circadian clock-associated genes and directly represses E1 *** results provide mechanistic insight into how GmLUX2-GmELF3 controls flowering time via synergistic regulation of gene *** novel insights expand our understanding of the regulation of the EC complex,and facilitate the development of soybean varieties adapted for growth at lower latitudes.

关键词： soybean flowering time circadian clock Evening Complex geographic adaptation

The E3 ubiquitin ligase CSIT1 regulates critical sterility-inducing temperature by ribosome-associated quality control to safeguard two-line hybrid breeding in rice

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Molecular Plant 2023年第10期16卷 1695-1709页

作者： Guoqing Peng Minglong liu Liya Zhu Wenlong Luo Qinghua Wang Mumei Wang Huiqiong Chen Ziliang Luo Yueping Xiao Yongjie Zhang Haona Hong Zhenlan liu Lingyan Zhou Guoqiang Guo yingxiang Wang Chuxiong Zhuang Hai Zhou State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangdong Laboratory for Lingnan Modern AgricultureKey Laboratory for Enhancing Resource Use Efficiency of Crops in South ChinaMinistry of Agriculture and Rural AffairsCollege of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China College of Agriculture&Biology Zhongkai University of Agriculture and EngineeringGuangzhou 510225China Guangdong Key Laboratory for New Technology Research of Vegetables Vegetable Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China Agronomy Department University of FloridaGainesvilleFL 32610USA Hengyang Academy of Agricultural Sciences Hengyang 421101China

Two-line hybrid breeding can fully utilize heterosis in *** thermo-sensitive genic male sterile(TGMS)lines,low critical sterility-inducing temperature(CSIT)is vital to safeguard the production of two-line hybrid seeds in rice(Oryza sativa),but the molecular mechanism determining CSIT is ***,we report the cloning of CSIT1,which encodes an E3 ubiquitin ligase,and show that CSIT1 modulates the CSIT of thermo-sensitive genic male sterility 5(tms5)-based TGMS lines through ribosome-associated quality control(RQC).Biochemical assays demonstrated that CSIT1 binds to the 80S ribosomes and ubiquitinates abnormal nascent polypeptides for degradation in the RQC *** of CSIT1 function inhibits the possible damage of tms5 to the ubiquitination system and protein translation,resulting in enhanced accumulation of anther-related proteins such as catalase to suppress abnormal accumulation of reactive oxygen species and premature programmed cell death in the tapetum,thereby leading to a much higher CSIT in the tms5-based TGMS *** together,our findings reveal a regulatory mechanism of CSIT,providing new insights into RQC and potential targets for future two-line hybrid breeding.

关键词： hybrid breeding thermo-sensitive genic male sterility critical sterility-inducing temperature ribosome-associated quality control programmed cell death

Experimental Nuclear Physics and Nuclear Chemistry—High-spin States in N52 Nuclei 92Zr and 93Nb

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近代物理研究所和兰州重离子加速器实验室年报：英文版 2009年第1期 29-29,31页

作者： liu Minliang Zhang Yuhu Qiang Yunhua Zhou Xiaohong Guo yingxiang Lei Xiangguo Supported by the National Natural Sciences Foundation the Major State Basic Research Development Program of China and the ChineseAcademy of Sciences.

Nuclear Shape-phase Diagrams

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近代物理研究所和兰州重离子加速器实验室年报：英文版 2007年第1期 44-45页

作者： liu Minliang Zhang Yuhu Zhou Xiaohong Guo yingxiang Lei Xiangguo Guo Wentao

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高血压病中医证型的聚类分析

天津中医药大学学报 2024年第8期43卷 730-735页

作者：孟令皓张傲崔淮星李迎香杨阳岳树娇刘颖王泓午步怀恩徐芳崔岩天津中医药大学公共卫生与健康科学学院天津301617 天津中医药大学中医学院天津301617

[目的]探索高血压病患者的证型分布及基本信息与证型的相关性。[方法]通过天津中医药大学第二附属医院内分泌科及心内科的高血压病患者,进行临床流行病学调查,收集4942例高血压病患者的基本信息和症状等,分析高血压病患者中医证候类型... 详细信息

[目的]探索高血压病患者的证型分布及基本信息与证型的相关性。[方法]通过天津中医药大学第二附属医院内分泌科及心内科的高血压病患者,进行临床流行病学调查,收集4942例高血压病患者的基本信息和症状等,分析高血压病患者中医证候类型分布特点。[结果]高血压病是一种临床上常见的慢性疾病,青年组不容易患此病,且青年组主要证型为肝火亢盛证,此病最高发人群为中老年人。中老年人身体各项机能逐渐衰退。研究发现:在肝肾阴虚证、肝火亢盛证、气虚痰浊证与瘀热互结证4组证型中,男性与女性有差异。在阴阳两虚证与瘀热互结证两组证型中,汉族与非汉族之间有差异。在肝肾阴虚证与气虚痰浊证两组证型中,青年组与中年组这两组都与老年组有差异;在肝火亢盛证中,青年组、中年组与老年组互相之间都有差异。最后在瘀热互结证中,青年组与老年组有差异。[结论]研究显示:高血压病更多以“热”象为主,主要涉及肝、肾、脾3脏,以头晕、疲倦、乏力、烦躁易怒、目干涩、视物昏花等为主症。且根据数据临床可分为五大证型,分别为阴阳两虚证、肝肾阴虚证、肝火亢盛证、气虚痰浊证、瘀热互结证。其中肝肾阴虚证和肝火亢盛证居临床多数。

关键词：聚类分析高血压病中医证型分布研究

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论计算档案学的出场逻辑

档案学通讯 2021年第5期 22-31页

作者：于英香刘茜上海大学图书情报档案系上海200444 中国人民大学电子文件管理研究中心北京100872

随着大数据与人工智能等新一代信息技术在档案领域的渗透,以及学科融合发展,催生了计算档案学这一新的研究领域,对其出场逻辑的探究,可深化学界对计算档案学的认知,推动计算档案学的发展。从生成逻辑看,计算档案学在档案学自身矛盾与社... 详细信息

随着大数据与人工智能等新一代信息技术在档案领域的渗透,以及学科融合发展,催生了计算档案学这一新的研究领域,对其出场逻辑的探究,可深化学界对计算档案学的认知,推动计算档案学的发展。从生成逻辑看,计算档案学在档案学自身矛盾与社会需求的双重作用下出场;从理论逻辑看,计算科学、信息科学与档案学之间的双向互动改变了档案学的研究走向,拓展了档案学的研究空间,推动了计算档案学的出场;从实践逻辑看,计算档案学实践对档案业务活动的优化提升了计算档案学的影响力,档案学教育变革与科研合作推动了计算档案学可持续发展。

关键词：计算档案学计算思维跨学科数字人文