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Chinese Science Bulletin 2003年第4期48卷 343-345页

作者： WANG Jiexi GAO Xin BAI Yan REN Huiming TAN yingxia ZHANG Yangpei Institute of Blood Transfusion Academy of Military Medical SciencesBeijing 100850China Department of Hematology The 307 HospitalBeijing 10039China

The serological and biochemical characteriza-tion of porcine red blood cells (pRBCs) are similar to human red blood cells. Porcine erythrocytes are considered as an alternative source for human blood transfusion. But there exist galactose-?,3-galactose antigens (Gal?,3Gal?, 4GalNAcR, abbreviated 酖al antigen) on pRBCs, which can induce anti-aGal antibodies in human serum. The aGal epitopes are the major antigen responsible for hyperacute rejection in xenotransfusion. In this study, recombined soy-bean -galactosidase (rS?GalE) was used to remove the aGal antigens from pPRCs for humanization. The results showed that aGal antigen was cleared by rS?GalE and the structure and function of rS?GalE treated pRBC were normal.

关键词： porcine red blood cell galactose-α1 3-galactose antigen recombined soybean α-galactosidase.

Preparation of CeO_2-Modified Mg(Al)O-Supported Pt–Cu Alloy Catalysts Derived from Hydrotalcite-Like Precursors and Their Catalytic Behavior for Direct Dehydrogenation of Propane

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Transactions of Tianjin University 2019年第2期25卷 169-184页

作者： yingxia Li Jiaxin Li Xiao Yang Xitao Wang Yanhong Xu Lihong Zhang School of Chemical Engineering and Technology Tianjin University Tianjin Key Laboratory of Applied Catalysis Science and Technology Tianjin University Department of Materials Engineering Xuzhou College of Industrial Technology

A series of PtCuCeMgAl quintuple hydrotalcite-like compounds with different Ce contents were synthesized by one-pot method. After calcining and reduction, CeO_2-modified Mg(Al)O-supported Pt–Cu alloy catalysts were obtained. To understand the effect of Cu and Ce, the structure and physico-chemistry properties of the catalysts were characterized and analyzed, and the catalytic behaviors were investigated in a direct dehydrogenation of propane to propene. The results show that the Pt^(4+), Cu^(2+), and Ce^(3+) ions can be incorporated into the brucite-like layers and the Ce content significantly affects the interaction strength between Pt and Cu and the dehydrogenation performance of propane. Under the reaction conditions, the highest propane conversion(45%) with 89% selectivity to propene and a 40% propene yield were achieved with a 0.3 wt% Ce-modified PtCu/Mg(Al)O catalyst. The improved catalytic performance is related to the easy formation of Pt–Cu alloy phase, excellent resistance to sintering, and coke deposits of active components modified by CeO_2.

关键词： Ce modification Pt–Cu alloy Propane dehydrogenation High dispersion Anti-sintering

Indole methylation protects diketopiperazine configuration in the maremycin biosynthetic pathway

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Science China Chemistry 2016年第9期59卷 1224-1228页

作者： yingxia Lan Yi Zou Tingting Huang Xiaozheng Wang Nelson L. Brock Zixin Deng Shuangjun Lin State Key Laboratory of Microbial Metabolism:Joint International Laboratory on Metabolic & Developmental Sciences School of Life Sciences & Biotechnology Shanghai Jiao Tong University Shanghai 200030 China

The maremycin biosynthetic gene cluster has been identified in Streptomyces sp. B9173. Comparative metabolic profiling with knockout mutant strains led to the identification of new products correlated to the maremycin biosynthesis, in particular the ＂demethyl＂-maremycins with an unexpected D-tryptophan unit. A biosynthetic pathway for the maremycins is proposed and plausible reasoning for tryptopban epimerization in the demethylmaremycin biosynthesis is also provided.

关键词： biosynthesis maremycin N-methylation methyltransferase epimerization

Early and Sensitive Detection of Pathogens for Public Health and Biosafety:An Example of Surveillance and Genotyping of SARS-CoV-2 in Sewage Water by Cas12a-Facilitated Portable Plasmonic Biosensor

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Research 2024年第2期2023卷 259-272页

作者： Tianzhong Li Yuzhi Chen Zhi Chen Yuan Hao Minyi liang yingxia Liu Guanyong Ou Huanian Zhang Yuxuan Tang Yabing Hao Swelm Wageh Omar AAl-Hartomy Abul Kalam Bin Zhang Xin Shi Xuejin Li Han Zhang College of Physics and Optoelectronic Engineering Institute of Translational MedicineDepartment of OtolaryngologyShenzhen Second People's HospitalFirst Affiliated Hospital of Shenzhen UniversityHealth Science CenterShenzhen UniversityShenzhen 518060China Shenzhen Key Laboratory of Sensor Technology Shenzhen 518060China The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's HospitalQingyuan 511518China Shenzhen Key Laboratory of Pathogen and Immunity National Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious DiseaseShenzhen Third People's HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen 518112China School of Medicine Southern University of Science and TechnologyShenzhen 518055China School of Physics and Optoelectronic Engineering Shandong University of TechnologyZibo 255049China Department of Physics Faculty of ScienceKing Abdulaziz University Jeddah 21589Saudi Arabia Research Center for Advanced Materials Science(RCAMS) King Khalid UniversityP.0.Box 9004Abha 61413Saudi Arabia Department of Chemistry College of ScienceKing Khalid UniversityP.0.Box9004Abha 61413 Saudi Arabia Health Sciences Institute China Medical UniversityShenyang 110000China The Chinese University of Hong Kong Shenzhen 518060China

Infectious diseases severely threaten public health and global *** addition to transmission through the air,pathogenic microorganisms have also been detected in environmental liquid samples,such as sewage *** biochemical detection methodologies are time-consuming and cost-ineffective,and their detection limits hinder early *** the present study,ultrafine plasmonic fiber probes with a diameter of 125μm are fabricated for clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)-12a-mediated sensing of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Single-stranded DNA exposed on the fiber surface is trans-cleaved by the Cas12a enzyme to release gold nanoparticles that are immobilized onto the fiber surface,causing a sharp reduction in the surface plasmon resonance(SPR)*** proposed fiber probe is virus-specific with the limit of detection of~2,300 copies/ml,and genomic copy numbers can be reflected as shifts in wavelengths.A total of 21 sewage water samples have been examined,and the data obtained are consistent with those of quantitative polymerase chain reaction(qPCR).In addition,the Omicron variant and its mutation sites have been fast detected using S gene-specific *** study provides an accurate and convenient approach for the real-time surveillance of microbial contamination in sewage water.

关键词： fiber consuming hinder

Ratiometric fluorescence immunoassay of SARS-CoV-2 nucleocapsid protein via Si-FITC nanoprobe-based inner filter effect

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Nano Research 2023年第4期16卷 5383-5390页

作者： Guobin Mao Yang Yang Shijie Cao Silu Ye Yifang Li Wei Zhao Hongwei An yingxia Liu Junbiao Dai Yingxin Ma CAS Key Laboratory of Quantitative Engineering Biology Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic GenomicsShenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Guangxi University of Chinese Medicine Nanning 530001China Shenzhen Key Laboratory of Pathogen and Immunity National Clinical Research Center for infectious diseaseState Key Discipline of Infectious DiseaseShenzhen Third People’s HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen 518112China

The global pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus has necessitated rapid,easy-to-use,and accurate diagnostic methods to monitor the virus ***,a ratiometric fluorescence enzyme-linked immunosorbent assay(ELISA)was developed using Si-fluorescein isothiocyanate nanoparticles(FITC NPs)for detecting SARSCoV-2 nucleocapsid(N)***-FITC NPs were prepared by a one-pot hydrothermal method using 3-aminopropyl triethoxysilane(APTES)-FITC as the Si *** method did not need post-modification and avoided the reduction in quantum yield and *** p-nitrophenyl(pNP)produced by the alkaline phosphatase(ALP)-mediated hydrolysis of pnitrophenyl phosphate(pNPP)could quench Si fluorescence in Si-FITC NPs via the inner filter *** ELISA,an immunocomplex was formed by the recognition of capture antibody/N protein/reporter ***-linked secondary antibody bound to the reporter antibody and induced pNPP hydrolysis to specifically quench Si fluorescence in Si-FITC *** change in fluorescence intensity ratio could be used for detecting N protein,with a wide linearity range(0.01-10.0 and 50-300 ng/mL)and low detection limit(0.002 ng/mL).The concentration of spiked SARS-CoV-2 N protein could be determined accurately in human ***,this proposed method can accurately distinguish coronavirus disease 2019(COVID-19)and non-COVID-19 patient ***,this simple,sensitive,and accurate method can be applied for the early diagnosis of SARS-CoV-2 virus infection.

关键词： Si-fluorescein isothiocyanate(FITC)nanoparticles ratiometric fluorescent probe severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) inner filter effect enzyme-linked immunosorbent assay(ELISA)

Rift Valley Fever Virus and Yellow Fever Virus in Urine: A Potential Source of Infection

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Virologica Sinica 2019年第3期34卷 342-345页

作者： Meng Li Beibei Wang Liqiang Li Gary Wong yingxia Liu Jinmin Ma Jiandong Li Hongzhou Lu Mifang liang Ang Li Xiuqing Zhang Yuhai Bi Hui Zeng BGI Education Center University of Chinese Academy of SciencesShenzhen 518083China Beijing Key Laboratory of Emerging Infectious Diseases Institute of Infectious DiseasesBeijing Ditan HospitalCapital Medical UniversityBeijing 100015China China National GeneBank BGI-ShenzhenShenzhen 518083China Shenzhen Key Laboratory of Pathogen and Immunity Guangdong Key Laboratory for Diagnosis and Treatment of Emerging Infectious DiseasesState Key Discipline of Infectious DiseaseSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen Third People's HospitalShenzhen 518112China Institute Pasteur of Shanghai Chinese Academy of SciencesShanghai 200031China Département de Microbiologie-Infectiologie et d'immunologie Université LavalQuébec G1V 0A6Canada Department of Internal Medicine Shanghai Medical CollegeFudan UniversityShanghai 200032China Key Laboratory of Medical Virology and Viral Diseases Ministry of HealthNational Institute for Viral Disease Control and Prevention (IVDC)Chinese Center for Disease Control and Prevention(China CDC)Beijing 102206China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyCenter for Influenza Research and Early-warning (CASCIRE)Chinese Academy of SciencesBeijing 100101China

Dear Editor,In recent years,the incidence of human infections caused by emerging or re-emerging pathogens has rapidly *** that were once regional now have the ability to spread globally in a short amount of time and pose a wider threat to public health(Weaver et al.2018).Yellow fever virus(YFV,family Flaviviridae,genus Flavivirus)is a mosquito-borne flavivirus that causes yellow fever in humans and has been endemic in Africa and Latin America for many years(Domingo et al.2018).

关键词： Yellow Fever Virus A Potential Source Infection

Detection of abnormalities in dyspneic patients using a new lung imaging modality

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Chinese Medical Journal 2014年第12期127卷 2247-2251页

作者： Wang Zhen Xiong yingxia Department of Emergency Medicine Beijing Shijitan Hospital Capital Medical UniversityBeijing 100038China

Background Although chest radiography is a useful examination tool,it has *** not all chest conditions can be detected on a radiograph,radiography cannot necessarily rule out all irregularities in the ***,further imaging studies may be required to clarify the results of a chest radiograph,or to identify abnormalities that are not readily *** aim of this study was to compare traditional chest radiography with acoustic-based imaging （vibration response imaging） for the detection of lung abnormalities in patients with acute *** The current investigation was a pilot *** sounds throughout the respiratory cycle were captured using an acoustic-based imaging *** patients who presented to the emergency department with acute dyspnea and a normal chest radiograph on admission were enrolled and underwent imaging at the time of *** and static images of vibration （breath sounds） and a dynamic image score were generated,and assessments were made using an evaluation *** In healthy volunteer controls （n=61）,the mean dynamic image score was 6.3±*** dyspneic patients with normal chest radiographs （n=51） and abnormal chest radiographs （n=48）,the dynamic image scores were 4.7±2.7 and 5.1±2.5,respectively （P ＜0.05）.The final assessment of the vibration images indicated abnormal findings in 15％,86％ and 90％ of the participants in the above groups,respectively （P ＜0.05）.Conclusions In patients with acute dyspnea who present with normal chest radiographs,respiratory sound analyses often showed abnormal ***,the ability of acoustic-based recordings to offer objective and noninvasive measurements of abnormal sound transmission may be useful in the clinical setting for patients presenting with acute dyspnea.

关键词： respiratory sounds vibration energy of respiratory sounds

Facile Conversion of Alcohols to Olefins by Tosylation and Subsequent SiO_2-promoted β-elimination

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Journal of Ocean University of China 2007年第2期6卷 196-198页

作者： RUAN Xiaohong SONG Gaopeng ZHANG Yichun LI yingxia Key Laboratory of Marine Drugs of the Ministry of Education of China School of Pharmacy Ocean University of China Qingdao 266003 P. R. China

A convenient and effective procedure was developed for the conversion of alcohol to olefin by tosylation and subsequent β-elimination promoted by silica gel in this study. Treatment of the alcohols with p-toluenesulfonyl chloride in pyridine at 0℃ af- fords tosylates which undergo β-elimination with silica gel in dichloromethane or chloroform at room temperature, yielding olefins with high productivity.

关键词： alcohol tosylation silica gel olefin β-elimination

Down-regulation of αGal epitopes by co-transfection of α1,3-galactosidase gene and α1,2-fucosyltransferase gene

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Chinese Science Bulletin 2005年第23期50卷 2723-2727页

作者： GONG Feng ZHANG Yangpei JIA Yanjun WANG Yingli TAN yingxia TIAN shuguang Beijing Institute of Transfusion Medicine Beijing 100850

The polycarbohydrate structure of Galα1- 3Galβ1-4GluNAc-R (known as αGal epitopes of xenoantigen), produced by α1-3-galactosyltransferase (α1,3-GT) in the course of animal development, is the major xenoantigen on the cell surface of porcine which causes hyperacute rejection in pig-to-human xenotransplantation. Alpha-1,3-galactosi- dase (AGL), a hydrolytic enzyme, can remove the terminal α-1,3-galactosyl from the Galα1-3Galβ1-4GluNAc-R struc-ture resulting in cleaning αGal epitopes from the porcine cells. Alpha-1,2-fucosyltransferase (HT) can modify the sur-face carbohydrate phenotype of porcine cells, bringing about reduction of αGal epitopes expression. In this study, human AGL and HT gene were co-transfected to porcine fetal fibro-blast (PFFb) in equimolar concentration to reduce the xeno-antigen. Gene and protein of hAGL and HT were both de-tected to express at high level by RT-PCR and Western blot, respectively. There was an 84% reduction in αGal xenoanti-gen and an 82% increase in H antigen as assayed by flow cytometry in the AGL and HT gene co-transfected PFFb. The number and morphology of transgenic PFFb chromosome were normal. Findings indicate that Galα1-3Gal epitopes of PFFb could be down regulated by AGL and HT co-transfec- tion without deleterious effects on the chromosomal profile of the transgenic cell.

关键词：异种移植术 αGal抗原决定基减量调节聚多糖 α1-3-半乳糖苷酶 α1-2-墨角藻糖基乳糖

Changes in nitrogen budget and potential risk to the environment over 20 years(1990–2010) in the agroecosystems of the Haihe Basin, China

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Journal of Environmental Sciences 2015年第2期27卷 195-202页

作者： Mengmeng Zheng Hua Zheng yingxia Wu Yi Xiao Yihua Du Weihua Xu Fei Lu Xiaoke Wang Zhiyun Ouyang State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Wuhan University of Technology Computer Network Information Center Chinese Academy of Sciences

The nitrogen balance can serve as an indicator of the risk to the environment of nitrogen loss from agricultural land. To investigate the temporal and spatial changes in agricultural nitrogen application and its potential threat to the environment of the Haihe Basin in China, we used a database of county-level agricultural statistics to calculate agricultural nitrogen input, output,surplus intensity, and use efficiency. Chemical fertilizer nitrogen input increased by 51.7% from1990 to 2000 and by 37.2% from 2000 to 2010, concomitant with increasing crop ***, the nitrogen surplus intensity increased by 53.5% from 1990 to 2000 and by16.5% from 2000 to 2010, presenting a continuously increased environmental risk. Nitrogen use efficiency decreased from 0.46 in 1990 to 0.42 in 2000 and remained constant at 0.42 in 2010,partly due to fertilizer composition and type improvement. This level indicates that more than half of nitrogen inputs are lost in agroecosystems. Our results suggest that although the improvement in fertilizer composition and types has partially offset the decrease in nitrogen use efficiency, the environmental risk has still increased gradually over the past 20 years, along with the increase in crop yields and nitrogen application. It is important to achieve a better nitrogen balance through more effective management to significantly reduce the environmental risk,decrease nitrogen surplus intensity, and increase nitrogen use efficiency without sacrificing crop yields.

关键词： Nitrogen budget Nitrogen surplus intensity Nitrogen use efficiency Environmental risk Agroecosystem Haihe Basin