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Quantitative Trait Loci for Panicle Layer Uniformity Identified in Doubled Haploid Lines of Rice in Two Environments

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Journal of Integrative Plant Biology 2009年第9期51卷 818-824页

作者： Liangyong Ma Jinsong Bao Longbiao Guo Dali Zeng Ximing Li Zhijuan Ji yingwu xia Changdeng Yang Qian Qian State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China Key Laboratory of Zhejiang Province and Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences Institute of Nuclear Agricultural Sciences Zhejiang University Hua Jiachi Campus Hangzhou 310029 China

Uniformity of stem height in rice directly affects crop yield potential and appearance, and has become a vital index for rice improvement. In the present study, a doubled haploid （DH） population, derived from a cross between japonica rice Chunjiang 06 and indica rice TN1 was used to analyze the quantitative trait locus （QTL） for three related traits of paniclelayer-uniformity; that is, the tallest panicle height, the lowest panicle height and panicle layer disuniforrnity in two locations：Hangzhou （HZ） and Hainan （HN）. A total of 16 QTLs for three traits distributed on eight chromosomes were detected in two different environments. Two QTLs, qTPH-4 and qTPH-8 were co-located with the QTLs for qLPH-4 and qLPH-8, which were only significant in the HZ environment, whereas the qTPH-6 and qLPH-6 located at the same interval were only significant in the HN environment. Two QTLs, qPLD-10.1 and qPLD-10.2, were closely linked to qTPH-10, and they might have been at the same locus. One QTL, qPLD-3, was detected in both environments, explaining more than 23% of the phenotypic variations. The CJ06 allele of qPLD-3 could increase the panicle layer disuniformity by 9.23 and 4.74 cm in the HZ and HN environments. Except for qPLD-3, almost all other QTLs for the same trait were detected only in one environment, indicating that these three traits were dramatically affected by environmental factors. The results may be useful for elucidation of the molecular mechanism of panicle-layer-uniformity and marker assisted breeding for super-rice.

关键词： double haploid population panicle layer uniformity plant height quantitative trait loci rice.

^(60)Co irradiation effect on cooked rice texture and amylopectin structure

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Nuclear Science and Techniques 1999年第3期10卷 176-179页

作者： BAO Jinsong SHU Qingyao Christine BERGMAN xia yingwu Anna McCLUNG (Institute of Nuclear Agricultural Sciences, Zhejiang University, Huajiachi,Hangzhou, 310029 China USDA/ARS, AT 7, Box 999, Beaumont, TX 77713, USA ) 浙江大学核农所浙江杭州 310029 浙江大学核农所浙江杭州 USDA/ARS 美国

The effect of γ irradiation on the cooked rice texture and amylopectin structure is studied with three varieties (Zhefu 504, Zhefu 802 and Zaogeng T2) subjected to 4kGy, skGy and 12kGy irradiation. The results indicate that irradiation has significant effect on the texture of the cooked rice; the hardness, cohesiveness,gumminess and chewiness reduce significantly with the increase of the irradiation *** also affects the chain length distribution of the amylopectin, the side-chains of amylopectin of Zhefu 504 with the 12DP, Zhefu 802 with 24DP13 and Zaogeng T2 with 24DP13 and 36DP25 are broken.

关键词： ^60Co辐照米饭组织胶淀粉结构稻米品质

Mapping QTLs for heading synchrony in a doubled haploid population of rice in two environments

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Journal of Genetics and Genomics 2009年第5期36卷 297-304页

作者： Liangyong Ma Changdeng Yang Dali Zeng Jing Cai Ximing Li Zhijuan Ji yingwu xia Qian Qian Jinsong Bao State Key Lab of Rice Biology China National Rice Research Institute Hangzhou 310006 China Key Laboratory of Zhejiang Province and Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences Institute of Nuclear Agricultural Sciences Zhejiang University Hangzhou 310029 China

Simultaneous heading of plants within the same rice variety, also refer to heading synchrony, is an important factor that affects simultaneous ripening of the variety. Understanding of the genetic basis of heading synchrony may contribute to molecular breeding of rice with simultaneous heading and ripening. In the present study, a doubled haploid （DH） population, derived from a cross between Chunjiang 06 and TN1 was used to analyze quantitative trait locus （QTL） for heading synchrony related traits, i.e., early heading date （EHD）, late heading date （LHD）, heading asynchrony （HAS）, and tiller number （PN）. A total of 19 QTLs for four traits distributed on nine chromosomes were detected in two environments. One QTL, qHAS-8 for HAS, explained 27.7% of the phenotypic variation, co-located with the QTLs for EHD and LHD, but it was only significant under long-day conditions in Hangzhou, China. The other three QTLs, qHAS-6, qHAS-9, and qHAS-10, were identified under short-day conditions in Hainan, China, each of which explained about 11% of the phenotypic variation. Two of them, qHAS-6 and qHAS-9, were co-located with the QTLs for EHD and LHD. Two QTLs, qPN-4 and qPN-5 for PN, were detected in Hangzhou, and qPN-5 was also detected in Hainan. However, none of them was co-located with QTLs for EHD, LHD, and HAS, suggesting that PN and HAS were controlled by different genetic factors. The results of this study can be useful in marker assisted breeding for improvement of heading synchrony.

关键词： rice doubled haploid population heading synchrony quantitative trait loci (QTL)

Analysis of variation in temperature-responsive leaf color mutant lines induced from Gamma irradiation in rice(Oryza sativa L.)

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Chinese Rice Research Newsletter 1997年第2期5卷 2-3页

作者： WU Dianxing SHU Qingyao xia yingwu LIU Guifu Inst of Nuclear Agri Sci,Zhejiang Agri Univ,Hangzhou 310029,China

Eight lines of temperature-responsive leaf colormutants induced by applying 300 Gy Gamma-ray irradiation to Thermo-sensitive genic malesterile line 2177s,were obtained through con-tinuous selection in seven generation... 详细信息

关键词： LCM Analysis of variation in temperature-responsive leaf color mutant lines induced from Gamma irradiation in rice Oryza sativa L

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植物转基因沉默及对策

生物技术 2000年第2期10卷 27-32页

作者：吴刚夏英武浙江大学核农所分子育种中心浙江杭州310029

转基因(transgene)是指所有通过基因工程手段构建,导入受体生物细胞并稳定整合到该受体细胞基因组中的外源基因.人们对植物进行遗传转化的最终目的是让转基因在受体植物基因组中得到稳定整合并在当代及其子代中得到有效、稳定的表达,但... 详细信息

转基因(transgene)是指所有通过基因工程手段构建,导入受体生物细胞并稳定整合到该受体细胞基因组中的外源基因.人们对植物进行遗传转化的最终目的是让转基因在受体植物基因组中得到稳定整合并在当代及其子代中得到有效、稳定的表达,但是由于存在多种影响因子和限制因素,使得转基因在受体植物中的表达往往事与愿违.现实证明,转基因在受体植物中往往不能稳定表达,有时甚至完全不表达,出现了所谓的转基因沉默现象(transgene silencing).转基因沉默并不等同于由于转基因在受体细胞中DNA序列的歧变或因位置效应引起的基因表达水平低下,也不同于转基因在有性世代分离中的丢失,而是指利用遗传转化方法导入并稳定整合进受体细胞中的完整的外源基因在当代转化体或在其后代中表达受到抑制的现象.

关键词：植物转基因沉默发生机理 TGS PTGS 防止措施

用近红外反射光谱测定小样本糙米粉的品质性状

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中国农业科学 1999年第4期32卷 94-99页

作者：舒庆尧吴殿星夏英武高明尉浙江大学原子核农业科学研究所

比较了用近红外反射光谱（ＮＩＲＳ）技术直接测定约０．５ｇ糙米粉和约３ｇ精米粉样本的表观直链淀粉含量（ＡＡＣ）、糊化温度（碱消值）和蛋白质含量的效果。结果发现，用精米粉和糙米粉样本测定蛋白质含量均有很好效果，建模时标准... 详细信息

比较了用近红外反射光谱（ＮＩＲＳ）技术直接测定约０．５ｇ糙米粉和约３ｇ精米粉样本的表观直链淀粉含量（ＡＡＣ）、糊化温度（碱消值）和蛋白质含量的效果。结果发现，用精米粉和糙米粉样本测定蛋白质含量均有很好效果，建模时标准误很小而决定系数高，校正标准误（ＳＥＣ）、检验工作标准误（ＳＥＰ）、校正决定系数（Ｒ２）以及检验决定系数（ＲＳＱ）依次分别为０．１１和０．１９，０．２０和０．２１，０．９９５和０．９３２以及０．９９和０．９１。在建立ＡＡＣ回归方程时，小样本糙米粉要比精米粉差一些，前者的ＳＥＣ、ＳＥＰ、Ｒ２和ＲＳＱ分别为０．９３３、２．０７、０．９２７和０．６５，而用精米粉时依次为０．４２６、０．９１、０．９８５和０．９３。用小样本糙米粉测定糊化温度（碱消值）时，ＳＥＣ、ＳＥＰ、Ｒ２和ＲＳＱ分别为０．３４９、０．４８、０．８２７和０．７４，而用精米粉时依次为０．２２４、０．３６、０．９４９和０．８４。在对水稻育种过程中用小样本糙米粉进行稻米品质的ＮＩＲＳ测定作了讨论。

关键词：近红外反射光谱蛋白质含量表观直链淀粉含量糊化温度(碱消值) 稻米品质

水稻品种功能Plus赋能乡村振兴和共同富裕

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中国稻米 2022年第5期28卷 57-61,65页

作者：吴殿星舒小丽张宁熊海铮夏英武浙江大学原子核农业科学研究所/农业农村部核农学重点实验室杭州310058

为满足未来农业及水稻产业可持续发展的需求,未来水稻育种需采用先进及综合的生物技术,以市场需求为导向,凸显“一个农艺或经济性状就是一项技术一个产业”的特征。本文综述了水稻品种重要“芯”性状、营养功能品质、种养结合生态模式... 详细信息

为满足未来农业及水稻产业可持续发展的需求,未来水稻育种需采用先进及综合的生物技术,以市场需求为导向,凸显“一个农艺或经济性状就是一项技术一个产业”的特征。本文综述了水稻品种重要“芯”性状、营养功能品质、种养结合生态模式、创意农业及跨界应用等方面的研究进展。

关键词：水稻育种功能乡村振兴

维普期刊数据库博看期刊

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空间技术诱发的水稻变异及育种价值

核农学报 1999年第3期13卷 175-178页

作者：吴殿星舒庆尧夏英武景晓阳浙江大学核农所作物诱变改良中心

空间技术诱变３个水稻中籼品种（系）表明，诱变当代ＳＰ１生理损伤轻，对发芽率、成苗率和育性基本无影响，但对苗高和株高影响较大，整个ＳＰ１群体表现抽穗不整齐。ＳＰ２叶色突变频率低，突变谱窄，其中遗传工程稻（ＥＲ１）未发现... 详细信息

空间技术诱变３个水稻中籼品种（系）表明，诱变当代ＳＰ１生理损伤轻，对发芽率、成苗率和育性基本无影响，但对苗高和株高影响较大，整个ＳＰ１群体表现抽穗不整齐。ＳＰ２叶色突变频率低，突变谱窄，其中遗传工程稻（ＥＲ１）未发现有叶色变异；早熟突变频率高而效应明显，同时因选用基因型的不同，ＳＰ２早熟突变的差异明显；伴随着早熟突变出现了诸如半矮秆、多蘖、粒重、粒色、叶色、叶形等其它农艺性状突变。除早熟性状外，突变性状稳定快且能真实遗传。突变体ＳＰＧＸ３表现早熟、优质、大穗、大粒且半矮秆，已直接和间接应用于早籼优质米育种。

关键词：水稻空间诱发早熟突变育种

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白菜抗虫基因转化受体体系的建立

园艺学报 2001年第2期28卷 175-176页

作者：谢建坤崔海瑞舒庆尧夏英武寿森炎浙江大学原子核农业科学研究所杭州310029 浙江大学园艺系杭州310029

就白菜基因转化受体系统的诸多因素进行了试验。

关键词：白菜子叶抗虫基因转化受体体系