检索结果-南通市图书馆

Deep learning-enabled fast DNA-PAINT imaging in cells

在线全文

学校读者我要写书评

暂无评论

Biophysics Reports 2023年第4期9卷 177-187页

作者： Min Zhu Luhao Zhang Luhong Jin Yunyue Chen Haixu Yang Baohua Ji yingke xu Department of Biomedical Engineering Key Laboratory of Biomedical Engineering of Ministry of Education State Key Laboratory of Extreme Photonics and Instrumentation Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine for Clinical Evaluation and Translational Research Zhejiang University Binjiang Institute of Zhejiang University Department of Engineering Mechanics Biomechanics and Biomaterials Laboratory Zhejiang University Department of Endocrinology Children's Hospital of Zhejiang University School of Medicine National Clinical Research Center for Children's Health

DNA-based point accumulation in nanoscale topography(DNA-PAINT) is a well-established technique for single-molecule localization microscopy(SMLM), enabling resolution of up to a few ***, DNA-PAINT involves the utilization of tens of thousands of single-molecule fluorescent images to generate a single super-resolution image. This process can be time-consuming, which makes it unfeasible for many researchers. Here, we propose a simplified DNA-PAINT labeling method and a deep learning-enabled fast DNA-PAINT imaging strategy for subcellular structures, such as microtubules. By employing our method, super-resolution reconstruction can be achieved with only onetenth of the raw data previously needed, along with the option of acquiring the widefield image. As a result, DNA-PAINT imaging is significantly accelerated, making it more accessible to a wider range of biological researchers.

关键词： DNA-PAINT U-Net SMLM Super-resolution imaging Deep learning

Novel function of biguanides in inhibition of phospholipase D1 expression via a translational mechanism in cancer cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2023年第5期10卷 1787-1790页

作者： Yuanfa Yao Hanbing Li Xinyi Wang Yuanhai Sun xuqin Zhao Weiwei Zha Jiaqiang Zhou Derek Toomre Junfen Fu yingke xu Department of Endocrinology Children's Hospital of Zhejiang University School of MedicineNational Clinical Research Center for Children's HealthHangzhouZhejiang 310051China Department of Biomedical Engineering Key Laboratory of Biomedical Engineering of Ministry of EducationState Key Laboratory of Modern Optical InstrumentationZhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness AppraisalZhejiang UniversityHangzhouZhejiang 310027China Institute of Pharmacology College of Pharmaceutical ScienceZhejiang University of TechnologyHangzhouZhejiang 310014China Department of Endocrinology The Affiliated Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhouZhejiang 310016China Department of Cell Biology Yale University School of MedicineNew HavenCT 06520USA Binjiang Institute of Zhejiang University HangzhouZhejiang 310053China

Phospholipase D(PLD)and its product phosphatidic acid(PA)function as pleiotropic factors in the regulation of cancer progression,which includes the promotion of cell growth,survival,cell migration,and angiogenesis.1 Chemical inhibition of the enzymatic activity of PLD1 and PLD2,or genetic down-regulation of their expression both induce tumor suppression,1 while the compounds for PLD expression inhibition are barely ***,we astonishingly found that the anti-hyperglycemic agents biguanides decreased PLD1 protein expression in cancer cells and further investigated its underlying regulatory mechanism.

关键词： cancer function translational

Development of a Wireless-Controlled LED Array for the Tunable Optogenetic Control of Cellular Activities

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering 2018年第6期4卷 745-747页

作者： Yuankai Qi Junye Chen xuechun Liu Xiaoxu Zhou Jiannan Fan Ping Shentu Olof Idevall-Hagren yingke xu Department of Biomedical Engineering Key Laboratory for Biomedical Engineering of Ministry of EducationZhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness AppraisalZhejiang UniversityHangzhou 310027China Department of Hepatobiliary and Pancreatic Surgery The Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China Department of Medical Cell Biology Uppsala Biomedical CentreUppsala UniversityUppsala 75123Sweden

*** In order to decipher a complex biological process,tools are required to perturb the various players involved to gain information about the important *** modules are genetically encoded molecular reagents that,when expressed in cells,allow a specific biological process to be precisely controlled by light in a spatiotemporal manner[1].Optogenetics thus offers cell biologists an unprecedented new way to perturb cellular *** application of optogenetic approaches in cellular biology and synthetic biology research has evolved tremendously in the last few years[2–4].

关键词： Development Wireless-Controlled LED Array Cellular Activities

U-Net-based deep learning for tracking and quantitative analysis of intracellular vesicles in time-lapse microscopy images

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative Optical Health Sciences 2022年第5期15卷 63-73页

作者： Zhichao Liu Heng Zhang Luhong Jin Jincheng Chen Alexander Nedzved Sergey Ablameyko Qing Ma Jiahui Yu yingke xu Department of Biomedical Engineering MOE Key Laboratory of Biomedical Engineering State Key Laboratory of Modern Optical Instrumentation Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal Zhejiang University Hangzhou 310027P.R.China Alibaba-Zhejiang University Joint Research Center of Future Digital Healthcare Hangzhou 310027P.R.China National Academy of Sciences United Institute of Informatics Problems Belarusian State University Minsk 220012Republic of Belarus§Hangzhou Dowell Photonics Measurement Company Limited Hangzhou 310000P.R.China Binjiang Institute of Zhejiang University Hangzhou 310053 P.R.China Department of Endocrinology Children's Hospital of Zhejiang University School of Medicine National Clinical Research Center for Children's Health Hangzhou 310051 China

Fluorescence microscopy has become an essential tool for biologists,to visualize the dynamics of intracellular structures with specific *** measuring the dynamics of moving objects inside the cell is pivotal for understanding of the underlying regulatory ***-containing vesicles are involved in various biological processes such as material transportation,organelle interaction,and hormonal regulation,whose dynamic characteristics are signi¯cant to disease diagnosis and drug *** some algorithms have been developed for vesicle tracking,most of them have limited performance when dealing with images with low resolution,poor signal-to-noise ratio(SNR)and complicated ***,we proposed a novel deep learning-based method for intracellular vesicle *** trained the U-Net for vesicle localization and motion classification,with demonstrates great performance in both simulated datasets and real biological *** combination with fan-shaped tracker(FsT)we have previously developed,this hybrid new algorithm significantly improved the performance of particle tracking with the function of subsequently automated vesicle motion ***,its performance was further demonstrated in analyzing with vesicle dynamics in different temperature,which achieved reasonable ***,we anticipate that this novel method would have vast applications in analyzing the vesicle dynamics in living cells.

关键词： Deep learning image processing vesicle tracking fluorescence microscopy U-Net.

超分辨显微成像中荧光单分子定位算法的研究进展

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国生物医学工程学报 2020年第2期39卷 229-237页

作者：林婉妮金璐红许迎科浙江大学生物医学工程系生物医学工程教育部重点实验室浙江省心脑血管检测技术与药效评价重点实验室杭州310027

超分辨显微成像技术的发展,可为生物医学的研究提供前所未有的机会。基于单分子定位的超分辨显微成像技术由于其相对简单的硬件结构,在生物医学领域的应用较为广泛。单分子定位的超分辨成像巧妙利用荧光发光的特点,针对少量离散的随机... 详细信息

超分辨显微成像技术的发展,可为生物医学的研究提供前所未有的机会。基于单分子定位的超分辨显微成像技术由于其相对简单的硬件结构,在生物医学领域的应用较为广泛。单分子定位的超分辨成像巧妙利用荧光发光的特点,针对少量离散的随机发光的荧光分子进行成像,再通过拟合分析实现单分子的高精度空间定位。在这一过程中,荧光单分子的定位算法及图像处理速度显得尤其关键。对单分子定位成像算法的基本原理进行阐述,包括模型和估计器的选择以及重建结果的评估等内容。同时针对拟合方法和功能的不同,对10余种算法进行分类和比较,并选取具有代表性的算法展开讨论和分析,希望对未来开展相关研究提供一定的参考。

关键词：单分子定位算法超分辨显微成像点扩散函数图像处理

在线全文

学校读者我要写书评

暂无评论

光遗传学技术用于调控细胞信号通路的研究

中国细胞生物学学报 2015年第11期37卷 1560-1565页

作者：南迪许迎科浙江大学生物医学工程系杭州310027 生物医学工程教育部重点实验室浙江省心脑血管检测技术与药效评价重点实验室杭州310027

光遗传学技术结合蛋白的遗传学表达与激光的光控和成像,可实现对细胞内特定信号通路分子的快速激活与调控,在细胞生物学的研究中具有广阔的应用前景。至今为止,越来越多的光控元件被发现,它们具备不同的结构特征及光反应特性,极大的扩... 详细信息

光遗传学技术结合蛋白的遗传学表达与激光的光控和成像,可实现对细胞内特定信号通路分子的快速激活与调控,在细胞生物学的研究中具有广阔的应用前景。至今为止,越来越多的光控元件被发现,它们具备不同的结构特征及光反应特性,极大的扩展了光遗传学技术的生物医学应用。该综述将对不同种类的光控元件及其应用于活细胞中信号通路调控的研究进行总结。

关键词：光遗传学光敏蛋白信号转导

在线全文

学校读者我要写书评

暂无评论

深度学习在超分辨显微成像中的应用研究进展

激光与光电子学进展 2021年第24期58卷 92-105页

作者：陆青霜金璐红许迎科浙江经贸职业技术学院人文旅游系浙江杭州310018 浙江大学生物医学工程系生物医学工程教育部重点实验室浙江省心脑血管检测技术与药效评价重点实验室浙江杭州310027

荧光超分辨显微成像技术的发展使得人们有望以前所未有的时空分辨率来实现细胞内动态生命活动的纳米尺度探测。传统超分辨显微成像技术需要较强的激发光源或采集数量较多的原始数据用于图像重建,因此在活细胞动态实时成像应用中存在不... 详细信息

荧光超分辨显微成像技术的发展使得人们有望以前所未有的时空分辨率来实现细胞内动态生命活动的纳米尺度探测。传统超分辨显微成像技术需要较强的激发光源或采集数量较多的原始数据用于图像重建,因此在活细胞动态实时成像应用中存在不足。深度学习驱动的超分辨成像新技术的研究,有望在多个方面突破现有超分辨成像技术的发展瓶颈。从超分辨成像技术的原理出发,分析了传统超分辨成像技术的不足,总结阐述了深度学习技术在超分辨显微成像领域中的最新应用及未来的研究方向与面临的挑战。

关键词：医用光学荧光成像超分辨显微成像深度学习图像重建

在线全文

学校读者我要写书评

暂无评论

中国高校绩效评价指标体系比较与启示

未来与发展 2017年第5期38卷 43-50页

作者：徐颖科河南财经政法大学河南郑州450046

本文对我国政府部门和非政府组织提出的多个高校绩效评价指标体系进行了研究,发现高校绩效评估理论基础比较薄弱,绩效评估持续进行取决于政府部门的愿意,没有建立评估法律制度,绩效指标重视投入和科研评价忽视了高校多样性。应该根据我... 详细信息

本文对我国政府部门和非政府组织提出的多个高校绩效评价指标体系进行了研究,发现高校绩效评估理论基础比较薄弱,绩效评估持续进行取决于政府部门的愿意,没有建立评估法律制度,绩效指标重视投入和科研评价忽视了高校多样性。应该根据我国高校发展实际,针对不同类型的高校所分类评估,在保持共性指标的同时,设计彰显个性特征的评估指标体系,同一指标在权重、不同学科性质的观测点须有所不同。引导高校办出特色,提升办学质量和绩效水平。

关键词：绩效评价指标体系分类评估

在线全文

学校读者我要写书评

暂无评论

基于灰色关联分析的高校财务绩效评价研究

新疆财经大学学报 2017年第4期 46-55页

作者：徐颖科河南财经政法大学河南郑州450046

本文从财政预算编制能力、收入能力、运行能力和偿债能力四个维度构建高校财务绩效评价体系,依据灰色关联原理对指标进行客观赋权,并用河南省22所高校的相关数据进行实证分析。研究发现:河南高校的财务绩效整体较好,多数高校处于良好和... 详细信息

本文从财政预算编制能力、收入能力、运行能力和偿债能力四个维度构建高校财务绩效评价体系,依据灰色关联原理对指标进行客观赋权,并用河南省22所高校的相关数据进行实证分析。研究发现:河南高校的财务绩效整体较好,多数高校处于良好和合格水平;医学类高校的财务绩效最好,财经类高校的财务绩效相对较差。今后可通过降低预算偏离、拓宽资金来源、化解高校债务和调整支出结构、提高支出效益等举措改善高校财务绩效。

关键词：绩效评价灰色理论预算偏离