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Journal of Integrative Plant Biology 2014年第7期56卷 622-634页

作者： Lei Wan Xiuwen Wang Shaoqing Li Jun Hu Wenchao Huang yingguo zhu State Key Laboratory of Hybrid Rice College of Life Sciences Wuhan University

Katanin, a microtubule-severing enzyme, consists of two subunits：the catalytic subunit P60, and the regulatory subunit P80. In several species, P80 functions in meiotic spindle organization, the flagella biogenesis, the neuronal development, and the male gamete production. However, the P80 function in higher plants remains elusive. In this study, we found that there are three katanin P80 orthologs （OsKTN80a, OsKTN80b, and OsKTN80c） in Oryza sativa L. Overexpression of OsKTN80a caused the retarded root growth of rice seedlings. Further investigation indicates that the retained root growth was caused by the repressed cell elongation in the elongation zone and the stalled cytokinesis in the division zone in the root tip. The in vivo examination suggests that OsKTN80a acts as a microtubule stabilizer. We＆amp;nbsp;prove that OsKTN80a, possibly associated with OsKTN60, is involved in root growth via regulating the cell elongation and division.

关键词： Cell division cell expansion Oryza sativa OsKTN8oa rootgrowth

大鼠密闭管道内爆炸烧冲复合伤及重要器官损伤的观察研究

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陆军军医大学学报 2024年第12期46卷 1323-1335页

作者：翁昌梅杨光明张双博朱英国程祥云段朝霞康建毅王建民陆军军医大学(第三军医大学)大坪医院武器杀伤生物效应评估研究室创伤与化学中毒全国重点实验室重庆400042

目的采用密闭管道内气体爆炸方式,建立热辐射与冲击波复合致伤模型,探讨爆炸烧冲复合因素致大鼠重要器官伤情特点。方法8~10周龄雄性SD大鼠72只,体质量(200±10)g,随机分为对照组、轻度组、重度组。采用爆炸冲击波与热辐射致伤系统致大... 详细信息

目的采用密闭管道内气体爆炸方式,建立热辐射与冲击波复合致伤模型,探讨爆炸烧冲复合因素致大鼠重要器官伤情特点。方法8~10周龄雄性SD大鼠72只,体质量(200±10)g,随机分为对照组、轻度组、重度组。采用爆炸冲击波与热辐射致伤系统致大鼠不同程度损伤,并进行物理参数测试,伤后存活大鼠进行呼吸功能检测,24 h后解剖并检测血气、血液生化及血清炎症因子、肺损伤相关蛋白等指标,并观察肺组织及气管病理学形态变化情况。结果爆炸冲击波超压峰值范围为209~493 kPa,温度为152~258℃;轻度组死亡率为8.3%,重度组死亡率为53.1%;与对照组比较,伤后6 h内大鼠吸气时间(inspiratory time,Ti)、潮气量(Tidal Volume,TV)、松弛时间(relaxation time,Tr)增高(P<0.05),呼吸频率(respiratory rate,f)降低(P<0.05);伤后24 h二氧化碳分压(PaCO_(2))、碱剩余值(BE)、碳酸氢根(HCO_(3)^(-))值显著增高(P<0.05),氧分压(PaO_(2))显著降低(P<0.05);血清丙氨酸转氨酶(alanine transaminase,ALT)、天冬氨酸氨基转氨酶(aspartate aminotransferase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)、总胆红素(total bilirubin,TBI)、总胆汁酸(total bile acid,TBA)水平均显著增高(P<0.05),白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、IL-6水平均显著增高(P<0.01);伤后大鼠气管黏膜层有不同程度的出血灶及发炎;肺损伤发生率高且病理表现为点片状出血至整肺叶弥漫性出血,肺泡间隔断裂,间隔增厚,红细胞及炎性细胞浸润,血清肺损伤相关蛋白水平显著增高(P<0.05)。结论建立出高度模拟密闭管道内轻至重度爆炸烧冲复合伤、能够满足研究需要的大鼠模型;爆炸烧冲复合伤以肺损伤、呼吸功能障碍、肝脏损伤及继发性感染为主。

关键词：爆炸冲击波烧冲复合伤急性肺损伤

细胞器应激反应与自噬及铁死亡参与氯化钴诱导的血管平滑肌细胞损伤

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中国临床药理学与治疗学 2024年第1期29卷 1-10页

作者：雷艳彭小勇邓蒙生张东冬朱英国王建民段朝霞李涛刘良明杨光明陆军军医大学陆军卫勤训练基地战救技能训练教研室重庆400042 陆军特色医学中心战伤休克与输血研究室重庆400042 陆军特色医学中心武器杀伤生物效应评估研究室创伤、烧伤与复合伤国家重点实验室重庆400042

目的:综合采用生物信息学分析和体外细胞实验验证,研究化学性低氧诱导剂氯化钴(CoCl2)诱导血管平滑肌细胞损伤与细胞器应激反应及自噬性死亡(自噬)和铁死亡之间的关系。方法:从基因表达数据库(GEO)中获取CoCl2处理血管平滑肌细胞的基因... 详细信息

目的:综合采用生物信息学分析和体外细胞实验验证,研究化学性低氧诱导剂氯化钴(CoCl2)诱导血管平滑肌细胞损伤与细胞器应激反应及自噬性死亡(自噬)和铁死亡之间的关系。方法:从基因表达数据库(GEO)中获取CoCl2处理血管平滑肌细胞的基因芯片数据集GSE119226,采用R语言分析数据,探讨CoCl2处理与细胞器应激反应(高尔基体应激、内质网应激)及两种细胞死亡方式(铁死亡、自噬性死亡)间的关系。采用原代培养的大鼠血管平滑肌细胞(rVSMC)和CoCl2诱导的体外缺氧模型,CCK-8法检测细胞活力变化,DCFH-DA法检测细胞内活性氧水平,Western blot法检测缺氧关键分子HIF-1α、高尔基体应激标志物GM130与p115、内质网应激标志物GRP78与CHOP、自噬标志物LC3-Ⅱ/LC3-Ⅰ与Beclin1以及铁死亡标志物GPx4与xCT的表达水平,并观察给予相应的处理剂来诱导或抑制细胞器应激反应或细胞死亡对CoCl2诱导的细胞损伤作用的影响。结果:对GSE119226数据集进行差异表达基因筛选分析,结果显示CoCl2处理血管平滑肌细胞对细胞器功能与应激反应、自噬和铁死亡相关基因有明显影响,其中内质网应激、内质网蛋白加工、高尔基体对质膜蛋白转运的调控、自噬/自噬性死亡、铁离子调节与铁死亡等是主要富集的信号通路和生物学过程。体外实验结果显示,与培养的正常对照rVSMC相比,CoCl2处理后细胞活力明显降低,细胞内HIF-1α蛋白表达与活性氧水平升高。CoCl2处理后,rVSMC中反映高尔基体应激发生的高尔基体结构蛋白GM130与p115的表达水平降低,反映内质网应激发生的标志分子GRP78与CHOP升高;同时CoCl2也使细胞自噬标志物LC3-Ⅱ/LC3-Ⅰ与Beclin1水平降低,提示自噬水平降低,而铁死亡标志物GPx4与xCT的表达水平降低则提示细胞发生铁死亡。与CoCl2处理组相比,诱导高尔基体应激、内质网应激或铁死亡可使细胞活力进一步降低,而抑制上述过程可改善细胞活力;另一方面,升高自噬水平可改善CoCl2降低的细胞活力。结论:CoCl2诱导低氧可导致血管平滑肌细胞损伤,高尔基体应激、内质网应激和铁死亡发生以及自噬水平降低在其中起重要作用,抑制细胞器应激反应和铁死亡或升高自噬水平可改善CoCl2导致的血管平滑肌细胞缺氧损伤。

关键词：氯化钴高尔基体应激内质网应激自噬铁死亡

Characterization and Use of Male Sterility in Hybrid Rice Breeding

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Journal of Integrative Plant Biology 2007年第6期49卷 791-804页

作者： Shaoqing Li Daichang Yang yingguo zhu Key Laboratory of MOE for Plant Developmental Biology and Institute of Genetics College of Life Sciences Wuhan University Wuhan 430072 China

The hybrid rice （Oryza sativa L.） breeding that was Initiated In China in the 1970s led to a great improvement in rice productivity. In general, It increases the grain yield by over 20% to the inbred rice varieties, and now hybrid rice has been widely introduced into Africa, Southern Asia and America. These hybrid varieties are generated through either three-line hybrid and two-line hybrid systems; the former is derived from cytoplasmic male sterility （CMS） and the latter derived from genlc male sterility （GMS）. There are three major types of CMS （HL, BT and WA） and two types of GMS （photoperlod-sensitlve （PGMS） and temperature-sensitive （TGMS））. The BT- and HL-type CMS genes are characterized as orf79 and orfH79, which are chimeric toxic genes derived from mltochondrial rearrangement. Rf3 for CMS-WA Is located on chromosome 1, while Rf1, Rf4, Rf5 and Rf6 correspond to CMS-BT, CMSoWA and CMS- HL, located on chromosome 10. The Rfl gene for BT-CMS has been cloned recently, and encodes a mltochondriatargeted PPR protein. PGMS Is thought to be controlled by two recessive loci on chromosomes 7 and 12, whereas nine recessive alleles have been identified for TGMS and mapped on different chromosomes. Attention Is still urgently needed to resolve the molecular complexity of male sterility to assist rice breeding.

关键词： hybrid rice male sterility Oryza sativa.

The editing sites in transcripts of functional genes of rice mitochondria

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Chinese Science Bulletin 2002年第11期47卷 906-909页

作者： Yl Ping WANG Li SUN Qingping zhu yingguo Key Laboratory of MOE for Plant Developmental Biology and Institute of Genetics College of Life SciencesWuhan UniversityWuhan 430072China

RNA editing exists extensively in the higher plant mitochondria, and is a required step for forming functional proteins. There may be some relationship between RNA editing and cytoplasmic male sterility (CMS), a kind of phenomenon that is attributed to mitochondrial genome mutations. The research materials used are the gametophytic male sterility line (A), maintainer line (B) and F1 hybrid (F1) of HL-type CMS rice. cDNAs and DNAs of atp6 and coxII have been obtained from A, B and F1 by PCR and RT-PCR. Comparing sequences of cDNAs and DNAs, 18 and 15 editing sites were found respectively in the transcripts of atp6 and coxll. A, B and F1 shared the same editing sites. RNA editing improves hydrophobicity and conservation of the predicted protein as compared with other organisms.

关键词： mitochondria RNA editing editing sites cytoplasmic male sterility.

Discovery of mitochondrial chimeric-gene associated with cytoplasmic male sterility of HL-rice

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Chinese Science Bulletin 2002年第9期47卷 744-747页

作者： Yl Ping WANG Li SUN Qingping zhu yingguo The Key Laboratory of Ministry of Education for Plant Developmental Biology and Institute of Genetics College of Life SciencesWuhan UniversityWuhan 430072China

The mitochondrial genome libraries of HL-type sterile line(A) and maintainer line(B) have been constructed. Mitochondrial gene, atp6, was used to screen libraries, due to the different Southern and Northern blot results between sterile and maintainer line. Sequencing analysis of positive clones proved that there were two copies of atp6 gene in sterile line and only one in maintainer line. One copy of atpt6 in sterile line was same to that in maintainer line; the other showed different flanking sequence from the 49th nucleotide downstream of the termination codon of atp6 gene. A new chimeric gene, orfH79, was found in the region. OrfH79 had homology to mitochondrial gene cox Ⅱ and orf107, and was special to HL-sterile cytoplasm.

关键词： cytoplasmic male sterility mitochondria chimeric gene.

Mapping of the nuclear fertility restorer gene for HL cytoplasmic male sterility in rice using microsatellite markers

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Chinese Science Bulletin 2000年第5期45卷 430-432页

作者： HUANG Qingyang HE Yuqing JING Runchun zhu Renshan zhu yingguo College of Life Science Wuhan UniversityWuhan 430072China College of Life Science Central China Normal UniversityWuhan 430079China

Bulked segregant analysis (BSA) of a BC1 population derived from Congguang 41A//Miyang 23/Congguang 41B was used to map the nuclear fertility restorer gene for Honglian (HL) cytoplasmic male sterility. One hundred and fifty-nine microsatellite primer pairs were screened for polymorphisms between the parents and between two bulks representing fertile and sterile plants. One microsatellite marker RM258 produced polymorphic products. The nuclear fertility restorer gene for HL cytoplasmic male sterility was mapped on chromosome 10, 7.8cM from RM258. The restorer gene may be clustered on chromosome.

关键词： rice HL cytoplasmic male sterility restorer gene microsatellite marker.

The mechanism of ORFH79 suppression with the artificial restorer fertility gene Mt-GRP162

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The mechanism of ORFH79 suppression with the artificial rest...

Maxie A- a novel cytoplasm male sterile line

中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： Jun Hu Wenchao Huang Qi Huang Xiaojian Qin Zhiwu Dan GuoXin Yao Renshan zhu yingguo zhu State Key Laboratory of Hybrid Rice College of Life SciencesWuhan University

The restoration fertility complex(RFC) was previously identified in Honglian (HL)-cytoplasmic male sterility(CMS) rice(Oryza sativa),and glycine-rich protein 162(GRP162) is responsible for binding to the CMS-associated transcriptatp6-orfH79,「Here,we engineered a recombinant GRP162 containing the mitochondrial transit peptide,termed Mt-GRP162,as an artificial restorer of fertility(Rf) gene.Mt-GRP162 was confirmed to bind to CMS-associated RNA and to localize to the mitochondria.The transgenic plants showed restored fertility with partially functional pollen,「We found that the expression of ORFH79 decreased in transgenic plants,while the expression of atp6-orfH79 was not changed.These data indicate that Mt-GRPI62 restores fertility by suppressing the expression of the cytotoxic protein ORFH79 at the post-transcriptional level rather than via the cleavage of atp6-orfH79 in the presence of RFC.「These findings contribute to our understanding of the mechanisms of restoration through diverse pathways.

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Chinese Rice Research Newsletter 1997年第1期5卷 4-5页

作者： ZHANG Xiaoguo and zhu yingguo, College of Life Sciences, Wuhan Univ, Wuhan 430072, China

The pollen abortion of Maxie A and Zhengshan97 A belong to stainless type with Ksolu-tion.The maiority of Maxie A abortive Pollenstook cireular shape and few was staining abortion

关键词： Maxie A-a novel cytoplasm male sterile line than PSS

Genetic analysis of fertility restoration of Maxie cytoplasmic male sterility in rice(Oryza sativa L.)

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Chinese Rice Research Newsletter 1996年第4期4卷 1-2页

作者： ZHANG Xiaoguo zhu yingguo and MEI Qiming,College of Life Sci,Wuhan Univ,Wuhan 430072,China

The genetic behavior of fertility restorationgenes of the cytoplasmic male sterile line Max-ie A was studied to facilitate the use ofMaweizhan male sterile cytoplasm.The F,F,and Fof Maxie A/Minhui 63 were grownin padd... 详细信息

关键词： Oryza sativa L Genetic analysis of fertility restoration of Maxie cytoplasmic male sterility in rice