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Identifying the r.sk factor. for.p.ncr.atic fistula after.lap.r.scop.c p.ncr.aticoduodenectomy in p.tients with p.ncr.atic cancer

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Wor.d Jour.al of Gastr.intestinal Sur.er. 2024年第6期16卷 1609-1617页

作者： Hang Xu Qing-cai Meng Jie Hua Wei Wang department of p.ncr.atic Sur.er. Fudan University Shanghai Cancer CenterShanghai 200032China department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China

BACKGr.UND Lap.r.scop.c p.ncr.aticoduodenectomy(Lp.)is a sur.ical p.ocedur. for.tr.ating p.ncr.atic cancer.however.the r.sk of comp.ications r.mains high owing to the wide r.nge of or.ans involved dur.ng the sur.er. and the difficulty of *** fistula(p.)is a major.comp.ication that not only incr.ases the r.sk of p.stop.r.tive infection and abdominal hemor.hage but may also cause multi-or.an failur.,which is a ser.ous thr.at to the p.tient’s *** study hyp.thesized the r.sk factor. for.p. after.*** To identify the r.sk factor. for.p. after.lap.r.scop.c p.ncr.atoduodenectomy in p.tients with p.ncr.atic *** We r.tr.sp.ctively analyzed the data of 201 p.tients admitted to the Fudan university shanghai Cancer.Center.between August 2022 and August 2023 who under.ent Lp. for.p.ncr.atic *** the basis of the p.’s incidence(gr.des B and C),p.tients wer. categor.zed into the p.(n=15)and non-p. gr.up.(n=186).Differ.nces in gener.l data,p.eop.r.tive labor.tor. indicator.,and sur.er.-r.lated factor. between the two gr.up. wer. comp.r.d and analyzed using multifactor.al logistic r.gr.ssion and r.ceiver.op.r.ting char.cter.stic(r.C)cur.e *** The p.op.r.ions of males,combined hyp.r.ension,soft p.ncr.atic textur.,and p.ncr.atic duct diameter.3 mm;sur.er. time;body mass index(BMI);and amylase(Am)level in the dr.inage fluid on the fir.t p.stop.r.tive day(Am>1069 U/L)wer. gr.ater.in the p. gr.up.than in the non-p. gr.up.p.0.05),wher.as the p.eop.r.tive monocyte count in the p. gr.up.was lower.than that in the non-p. gr.up.all p.0.05).The logistic r.gr.ssion analysis r.vealed that BMI>24.91 kg/m²[odds r.tio(Or.=13.978,95%confidence inter.al(CI):1.886-103.581],hyp.r.ension(Or.8.484,95%CI:1.22-58.994),soft p.ncr.atic textur.(Or.42.015,95%CI:5.698-309.782),and op.r.tion time>414 min(Or.15.41,95%CI:1.63-145.674)wer. r.sk factor. for.the develop.ent of p. after.Lp. for.p.ncr.atic cancer.all p.0.05).The ar.as under.the r.C cur.e for.BMI,

关键词： p.ncr.atic cancer Lap.r.scop. p.ncr.aticoduodenectomy p.ncr.atic fistula r.sk factor. r.ceiver.op.r.ting char.cter.stic cur.e

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MCAO大鼠MMp.9蛋白表达及补阳还五汤干预作用

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复旦学报（医学版） 2003年第5期30卷 470-473页

作者：高颖蔡定芳复旦大学附属中山医院中医科上海200032

目的　探讨脑缺血后MMp.9蛋白表达的时程改变及补阳还五汤对其调节作用。方法　采用MCAO大鼠模型 ,设假手术组、模型组和模型 +补阳还五汤组 ,应用明胶酶谱法于再灌后 1、3、2 4、4 8h和 5d测定MMp.9蛋白表达。结果　①假手术组未检测到... 详细信息

目的　探讨脑缺血后MMp.9蛋白表达的时程改变及补阳还五汤对其调节作用。方法　采用MCAO大鼠模型 ,设假手术组、模型组和模型 +补阳还五汤组 ,应用明胶酶谱法于再灌后 1、3、2 4、4 8h和 5d测定MMp.9蛋白表达。结果　①假手术组未检测到MMp.9;模型组和模型 +补阳还五汤组 2 4、4 8h可检测到活性MMp.9;4 8h模型组检测到MMp.9酶原。②模型组与模型 +补阳还五汤组同一时间点相比 ,在 2 4、4 8hMMp.9活性蛋白及酶原含量均下降 (p.p.9蛋白表达。

关键词：脑缺血再灌注损伤金属基质蛋白酶-9 明胶酶谱补阳还五汤干预作用血脑屏障

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p.ep.r.ion and identification of activity of anti-Hp.-6b/11E1 univer.al r.bozyme——r.1198 in vitr.

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Asian Jour.al of Andr.logy 1999年第4期1卷 195-201页

作者： De-Zhong LIU You-Xin JIN Hua HOU Yang-Zhong HUANG Guang-cai YANG Qian XU State Key Labor.tor. of Molecular.Biology, shanghai Institute of Biochemistr., Chinese Academy of Sciences, shanghai 200031, china department of Biochmeistr., Fir.t Militar. Medical university, Guangzhou 510515, china State Key Labor.tor. of Molecular.Biology Shanghai Institute of Biochemistry Chinese Academy of Sciences Shanghai 200031 China department of Biochemistr. First Military Medical University Guangzhou 510515 China

Aim: To study the p.ep.r.tion and cleavage activity of r.1196 dir.cted against Hp.-6bE1 and Hp.-11E1 (Hp.-6b/11E1) tr.nscr.p.s in vitr.. Methods: Hp.-6b/11E1 gene fr.gments wer. cloned into T-vector.under.the contr.l ofT7 p.omoter. ～(32)p.labeted Hp.-6b/11E1 tr.nscr.p.s as tar.et-r.As wer. tr.nscr.bed in vitr. and p.r.fied by ***1198 gene designed as a univer.al r.bozyme for.both Hp.-6b/11E1 tr.nscr.p.s was cloned into vector.p..5 between5'-cis-r. and 3'-cis-r.. a2p.labeled r.l198 tr.nscr.p. was gel-p.r.fied, incubated with tar.et-r.As at differ.nt condi-tions and autor.diogr.p.ed after.denatur.ng gel-electr.p.or.sis. r.sults: r.1198 was active at 37℃. The op.imaltemp.r.tur. was 50℃. For.Hp.-6bE1, k_m = 12.2 nmol/L, k_cat = 0.18 min～(-1); For.Hp.-11E1, k_m= 14.7 nmol/L,k_cat =0.14 min～(-1). All these r.vealed that the design of r.1198 was cor.ect. It could be a univer.al r.bozyme for.thetwo substr.tes--Hp.-6bE1 and Hp.-11E1 tr.nscr.p.s. Conclusion: r.1198 p.ep.r.d in vitr. p.ssesses the p.r.ectsp.cific catalytic cleavage activity. It leads to the exp.ctation that, in the futur., it will be p.ssible to develop.a newnucleic acid dr.g fr.m r.1198 which can efficiently inhibit the r.p.ication of Hp.-6b/11 DNA in vivo. (Asian J An-dr.l 1999 Dec; 1: 195-201)

关键词： r.bozyme Hp.-6b/11 genetic tr.nscr.p.ion cleavage identification of activity

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Chen's Theor.m with Small p.imes

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Chinese Annals of mathematics,Ser.es B 2011年第3期32卷 387-396页

作者： Yingjie LI yingchun cai College of Infor.ation Technology Shanghai Ocean University Shanghai 201306 China department of mathematics Tongji University Shanghai 200092 China

Let N be a sufficiently lar.e even *** p.denote a p.ime and p. denote an almost p.ime with at most two p.ime *** this p.p.r.it is p.oved that the equation N = p.+ p.(p.≤ N0.945) is solvable.

关键词： Chen's Theor.m Sieve method Mean value theor.m

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Concentr.tion and distr.bution of 17 or.anochlor.ne p.sticides (OCp.) in seawater.fr.m the Jap.n Sea nor.hwar. to the Ar.tic Ocean

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Science china Chemistr. 2010年第5期53卷 1033-1047页

作者： cai MingGang 1,2,QIU Canr.ng 1,SHEN Yuan 1,cai MingHong 3,HUANG ShuiYing 1,QIAN BiHua 1,SUN JiongHui 1 & LIU XiaoYan 1 1department of Oceanogr.p.y,College of Oceanogr.p.y and Envir.nmental Science,Xiamen university,Xiamen 361005,china 2State Key Labor.tor. of Mar.ne Envir.nmental Science,Xiamen university,Xiamen 361005,china 3State Oceanic Administr.tion Key Labor.tor. for.p.lar.Science,p.lar.r.sear.h Institute of china,shanghai 200136,china 1. department of Oceanogr.p.y College of Oceanography and Environmental Science Xiamen University Xiamen 361005 China2. State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China3. State Oceanic Administration Key Laboratory for Polar Science Polar Research Institute of China Shanghai 200136 China

Seventeen classic or.anochlor.ne p.sticides in sur.ace seawater.wer. studied in ter.s of their.comp.sition p.tter. as well as their.distr.bution p.tter. in the ar.as cover.ng the Jap.n Sea,Okhotsk Sea,Ber.ng Sea,Chukchi Sea and Ar.tic *** concentr.tions var.ed,but r.ughly two levels wer. seen with one r.nging between 0.1 and 1 ng L-1 for.most HCH isomer. and the other.lower.than 0.1 ng L-1 for.other.*** the 17 tar.et comp.unds,HCHs wer. dominant with a total concentr.tion p.r.entage gener.lly mor. than 50%,and a r.latively high concentr.tion p.r.entage of hep.achlor.and aldr.ne was also obser.ed at scatter.d *** histor.cal long-ter. tr.nd of sever.l tar.et chemicals in the five sea zones consider.d was discussed in comp.r.son with p.evious ***-sea zone comp.r.son was car.ied out for.individual chemicals by comp.r.ng the concentr.tion var.ation in all five sea zones.A higher.var.ation in concentr.tion was gener.lly found in the nor.her. sea zones,namely the Ber.ng Sea,Chukchi Sea and Ar.tic Ocean,for.most tar.et *** sum concentr.tion of the 17 tar.et chemicals disp.ayed a gener.l tr.nd of incr.asing nor.hwar. fr.m the Jap.n Sea to the Okhotsk Sea to the Ber.ng Sea to the Ar.tic *** latitudinal tr.nds wer. found for.-HCH andγ-HCH,and the r.ason of this differ.nce was discussed by consider.ng their.diver.ence of ther.odynamic p.op.r.ies,which could contr.bute to a slightly differ.nt fr.ctionation effect dur.ng their.nor.hwar. tr.nsp.r. dr.ven by atmosp.er.c long r.nge *** sour.e of the HCHs was identified by analyzing theγ-HCH/γ-HCH r.tio,which was less than 4 without excep.ion,indicating a comp.nent char.cter.stic featur.ng a mixtur. of technical HCHs and *** addition,the ver.ical distr.bution ofγ-HCH,γ-HCH and their.r.tio at station B80 was *** p.tter.s wer. found in the up.er.300 m while in layer. fr.m 300 m downwar. to 3500 m the p.tter.s wer. fair.y *** ver.ical p

关键词： or.anochlor.ne p.sticides Ar.tic Ocean solid-p.ase extr.ction solid-p.ase micr.extr.ction

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Synthesis and Str.ctur. of E-2-(2,4,6-tr. ter. butylp.enylp.osp.inyl)-2-〔N-(ter.-butyldimethylsilyl)-N-(p.chr.lop.enyl)〕amino-1-(2,4,6-tr. ter.-butyl-p.enyl)p.osp.acthylene

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Chinese Jour.al of Str.ctur.l Chemistr. 2000年第2期 86-90页

作者： ZHANG Li Xin p.NG Zhen * ZHOU Xi Geng cai r.i Fang HUANG Zu En (department of Chemistr., Fudan university, shanghai 200433) SUN Jie (shanghai Institute of Or.anic Chemistr., the Chinese Academy of Sciences,shanghai 200032) department of Chemistr. Fudan University Shanghai 200433 shanghai Institute of Or.anic Chemistr. the Chinese Academy of Sciences Shanghai 200032

The title comp.und E 2 (2,4,6 tr. ter. butylp.enylp.osp.inyl) 2 〔 N (ter.t butyldimethylsilyl) N (p.chr.lop.enyl)〕amino 1 (2,4,6 tr. ter. butyl p.enyl)p.osp.acthylene(C 49 H 78 NClp.2Si, M r.=806.65) was synthesized and char.cter.zed by X r.y diffr.ction analysis. The cr.stal is monoclinic, sp.ce gr.up.p. 1/n with a=10.319(3), b=23.309(5), c=21.41(1) , β=99.64(5)°, V=5078(3) 3, Z=4, D c =1.05g/cm 3, F(000)=1760, μ =1.92cm -1 . Due to the ster.c hindr.nce fr.m substituents ar.und p.osp.aethylene, the p.C bond length is significantly longer.than those obser.ed in its analogues. The values of the thr.e bond angles involved in the sp.2 hybr.dized car.on atom (C(1)) of the p.osp.aethylene ar. 133.4, 115.1 and 111.4° r.sp.ctively, deviating significantly fr.m the ideal value of 120°. In addition, although 5 sp.2 hybr.dized atoms (C(2), p.1), C(1), N and C(44)) ar. sequentially bonded in this molecule, no inter.ction is obser.ed among the r.maining p. or.its of these atoms on the str.ng.

关键词： or.anop.osp.ine p.osp.atheylene cr.stal str.ctur.

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Julolidinyl aza-BODIp.s as NIr.Ⅱ fluor.p.or.s for.the bioimaging of nanocar.ier.

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Acta p.ar.aceutica Sinica B 2024年第7期14卷 3155-3168页

作者： Chang Liu Yifan cai Zichen Zhang Yi Lu Quangang Zhu Haisheng He Zhongjian Chen Weili Zhao Wei Wu shanghai Skin Disease Hosp.tal Tongji University School of MedicineShanghai 200443China Key Labor.tor. of Smar. Dr.g Deliver. of MOE School of PharmacyFudan UniversityShanghai 201203China department of Medichinal Chemistr. School of PharmacyFudan UniversityShanghai 201203China

The aggr.gation-caused quenching(ACQ)r.tionale has been emp.oyed to imp.ove the fluor.scence imaging accur.cy of nanocar.ier. by p.ecluding fr.e p.obe-der.ved ***,its usefulness is under.ined by limited p.netr.tion and low sp.tiotemp.r.l r.solution of NIr.Ⅰ(700-900 nm)bioimaging owing to absor.0}ion and diffr.ction by biological tissues and tissue-der.ved *** study aimed to develop.ACQ-based NIr.Ⅱ(1000-1700 nm)p.obes to fur.her.imp.ove the imaging r.solution and *** str.tegy emp.oyed is to install highly p.anar.and electr.n-r.ch julolidine into the 3,5-p.sition of aza-BODIp. based on the lar.er.substituent *** newly develop.d p.obes disp.ayed r.mar.able p.otop.ysical p.op.r.ies,with intense absor.0}ion center.d at ap.r.ximately 850 nm and br.ght emission in the 950-1300 nm *** with the NIr.Ⅰ counter.0}r. p.,the NIr.Ⅱ p.obes demonstr.ted sup.r.or.water.sensitivity and quenching ***1 and ACQ6 exhibited mor. p.omising ACQ effects with absolute fluor.scence quenching at water.fr.ctions above 40% and higher.quenching stability with less than 2.0% fluor.scence r.illumination in p.asma after.24 h of *** calculations ver.fied that molecular.p.anar.ty is mor. imp.r.ant than hydr.p.obicity for.ACQ ***,in vivo and ex vivo r.illumination studies r.vealed less than 2.5% signal inter.er.nce fr.m p.equenched ACQ1,in contr.st to 15% for.p..

关键词： 3,5-Julolidinyl Aza-BODIp. Nanocar.ier. Near.infr.r.dⅡ Fluor.scence imaging Aggr.gation-caused quenching p.lymer.c micelles p.lymer.c nanop.r.icles

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腺病毒介导p.1基因转染抑制人血管平滑肌细胞增殖的研究

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复旦学报（医学版） 2003年第4期30卷 367-370,375页

作者：余波张学利王铁平李平蔡端复旦大学附属华山医院普外科上海200040 上海医学院生物化学与分子生物学系教研室上海200032

目的研究抗增殖性p.1基因转染对体外培养的人血管平滑肌细胞(HVSMC)的增殖抑制作用及其作用机制。方法通过腺病毒介导将外源性p.1基因转入HVSMC,通过免疫印迹法(Wester. blot)检测p.1基因的表达,原位末端标记法(TUNEL)、DNA片段梯度... 详细信息

目的研究抗增殖性p.1基因转染对体外培养的人血管平滑肌细胞(HVSMC)的增殖抑制作用及其作用机制。方法通过腺病毒介导将外源性p.1基因转入HVSMC,通过免疫印迹法(Wester. blot)检测p.1基因的表达,原位末端标记法(TUNEL)、DNA片段梯度分析及流式细胞分析(FCM)观察靶细胞的细胞周期变化及凋亡,利用酶联免疫光度仪分析靶细胞的增殖抑制情况。结果 Wester. blot结果显示p.1基因转染后的HVSMC可高水平表达p.1蛋白,而另两组无表达。感染后5 d,Adp.1组的细胞数为0.275 5±0.01(525 nm波长下吸光度A值),AdlacZ组为0.340 9±0.02,空白组为0.347 5±0.02,Adp.1组较另两组的差异有统计学意义(p.0.01)。TUNEL、DNA片段梯度分析及FCM检测均提示靶细胞发生了凋亡。FCM检测还发现明显的细胞周期阻滞现象,Adp.1组G0-G1期细胞比例为66.23％,S期细胞比例为10.18％,而另两组G0-G1期和S期细胞比例分别为:47.73％、30.56％和45.31％、32.34％。结论复制缺陷性腺病毒在体外可有效介导外源性p.1基因转染HVSMC,转染后的细胞除发生细胞周期阻滞外还出现明显的凋亡,其生长增殖受到抑制。

关键词：腺病毒介导 p.1基因人血管平滑肌细胞细胞增殖抑制作用基因治疗静脉移植血管成形术

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Cytochr.me p.50 2E1 genetic p.lymor.0}ism and gastr.c cancer.in Changle,Fujian p.ovince

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Wor.d Jour.al of Gastr.enter.logy 2001年第6期7卷 792-795页

作者： Lin cai~1 Shun-Zhang Yu~2 Zuo-Feng Zhang~3 1 department of Ep.demiology,Fujian Medical university,Fuzhou 350004,Fujian p.ovince,china2 department of Ep.demiology,shanghai Medical university,shanghai 200032,china3 department of Ep.demiology,UCLA School of p.blic Health,Los Angeles Califor.ia,USA department of Ep.demiology Fujian Medical University Fuzhou 350004 Fujian Province China. zjcailin@***

AIM: Genetic p.lymor.0}ism in enzymes of car.inogen metabolism has been found to have the influence on the suscep.ibility to cancer. Cytochr.me p.50 2E1 (CYp.E1) is consider.d to p.ay an imp.r.ant r.le in the metabolic activation of p.ocar.inogens such as N-nitr.soamines and low molecular.weight or.anic comp.unds. The p.r.0}se of this study is to deter.ine whether.CYp.50 2E1 p.lymor.0}isms ar. associated with r.sks of gastr.c cancer. METHODS: We conducted a p.p.lation based case-contr.l study in Changle county, Fujian p.ovince, a high-r.sk r.gion of gastr.c cancer.in china. Ninety-one incident gastr.c cancer.p.tients and ninety-four.healthy contr.ls wer. included in our.study. Datas including demogr.p.ic char.cter.stics, diet intake, and alcohol and tobacco consump.ion of individuals in our.study wer. comp.eted by a standar.ized ***-r.Lp.r.vealed thr.e genotyp.s:heter.zygote (C1/C2) and two homozygotes (C1/C1 and C2/C2) in CYp.E1. r.SULTS: The fr.quency of var.ant genotyp.s (C1/C2 and C2/C2) in gastr.c cancer.cases and contr.ls was 36.3% and 24.5%, r.sp.ctively. The r.r. homozygous C2/C2 genotyp. was found in 6 individuals in gastr.c cancer.gr.up.6.6%), wher.as ther. was only one in the contr.l gr.up.(1.1%). However. ther. was no statistically significant differ.nce between the two gr.up. (two-tailed Fisher.s exact test p.0.066). Individuals in gastr.c cancer.gr.up.wer. mor. likely to car.y genotyp. C1/C2 (odds r.tio, Or.1.50) and C2/C2 (Or.7.34) than individuals in contr.l gr.up.(chi(2) =4.597, for.tr.nd p.0.032). The fr.quencies of genotyp.s with the C2 allele (C1/C2 and C2/C2 genotyp.s) wer. comp.r.d with those of genotyp.s without C2 allele (C1/C1 genotyp.) among individuals in gastr.c cancer.gr.up.and contr.l gr.up.accor.ing to the p.tter. of gastr.c cancer.r.sk factor.. The r.sults show that individuals who exp.sed to these gastr.c cancer.r.sk factor. and car.y the C2 allele seemed to have a higher.r.sk of develop.ng gastr.c cancer. CONCLUSION: p.ly

关键词： p.lymor.0}ism, Genetic Aged Asian Continental Ancestr. Gr.up Case-Contr.l Studies china Cytochr.me p.450 CYp.E1 Female Gene Fr.quency Genetic p.edisp.sition to Disease Humans Male Middle Aged r.sear.h Sup.or., Non-U.S. Gov't Stomach Neop.asms

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The Kalman Filter.ng for.a class of Local Str.ngly Coup.ed Systems

The Kalman Filtering for a class of Local Strongly Coupled S...

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2011 Chinese Contr.l and Decision Confer.nce（CCDC）

作者： Yunze cai is with the department of Automation,shanghai Jiao Tong university,and Key Labor.tor. of System Contr.l and Infor.ation p.ocessing,Ministr. of Education of china,800 Dongchuan r..,shanghai 200240,p.r.china..Hua O.Wang is with the department of Mechanical Engineer.ng,Boston university,Boston,MA 02215,USA.Xiaoming Xu is with the department of Automation,shanghai Jiao Tong university,800 Dongchuan r..,shanghai 200240,p.r.china.,and with the university of shanghai for.Science and Technology,16 Jun Gong r..,shanghai 200093,p.r.china

This p.p.r.addr.sses a Kalman filter.ng p.oblem for.a class of local str.ngly coup.ed systems which ar. der.ved fr.m comp.ex systems with inter.communication or.constr.int between *** star. with,a multi-agent system is intr.duced and the communication matr.x is char.cter.zed by emp.oying r.ndom ser.es with Ber.oulli *** augmentation techniques and stochastic methods,the Kalman filter.ng algor.thm is *** exp.r.mental r.sults not only demonstr.te the effectiveness of the p.op.sed filter.ng method,but also show the inter.agent communication could imp.ove the tr.cing effect in the multi-agent collabor.tive systems.

关键词： Local str.ngly coup.ed systems Kalman filter Communication

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