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Aberration correction for deformable-mirror-based remote focusing enables high-accuracy whole-cell super-resolution imaging

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Photonics Research 2024年第4期12卷 821-832页

作者： WEI SHI yingchuan he JIANLIN WANG LULU ZHOU JIANWEI CheN LIWEI ZHOU ZEYU XI ZheN WANG KE FANG YIMING LI Department of Biomedical Engineering Southern University of Science and TechnologyShenzhen 518055China

Single-molecule localization microscopy(SMLM)enables three-dimensional(3D)investigation of nanoscale structures in biological samples,offering unique insights into their ***,traditional 3D super-resolution microscopy using high numerical aperture(NA)objectives is limited by imaging depth of field(DOF),restricting their practical application to relatively thin biological ***,we developed a unified solution for thick sample super-resolution imaging using a deformable mirror(DM)which served for fast remote focusing,optimized point spread function(PSF)engineering,and accurate aberration *** effectively correcting the system aberrations introduced during remote focusing and sample aberrations at different imaging depths,we achieved high-accuracy,large DOF imaging(~8μm)of the whole-cell organelles[i.e.,nuclear pore complex(NPC),microtubules,and mitochondria]with a nearly uniform resolution of approximately 35 nm across the entire cellular volume.

关键词： enable mirror resolution

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汕揭高速公路汕头段常规桥梁的抗震设计

公路工程 2013年第3期38卷 155-157页

作者：何颖川广东省公路勘察规划设计院股份有限公司广东广州510507

汕揭高速公路汕头段常规桥梁设计过程中,按《公路桥梁抗震设计细则》(JTG/T B02-01-2008)的计算方法进行抗震计算分析及设计,抗震分析结果表明抗震设计成为控制本项目设计的因素。

关键词：高速公路常规桥梁桥梁设计

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Does Obesity Affect Preoperative Thoracolumbar/Lumbar Curve Flexibility in Adolescent Idiopathic Scoliosis

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Does Obesity Affect Preoperative Thoracolumbar/Lumbar Curve ...

第三届中西医结合脊柱及相关疾病学术年会

作者： Li Ming,Zhao yingchuan,Wang Chuanfeng,he Shisheng Spine Surgery,the Affiliated Changhai Hospital of the Second Military Medical University,Shanghai,200433,China

*** orthopeadic diseases have lone been presupposed to be related with obesity,obesity has become a main concerned problem in Chinese adolescents in recent *** effect of obesity on main thoracolumbar/lumbar curve flex...

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Comparison of factors affecting curve flexibility in adolescent and adult idiopathic scoliosis.

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Comparison of factors affecting curve flexibility in adolesc...

第三届中西医结合脊柱及相关疾病学术年会

作者： Li Ming,Chen Ziqiang,Zhao yingchuan,he Shisheng,Wang Chuanfeng Spine Surgery,the Affiliated Changhai Hospital of the Second Military Medical University,Shanghai,200433,China

Study Design.A retrospective analysis of radiographs in adolescent and adult idiopathic scoliosis patients. *** determine and compare factors affecting curve flexibility in adolescent

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Correlation of Trunk Balance Immediately after Posterior Spinal Fusion with Postoperative Subjacent Disc Wedging in Adolescent Idiopathic Scoliosis

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Correlation of Trunk Balance Immediately after Posterior Spi...

第三届中西医结合脊柱及相关疾病学术年会

作者： Li Ming,Zhao yingchuan,he Shisheng,Zhu Xiaodong,Wang Chuanfeng Spine Surgery,the Affiliated Changhai Hospital of the Second Military Medical University,Shanghai,200433,China

Study Design.A retrospective radiographic study of surgically treated adolescent idiopathic scoliosis. *** investigate which radiographic parameter immediately after posterior

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The study of predictors of structural curves in Adolescent Idiopathic Scoliosis's minor curves

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The study of predictors of structural curves in Adolescent I...

第三届中西医结合脊柱及相关疾病学术年会

作者： Chen Ziqiang,Li Ming,Zhao yingchuan,he Shisheng,Wang Chuanfeng Spine Surgery,the Affiliated Changhai Hospital of the Second Military Medical University,Shanghai,200433,China

Study Design.A retrospective analysis of radiographs in adolescent scoliosis patients. *** determine the factors affecting a minor curve to be a structural one in adolescent idiopathic scoliosis patients.

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Characterization and molecular marker screening of a rice bacteria-resistant gene Xa-min(t)

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Progress in Natural Science:Materials International 2003年第10期 20-24页

作者： CheN Yan 1,2 , HU Jun 2, QIAN Wei 2, TIAN yingchuan 2 and he Chaozu 2*(1. Life Science and Technology Institute of Xi'an Jiaotong University, Xi'an 710049, China 2. Laboratory of Plant Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China)

To test the resistant spectrum of the Xa min(t) gene introgressed from Oryza minuta , thirty four isolates of different bacterial blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo), from 11 countries were used to inoculate the Xa min(t) introgression line 78 15. Four rice cultivars, IR24, C64 (IRBB21), Nipponbare and Zhonghua 11 were used as controls. The results showed that the Xa min(t) gene was broad spectrum and highly resistant to diverse Xoo isolates. The methods of bulk segregant analysis (BSA), randomly amplified polymorphic DNA (RAPD) and sequence characterized amplified regions (SCAR) were used to analyze F 2 individuals of the hybrid IR24×78 15 and molecular genetic markers linked to Xa min(t) gene were identified. A total of 800 arbitrary decamer oligonucleotide primers were used for RAPD analysis. Two RAPD markers, BE05 300 and BE06 1400 , produced by primers BE05 and BE06 respectively, were closely linked to the Xa min(t) gene. Based on the sequences of these two markers, sequence specific primers were designed and used to screen all F 2 plants. One RAPD marker, BE05 300 , was converted into a stable SCAR marker (ScBE05 300 ). Linkage analysis was carried out using markers ScBE05 300 and BE06 1400 on 948 and 719 F 2 individuals of the hybrid IR24×78 15. Our results indicate that the genetic distances from Xa min(t) to ScBE05 300 and BE06 1400 are 2.2 cM and 3.7 cM respectively on the same side. This study may facilitate the construction of the fine physical map of the Xa min(t) gene.

关键词： bacterial blight resistance gene Xa min(t) molecular marker.

Isolation and characterization of rice lesion mimic mutants from a T-DNA tagged population

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Progress in Natural Science:Materials International 2005年第1期 18-24页

作者： LI Shutian 1,2, PEI Zhongyou 1, LUO Lijuan 3, TIAN yingchuan 1 and he Chaozu 1* (1. National Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3. College of Agronomy, South China University of Tropical Agriculture, Danzhou 571737, China)

A rice (Oryza sativa L. ssp. japonica cv. Nipponbare) T-DNA tagged population consisting of about 7000 individual lines was generated and screened for rice lesion mimic mutants in the T 1 generation. Ten lines were found to develop spontaneous lesions in the absence of pathogen infection and displayed distinct lesion phenotypes. These mutants were tentatively designated as lm1—lm10 (for lesion mimic), respectively. Lesion formation of lm mutants was developmentally regulated, and all the mutants showed stunted growth and reduced fertility. Genetic analysis demonstrated that all the mutations were recessive, and five partially fertile mutants (lm4—lm8) were derived from different loci. Mimic lesions occurring on the leaves of lm mutants resulted from cell death as revealed by trypan blue staining. Six of them (lm3—lm8) exhibited enhanced resistance to five bacterial blight isolates, indicating their wide-spectrum resistance to this pathogen. These results imply that some lesion mimic mutations of rice might be involved in disease resistance signaling pathways, and that isolation of these mutated genes may be useful for elucidating molecular mechanisms of plant disease resistance. Among the mutants, only one mutant, lm6, was preliminarily shown to cosegregate with the inserted T-DNA in its T 1 generation, making it feasible to isolate the gene responsible for the phenotype of this mutant.

关键词： T-DNA tagging lesion mimic hypersensitive response Xanthomonas oryzae pv. Oryzae Oryza sativa.

Effect of UTRs from TMV-RNA on the expression of foreign gene in transgenic plants

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Chinese Science Bulletin 2000年第18期45卷 1681-1685页

作者： Hongmin Qin Hongnian Guo Yantao Jia Lihong Li yingchuan Tian Chinese Acad Sci Inst Microbiol Beijing 100080 Peoples R China

To investigate the effects of 5’and 3’ untranslated region (UTR) from tobacco mosaicvirus (TMV) on expression of foreign genes, four plant-expression vectors pBG437, pBG438, pBG440 and pBG440ANOS containing expression cassettes of GUS-NOS; Ω-GUS-NOS, Ω-GUS-3’ UTR-NOS and Ω-GUS-3’ UTR downstream of CaMV 35S promoter with double enhancer sequences respectively have been constructed. Results from a large number of transgenic tobacco plants show that the GUS activity of pBG440 transformed plants is the highest, being 5-fold that of pBG437 and 1.6-fold that of pBG438. Similar results have been obtained in transient expression by injection of Agrobacterium tumefaciens harbouring these constructs into N. benthamiana leaves. These results obtained at the whole plant level confirm the conclusion drawn from the transient expression studies in protoplast system that TMV- Ω fragment can be a translation enhancer and the 3’ UTR has a coordinate effect with ft fragment to enhance foreign gene expression, but

关键词： UTR of TMV-RNA β-giucuronidase gene transgenic tobacco plants.

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