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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第10期14卷 760-766页

作者： Li-yan DAI qiu-long SHI Jing ZHANG Xiao-zhong WANG ying-qi chen Department of Chemical and Biological Engineering Zhejiang University

Solubility of 2-amino-6-chloropurine in Mitsunobu solvents could be significantly improved after its exocyclic amino group is protected via *** bis-Boc protected 2-amino-6-chloropurine also shows excellent activity and N9 selectivity in the coupling with various alcohols by a Mitsunobu ***,a new practical and efficient method is established for the synthesis of penciclovir(PCV) from bis-Boc-2-amino-6-chloropurine 9 and the side chain of 5-(2-hydroxyethyl)-2,2-dimethyl-1,3-dioxane 5—the latter being a more easily prepared cyclic precursor of the diacetate side chain used in the conventional *** coupling of 9 with 5 proceeded regioselectively at a N9 position of purine derivative for a good yield under Mitsunobu conditions.

关键词： 2-amino-6-chloropurine Mitsunobu reaction bis-Boc protection Penciclovir(PCV)

Dicyclopentyl azodicarboxylate(DCpAD):A new alternative azo-reagent for the Mitsunobu reaction

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Chinese Chemical Letters 2011年第9期22卷 1047-1050页

作者： Jian Hai Yang Li Yan Dai Xiao Zhong Wang ying qi chen Department of Chemical and Biological Engineering Zhejiang University Hangzhou 310027 China

Dicyclopentyl azodicarboxylate is introduced as a new azo-reagent which can be conveniently prepared in two steps and be used in the Mitsunobu reaction. Though there are no distinct difference of reactivity between DC... 详细信息

关键词： Dicyclopentyl azodicarboxylatc Mitsunobu reaction Azo-reagent

DNA-dependent activator of interferon-regulatory factors inhibits hepatitis B virus replication

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World Journal of Gastroenterology 2012年第22期18卷 2850-2858页

作者： qi-ying chen ying-Hui Liu Jian-Hua Li Ze-Kun Wang Jiang-Xia Liu Zheng-Hong Yuan Key Laboratory of Medical Molecular Virology Ministry of Education and Ministry of Health Shanghai Medical College Fudan University Shanghai 200032 China Institutes of Biomedical Sciences Fudan University Shanghai 200032 China

AIM： To investigate whether DNA-dependent activator of interferon-regulatory factors （DAI） inhibits hepatitis B virus （HBV） replication and what the mechanism is. METHODS： After the human hepatoma cell line Huh7 was cotransfected with DAI and HBV expressing plas- mid, viral protein （HBV surface antigen and HBV e an- tigen） secretion was detected by enzyme-linked immu- nosorbent assay, and HBV RNA was analyzed by real- time polymerase chain reaction and Northern blotting, and viral DNA replicative intermediates were examined by Southern blotting. Interferon regulatory factor 3 （IRF3） phosphorylation and nuclear translocation were analyzed via Western blotting and immunofluorescence staining respectively. Nuclear factor-KB （NF-KB） activity induced by DAI was detected by immunofluorescence staining of P65 and dual luciferase reporter assay. Tran- swell co-culture experiment was performed in order to investigate whether the antiviral effects of DAI were dependent on the secreted cytokines. RESULTS： Viral protein secretion was significantly re- duced by 57% （P 〈 0.05）, and the level of total HBV RNA was reduced by 67% （P 〈 0.05）. The viral core particle-associated DNA was also dramatically down- regulated in DAI-expressing Huh7 cells. Analysis of involved signaling pathways revealed that activation of NF-KB signaling was essential for DAI to elicit antivi- ral response in Huh7 cells. When the NF-KB signaling pathway was blocked by a NF-KB signaling suppressor （I~：B^-SR）, the anti-HBV activity of DAI was remarkably abrogated. The inhibitory effect of DAI was indepen- dent of IRF3 signaling and secreted cytokines. CONCLUSION： This study demonstrates that DAI can inhibit HBV replication and the inhibitory effect is asso- ciated with activation of NF-KB but independent of IRF3 and secreted cytokines.

关键词： DNA-dependent activator of interferon regu-latory factor Antiviral activity Hepatitis B virus Nuclearfactor-~B Interferon regulatory factor-3

高效的磁性NiCo2O4纳米粒子催化脑文格尔缩合反应的研究（英文）

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2020年第1期21卷 74-84页

作者： Yang-yang FANG Xiao-zhong WANG ying-qi chen Li-yan DAI Zhejiang Provincial Key Laboratory of Advanced Chemical Engineering Manufacture Technology College of Chemical and Biological Engineering Zhejiang University

目的:脑文格尔缩合反应已被广泛应用于精细化学品、药物、香料和化妆品等领域,是重要的有机合成反应之一。传统的缩合反应存在催化剂稳定性差、分离难、催化活性低等问题。本文旨在探讨NiCo2O4的制备方法与结构的关系和催化剂用于脑文... 详细信息

目的:脑文格尔缩合反应已被广泛应用于精细化学品、药物、香料和化妆品等领域,是重要的有机合成反应之一。传统的缩合反应存在催化剂稳定性差、分离难、催化活性低等问题。本文旨在探讨NiCo2O4的制备方法与结构的关系和催化剂用于脑文格尔缩合反应的条件对性能的影响。创新点:1.通过采用不同的煅烧温度,确定合成NiCo2O4尖晶石催化剂的最适温度,以减少NiO的生成;2.优化反应条件,使苯甲醛和丙二腈的缩合反应收率达到99%;3.获得的NiCo2O4催化剂的稳定性很高,在循环20次后催化性能仍保持不变。方法:1.通过X射线单晶衍射和傅里叶红外光谱分析,推断在不同煅烧温度下合成的催化剂的组成,并确定最佳煅烧温度(图2和3);2.对NiCo2O4尖晶石催化剂进行透射电镜、二氧化碳/氨气程序升温脱附和氮气吸脱附表征,探究其微观结构与性能之间的关系(图4~8);3.通过苯甲醛和丙二腈的脑文格尔缩合反应,验证催化剂的活性。结论:1.成功合成出高效的磁性NiCo2O4纳米颗粒;2.以脑文格尔缩合反应作为探针反应,发现NiCo2O4具有高催化性能,并且其反应转化率和收率高达99%;3.催化剂具有磁性,易于回收;4.在循环20次后,反应活性不变,说明催化剂具有高稳定性。

关键词： NiCo2O4催化剂尖晶石脑文格尔缩合反应非均相催化

(3S)-1-[(R)-2-羟基-1-苯乙基]-3-甲基-2-哌啶酮的合成（英文）

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2016年第2期17卷 163-170页

作者： Xiao-zhong WANG Xia WANG ying-qi chen Li-yan DAI Xing-cong LI College of Chemical and Biological Engineering Zhejiang University National Centers for Natural Products Research Research Institute of Pharmaceutical Sciences The University of Mississippi

目的:探索合成(3S)-1-[(R)-2-羟基-1-苯乙基]-3-甲基-2-哌啶酮的新方法。创新点:以常规化工原料D-苯苷氨醇为主要原料,在比较温和的条件下合成重要的药物中间体(3S)-1-[(R)-2-羟基-1-苯乙基]-3-甲基-2-哌啶酮及其同系物。该方法中的甲... 详细信息

目的:探索合成(3S)-1-[(R)-2-羟基-1-苯乙基]-3-甲基-2-哌啶酮的新方法。创新点:以常规化工原料D-苯苷氨醇为主要原料,在比较温和的条件下合成重要的药物中间体(3S)-1-[(R)-2-羟基-1-苯乙基]-3-甲基-2-哌啶酮及其同系物。该方法中的甲基化步骤较常规甲基化步骤减少1当量s-Bu Li的用量,更环保和安全。方法:利用D-苯苷氨醇的空间位阻作用,在六元环内酰胺中引入具有特定光学纯度的手性甲基。在甲基化过程中,用叔丁基二甲基氯硅烷对羟基进行保护,以减少仲丁基锂的用量。结论:以工业易得的δ-戊内酯及D-苯苷氨醇为初始原料,探索合成3-甲基-2-哌啶酮类物质的新方法。新方法中对仲丁基锂的消耗量与常规方法有所不同。当羟基受保护时,甲基化1当量六元环内酰胺(化合物7)消耗1.5当量而非2.5当量仲丁基锂,甲基化产物脱掉醇羟基保护基,得到(3S)-1-[(R)-2-羟基-1-苯乙基]-3-甲基-2-哌啶酮(化合物1)及其非对映异构体(3R)-1-[(R)-2-羟基-1-苯乙基]-3-甲基-2-哌啶酮(化合物8),二者摩尔比为1:2.5。通过重结晶或柱层析的方法可对二者进行完全分离。

关键词：哌啶酮生物碱不对称合成 D-苯甘氨醇醇羟基保护基

Efficient derivation of functional hepatocytes from mouse induced pluripotent stem cells by a combination of cytokines and sodium butyrate

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Chinese Medical Journal 2011年第22期124卷 3786-3793页

作者： ZHANG qi YANG Yang ZHANG Jian WANG Guo-ying LIU Wei qiU Dong-bo HEI Zi-qing ying qi-long chen Gui-hua Guangdong Provincial Key Laboratory of Liver Disease Guangzhou Guangdong 510630 China

Background Hepatocyte transplantation has been proposed as an alternative to whole-organ transplantation to support many forms of hepatic insufficiency. Unfortunately, the lack of donor livers makes it difficult to obtain enough viable human hepatocytes for hepatocyte-based therapies. Therefore, it is urgent to find new ways to provide ample hepatocytes. Induced pluripotent stem （iPS） cells, a breakthrough in stem cell research, may terminate these hinders for cell transplantation. For the promise of iPS cells to be realized in liver diseases, it is necessary to determine if and how efficient they can be differentiated into functional hepatocytes. Methods In this study, we directly compared the hepatic-differentiation capacity of mouse iPS cells and embryonic stem （ES） cells with three different induction approaches： conditions via embryonic body （EB） formation plus cytokines, conditions by combination of dimethyl sulfoxide and sodium butyrate and chemically defined, serum free monolayer conditions. Among these three induction conditions, more homogenous populations can be promoted under chemically defined, serum free conditions. The cells generated under these conditions exhibited hepatic functions in vitro, including glycogen storage, indocynine green （ICG） uptake and release as well as urea secretion. Although efficient hepatocytes differentiation from mouse iPS cells were observed, mouse iPS cells showed relatively lower hepatic induction efficiency compared with mouse ES cells. Results Mouse iPS cells would be efficiently differentiated into functional hepatocytes in vitro, which may be helpful in facilitating the development of hepatocytes for transplantation and for research on drug discovery. Conclusion We demonstrate that mouse iPS cells retain full potential for fetal liver development and describe procedures that facilitates the efficient generation of highly differentiated human hepatocyte-like cells from iPS cells in vitro.

关键词： induced pluripotent stem cells hepatocytes differentiation," embryonic stem cells cytokines

Atmospheric reductive catalytic fractionation of lignocellulose integrated with one-pot catalytic conversion of carbohydrate yielding valuable lignin monomers and platform chemicals from corn straw

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Green Energy & Environment 2025年第1期10卷 161-172页

作者： Meng-ying Liu Zhe-Hui Zhang Xue-qi Wang qian Sun chen Zhang Yu Li Zhuohua Sun Katalin Barta Feng Peng Tong-qi Yuan State Key Laboratory of Efficient Production of Forest Resources Beijing Forestry UniversityBeijing100083China Beijing Key Laboratory of Lignocellulosic Chemistry Beijing Forestry UniversityBeijing100083China Department of Chemistry Organic and Bioorganic ChemistryUniversity of GrazHeinrichstrasse 28/II8010 GrazAustria

Developing a cost-effective and environmentally friendly process for the production of valuable chemicals from abundant herbal biomass receives great attentions in recent ***,taking advantage of the“lignin first”strategy,corn straw is converted to valuable chemicals including lignin monomers,furfural and 5-methoxymethylfurfural via a two steps *** key of this research lies in the development of a green and low-cost catalytic process utilizing magnetic Raney Ni catalyst and high boiling point ethylene *** utilization of neat ethylene glycol as the sole slovent under atmospheric conditions obviates the need for additional additives,thereby facilitating the entire process to be conducted in glass flasks and rendering it highly convenient for scaling *** the initial step,depolymerization of corn straw lignin resulted in a monomer yield of 18.1 wt%.Subsequently,in a dimethyl carbonate system,the carbohydrate component underwent complete conversion in a one-pot process,yielding furfural and 5-methoxymethylfurfural as the primary products with an impressive yield of 47.7%.

关键词： Atmospheric reductive catalytic fractionation Corn straw Ethylene glycol Raney Ni 5-Methoxymethylfurfural

Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

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Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui Qu Xiangbing Wu Wei Wu ying Wang Yan Sun Lingxiao Deng Melissa Walker chen chen Heqiao Dai qi Han ying Ding Yongzhi Xia George Smith Rui Li Nai-Kui Liu Xiao-Ming Xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Hand Surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals Pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA Department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

Predictive Value of Serum Creatinine, Blood Urea Nitrogen,Uric Acid, and β2-Microglobulin in the Evaluation of Acute Kidney Injury after Orthotopic Liver Transplantation

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Chinese Medical Journal 2018年第9期131卷 1059-1066页

作者： Hai-Yang Lu Xin-Yu Ning ying-qi chen Shu-Jun Han Ping Chi Sai-Nan Zhu Yun Yue Department of Anaesthesiology Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Department of Anaesthesiology Beijing You An Hospital Capital Medical University Beijing 100069 China Department of Anaesthesiology Beijing Tsinghua Changgung Hospital School of Clinical Medicine Tsinghua University Beijing 102218 China Department of Anaesthesiology The General Hospital of the Chinese People's Armed Police Forces Beijing 100039 China Department of Biostatistics Peking University First Hospital Beijing 100034 China

Background：As a major complication after orthotopic liver transplantation （OLT）,the occurrence of acute kidney injury （AKI） is frequently defined by serum creatinine （Cr）;however,the accuracy of commonly used blood urea nitrogen （BUN）,uric acid （UA）,and β2-microglobulin （β2-MG） remains to be *** retrospective study compared the accuracy of these parameters for post-OLT AKI ***：Patients who underwent OLT in three centers between July 2003 and December 2013 were *** postoperative AKI group was diagnosed by the Kidney Disease Improving Global Outcomes （KDIGO） criteria and classified by *** data were analyzed using the t-test or Wilcoxon rank-sum test;enumerated data were analyzed using the Chi-square test or Fisher＇s exact *** reliability and predictive accuracy were evaluated using receiver operating characteristic （ROC） curve ***：This study excluded 976 cases and analyzed 697 patients （578 men and 1 1 9 women）;the post-OLT AKI incidence was *** with the no-AKI group,the AKI group showed very significant differences in Model for End-stage Liver Disease score （14.74 ± 9.91 vs.11.07 ± 9.54,Z =5.404;P ＜ 0.001）,hepatic encephalopathy （45 [15.8％] vs.30 [7.3％],x2 =12.699;P ＜ 0.001）,hemofiltration （28 [9.8％] vs.0 [0.0％],x2 =42.171;P ＜ 0.001）,and 28-day mortality （23 [8.1％] vs.9 [2.2％],x2 13.323;P ＜0.001）.Moreover,mean values of Cr,BUN,UA,and β2-MG in the AKI group differed significantly at postoperative days 1,3,and 7 （all P ＜0.001）.ROC curve area was 0.847 of Cr for the detection of AKI Stage 1 （sensitivity 80.1％,specificity 75.7％,cutoffvalue 88.23 μmol/L）,0.916 for Stage 2 （sensitivity 87.6％,specificity 82.6％,cutoff value 99.9 μmol/L）,and 0.972 for Stage 3 （sensitivity 94.1％,specificity 88.2％,cutoff value 122.90 μmol/L）.Conclusion：The sensitivity and specificity of serum Cr might be a high-value indicator for the diagnosis and grading of post-OLT AKI.

关键词： Acute Kidney Injury Blood Urea Nitrogen Creatinine Liver Transplantation Uric Acid β2-Microglobulin

Ammonia pyrolysis oxidation excited by nanosecond pulsed discharge:Global/fluid models hybrid solution

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Journal of Energy Chemistry 2025年第1期100卷 385-396页

作者： Mingming Zhang Hanyu Wu qi chen Jiaxin Yang Bonan Liu School of Mechanical Electronic and Control Engineering Beijing Jiaotong University China University of Petroleum

The kinetic characteristics of plasma-assisted oxidative pyrolysis of ammonia are studied by using the global/fluid models hybrid solution ***,the stable products of plasma-assisted oxidative pyrolysis of ammonia are *** results show that the consumption of NH3/O2and the production of N2/H2change linearly with the increase of voltage,which indicates the decoupling of nonequilibrium molecular excitation and oxidative pyrolysis of ammonia at low ***,the detailed reaction kinetics mechanism of ammonia oxidative pyrolysis stimulated by a nanosecond pulse voltage at low pressure and room temperature is *** on the reaction path analysis,the simplified mechanism is *** detailed and simplified mechanism simulation results are compared with experimental data to verify the accuracy of the simplified ***,based on the simplified mechanism,the fluid model of ammonia oxidative pyrolysis stimulated by the nanosecond pulse plasma is established to study the pre-sheath/sheath behavior and the resultant consumption and formation of key *** results show that the generation,development,and propagation of the pre-sheath have a great influence on the formation and consumption of *** consumption of NH3by the cathode pre-sheath is greater than that by the anode pre-sheath,but the opposite is true for OH and O（1S）.However,within the sheath,almost all reactions do not ***,by changing the parameters of nanosecond pulse power supply voltage,it is found that the electron number density,electron current density,and applied peak voltages are not the direct reasons for the structural changes of the sheath and ***,the discharge interval has little effect on the sheath structure and gas mixture *** research results of this paper not only help to understand the kinetic promotion of non-equilibrium excitation in the process of oxidative pyrolysis but also help to explore th

关键词： Nanosecond pulsed discharge Ammonia Oxidative pyrolysis Fluid model Global model