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TNF-α通过CXCL10/CXCR3信号通路促进结肠癌细胞上皮间质化的相关分子机制

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分子诊断与治疗杂志 2024年第2期16卷 326-330页

作者：李艳萌徐德龙夏向峰吴西彩李园园律洁日照市人民医院检验科山东日照276800 日照市中医医院小儿外科山东日照276800 日照市精神卫生中心影像科山东日照276800

目的探讨TNF-α通过调控结肠癌SW480细胞CXCL10/CXCR3轴的表达影响上皮间质化(EMT)及其分子机制。方法培养人结肠癌SW480细胞,分别通过TNF-α、siRNA-CXCR3处理细胞,将细胞分为对照组(NC组)、TNF-α刺激组(TNF-α组)及TNF-α刺激+siRN... 详细信息

目的探讨TNF-α通过调控结肠癌SW480细胞CXCL10/CXCR3轴的表达影响上皮间质化(EMT)及其分子机制。方法培养人结肠癌SW480细胞,分别通过TNF-α、siRNA-CXCR3处理细胞,将细胞分为对照组(NC组)、TNF-α刺激组(TNF-α组)及TNF-α刺激+siRNA-CXCR3沉默组(TNF-α+si-CXCR3组)。通过Real-time PCR检测各组细胞CXCL10、CXCR3及上皮间质化相关基因的mRNA表达量;Western Blot检测各组细胞CXCL10/CXCR3通路蛋白及上皮间质化相关蛋白表达量的影响;免疫组化检测细胞中上EMT上皮标志物上皮细胞钙粘蛋白(E-cad)与EMT间质标志物波形蛋白(Vimentin)的变化情况;划痕实验检测各组细胞的迁移能力;Transwell实验检测各组细胞的侵袭能力。结果 TNF-ɑ组细胞中通路基因CXCL10、CXCR3的m RNA水平高于NC组,差异有统计学意义(P<0.05),TNF-α+si-CXCR3组细胞中CXCR3水平低于TNF-α组,差异有统计学意义(P<0.05);TNF-ɑ组细胞中CXCL10、CXCR3、Vimentin与Fibronectin的蛋白表达量高于NC组,E-cad的蛋白表达量低于NC组,差异有统计学意义;TNF-α+si-CXCR3组细胞CXCR3、Vimentin与Fibronectin的蛋白表达量低于TNF-ɑ组,E-cad的蛋白水平高于TNF-ɑ组;TNF-ɑ组中Vimentin的表达量高于NC组,E-cad的表达量低于NC组,差异有统计学意义(P<0.05),TNF-α+siCXCR3组中Vimentin的表达量低于TNF-ɑ组,差异有统计学意义(P<0.05),E-cad的表达量高于TNF-ɑ组;TNF-ɑ组迁移能力高于NC组,差异有统计学意义(P<0.05),TNF-α+si-CXCR3组细胞迁移能力低于TNF-ɑ组,差异有统计学意义(P<0.05)。TNF-ɑ组细胞的侵袭能力高于NC组,差异有统计学意义(P<0.05),TNF-α+si-CXCR3组细胞侵袭能力低于TNF-ɑ组,差异有统计学意义(P<0.05)。结论 TNF-α能够诱导结肠癌SW480细胞发生上皮间质化,并促进其迁移、侵袭水平,这一过程可能与激活CXCL10/CXCR3信号通路有关。

关键词：结肠癌 SW480 CXCL10/CXCR3 TNF-α 上皮间质化

Performance of a c-and a-Cut Ho:YAP Laser at Room Temperature

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Chinese Physics Letters 2012年第3期29卷 114-116页

作者： SHEN ying-jie YAO Bao-Quan DAI Tong-Yu LI-Gang DUAN xiao-Ming JU You-Lun WANG Yue-Zhu National Key Laboratory of Tunable Laser Technology Harbin Institute of TechnologyHarbin 150001

Continuous-wave operation of room-temperature c-and a-cut Ho:YAP lasers resonantly end-pumped by a diodepumped Tm:YLF laser at 1.91μm are comparatively *** maximum output powers of 7.8 W at 2103nm and 6.09 W at 2130 nm for c-cut Ho:YAP crystal are *** maximum output power of 8.78 W at 2119nm for a-cut Ho:YAlO_(3)crystal is *** maximum slope efficiency in terms of absorbed pump power approximately reaches 57.8%for c-oriented crystal and about 52.7%for a-oriented crystal.

关键词： Tm:YLF pumped pump

下缘小切口“V”形辅助皮瓣结合打包固定法治疗腋臭的临床疗效观察

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河西学院学报 2024年第5期40卷 27-30页

作者：王晓琼牛生浩张杰常莉陈静刘志侠河西学院附属张掖人民医院甘肃张掖734000

目的:探索腋窝下缘小切口“V”形辅助皮瓣剪除顶泌汗腺结合打包固定法治疗腋臭的临床应用及疗效。方法:将2021年8月-2023年8月笔者医院整形美容科手术治疗的80例腋臭患者作为研究对象,采用肿胀麻醉下腋窝下缘小切口“V”形辅助皮瓣剪除... 详细信息

目的:探索腋窝下缘小切口“V”形辅助皮瓣剪除顶泌汗腺结合打包固定法治疗腋臭的临床应用及疗效。方法:将2021年8月-2023年8月笔者医院整形美容科手术治疗的80例腋臭患者作为研究对象,采用肿胀麻醉下腋窝下缘小切口“V”形辅助皮瓣剪除顶泌汗腺结合打包固定法治疗,对手术治疗效果随访观察。结果:80例患者均一次性治愈,手术治疗总有效率100%,术后发生并发症者3例,2例于术后第3天出现双侧皮下血肿,另1例拆包时发现双侧切口局部皮肤坏死,均为男性,并发症发生率为3.75%,术后进行3-24月随访,无失访病例,无复发病例,随访患者均无明显瘢痕增生。结论:肿胀麻醉下腋窝下缘小切口“V”形辅助皮瓣剪除顶泌汗腺结合打包固定法治疗腋臭,能避免皮下血肿、皮瓣坏死及术后复发这些常见并发症,治疗效果好,手术方法简单,且切口瘢痕隐蔽,皮肤色泽度基本与正常皮肤一致,达到患者治疗与美观的要求,值得推广。

关键词：腋臭下缘小切口“V”形辅助皮瓣打包固定法

索拉非尼在碘难治性分化型甲状腺癌中耐药机制现状

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中国临床药理学杂志 2024年第13期40卷 1986-1990页

作者：张恩涛诸昊楠温正则张涔慧赵义欢茅颖杰吴俊朴金煜城金欣绍兴文理学院医学院浙江绍兴312000

大多数分化型甲状腺癌症患者在放射性碘-131治疗后预后良好,但仍有少数患者对放射性碘治疗不敏感,甚至依然出现疾病进展。目前,一些可以提高放射性碘难治性分化型甲状腺癌症患者无进展生存率的靶向药物,如索拉非尼和乐伐替尼,已被批准... 详细信息

大多数分化型甲状腺癌症患者在放射性碘-131治疗后预后良好,但仍有少数患者对放射性碘治疗不敏感,甚至依然出现疾病进展。目前,一些可以提高放射性碘难治性分化型甲状腺癌症患者无进展生存率的靶向药物,如索拉非尼和乐伐替尼,已被批准用于治疗放射性碘难治性分化型癌症(RAIR-DTC)。然而,由于原发性和继发性耐药的存在,药物对这些患者的疗效并不理想。本综述就丝裂原活化蛋白激酶(MAPK)和磷脂酰肌醇3-激酶(PI3K)通路介绍索拉非尼继发性耐药机制并提出相关治疗策略,以期为索拉非尼类似药物耐药机制与放射性碘难治性分化型甲状腺癌的有效治疗提供参考依据。

关键词：放射性碘难治性分化型甲状腺癌索拉非尼耐药机制协同作用丝裂原活化蛋白激酶信号通路磷脂酰肌醇3-激酶信号通路

Proteomic analysis of the immune response of the silkworm infected by Escherichia coli and Bacillus bombyseptieus

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Insect Science 2012年第5期19卷 559-569页

作者： xiao-Wu Zhong Ping Zhao Yong Zou Hong-Yi Nie Qi-ying Yi Qing-You xia Zhong-Huai xiang The Key Sericultural Laboratory of Agriculture Ministry Southwest University Chongqing China

he silkworm, Bombyx mori, is an economically important insect with a 5 000-year history of domestication. During evolution, the silkworm has developed highly effective defenses against invasion and parasitization by microorganisms. In this study, two microorganisms Escherichia coli and Bacillus bombyseptieus were orally infected to silk- worm larvae. After infection with E. coli and B. bombyseptieus for 24 h, we investigated the polypeptide changes in the hemolymph, midgut and integument using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time of flight mass spec- trometry. Forty-seven differentially expressed proteins were identified in these tissues. They belonged to a variety of functional classes, including immune proteins, metabolic proteins and structural proteins. Compared with controls, E. coli-infected silkworms showed 21 up- regulated proteins, 25 down-regulated proteins and lost one protein. After infection with B. bombyseptieus, silkworms showed 15 up-regulated proteins, 27 down-regulated pro- teins, lost three proteins and retained two proteins unchanged. We speculate that all these proteins may play a role in the silkworm immune response, although it is unclear why and how the two kinds of bacteria can so markedly alter expression of these proteins. These re- sults offer valuable insights for measuring the proteomic responses of the silkworm innate immune mechanism.

关键词： Bacillus bombyseptieus Escherichia coli immune response proteomics silkworm

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Ti_(2)AlNb合金的嵌入原子势

物理学报 2022年第20期71卷 65-74页

作者：刘杰刘艳侠辽宁大学物理学院沈阳110036

分子动力学模拟是一种行之有效的计算机模拟方法;然而,由于缺少合适的多元合金原子间势,因而限制了分子动力学模拟的应用.多元合金原子间势的开发一直具有挑战性.本文在嵌入原子势模型的框架下,提出一种适用于三元有序合金的原子间势构... 详细信息

分子动力学模拟是一种行之有效的计算机模拟方法;然而,由于缺少合适的多元合金原子间势,因而限制了分子动力学模拟的应用.多元合金原子间势的开发一直具有挑战性.本文在嵌入原子势模型的框架下,提出一种适用于三元有序合金的原子间势构建方法,并开发了适用于原子尺度力学行为模拟的Ti_(2)AlNb合金新型原子间势.该势能够很好地再现B2-Ti_(2)AlNb的弹性常数、未弛豫的空位形成能、置换原子形成能、换位原子形成能、表面能和三种有序构型(B2相、D0_(19)相、O相)在不同体积下的内聚能.为了进一步检验势函数,计算了B2相的E-V曲线,结果与Rose曲线符合得很好;利用分子动力学模拟研究了B2相的熔化转变过程,结果大致反映了实验情况.本文的工作一方面为开发多元合金原子间势提供一种途径,另一方面为模拟计算Ti_(2)AlNb合金的工作者提供一种选择.

关键词：嵌入原子势 Ti_(2)AlNb合金缺陷

Re-epithelialization of the prostatic urethra after two-micron laser resection of the prostate

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Asian Journal of Andrology 2016年第1期18卷 151-153页

作者： Guang-Heng Luo Hong Liu Lei Luo Jun Liu Xiu-Shu Yang Yan-Dong Wang Zhao-Lin Sun Shu-jie xia Department of Urology Surgery Guizhou Province People's Hospital Guiyang China Department of Urology Surgery Institute of Urology Shanghai JiaoTong University Shanghai China Department of Physiology North Sichuan Medical College Nanchong China Department of Research and Education Guizhou Province People's Hospital Guiyang China

Dear Editor, Benign prostatic hyperplasia （BPH） is the most common genitourinary tract disease in elderly *** two-micron laser combines the advantages of efficient resection and rapid vaporization, resulting in high-performance resection and excellent hemostasis in resection of the prostate adenoma, which makes it an effective and promising modality in BPH treatment. However, severe edema and necrosis of the remaining prostatic tissue after vaporesection of the prostatic adenoma may cause severe irritative and/or obstructive symptoms and may even result in the need for a second operation. Rapid wound re-epithelialization in the prostatic urethra can, however, reduce the incidence of postoperative complications after transurethral resection of the prostate （TURP）.

关键词： STEM-CELLS

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Biomedical and Environmental Sciences 2013年第6期26卷 492-495页

作者： YIN Jian Hai YUAN Zhong ying CAI Hui xia SHEN Yu Juan JIANG Yan Yan ZHANG Jing WANG Yan Juan CAO Jian Ping National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention Key Laboratory of Parasite and Vector Biology MOHChina WHO Collaborating Center for Malaria Schistosomiasis and Filariasis Qinghai Institue for Endemic Disease Prevention and Control

Objective Pigs, as hosts of zoonotic Cryptosporidium species/genotypes, are domestic animals with public health significance. The present study was to characterize the infection rate and species/genotype of Cryptosporidium in pre-weaned and post-weaned pigs from Shanghai and Shaoxing, China. Methods A total of 208 fecal samples （42 from pre-weaned piglets, and 166 from post-weaned pigs） were examined by nested PCR of the 185 rRNA gene and analyzed by phylogenetic DNA fragment sequencing of secondary PCR products. Results Infection was detected in 79 samples （19/42 pre-weaned piglets, and 60/166 post-weaned pigs）. C. suis （14/79） and Cryptosporidium pig genotype II （65/79） were identified; piglets were more susceptible to the former （13/14） and post-weaned pigs to the latter （59/65）. Conclusion Infection of Cryptosporidium spp. in pigs was age-specific; piglets were more susceptible to C. suis while pigs were more susceptible to Cryptosporidium pig genotype II. These findings combined with the isolation of the two Cryptosporidium from water suggest that pigs may be a source of zoonotic Cryptosporidium water pollution. Improvements in pig feeding practices, sewage discharge, feces disposal and field worker protection are therefore important to prevent potential public health problems.

关键词： Cryptosporidium suis Cryptosporidium pig genotype II Age-specificity Pigs China

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补骨脂化学成分研究进展

中国现代中药 2024年第4期26卷 733-748页

作者：杨秀伟许青霞吕倩和子超徐嵬张英涛北京大学药学院天然药物学系/天然药物及仿生药物全国重点实验室北京100191

补骨脂是豆科植物Psoralea corylifolia L.的干燥成熟果实,作为一种传统的中药,其在我国已有几千年的临床应用历史,广泛地用于心血管疾病、肾炎、骨质疏松和癌症等的治疗。植物化学研究显示,香豆素类、杂萜类和黄酮类是其主要化学成分... 详细信息

补骨脂是豆科植物Psoralea corylifolia L.的干燥成熟果实,作为一种传统的中药,其在我国已有几千年的临床应用历史,广泛地用于心血管疾病、肾炎、骨质疏松和癌症等的治疗。植物化学研究显示,香豆素类、杂萜类和黄酮类是其主要化学成分。对补骨脂化学成分研究进行系统梳理,以期为临床更安全、有效地使用补骨脂和研究补骨脂现代中药提供理论依据。

关键词：补骨脂化学成分香豆素类杂萜类黄酮类