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Strawberry Notch 1 (SBN01) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice

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Asian Journal of Andrology 2019年第4期21卷 345-350页

作者： Cong shen Jun Yu Xi zhang Chen-Chen liu Yue-Shuai Guo Jia-wei Zhu ke zhang Yi Yu Ting-Ting Gao shen-Min yang Hong Li Bo Zheng xiao-yan Huang Center for Reproduction and Genetics Suzhou Municipal Hospital The Affiliated Suzhou Hospital of Nanjing Medical University Suzhou 215002 China Department of Histology and Embryology State Key Laboratory of Reproductive Medicine Nanjing Medical University Nanjing 211166 China Department of Obstetrics and Gynecology Affiliated Hospital of Jiangsu University Jiangsu University Zhenjiang 212013 China The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University Wuxi 214002 China Center of Clinical Reproductive Medicine The Affiliated Changzhou Maternity and Child Health Care Hospital of Nanjing Medical University Changzhou 213000 China

While it is known that spermatogonial stem cells (SSCs) initiate the production of male germ cells, the mechanisms of SSC self-renewal, proliferation, and differentiation remain poorly understood. We have previously identified Strawberry Notch 1 (SBN01), a vertebrate strawberry notch family protein, in the proteome profile for mouse SSC maturation and differentiation, revealing SBN01 is associated with neonatal testicular development. To explore further the location and function of SBN01 in the testes, we performed Sbnol gene knockdown in mice to study the effects of SBN01 on neonatal testicular and SSC development. Our results revealed that SBN01 is required for neonatal testicular and SSC development in mice. Particularly, in vitro Sbnol gene knockdown with morpholino oligonucleotides caused a reduction of SSCs and inactivation of the noncanonical Wnt pathway, through Jun N-terminal kinases. Our study suggests SBN01 maintains SSCs by promoting the noncanonical Wnt pathway.

关键词： noncanonical Wnt pathway spermatogonial stem cells Strawberry Notch 1 (SBN01)

Expert panel’s guideline on cervicogenic headache:The Chinese Association for the Study of Pain recommendation

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World Journal of Clinical Cases 2021年第9期9卷 2027-2036页

作者： Hong xiao Bao-Gan Peng ke Ma dong Huang Xian-Guo liu yan Lv Qing liu Li-Juan Lu Jin-Feng liu Yi-Mei Li Tao Song wei Tao Wen shen xiao-Qiu yang Lin Wang xiao-Mei zhang Zhi-Gang Zhuang Hui liu yan-Qing liu Department of Algology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Orthopedics The Third Medical CenterGeneral Hospital of the Chinese People’s Liberation ArmyBeijing 100039China Department of Algology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Algology The Third Xiangya Hospital of Central South UniversityChangsha 410013Hunan ProvinceChina Department of Physiology and Pain Research Center Sun Yat-Sen UniversityGuangzhou 510080Guangdong ProvinceChina Department of Algology Xijing HospitalAir Force Medical UniversityXi'an 710032Shaanxi ProvinceChina Department of Algology The Affiliated T.C.M Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Algology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008Jiangsu ProvinceChina Department of Algology The Second Affiliated Hospital of Harbin Medical UniversityHarbin 150001Heilongjiang ProvinceChina Department of Algology The First Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830011XinjiangChina Department of Algology The First Affiliated Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Department of Neurosurgery Shenzhen University General HospitalShenzhen 518055Guangdong ProvinceChina Department of Algology The Affiliated Hospital of Xuzhou Medical UniversityXuzhou 221002Jiangsu ProvinceChina Department of Algology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Algology The Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004Guizhou ProvinceChina Department of Algology The First Affiliated Hospital of Kunming Medical UniversityKunming 650032Yunnan ProvinceChina Department of Algology The Second Affiliated Hospital of Zhengzhou UniversityZhengzhou 450014Henan ProvinceChina Department of Algology Beijing Tiantan HospitalCapit

Cervicogenic headache(CEH)has been recognized as a unique category of headache that can be difficult to diagnose and *** China,CEH patients are managed by many different specialties,and the treatment plans remain ***,there is a great need for comprehensive evidence-based Chinese experts’recommendations for the management of *** Chinese Association for the Study of Pain asked an expert panel to develop recommendations for a series of questions that are essential for daily clinical management of patients with CEH.A group of multidisciplinary Chinese Association for the Study of Pain experts identified the clinically relevant topics in CEH.A systematic review of the literature was performed,and evidence supporting the benefits and harms for the management of CEH was ***-four recommendations were finally developed through expert consensus voting for evidence quality and recommendation *** hope this guideline provides direction for clinicians and patients making treatment decisions for the management of CEH.

关键词： Cervicogenic headache Expert recommendation Expert panel’s guideline Chinese Association for the Study of Pain Chronic pain

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卷叶木薯及其突变体叶片的比较转录组分析

作物学报 2024年第8期50卷 2143-2156页

作者：肖明昆严炜宋记明张林辉刘倩段春芳李月仙姜太玲沈绍斌周迎春沈正松熊贤坤罗鑫白丽娜刘光华云南省农业科学院热带亚热带经济作物研究所云南保山678000 热带作物生物育种全国重点实验室云南昆明650205 云南省农业科学院国际农业研究所云南昆明650205

为解析卷叶木薯叶片异常发育的分子调控机制及代谢通路,以正常卷叶木薯叶片(JY)、突变株展叶叶片(ZY)和突变株卷叶叶片(BJ)为试验材料,基于转录组测序(RNA-seq)数据进行生物信息学分析。DESeq差异分析结果显示,ZY vs BJ、JY vs ZY、JY v... 详细信息

为解析卷叶木薯叶片异常发育的分子调控机制及代谢通路,以正常卷叶木薯叶片(JY)、突变株展叶叶片(ZY)和突变株卷叶叶片(BJ)为试验材料,基于转录组测序(RNA-seq)数据进行生物信息学分析。DESeq差异分析结果显示,ZY vs BJ、JY vs ZY、JY vs BJ分别有327(255个上调,72个下调)、1085(337个上调,748个下调)、689(381个上调,308个下调)个DEGs,有19个DEGs是3个比较组共同表达的。GO功能分析显示,DEGs在刺激反应、膜的组成部分、跨膜转运蛋白活性等途径差异显著。keGG富集分析显示,DEGs在苯丙素的生物合成、植物激素信号转导、内质网中的蛋白加工等通路较为活跃。进一步对变异展叶与同株的卷叶和其他植株正常卷叶的DEGs分析发现集中富集到苯丙素的生物合成途径、淀粉和蔗糖代谢、植物激素信号转导,这可能是展叶突变体形成的关键因子。展叶与卷叶差异基因的主要keGG代谢通路有9条,重要差异基因有9个。对不同类型叶片显微分析发现突变后的展叶木薯上表皮细胞层数增加、海绵组织结构变得疏松、维管束细胞减少。研究结果为进一步理解木薯叶片异常发育的分子机制提供了理论指导,为木薯遗传改良、种质创新挖掘提供基因资源和改良策略。

关键词：木薯转录组分析突变差异基因表达

桔梗特异DNA条形码筛选、种质资源鉴定及遗传多样性分析

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药学学报 2024年第1期59卷 243-252页

作者：王馨石玥满金辉黄钰莹张晓芹安克露何高洁刘子齐关范圆郑语嫣王晓晖魏胜利北京中医药大学中药学院北京102488 北京中医药大学北京中医药研究院中药现代研究中心北京102488 北京中医药大学生命科学院北京102488 黑龙江诺初中药材种植有限公司黑龙江哈尔滨150600

中药材桔梗为桔梗科植物桔梗的干燥根,具有多种药理作用,是常用的大宗中药材。本研究利用Illumina HiSeq X Ten平台对6份来自不同产地的桔梗进行叶绿体基因组测序,筛选特异性DNA条形码,基于特异性DNA条形码对不同产区的桔梗样品的种质... 详细信息

中药材桔梗为桔梗科植物桔梗的干燥根,具有多种药理作用,是常用的大宗中药材。本研究利用Illumina HiSeq X Ten平台对6份来自不同产地的桔梗进行叶绿体基因组测序,筛选特异性DNA条形码,基于特异性DNA条形码对不同产区的桔梗样品的种质资源和遗传多样性进行分析。6份桔梗叶绿体基因组全长为172260~172275 bp,均呈现典型的环状四分体结构,编码141个基因。根据比较基因组学分析和扩增效率分析发现trnG-UCC和ndhG_ndhF可作为潜在的桔梗种内种质资源鉴定的特异性DNA条形码。对来自9省15个产地305份桔梗样品的trnG-UCC和ndhG_ndhF进行PCR扩增和序列分析,结果表明trnG-UCC和ndhG_ndhF分别有5和11个变异位点,分别鉴定到5和7个单倍型;两段序列联合分析鉴定13个单倍型(Hap1~Hap13),其中占比最多的是Hap4,其次是Hap1。3个产地拥有的特异单倍型,可作为该产地的DNA分子标签与其他产地的桔梗种质资源进行区分。单倍型多样性、核苷酸多样性和遗传距离分别为0.94、4.79×10^(-3)和0.0000~0.0203,表明桔梗在物种水平上有较高的遗传多样性,种内各单倍型之间亲缘关系比较接近。本研究为桔梗产地鉴定、种质资源保护利用和分子育种等工作奠定基础。

关键词：桔梗叶绿体基因组 DNA条形码种质资源遗传多样性

基于生化指标与代谢组学特征关联探索热毒宁注射液与青霉素注射液序贯用药的风险“时间窗”

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药学学报 2024年第7期59卷 2098-2107页

作者：张明亮陈毓龙王晓艳陈小菲张辉吴娅丽杨柳青张书琦牛璐冯科冉李伟霞唐进法河南中医药大学第一附属医院药学部河南省中药临床应用、评价与转化工程研究中心河南省中药临床药学中医药重点实验室河南省中药安全评价与风险防控工程研究中心河南郑州450003 河南中医药大学河南郑州450046 河南中医药大学呼吸疾病中药防治省部共建协同创新中心河南郑州450046

采用检测血清生化指标与血浆代谢组学特征关联探索热毒宁注射液(Reduning,RDN)与青霉素注射液(penicillin G,PG)序贯用药风险“时间窗”,以期降低二者联用引发不良反应发生风险。所有动物实验操作和福利均遵循河南中医药大学第一附属实... 详细信息

采用检测血清生化指标与血浆代谢组学特征关联探索热毒宁注射液(Reduning,RDN)与青霉素注射液(penicillin G,PG)序贯用药风险“时间窗”,以期降低二者联用引发不良反应发生风险。所有动物实验操作和福利均遵循河南中医药大学第一附属实验动物伦理与动物福利委员会要求(批号:YFYDW2020002)。利用ELISA检测大鼠血清中生化指标的含量变化检测相关过敏指标,判定出RDN联用PG能引起由补体途径激活后的类过敏反应(pseudo-allergic reactions,PARs)。进一步对二者设置不同时间给药间隔(1.5、2、3.5、4、6和8 h PG+RDN)进行考察,发现在3.5 h以内序贯给药,均能引起明显的PARs;而给药间隔超过4 h后PARs则明显减轻。同时采用LC-MS进行血浆代谢组学分析,平行比较大鼠的血清生化指标水平与血浆代谢轮廓特征,筛选出22个差异代谢物在3.5 h前后呈现出与生化指标相似或相反的变化趋势。并富集到花生四烯酸代谢、甾体激素生物合成代谢、亚油酸代谢、甘油磷脂代谢、色氨酸代谢等10条类过敏相关通路。综上所述,RDN和PG序贯使用所导致的药物不良反应确实存在风险“时间窗”现象,在“时间窗”之后的间隔用药能有效降低不良反应发生风险。

关键词：中西药联用药物不良反应时间窗热毒宁注射液青霉素注射液类过敏反应代谢组学花生四烯酸代谢

Preparation and property of CL-20/BAMO-THF energetic nanocomposites

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Defence Technology（防务技术） 2019年第3期15卷 306-312页

作者： Teng Chen yan zhang Shuang-feng Guo liu-ming Zhao wei Chen Ga-zi Hao Lei xiao Xiang ke wei Jiang National Special Superfine Powder Engineering Research Center of China School of Chemical Engineering Nanjing University of Science and Technology Nanjing 210094 PR China Xi'an Modern Chemistry Research Institute Shaanxi 710065 PR China

A sol-gel freezing-drying method was utilized to prepare energetic nanocomposites based on 2, 4, 6, 8,10, 12-hexanitro-2, 4, 6, 8, 10, 12-hexaazaisowurtzitane(CL-20) with 3, 3-Bis(azidomethyl) oxetanetetrahydrofuran copolymer(BAMO-THF) as energetic gel matrix. Scanning electron microscopy(SEM),X-ray diffraction(XRD), Raman, Fourier-transform infrared spectroscopy(FT-IR) and differential thermal analyser(DTA) were utilized to characterize the structure and property of the resultant energetic nanocomposites. Compared with raw CL-20, the average particle sizes of CL-20 in CL-20/BAMO-THF energetic nanocomposites were decreased to nano scale and the morphologies of CL-20 were also changed from prismatic to spherical. FT-IR detection revealed that CL-20 particles were recrystallized in BAMO-THF gel matrix during the freezing-drying process. The thermal decomposition behaviors of the energetic nanocomposites were investigated as well. The thermolysis process of CL-20/BAMO-THF nanocomposites was enhanced and the activation energy was lower compared with that of raw CL-20,indicating that CL-20/BAMO-THF nanocomposites showed high thermolysis activity. The impact sensitivity tests indicated that CL-20/BAMO-THF energetic nanocomposites presented low sensitivity performance.

关键词： CL-20/BAMO-THF nanocomposites Kinetic Thermodynamic Impact sensitivity

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小胶质细胞在自杀中的作用及其机制

中国临床心理学杂志 2024年第2期32卷 273-276,272页

作者：杨艳杰乔可欣卜天一阚越粹杨佳润邱晓惠乔正学周佳玮胡晓朦刘璇张计丽哈尔滨医科大学心理科学与健康管理中心哈尔滨150081 黑龙江大学教育科学研究院哈尔滨150080

自杀的发生率日益提高,但是其发生机制尚不清楚。近年来的研究表明神经生物学因素与自杀有着密切的关系。作为中枢神经系统的重要组成部分,小胶质细胞对神经递质代谢、免疫调节和神经炎症等都有较大影响,过度激活的小胶质细胞被认为在... 详细信息

自杀的发生率日益提高,但是其发生机制尚不清楚。近年来的研究表明神经生物学因素与自杀有着密切的关系。作为中枢神经系统的重要组成部分,小胶质细胞对神经递质代谢、免疫调节和神经炎症等都有较大影响,过度激活的小胶质细胞被认为在自杀中发挥了重要作用。本文将对小胶质细胞状态与自杀的关系,以及小胶质细胞介导的神经炎症、神经免疫、应激反应过程影响自杀行为的相关机制进行综述,以期为自杀的神经机制研究提供思路,为自杀预防策略的制定提供理论依据。

关键词：小胶质细胞自杀神经炎症神经免疫

Evaluation of a new frequency-volume chart for children with primary monosymptomatic nocturnal enuresis:a prospective,comparative study

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World Journal of Pediatrics 2021年第6期17卷 643-652页

作者： Jing-Jing Wang dong-Jie Wang Hai-dong Fu De-Xuan Wang Qing yang xiao-Jie Peng Rui Fu Huan-Dan yang Rui-Feng zhang xiao-Wen Wang Jiang-wei Luan Fei Zhao Qiu-Xia Chen Fang-Fang Liang Lu Cao Jian-Jiang zhang ying-Jie Li yang dong Hui-Mei Huang ying Bao xiao-Yu shen wei Zhou Guang-Hai Cao Cui-Hua liu yan-yan Jin Mei-Lin Ma Li Jiang Qian shen Hong Xu Jian-Hua Mao Department of Nephrology TChildren's Hospital of Zhejiang University School of MedicineNational Clinical Research Center of Child HealthHangzhouChina Department of Nephrology The Second Attiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhouChina Department of Nephrology Jiangxi Provincial Children's HospitalNanchangChina Department of Nephrology Xuzhou Children's HospitalXuzhou Medical UniversityXuzhouChina Department of Nephrology Wuhan Children's Hospital(Wuhan Maternal and Child Healthcare Hospital)Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Nephrology Children's Hospital of Nanjing Medical UniversityNanjingChina Department of Rheumatology Shenzhen Children's HospitalShenzhenChina Department of Pediatrics The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenanChina Department of Nephrology Guangzhou Women and Children's Medical CenterGuangzhouChina Department of Nephrology Anhui Provincial Children's HospitalHefeiChina Department of Nephrology The Affiliated Children Hospital of Xi'an Jiaotong UniversityXi'anChina Department of Nephrology Shanghai Children's Medical CenterShanghaiChina Department of Nephrology and Rheumatology Children's Hospital Affiliated to ZhengZhou UniversityHenan Children's HospitalZhengZhou Children's HospitalZhengZhouChina Medical Affairs Department Ferring Pharmaceuticals(Asia)Company LimitedShanghaiChina Marketing Department Ferring Pharmaceuticals(Asia)Company LimitedShanghaiChina Department of Nephrology The Children's Hospital of Fudan UniversityShanghaiChina

Introduction To improve compliance with voiding diaries in children with primary monosymptomatic nocturnal enuresis(PMNE),a new modified 3-day weekend frequency-volume chart(FVC)was designed,and the compliance and validity of this modified FVC was evaluated by comparing with the International Children's Continence Society(ICCS)recommended voiding *** A total of 1200 patients with PMNE were enrolled in the study from 13 centers in China and were randomly assigned to record this modified FVC or the ICCS-recommended voiding *** primary outcome measure was the compliance,assessed by comparing the completing index and the quality score of diaries between two *** secondary outcome measure was the validity,evaluated by comparing the constituent of subtypes,micturition parameters and response rate to *** Among the 1200 participants enrolled in the study,447 patients completed the ICCS-recommended voiding diary and 469 completed the modified *** diurnal completing index and the quality score of the modified FVC group were better than those of the ICCS *** addition,there was no significant difference between these two groups in the subtype classification,or in the response rate to *** The modified FVC could be applied to obtain the voiding characteristics of children with PMNE as the ICCS-recommended voiding diary does and offers a reasonable and better choice for children with PMNE from the unselected population in the future.

关键词： Children Compliance Nocturnal enuresis Voiding diary

Mechanism of progressive failure of a slope with a steep joint under the action of freezing and thawing:model test

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Journal of Mountain Science 2022年第10期19卷 2999-3012页

作者： LI Cong zhang Rong-tang ZHU Jie-bing LU Bo shen xiao-ke WANG xiao-wei liu Jie-sheng WU Liang-liang zhang Xin-zhou School of Civil Engineering and Architecture Wuhan Polytechnic UniversityWuhan 430023China key Laboratory of Geotechnical Mechanics and Engineering of Ministry of Water Resources Changjiang River Scientific Research InstituteWuhan 430010China key Laboratory of Ministry of Education for Geomechanics and Embankment Engineering Hohai UniversityNanjing 210098China

The stability of slope rock masses is influenced by freeze-thaw cycles in cold region,and the mechanism of stability deterioration is not *** order to understand the damage and progressive failure characteristics of rock masses under the action of freezing and thawing,a model test was conducted on slope with steep joint in this *** temperature,frost heaving pressure and deformation of slope rock mass were monitored in real-time during the test and the progressive failure mode was *** experimental results show that the temperature variations of cracking and the rock mass of a slope are *** are obvious latent heat stages in the temperature-change plot in the crack,but not in the slope rock *** frost heaving effect in the fracture is closely related to the constraint conditions,which change with the deformation of the *** frost heaving pressure fluctuates periodically during freezing and continues to decrease during *** surface deformation of the rock mass increases during freezing,and the deformation is restored when it ***-thaw cycling results in residual deformation of the rock mass which cannot be fully *** shows that the rock mass at the free side of the steep-dip joint rotates slightly under the frost heaving effect,causing fracture *** fracture propagation pattern is a circular arc at the beginning,then extends to the possible sliding direction of the rock *** heaving force and fracture water pressure are the key factors for the failure of the slope,which can cause the crack to penetrate the rock mass,and a landslide ensues when the overall anti-sliding resistance of the rock mass is overcome.

关键词： Rock slope Fractured rock mass Freeze-thaw cycle Model experiment Stability degradation mechanism Failure mode