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Environmental Science and Ecotechnology 2022年第2期10卷 38-44页

作者： Chuan Yu Jie He Shenyu lan Wanqian Guo Mingshan Zhu Guangdong Key Laboratory of Environmental Pollution and Health School of EnvironmentJinan UniversityGuangzhou511443PR China State Key Laboratory of Urban Water Resource and Environment Harbin Institute of TechnologyHarbin150090PR China

The efficient activation and utilization of peroxymonosulfate(PMS)in PMS-based advanced oxidation processes is a high-priority target for the removal of organic *** work introduces a water vortex-driven piezoelectric effect from few-odd-layered MoS_(2)into the PMS activation to remove benzotriazole(BTR)and other organic contaminants from the *** 91.1%of BTR can be removed by the MoS_(2)piezo-activated PMS process with a reaction rate constant of 0.428 min1,which is 2.09 times faster than the sum of the individual MoS_(2),water vortex,and piezocatalysis ***,the PMS utilization efficiency reached 0.0147 in the water vortex-driven piezo-activation system,which is 3.97 times that of the sum from the vortex/PMS and MoS_(2)/PMS *** results demonstrate that the presence of MoS_(2)under a water vortex can trigger a piezoelectric potential and generate abundant free electrons to activate PMS to generate various active species for degradation of organic contaminants.

关键词： Water vortex-driven Peroxymonosulfate utilization Piezoelectric activation Organic contaminants Few-odd-layered MoS2

Metastasis to the thyroid gland from primary breast cancer presenting as diffuse goiter:A case report and review of literature

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World Journal of Clinical Cases 2022年第3期10卷 1106-1115页

作者： Wen Wen Heng Jiang Hsin-Yu Wen Yu-lan Peng Department of Ultrasound West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina College of Medicine University of Nebraska Medical CenterOmahaNE 68133United States Department of Pathology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina

BACKGROUND Metastasis to the thyroid gland(TM)from primary breast cancer is uncommon and usually presents as thyroid nodules;however,diffuse goiter without thyroid nodules is the first sign of TM in rare *** metastases(SMs)to the lymph nodes in breast cancer,defined as discontiguous higher-level metastases in the absence of lower levels of contiguous metastases,have been reported in the contralateral cervical area of the primary tumor site in rare *** SUMMARY A 49-year-old previously healthy Chinese woman was diagnosed with right lateral invasive ductal carcinoma and underwent neoadjuvant chemotherapy treatment and bilateral mastectomy with axillary lymph node *** malignancy of the left breast or axillary or distant metastases were identified ***,enlarged left cervical lymph nodes were detected 36 mo after surgery,and rapidly enlarging thyroid glands without nodules were detected 42 mo after ***-needle aspiration cytology was performed on the left cervical lymph nodes and left lobe of the thyroid,which were both revealed to contain metastases from the primary breast ***,the immunostaining profiles changed in the process of *** patient was discharged with the NP(vinorelbine and cisplatin)regimen for subsequent treatment,and stable disease was determined when the curative effect was *** Diffuse goiter may be the first sign of TM,and enlarged lymph nodes in the contralateral cervical area may be SMs of primary breast cancer.

关键词： Metastases to the thyroid gland Diffuse goiter Cervical lymph node recurrence Breast cancer Case report

Evaluation preparation technology of Xiaochaihu granules using fingerprint-peak pattern matching

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Journal of Pharmaceutical Analysis 2011年第2期1卷 119-124页

作者： Yu-Qiong Wu Yu- Qiang Gou Jing Han ying-Yan Bi Shi-lan Feng Fang-Di Hu Chun- Ming Wang School of Pharmacy Lanzhou University Lanzhou 730000 China the Second People's Hospital of Gansu Province Lanzhou 730000 China Center for Disease Control and Prevention Lanzhou Military Area Command of Chinese People's Liberation Army Lanzhou 730000 China College of Chemistry and Chemical Engineering Lanzhou University Lanzhou 730000 China

An approach was proposed to evaluate preparation technology by means of fingerprint-peak matching technology of high performance liquid chromatography with diode array detector （HPLC-DAD）. Similarity and hierarchical clustering analysis （HCA） were applied to identify the 15 batches of Xiaochaihu granules from different manufacturers and our laboratory, and peak pattern matching between the composite formulae and Radix Bupleuri Chinensis, which was one of the main ingredients of Xiaochaihu granules, was utilized to evaluate the preparation technology of Xiaochaihu granules via the indexes of the relative deviation of retention time （RT） and UV spectrum feature similarity of their corresponding peaks. Eleven matching peaks were found between Xiaochaihu granules and Radix Bupleuri Chinensis. However, the saikosaponin A and saikosaponin D, which are the important active components in Radix Bupleuri Chinensis, were not found in Xiaochaihu granules from any manufacturers. The peak areas of 11 characteristic peaks of Xiaochaihu granules samples formed a matrix of 11 × 15. The result of HCA showed that Xiaochaihu granules samples were divided into four kinds of category. Xiaochaihu granules samples from the same manufacturer were basically clustered of the same category. The results suggested that the saikosaponin A and saikosaponin D are prone to structural transformation under the condition of decoction and in the presence of the organic acidic components. These active components, existing in raw herb, might transform to a series of non-active secondary saikosaponin due to unfavourable preparation technology. So the conventional decoction-based preparation technology of Xiaochaihu granules might greatly affect its quality and therapeutic effectiveness. This study demonstrates that fingerprint-peak matching technology can not only be used for quality control of this composite formulae, but also provide some guidance for preparation technology of Xiaochaihu granules.

关键词： Xiaochaihu granules Radix Bupleuri Chinensis fingerprint-peak matching hierarchical clustering analysis

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银杏叶提取物对脑卒中后抑郁大鼠的影响

中国临床药理学杂志 2024年第13期40卷 1933-1937页

作者：谢思敏王有琼莫苗苗王冬怡陈海兰杨斌广西医科大学药学院广西南宁530022 广西医科大学第一临床医学院广西南宁530022

目的观察银杏叶提取物(GBE)对脑卒中后抑郁(PSD)模型大鼠抑郁样行为的干预作用,并研究其通过调控Toll样受体4/核因子-κB(TLR4/NF-κB)通路抑制神经炎症的作用机制。方法大鼠随机分为假手术组、脑缺血组、PSD组、帕罗西汀组、银杏叶提... 详细信息

目的观察银杏叶提取物(GBE)对脑卒中后抑郁(PSD)模型大鼠抑郁样行为的干预作用,并研究其通过调控Toll样受体4/核因子-κB(TLR4/NF-κB)通路抑制神经炎症的作用机制。方法大鼠随机分为假手术组、脑缺血组、PSD组、帕罗西汀组、银杏叶提取物低剂量(GBE-L)组及银杏叶提取物高剂量(GBE-H)组,每组10只。除假手术组外,其余各组大鼠均进行大脑中动脉栓塞术(MCAO)制备左侧脑缺血再灌注模型。除假手术组和脑缺血组外,其余各组大鼠予慢性不可预知温和刺激(CUMS)建立PSD大鼠模型,持续刺激8周。刺激4周后,帕罗西汀组、GBE-L及GBE-H组分别给予帕罗西汀5 mg·kg^(-1)、GBE50 mg·kg^(-1)、GBE100 mg·kg^(-1)治疗,假手术组、脑缺血及PSD组给予等体积0.9%NaCl,连续灌胃给药28 d。在造模第4周和第8周,分别测定大鼠体质量和糖水偏好率。用酶联免疫吸附测定(ELISA)法检测血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平及大脑皮质中去甲肾上腺素(NE)、5-羟色胺(5-HT)、多巴胺(DA)水平;用聚合酶链反应法检测大鼠海马Tlr4、Nfkb1、核因子κB激酶亚基β抑制因子(Ikbkb)mRNA水平;用蛋白质印迹法检测大鼠海马组织NF-κB、核因子κB抑制蛋白α(IKBα)以及磷酸化核因子κB抑制蛋白α(p-IKBα)蛋白表达水平。结果假手术组、脑缺血组、PSD组、帕罗西汀组、GBE-L及GBE-H组给药治疗后大鼠体质量分别为(427.10±6.36)、(403.10±7.37)、(310.10±9.71)、(355.00±4.03)、(347.90±9.88)和(391.90±5.07)g;给药治疗后大鼠糖水消耗率分别为(93.93±1.78)%、(91.57±1.03)%、(54.72±7.34)%、(88.35±4.36)%、(63.55±12.73)%和(81.04±4.31)%;大脑皮质NE含量分别为(1951.14±52.86)、(1827.27±23.63)、(1662.12±35.92)、(2033.58±72.28)、(1887.31±33.07)和(2175.00±42.54)pg·mL^(-1);大脑皮质5-HT含量分别为(237.07±8.86)、(226.15±10.27)、(214.51±3.46)、(297.13±5.79)、(274.14±7.63)和(285.34±8.72)ng·mL^(-1);大脑皮质DA含量分别为(1531.11±47.26)、(1209.89±58.09)、(1143.15±36.31)、(1812.67±51.28)、(1651.56±31.82)和(1853.33±20.42)pg·mL^(-1)。与PSD组相比,GBE能显著增加大鼠体质量(P<0.01),提高大鼠糖水消耗率,表现出抗抑郁样行为作用。GBE显著降低血清中TNF-α、IL-1β水平(均P<0.01),增加大脑皮质中NE、5-HT、DA水平(均P<0.01);下调Tlr4、Nfkb1、Ikbkb mRNA水平(P<0.05,P<0.01);减少NF-κB蛋白表达(P<0.01),降低IKBα磷酸化水平(P<0.01)。结论银杏叶提取物可减轻PSD大鼠抑郁样行为,具有抗抑郁作用,其机制与抑制TLR4/NF-κB通路减轻神经炎症有关。

关键词：银杏叶提取物脑卒中抑郁神经炎症 Toll样受体4(TLR4) 核因子-κB(NF-κB)

Biallelic mutations in WDR12 are associated with male infertility with tapered-head sperm

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Asian Journal of Andrology 2023年第3期25卷 398-403页

作者： Juan Hua lan Guo Yao Yao Wen Hu Yang-Yang Wan Bo Xu Department of Biochemistry and Molecular Biology School of Basic Medical SciencesAnhui Medical UniversityHefei 230032China Metabolic Disease Research Center School of Basic MedicineAnhui Medical UniversityHefei 230032China Hefei Xingzhike Biotechnology Co. Ltd.Hefei 230000China Reproductive and Genetic Hospital The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China

Teratozoospermia is a rare disease associated with male *** recurrent genetic mutations have been reported to be associated with abnormal sperm morphology,but the genetic basis of tapered-head sperm is not well *** this study,whole-exome sequencing(WES)identified a homozygous WD repeat domain 12(WDR12;***162Ala/c.484T>G)variant in an infertile patient with tapered-head spermatozoa from a consanguineous Chinese *** analysis predicted this mutation to be a pathogenic *** verify the effect of this variant,we analyzed WDR12 protein expression in spermatozoa of the patient and a control individual,as well as in the 293T cell line,by Western blot analysis,and found that WDR12 expression was significantly *** understand the role of normal WDR12,we evaluated its mRNA and protein expression in mice at different *** observed that WDR12 expression was increased in pachytene spermatocytes,with intense staining visible in round spermatid *** on these results,the data suggest that the rare biallelic pathogenic missense variant(***162Ala/c.484T>G)in the WDR12 gene is associated with tapered-head *** addition,after intracytoplasmic sperm injection(ICSI),a successful pregnancy was *** finding indicates that infertility associated with this WDR12 homozygous mutation can be overcome by *** present results may provide novel insights into understanding the molecular mechanisms of male infertility.

关键词： male infertility tapered-head sperm teratozoospermia WD repeat domain 12(WDR12)

Systematic identification and functional analysis of long noncoding RNAs involved in indoxacarb resistance in Spodoptera litura

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Insect Science 2022年第6期29卷 1721-1736页

作者： Li Shi Wen-Lin Li Hai-Xin Zeng Yao Shi Xiao-lan Liao Hunan Provincial Engineering and Technology Research Center for Bio-pesticide and Formulation Processing College of Plant ProtectionHunan Agricultural UniversityChangshaChina Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases andInsectPests ChangshaChina

Long noncoding RNAs(lncRNAs)are noncoding transcripts that are more than 200 nucleotides *** play essential roles in regulating a variety of biological processes in many species,including insects,and some lncRNAs have been found to be associated with insecticide ***,the characteristics and biological func-tions of lncRNAs involved in indoxacarb resistance are unknown in Spodoptera *** performed RNA sequencing in the SS,InRS,and FInRS of *** and identified 11978 lncRNAs,including 3136 intergenic lncRNAs,7393 intronic lncRNAs,and 1449 anti-sense *** with the SS,51 lncRNAs were upregulated and 134 lncRNAs were downregulated in the two resistant strains,and 908 differentially expressed mRNAs were predicted as the target genes of the 185 differentially expressed *** analysis showed that 112 of differentially expressed lncRNAs may be associated with in-doxacarb resistance by regulating the expression of 14 P450s,seven CCEs,one GST,six UGTs,five ABC transporters,and 24 cuticle protein genes,and 79 of differentially ex-pressed lncRNAs may regulate the expression of 14 detoxification genes and 19 cuticle protein genes to participate in indoxacarb resistance by sponging 10 ***-ingly,47 of differentially expressed lncRNAs may mediate indoxacarb resistance through both lncRNA-mRNA and lncRNA-miRNA-mRNA regulatory ***,quantitative PCR,RNA interference,and indoxacarb bioassay analyses indicated that over-expressed LNC_004867 and LNC_006576 were involved in indoxacarb *** study provides comprehensive information for lncRNAs of ***,and presents evidence that lncRNAs havekey roles in conferring insecticide resistance in ***.

关键词： indoxacarb resistance long noncoding RNAs regulatory pathways RNA sequencing Spodopteralitura

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新型冠状病毒中和抗体生物学活性的测定

中国药学杂志 2024年第4期59卷 353-362页

作者：俞小娟王安陈奔蒋雯李纲王荣娟张姣王文波段茂芹于传飞王兰中国食品药品检定研究院国家卫生健康委员会生物技术产品检定方法及其标准化重点实验室国家药品监督管理局生物制品质量研究与评价重点实验室北京102629 迈威(上海)生物科技股份有限公司上海201210

目的开发针对新型冠状病毒中和抗体生物学活性及免疫学特性分析的方法。方法使用生物膜层干涉法(biolayer interferometry,BLI)分析9MW3311中和抗体Fab和S1-RBD的亲和力常数;分别使用酶联免疫吸附法(enzyme linked immunosorbent assay,... 详细信息

目的开发针对新型冠状病毒中和抗体生物学活性及免疫学特性分析的方法。方法使用生物膜层干涉法(biolayer interferometry,BLI)分析9MW3311中和抗体Fab和S1-RBD的亲和力常数;分别使用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)和流式细胞分选法评价中和抗体结合片段(antibody binding fragment,Fab端)和S蛋白的相对结合活性和对ACE2的阻断结合活性;使用假病毒体系评价中和抗体的体外细胞学活性;使用表面等离子共振法(surface plasmon resonance,SPR)测定抗体Fc段与Fcγ和FcRn受体的亲和力常数;使用ELISA法测定抗体Fc段和C1q受体的结合活性;采用PBMC法确定中和抗体的体依赖细胞介导的细胞毒性作用(antibody-dependent cell-mediated cytotoxicity,ADCC)、补体(First subcomponent of the C1 complex)介导的细胞毒性作用(complement dependent cytotoxicity,CDC);使用假病毒系统评价中和抗体的依赖性感染增强作用(antibody-dependent enhancement,ADE)效应。结果3批9MW3311中和抗体和参比品与S1-RBD的亲和力常数KD(mol·L^(-1))值分别为1.15×10^(-9),1.01×10^(-9),1.15×10^(-9)和9.45×10^(-10);ELISA和FACS分别测得3批中和抗体相对参比品的结合活性为101%、96%、100%及98%、113%、108%;ELISA和FACS分别测得3批中和抗体相对参比品对ACE2的阻断活性为100%、95%、91%及94%、101%、94%;3批中和抗体相对参比品对假病毒的中和活性分别91%、93%、108%;3批9MW3311中和抗体和参比品分别与FcγR和FcRn均有同等水平的亲和力;9MW3311无ADCC和CDC活性;LALA突变的中和抗体可有效避免ADE效应。结论初步建立了新型冠状病毒中和抗体生物学活性及免疫学特性分析的方法,可用于该类型制品的常规质量控制和放行。

关键词：新型冠状病毒中和抗体生物学活性免疫学特性

Biological functions and potential implications of circular RNAs

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The Journal of Biomedical Research 2023年第2期37卷 89-99页

作者： lan Ma Haiyan Chu Meilin Wang Zhengdong Zhang Department of Genetic Toxicology The Key Laboratory of Modern Toxicology of Ministry of EducationCenter for Global HealthSchool of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Department of Environmental Genomics Jiangsu Key Laboratory of Cancer BiomarkersPrevention and TreatmentCollaborative Innovation Center for Cancer Personalized MedicineNanjing Medical UniversityNanjingJiangsu 211166China.

Circular RNAs(circRNAs) are characterized by a covalent closed-loop structure with an absence of both 5′ cap structure and 3′ polyadenylated tail. Numerous studies have found that circRNAs play an important role in various diseases and have a variety of biological regulatory mechanisms, including acting as microRNA sponges,interacting with proteins, modulating the expression of related genes and translating into peptides or *** have also been used as biomarkers for a number of diseases, which could improve clinical *** review summarizes the most recent advances in biogenesis and knowledge of the biological functions of circRNAs as well as the related bioinformatics databases. We specifically describe developments in understanding of circRNA functions in the field of environmental exposure-induced diseases. Finally, we focus on potential clinical implications of circRNAs to facilitate their clinical transformation into disease treatment.

关键词： circular RNAs biogenesis mechanisms database biomarkers

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科研管理信息化对医院科研工作的影响及对策

Relationship Between Lodging Resistance and Chemical Contents in Culms and Sheaths of Japonica Rice During Grain Filling

科学与信息化 2024年第6期 150-152页

作者：高韦吴岚巩欣媛刘蕾天津市第一中心医院天津 300192

医院科研管理工作涉及的内容和事项比较多,需要做好从项目申请立项到科研成果转化全过程、精细化的管理.推进科研管理信息化能更好地协助工作实施,提高医院科研管理工作成效,助力医院的可持续健康发展.本文主要分析科研管理信息化对医... 详细信息

医院科研管理工作涉及的内容和事项比较多,需要做好从项目申请立项到科研成果转化全过程、精细化的管理.推进科研管理信息化能更好地协助工作实施,提高医院科研管理工作成效,助力医院的可持续健康发展.本文主要分析科研管理信息化对医院科研工作产生的影响,就如何提高科研管理信息化水平提出一些建议对策.

关键词：科研管理信息化医院科研工作影响与对策

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Rice science 2010年第4期17卷 311-318页

作者： ZHANG Feng-zhuan JIN Zheng-xun MA Guo-hui SHANG Wen-nan LIU Hai-ying Xu Mei-lan LIU Yan Agricultural College Northeast Agricultural University Harbin 150030 China China National Hybrid Rice R ＆ D Center Changsha 41 O125 China Jilin Province Ping＇an Seeds Company Changchun 133000 China

To understand the relationship between lodging resistance and chemical component contents in culms and leaf sheaths of rice, the physical strength and maximum bearing capacity of culm, and the contents and amounts of potassium （K） silicon （Si） and soluble sugars in culms and leaf sheaths were investigated using four japonica rice varieties with different lodging resistance characteristics during grain filling. There were significant differences in the total amounts of K, Si and soluble sugars in culms and leaf sheaths among the tested rice varieties. The difference in the total amount of Si was greater than that of K or soluble sugars. The physical strength and maximum bearing capacity of culm continuously decreased from heading to ripening, with a rapid decrease at the dough stage. However, the contents and total amounts of K and Si in culms and the Si content in leaf sheaths gradually increased and an accumulation of K and Si in culms was exhibited, whereas the content and total amount of K and the total amount of Si in leaf sheaths gradually decreased and an exportation of K and Si in leaf sheaths was presented. The physical strength was positively and significantly correlated with the total amounts of K and Si in culms during grain filling except that at the heading stage, the total amount of soluble sugars in culms at the heading and milky stages, the total amounts of Si and soluble sugars in leaf sheaths at the heading stage, the total amount of K in leaf sheaths at the heading and milky stages, and the maximum bearing capacity during grain filling. It is suggested that the lodging resistance of japonica rice would be improved by increasing the amount of soluble sugars in plants at the early filling stage, and enhancing the amount of Si in plants during grain filling through topdressing Si fertilizer at the early filling stage.

关键词： japonica rice grain filling lodging resistance chemical component content dynamic change