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Adolescent alcohol exposure changes RNA modifications in adult brain by mass spectrometry-based comprehensive profiling analysis

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Chinese Chemical Letters 2024年第1期35卷 461-464页

作者： ying-ying chen Zhu Gui Di Hu Meng-Yuan chen Jin-Gang He Si-Yu Yu Yu-Qi Feng Jie Wang Bi-Feng Yuan College of Chemistry and Molecular Sciences Research Center of Public HealthRenmin Hospital of Wuhan UniversityWuhan UniversityWuhan 430072China Department of Radiation and Medical Oncology Zhongnan Hospital of Wuhan UniversitySchool of Public HealthWuhan UniversityWuhan 430071China Cancer Precision Diagnosis and Treatment and Translational Medicine Hubei Engineering Research Center Zhongnan Hospital of Wuhan UniversityWuhan Research Center for Infectious Diseases and CancerChinese Academy of Medical SciencesWuhan 430071China Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsNational Center for Magnetic Resonance in WuhanWuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of Sciences-Wuhan National Laboratory for OptoelectronicsWuhan 430071China Phase I Clinical Trial Laboratory Zhongnan Hospital of Wuhan UniversityWuhan 430071China Institute of Neuroscience and Brain Diseases Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyang 441000China University of Chinese Academy of Sciences Beijing 101408China

Alcohol consumption is one of the leading causes of death worldwide.Adolescence is a critical period of structural and functional maturation of the brain.Adolescent alcohol use can alter epigenetic modifications.However,little is known on the long-term effects of alcohol consumption during adolescence on RNA epigenetic modifications in brain.Herein,we systematically explored the long-term effects of alcohol exposure during adolescence on small RNA modifications in adult rat brain tissues by comprehensive liquid chromatography-electrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS)analysis.We totally detected 26 modifications in small RNA of brain tissues.Notably,we observed most of these modifications were decreased in brain tissues.These results suggest that alcohol exposure during adolescence may impose a long-lasting impact on RNA modifications in brain tissues.This is the first report that alcohol use during adolescence can alter RNA modifications in adult brain.Collectively,this study suggests a long-term adverse effects of alcohol consumption on brain from RNA epigenetics angle by comprehensive mass spectrometry analysis.

关键词： Mass spectrometry RNA modification Adolescence Alcohol exposure Brain

Coinfection of Streptococcus suis and Nocardia asiatica in the human central nervous system:A case report

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World Journal of Clinical Cases 2022年第18期10卷 6283-6288页

作者： ying-ying chen Xin-Hong Xue Department of Neurology Liaocheng People’s HospitalLiaocheng 252000Shandong ProvinceChina

BACKGROUND Streptococcus suis(S.suis)is an anthropozoonotic pathogen that shows clinical manifestations of meningitis,septicemia,and arthritis in infected humans.Nocardia is another type of anthropozoonotic bacteria,with clinical manifestations of skin,lung,and brain abscesses in infected humans.Few intracranial infections caused by S.suis or Nocardia have been reported.To the best of our knowledge,no study has reported a patient with simultaneous intracranial infection by S.suis and Nocardia.CASE SUMMARY A 66-year-old male presented at Liaocheng People’s Hospital(Liaocheng,Shandong Province,China)reporting dizziness with nausea and vomiting.Metagenomic next-generation sequencing(m NGS)was performed on cerebrospinal fluid for examination,and the patient was diagnosed with suppurative meningitis caused by S.suis infection.He received anti-infection treatment with penicillin sodium and ceftriaxone.The patient’s condition initially improved but then deteriorated.Further m NGS of cerebrospinal fluid revealed both S.suis and Nocardia.Imaging examination revealed a brain abscess.Furthermore,a mixed infection of S.suis and Nocardia was detected in the patient’s central nervous system.The patient was treated with antibiotics and sulfamethoxazole.He was discharged after his condition improved.CONCLUSION This case shows that the disease can be recurrent in patients with intracranial infection of a rare pathogen.The possibility of mixed infection should also be considered,especially in patients treated with immunosuppressive agents.m NGS of cerebrospinal fluid is a supplement to conventional microbial pathogen identification methods.Patients with unknown pathogen diagnosis,early extensive use of antibiotics and infection with rare pathogens can be diagnosed by the combination of conventional methods and m NGS of cerebrospinal fluid.

关键词： Streptococcus suis Nocardia Meningitis Brain abscess Case report

Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases of the central nervous system after empirical treatment

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World Journal of Clinical Cases 2022年第22期10卷 7760-7771页

作者： ying-ying chen Yan Guo Xin-Hong Xue Feng Pang Department of Neurology Liaocheng People's HospitalLiaocheng 252000Shandong ProvinceChina Central Laboratory Liaocheng People's HospitalLiaocheng 252000Shandong ProvinceChina

BACKGROUND The diagnostic value of metagenomic next-generation sequencing(mNGS)in central nervous system(CNS)infectious diseases after empirical treatment has not been reported.AIM To investigate the diagnostic value of mNGS of cerebrospinal fluid(CSF)in the empirically treated CNS infectious diseases.METHODS A total of 262 CSF samples from patients with suspected CNS infections were collected between August 2020 and December 2021.Both mNGS and conventional methods were used for testing.The conventional methods included microbial culture,smear,polymerase chain reaction,etc.RESULTS Among 262 suspected cases,183 cases(69.84%)were diagnosed as CNS infection,including 86 cases of virus infection(47.00%),70 cases of bacterial infection(38.25%)and 27 cases of fungal infection(14.76%).The sensitivity and specificity of mNGS were 65.6%(95%CI:58.2%-72.3%)and 89.6%(95%CI:79.1%-95.3%),respectively.The PPV of mNGS was 94.5%(95%CI:88.6%-97.6%),and the NPV was 48.8%(95%CI:39.7%–57.9%).The pathogen detective sensitivity and accuracy of mNGS were higher than those of conventional methods(Sensitivity:65.6%vs 37.2%;P<0.001;Accuracy:72.0%vs 50%,P<0.001).The results showed that compared with conventional methods,mNGS technology was a more sensitive method for the diagnosis of CNS infection after empirical treatment.CONCLUSION mNGS can be a better method applied in the diagnosis of CNS infection after empirical treatment.

关键词： Metagenomic next-generation sequencing Cerebrospinal fluid Central nervous system infection Pathogenic culture

New insights into the biological roles of immune cells in neural stem cells in post-traumatic injury of the central nervous system

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Neural Regeneration Research 2023年第9期18卷 1908-1916页

作者： Ning He Xing-Jia Mao Yue-Min Ding Tong Zuo ying-ying chen Lin-Lin Wang Department of Basic Medicine Sciences and Department of Orthopaedics of Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina School of Medicine Hangzhou City UniversityHangzhouZhejiang ProvinceChina University of Chicago College University of ChicagoChicagoILUSA Department of Basic Medicine Sciences and Department of Obstetrics of the Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina

Traumatic injuries in the central nervous system,such as traumatic brain injury and spinal cord injury,are associated with tissue inflammation and the infiltration of immune cells,which simultaneously affect the self-renewal and differentiation of neural stem cells.Howeve r,the tissue repair process instigated by endogenous neural stem cells is incapable of restoring central nervous system injuries without external intervention.Recently,resident/peripheral immune cells have been demonstrated to exert significant effects on neural stem cells.Thus,the resto ration of traumatic injuries in the central nervous system by the immune intervention in neural stem cells represents a potential therapeutic method.In this review,we discuss the roles and possible mechanisms of immune cells on the selfrenewal and differentiation of neural stem cells along with the prognosis of central nervous system injuries based on immune intervention.Finally,we discuss remaining research challenges that need to be considered in the future.Further elucidation of these challenges will fa cilitate the successful application of neural stem cells in central nervous system injuries.

关键词： B cells central nervous system injury macrophages microglia neural stem cells spinal cord injury T cells traumatic brain injury

An Azoester-containing Photoresponsive Linear Liquid Crystal Polymer with Good Mesophase Stability

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Chinese Journal of Polymer Science 2020年第8期38卷 806-813,I0005页

作者： Shu-Qiang Han ying-ying chen Bo Xu Jia Wei Yan-Lei Yu Department of Materials Science and State Key Laboratory of Molecular Engineering of Polymers Fudan UniversityShanghai200433China

Photoresponsive linear liquid crystal polymers(LLCPs)are attractive because of the excellent stimuli-responsibility and the good processability.In this study,a new photoresponsive LLCP containing azoester(PC11AE6)with good mesophase stability was synthesized by ringopening metathesis polymerization.By introducing photoresponsive azoester mesogenic unit,which has high rigidity and a large lengthdiameter ratio,the resultant polymer possesses a broad mesophase temperature interval(isotropic temperature=180℃).A study on mesophase by 2D-wide angle X-ray diffraction indicated that the mesogens were orientated spontaneously into smectic A phase after annealing.The orientated films and fibres exhibited macroscopic,rapid and reversible deformations under light irradiation as a result of the photoisomerization of azoester as confirmed by UV-Vis absorption spectrophotometry.We anticipate that this work provides a strategy for preparing LLCP with a broad mesophase temperature range,which is positive for potential applications.

关键词： Ring-opening metathesis polymerization Liquid crystal polymers Azoester Photodeformation

Oral tolerance therapy in type 1 diabetes mellitus

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Chinese Medical Journal 2021年第1期134卷 44-46页

作者： Rui-Feng Mao ying-ying chen En-Jie Diao Xin Chang Zhen-Jing Chi Ye-Fu Wang Jiangsu Collaborative Innovation Center of Regional Modern Agriculture&Environmental Protection School of Life ScienceHuaiyin Normal UniversityHuai’anJiangsu 223300China Department of Ultrasound Medicine Nanjing Lishui People’s HospitalZhongda Hospital Lishui BranchSoutheast UniversityNanjingJiangsu 211200China Huai’an First People’s Hospital Nanjing Medical UniversityHuai’anJiangsu 223300China State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhanHubei 430072China

Type 1 diabetes mellitus(T1DM),an autoimmune disorder,is marked by T cell-mediated destruction of theβcells of the pancreas,which leads to an almost complete loss of the ability to synthesize insulin.[1]This insulin deficiency results in loss of the ability to regulate blood sugar,therefore,exogenous insulin administration is necessary for patients to control blood sugar and to reduce the incidence of related chronic diabetic complications.[2]In addition,a positive correlation between diabetes mellitus and hypertension has been detected.[3]

关键词： mellitus diabetes sugar

Type 1 diabetes mellitus and its oral tolerance therapy

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World Journal of Diabetes 2020年第10期11卷 400-415页

作者： Rui-Feng Mao ying-ying chen Ji Zhang Xin Chang Ye-Fu Wang Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection School of Life ScienceHuaiyin Normal UniversityHuai'an 223300Jiangsu ProvinceChina School of Life Sciences Huaiyin Normal UniversityHuai'an 223300Jiangsu ProvinceChina Department of Ultrasound Medicine Nanjing Lishui People’s HospitalZhongda Hospital Lishui BranchSoutheast UniversityNanjing 211200Jiangsu ProvinceChina College of Life Sciences Wuhan UniversityWuhan 430072Hubei ProvinceChina

As a T cell-mediated autoimmune disease,type 1 diabetes mellitus(T1DM)is marked by insulin defect resulting from the destruction of pancreaticβ-cells.The understanding of various aspects of T1DM,such as its epidemiology,pathobiology,pathogenesis,clinical manifestations,and complications,has been greatly promoted by valuable research performed during the past decades.However,these findings have not been translated into an effective treatment.The ideal treatment should safely repair the destroyed immune balance in a longlasting manner,preventing or stopping the destruction ofβ-cells.As a type of immune hypo-responsiveness to the orally administrated antigen,oral tolerance may be induced by enhancement of regulatory T cells(Tregs)or by anergy/deletion of T cells,depending on the dosage of orally administrated antigen.Acting as an antigen-specific immunotherapy,oral tolerance therapy for T1DM has been mainly performed using animal models and some clinical trials have been completed or are still ongoing.Based on the review of the proposed mechanism of the development of T1DM and oral tolerance,we give a current overview of oral tolerance therapy for T1DM conducted in both animal models and clinical trials.

关键词： Type 1 diabetes mellitus Immunotherapy Oral tolerance Regulatory T cells Antigen-specific immunotherapy

Leaching and mass transfer characteristics of elements from ion-adsorption type rare earth ore

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Rare Metals 2015年第5期34卷 357-365页

作者： Yan-Fei Xiao Zong-Yu Feng Gu-Hua Hu Li Huang Xiao-Wei Huang ying-ying chen Ming-Lai Li National Engineering Research Center for Rare Earth Materials General Research Institute for Nonferrous Metals Grirem Advanced Materials Co. Ltd. Chalco Guangxi Nonferrous Rare Earth Development Co. Ltd. School of Chemical Engineering China University of PetroleumBeijing

The differences in the leaching and mass transfer characteristics of ion-adsorption type rare earth ore between rare earth and non-rare earth impurities were investigated. It is determined that the leaching kinetics of rare earths （REs）, Mg and Ca could be described by the shrinking-core model. The leaching rate is controlled by inner diffusion and is in the order of Mg 〉 Ca 〉 RE. The apparent activation energy is 8.48, 6.79 and 6.32 kJ·mol^-1, respectively. In leaching system of Al, the leaching efficiency decreases with the increase in temperature because of hydrolysis reactions. The leaching efficiency of aluminum increases firstly and then decreases with the passage of time when the temperature is higher than 35 ℃. Besides, there is a good consistency in leaching characteristics and mass transfer, and the order of the time-to- peak is Mg 〈 Ca 〈 RE 〈 Al. Therefore, rare earth and non-rare earth may be partly separated by expanding the separation coefficient. The research could provide a theoretic basis for the separation of rare earth and non-rare earth impurities. Furthermore, compound leaching with magnesium salt and calcium salt is innovatively proposed to solve the problem of calcium and magnesium nutrient loss in the soil as well as eliminate ammonia-nitrogen emissions from the source.

关键词： impurities Rare earth Leaching Non-rare earth Mass transfer

Genome-wide DNA methylation patterns in monocytes derived from patients with primary Sjogren syndrome

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Chinese Medical Journal 2021年第11期134卷 1310-1316页

作者： Xuan Luo Yu Peng ying-ying chen An-Qi Wang Chui-Wen Deng Lin-Yi Peng Qing-Jun Wu Yan Zhao Yun-Yun Fei Wen Zhang Department of Rheumatology and Clinical Immunology Chinese Academy of Medical Sciences&Peking Union Medical CollegeNational Clinical Research Center for Dermatologic and Immunologic Diseases(NCRC-DID)Ministry of Science&TechnologyState Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College Hospital(PUMCH)Key Laboratory of Rheumatology and Clinical ImmunologyMinistry of EducationBeijing 100730China

Background:Epigenetics,especially DNA methylation,plays an important role in the pathogenesis of primary Sjogren syndrome(pSS).Our study aimed to reveal the role of DNA methylation in peripheral monocytes of pSS patients.Methods:A total of 11 pSS patients and five age-matched healthy controls(HCs)were included in this study.Monocytes were isolated from peripheral blood mononuclear cells using magnetic microbeads.DNA methylation profiles were generated using Human Methylation 850K BeadChips.Results:In monocytes from pSS patients,we identified 2819 differentially methylated positions(DMPs),comprising 1977 hypomethylated-and 842 hypermethylated-DMPs,corresponding to 1313 unique genes when compared with HCs.IFI44L,MX1,PAARP9,and IFITM1,which influence the interferon(IFN)signaling pathway,were among the genes hypomethylated in pSS.Functional analysis of genes with a minimum of two DMPs showed involvement in antigen binding,transcriptional regulation,cell adhesion,IFN-g pathway,type I IFN pathway,antigen presentation,Epstein-Barr virus infection,human T-lymphotropic virus type 1 virus infection,and metabolic disease-related pathways.In addition,patients with higher serum IgG levels exhibited enrichment in Notch signaling and metabolic-related pathways.Upon comparing monocytes with salivary gland epithelial cells,an important overlap was observed in the cell cycle,cell senescence,and interleukin-17 signaling pathways.The differentially methylated genes were more enriched in the ribosome-and AMP-activated protein kinase signaling pathway in anti-Ro/SSA and anti-La/SSB autoantibodies double-positive patients.Conclusion:Genome-wide DNA methylation profiling revealed significant differences in DNA methylation in monocytes isolated from patients with pSS.

关键词： Sjogren syndrome Monocyte DNA methylation