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Bacillus subtilis Co-transfected with a Lysine-rich and a Methionine-rich Protein Gene and Its Effect on Cow Milk Production

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Journal of Northeast Agricultural University(English Edition) 2016年第4期23卷 47-54页

作者： Wu Cai-xia Yuan Xiao-han Liu ying Ao Jin-xia Gao Xue-jun Zhang Ming-hui Life Science Research Center Hebei North University Key Laboratory of Dairy Science of Education Ministry Northeast Agricultural University

The probiotic Bacillus subtilis （B. subtilis） was widely applied in animal production as feed additive. Lysine （Lys） and methionine （Met） were the two most important limiting amino acids in livestock animal feed. Raising Lys and Met contents in B. subtilis would provide better effects for animal production and save Lys and Met supplements. We still didn＇t know whether Lys- rich and Met-rich protein genes from plants could be transfected into B. subtilis and expressed at a high level so as to improve animal production, such as milk production as an additional diet. The Lys-rich protein gene （Cflr） and Met-rich 10 ku-δ Zein were cloned from pepper anther and maize endosperm, respectively. Then they were constructed into plasmids individually and successfully cotransfected into B. subtilis. Upon IPTG induction, mRNAs and protein expressions could be observed. Lys and Met contents in the fermentation broth were raised by 65.92% and 46.39%, respectively. After feeding 200 g and 400 g· cow^-1· d^-1, transgenic B. subtilis fermentation broth, the milk yield, milk protein and milk fat contents all significantly increased. The Lys-rich protein gene （Cflr） and Met-rich 10 ku-δ Zein were successfully transfected into B. subtilis. Contents of Lys and Met in the transgenic B. subalis obviously raised and the fermentation broth of the transgenic bacteria could effectively improve milk yield and quality.

关键词： Bacillus subtilis lysine-rich protein gene methionine-rich protein gene cow milk yield milk quality

HDAC6 mutations rescue human tau-induced microtubule defects in Drosophila

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HDAC6 mutations rescue human tau-induced microtubule defects...

Characterization of viromes within mosquito species in China

中国神经科学学会第十届全国学术会议

作者： ying XIONG Kai ZHAO Jia-Xi Wu Zhi-Heng XU shan JIN Yong Q.ZhanG Key Laboratory of Molecular and Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of Sciences College of Life Sciences Hubei University

Neurons from the brains of Alzheimer’s disease（AD） and related tauopathy patients contain neurofibrillary tangles composed of hyperphosphorylated tau *** normally stabilizes microtubules（MTs）;however,tau hyperphosphorylation leads to loss of this function with consequent MT destabilization and neuronal ***,MT-stabilizing drugs such as paclitaxel and epothilone D have been shown as possible therapies for AD and related ***,MT-stabilizing drugs have common side effects such as neuropathy and *** find previously undescribed suppressors of tau-induced MT defects,we established a Drosophila model ectopically expressing human tau in muscle cells,which allow for clear visualization of the MT *** tau was hyperphosphorylated and resulted in decreased MT density and greater fragmentation,consistent with previous reports in AD patients and mouse *** a genetic screen,we found that a histone deacetylase 6（HDAC6） null mutation rescued tau-induced MT defects in bom muscles and *** and pharmacological inhibition of the tubulin speci-fic deacetylase activity of HDAC6 indicates that the rescue effect may be mediated by increased MT *** findings reveal HDAC6 as a unique potential drug target for AD and related tauopathies.

关键词： disease model genetic study

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Science China(Life Sciences) 2020年第7期63卷 1089-1092页

作者： Jiang Du Fan Li Yelin han Shihong Fu Bo Liu Nan Shao Haoxiang Su Weijia Zhang Dandan Zheng Wenwen Lei Jie Dong Lilian Sun ying He Jianmin Wang Fan Yang Huanyu Wang Guodong Liang Zhiqiang Wu Qi Jin NHC Key Laboratory of Systems Biology of Pathogens Institute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Department of Viral Encephalitis NHC Key Laboratory of BiosafetyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China State Key Laboratory of Infectious Disease Prevention and Control Beijing 102206China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003China Key Laboratory of Tropical Translational Medicine of Ministry of Education Hainan Medical UniversityHaikou 571199China

Mosquitoes are vectors of multiple disease-causing agents that cause worldwide outbreaks of serious infectious diseases in both humans and animals. These arboviral agents include Dengue virus (DENV), Zika virus (ZIKV), chikungunya virus, yellow fever virus, Japanese encephalitis virus (JEV),and Rift Valley fever virus. Thus, mosquitoes are key to the study of viral origin, spread, and the epidemic tendency of these infectious diseases (Hall and Macdonald, 2016).

关键词： diseases encephalitis fever

Evolution of a two-mode squeezed vacuum for amplitude decay via continuous-variable entangled state approach

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Frontiers of physics 2018年第5期13卷 231-239页

作者： Xiang-Guo Meng Ji-Suo Wang Bao-Long Liang Cheng-Xuan han School of Physical Science and Information Engineerig Liaocheng University Liaocheng 252059 China shandong Provincial Key Laboratory of Optical Communication Science and Technology Liaocheng University Liaocheng 252059 China shandong Provincial Key Laboratory of Laser Polarization and Information Technology College of Physics and Engineering Qufu Normal University Qufu 273165 China

Extending the recent work completed by Fan et al. [Front. Phys. 9（1）, 74 （2014）] to a two-mode case, we investigate how a two-mode squeezed vacuum evolves when it undergoes a two-mode amplitude dissipative channel, with the same decay rate ~, using the continuous-variable entangled state approach. Our analytical results show that the initial pure-squeezed vacuum state evolves into a definite mixed state with entanglement and squeezing, decaying over time as a result of amplitude decay. We also investigate the time evolutions of the photon number distribution, the Wigner function, and the optical tomogram in this channel. Our results indicate that the evolved photon number distribution is related to Jacobi polynomials, the Wigner function has a standard Gaussian distribution （corresponding to the vacuum） at long periods, losing its nonclassicality due to amplitude decay, and a larger squeezing leads to a longer decay time.

关键词： two-mode squeezed vacuum amplitude decay continuous-variable entangled staterepresentation photon number distribution Wigner function optical tomogram

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恶性疟原虫顶端膜抗原1基因多态性分析

中国寄生虫学与寄生虫病杂志 2014年第5期32卷 344-347页

作者：周银发张山鹰林耀莹杨发柱谢汉国肖方震福建省疾病预防控制中心福州350001

目的研究不同地理分离株恶性疟原虫裂殖子顶端膜抗原1(Pf AMA-1)基因的多态性。方法采集2006-2012年福建省23例输入性恶性疟患者的血样,以血样中的疟原虫DNA为模板,巢式PCR扩增AMA-1基因片段,利用生物软件进行序列比对分析。结果 23份... 详细信息

目的研究不同地理分离株恶性疟原虫裂殖子顶端膜抗原1(Pf AMA-1)基因的多态性。方法采集2006-2012年福建省23例输入性恶性疟患者的血样,以血样中的疟原虫DNA为模板,巢式PCR扩增AMA-1基因片段,利用生物软件进行序列比对分析。结果 23份血样均扩增出目的条带(约505 bp),发现32个多态位点,共计18个单倍型,其中8个为新报道序列。来自非洲的恶性疟原虫分离株的AMA-1基因序列具有较丰富的遗传多样性[单倍型多样度(Hd)=0.985,核苷酸多样度(π)=0.0258],亚洲(东南亚和中国云南)的遗传多样性相对较低(Hd=0.909,π=0.0221)。多样化选择分析结果显示,非同义突变率与同义突变率差值(d N-d S)为0.031±0.006,中性检验结果均无统计学意义(P>0.05)。基因内重组分析显示,重组事件的最低数量(Rm)为10,连锁不平衡指数R2随核苷酸遗传距离增加呈明显下降趋势。采用邻接法构建的分子系统树显示,所有分离株分为3个群(G1,G2和G3),G1多为非洲分离株,G3大部分为亚洲分离株。结论来自非洲的恶性疟原虫分离株的AMA-1基因序列具有较丰富的遗传多样性。

关键词：恶性疟原虫顶端膜抗原1 基因多态性

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表面活性剂对制备石英管薄膜电阻的影响

中南大学学报（自然科学版） 2007年第5期38卷 809-814页

作者：何捍卫黄冠周科朝魏盈盈李瑞迪中南大学粉末冶金国家重点实验室湖南长沙410083

研究在复合电沉积过程中不同表面活性剂对镀层粒子SiO2含量、镀层形貌及电阻率的影响。用扫描电镜、X射线衍射、能谱等手段对镀层表面形貌、微观结构进行检测,并且使用万用电表和SDHC型数字点式测量仪对表面电阻率进行分析测量。实验结... 详细信息

研究在复合电沉积过程中不同表面活性剂对镀层粒子SiO2含量、镀层形貌及电阻率的影响。用扫描电镜、X射线衍射、能谱等手段对镀层表面形貌、微观结构进行检测,并且使用万用电表和SDHC型数字点式测量仪对表面电阻率进行分析测量。实验结果表明,非离子和阳离子表面活性剂的联合使用有利于粒子和基质金属的共沉积,当非离子表面活性剂质量浓度为0.02 g/L,阳离子表面活性剂质量浓度为0.08 g/L时,镀层的微粒含量为3.53%,其电阻率为5.723 kΩ.μm,且表面形貌较好。

关键词：表面活性剂石英管电阻率复合电沉积

A comparative study of biliary trace elements and clinical phenotypes in Wilson disease

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World Journal of Gastroenterology 1997年第4期3卷 62-64页

作者： REN Mingshan1, FAN YuXin2, YANG RenMin1, han YongZhu1, WU GuoJun2, XIN YuRong2 and YU Long2

IM To further explore the etiological mechanism of Wilson disease (WD), by comparing the changes of biliary trace elements and its clinical phenotype.METHODS Wilson disease patients with different types and conditions (n=20), nonWD patients with chronic liver damage (n=22) and healthy volunteers (n=10) as controls were studied. Biliary samples were taken by duodenal drainage. Atom absorption spectrophotometer was used to assay the copper and zinc content of each sample.RESULTS In Wilson disease, the copper content and copper/zinc ratio of biliary juice were evidently lower than those of nonWD patients with chronic liver damage and healthy controls (F=1476, 254; 1492, 262; P005). There were significant differences in biliary copper excretion among patients with different types and conditions (F=375, P<005; F=620, P<001).CONCLUSION Copper excretion by liver and biliary system decreases obviously, which plays a key role in the copper retention of WD, and the biliary copper retention is closely related with the severity of hepatic injury and illness.

关键词： Wilson disease copper zinc duodenal drainage bile

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淋巴结内痣2例临床病理学特征及文献复习

复旦学报（医学版） 2023年第6期50卷 856-861页

作者：韩滢韩春金圣杰朱琪张沁唐峰上海市静安区中心医院病理科上海200040 复旦大学附属华山医院病理科上海200040

目的探讨淋巴结内痣(nodal nevi,NN)的临床病理学特征及鉴别诊断要点。方法收集上海市静安区中心医院2018年至2019年诊断的2例NN患者,回顾性分析其临床病理学特征,行HE染色及免疫组化染色进行观察分析,并复习相关文献。结果患者均为... 详细信息

目的探讨淋巴结内痣(nodal nevi,NN)的临床病理学特征及鉴别诊断要点。方法收集上海市静安区中心医院2018年至2019年诊断的2例NN患者,回顾性分析其临床病理学特征,行HE染色及免疫组化染色进行观察分析,并复习相关文献。结果患者均为女性,年龄分别为48岁和50岁,均无恶性黑色素瘤病史,因乳腺癌行改良根治术后于腋窝淋巴结内发现NN。术后随访44~49个月,2例患者均未见复发及转移。镜下2例NN的痣细胞呈巢团状聚集,分别位于淋巴结被膜处及边缘窦内,病灶最大径分别为2 mm和2.5 mm,痣细胞形态温和,类似于皮肤的良性色素痣细胞,缺乏明显的异型性及核分裂象。免疫组化检测结果示S100均呈强阳性表达,SOX10、P16均呈阳性,Ki67阳性计数均<1%;HMB45、KP-1、CKpan均呈阴性。结论 NN是一种少见的良性淋巴结内黑色素细胞增生性病变,结合临床病史、组织病理学特征、免疫组化及分子检测结果有助于与淋巴结转移性癌及转移性恶性黑色素瘤相鉴别。

关键词：淋巴结内痣(NN) 免疫组化临床病理鉴别诊断预后

京津冀城市群冬季二次PM_(2.5)的时空分布特征

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环境科学 2023年第5期44卷 2421-2429页

作者：姚青杨旭唐颖潇樊文雁蔡子颖韩素芹天津市环境气象中心天津300074 中国气象局-南开大学大气环境与健康研究联合实验室天津300074

二次组分是造成京津冀城市群冬季PM_(2.5)污染的重要因素.采用CO示踪法,估算2017~2021年冬季京津冀城市群二次PM_(2.5)浓度,并分析其时空分布特征,探讨区域二次PM_(2.5)的影响因素.结果表明,2017~2021年冬季京津冀区域PM_(2.5)浓度下降... 详细信息

二次组分是造成京津冀城市群冬季PM_(2.5)污染的重要因素.采用CO示踪法,估算2017~2021年冬季京津冀城市群二次PM_(2.5)浓度,并分析其时空分布特征,探讨区域二次PM_(2.5)的影响因素.结果表明,2017~2021年冬季京津冀区域PM_(2.5)浓度下降趋势明显,河北中南部一次PM_(2.5)下降幅度最大,二次PM_(2.5)浓度年际波动平稳,北京和天津二次PM_(2.5)占比明显高于其他城市.随着污染程度加剧,一次PM_(2.5)和二次PM_(2.5)质量浓度均有不同程度的增加,二次PM_(2.5)占比呈显著增大趋势.与直接测量结果相比,CO示踪法获得的结果偏低,与冬季CO浓度较高,一次PM_(2.5)浓度高估有关,选取合适的一次气溶胶基准值是改进该方法,获取合理估算值的关键.

关键词：二次气溶胶京津冀城市群时空分布光化学反应臭氧(O_(3))

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