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CacyBP/SIP nuclear translocation regulates p27Kip1 stability in gastric cancer cells

World Journal of Gastroenterology 2016年第15期22卷 3992-4001页

作者： ying-Lin niu Ya-Jun Li Jing-Bo Wang Yuan-Yuan Lu Zhen-Xiong Liu Shan-Shan Feng Jian-Guo Hu Hui-Hong Zhai Department of Gastroenterology Beijing Friendship Hospital Affiliated to Capital Medical University Department of Digestive Diseases General Hospital of Ningxia Medical University State Key Laboratory of Cancer Biology Institute of Digestive Diseases Xijing Hospital Fourth Military Medical University

AIM: To investigate the mechanism of calcyclin binding protein/Siah-1 interacting protein(Cacy BP/SIP) nuclear translocation in promoting the proliferation of gastric cancer(GC) cells. METHODS: The effect of Cacy BP/SIP nuclear translocation on cell cycle was investigated by cell cycle analysis. Western blot analysis was used to assess the change in expression of cell cycle regulatory proteins and proteasome-mediated degradation of p27Kip1. Coimmunoprecipitation(co-IP) analysis was performed to examine the binding of Cacy BP/SIP with Skp1. A Cacy BP/SIP truncation mutant which lacked the Skp1 binding site was constructed and fused to a fluorescent protein. Subsequently, the effect on Skp1 binding with the fusion protein was examined by co-IP, while localization of fluorescent fusion protein observed by confocal laser microscopy, and change in p27Kip1protein expression assessed by Western blot ***: Cacy BP/SIP nuclear translocation induced by gastrin promoted progression of GC cells from G1 phase. However, while Cacy BP/SIP nuclear translocation was inhibited using si RNA to suppress Cacy BP/SIP expression, cell cycle was clearly inhibited. Cacy BP/SIP nuclear translocation significantly decreased the level of cell cycle inhibitor p27Kip1, increased Cyclin E protein expression whereas the levels of Skp1, Skp2, and CDK2 were not affected. Upon inhibition of Cacy BP/SIP nuclear translocation, there were no changes in protein levels of p27Kip1 and Cyclin E, while p27Kip1 decrease could be prevented by the proteasome inhibitor MG132. Moreover, Cacy BP/SIP was found to bind to Skp1 by immunoprecipitation, an event that was abolished by mutant Cacy BP/SIP, which also failed to stimulate p27Kip1 degradation, even though the mutant could still translocate into the ***: Cacy BP/SIP nuclear translocation contributes to the proliferation of GC cells, and Cacy BP/SIP exerts this effect, at least in part, by stimulating ubiquitin-mediated degradation o

关键词： Calcyclin binding protein/Siah-1 interacting protein Gastric cancer Cell cycle P27kip1 Ubiquitin

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基于变步长约瑟夫遍历和DNA动态编码的图像加密算法

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电子与信息学报 2020年第6期42卷 1383-1391页

作者：牛莹张勋才郑州轻工业大学建筑环境工程学院郑州450002 郑州轻工业大学电气信息工程学院郑州450002

数字图像传输和存储的安全问题已成为信息安全研究的热点。该文提出一种基于变步长约瑟夫遍历和DNA动态编码的图像加密方法。首先将混沌映射产生的随机序列作为约瑟夫遍历的变步长,改进约瑟夫遍历问题,并采用改进的约瑟夫遍历对图像像... 详细信息

数字图像传输和存储的安全问题已成为信息安全研究的热点。该文提出一种基于变步长约瑟夫遍历和DNA动态编码的图像加密方法。首先将混沌映射产生的随机序列作为约瑟夫遍历的变步长,改进约瑟夫遍历问题,并采用改进的约瑟夫遍历对图像像素位置进行置乱;其次,动态选择DNA编码规则,对图像像素进行DNA编码,并与给定的DNA序列进行碱基运算;DNA编码规则的动态选择,很好地解决了DNA编码规则少所带来的安全隐患,提高了算法的安全性。最后通过密文反馈和混沌系统迭代来进一步增强算法的混淆和扩散特性。实验和安全性分析结果表明,该算法不仅对密钥的敏感性强,而且能有效抵御统计性分析和穷举分析等攻击操作。

关键词：图像加密 DNA动态编码约瑟夫遍历置换

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基于填充曲线和相邻像素比特置乱的图像加密方法

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电子与信息学报 2022年第3期44卷 1137-1146页

作者：牛莹张勋才郑州轻工业大学建筑环境工程学院郑州450002 郑州轻工业大学电气信息工程学院郑州450002

为提高图像传输安全性,该文提出一种基于填充曲线和相邻像素比特置乱的加密算法。首先,设计一种新的填充曲线用于图像像素的全局置乱。其次,将混沌序列作为约瑟夫遍历的起点和步长,利用改进的约瑟夫遍历方法对相邻像素进行比特级置乱。... 详细信息

为提高图像传输安全性,该文提出一种基于填充曲线和相邻像素比特置乱的加密算法。首先,设计一种新的填充曲线用于图像像素的全局置乱。其次,将混沌序列作为约瑟夫遍历的起点和步长,利用改进的约瑟夫遍历方法对相邻像素进行比特级置乱。像素级和比特级的双重置换,打破了图像像素间的高度相关性。最后,通过双向密文反馈,进一步提高方法的安全性。此外,设计了一种与明文图像关联的自适应密钥生成方法,以克服选择/已知明文攻击。并从密钥空间、密钥灵敏度、信息熵和相关性等性能指标对该方案进行了分析,结果表明,该算法具有良好的性能和足够的安全性。

关键词：图像加密填充曲线约瑟夫遍历比特置乱相邻像素

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固体含量对PI/SiO_2纳米杂化薄膜聚集态结构的影响

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化工新型材料 2009年第9期37卷 92-95页

作者：牛颖大庆医学高等专科学校化学教研室大庆163312

以均苯四甲酸二酐（PMDA）和4，4’-二氨基二苯醚（ODA）为有机单体，正硅酸乙酯（TEOS）为无机前驱体，制备了SiO2含量一定，PAA杂化胶液固体含量不同的PI／SiO2纳米杂化薄膜。利用傅立叶变换红外光谱（FT—IR）、原子力显微镜（AFM）... 详细信息

以均苯四甲酸二酐（PMDA）和4，4’-二氨基二苯醚（ODA）为有机单体，正硅酸乙酯（TEOS）为无机前驱体，制备了SiO2含量一定，PAA杂化胶液固体含量不同的PI／SiO2纳米杂化薄膜。利用傅立叶变换红外光谱（FT—IR）、原子力显微镜（AFM）、广角X射线衍射仪（WAXD）分析了PAA杂化胶液固体含量对PI／SiO2纳米杂化薄膜聚集态结构的影响。研究结果表明，当PAA杂化胶液固体含量为10％～20％时，团聚的SiO2粒子粒径尺寸随固体含量增加而增加，有机相与无机相界面变清晰，两相作用减弱。随固体含量增加，PI分子链有序度降低，但固体含量对PI分子链有序度的影响很小。

关键词：聚酰亚胺二氧化硅纳米杂化固体含量聚集态结构

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Immobilizing Triphenylamine with Photoredox Inert Sr^(2+)Forming Sr-MOF with Controlled Electron Migration for Photocatalytic Oxidation of Thiols to Disulfides

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Chinese Journal of Chemistry 2024年第10期42卷 1093-1099页

作者： ying-Xia Wang Fang Zheng Dong-Xia Song Bao-Yue niu Lu-Qian Deng Xian-Ming Zhang Key Laboratory of Magnetic Molecules and Magnetic Information Materials(Ministry of Education) Schoolof Chemistry and Materials ScienceShanxi Normal UniversityTaiyuanShanxi 030006China Key Laboratory of Interface Science and Engineering in Advanced Material(Ministry of Education) College of ChemistryTaiyuan University of TechnologyTaiyuanShanxi 030024China

The photocatalytic oxidative coupling of thiols is one of the most popular methods to synthesize the *** and its derivatives(TPAs)are promising for the above reaction,but suffer from the easy polymerization and difficult *** overcome these obstacles while controlling the photogenerated electrons transfer directly to target substrates,herein,we constructed one TPA-based metal-organic framework(MOF),(Me_(2)NH_(2))[Sr(TCBPA)]·DMA·3H_(2)O(1),by direct self-assembly of tris(4′-carboxybiphenyl)amine(H_(3)TCBPA)and photoredox inert strontium ion(Sr^(2+)).DFT calculations revealed that the valence band maximum(VBM)and the conduction band minimum(CBM)are mainly located on TCBPA^(3-),successfully inhibiting the undesirable electron migration to metal *** results indicated that 1 displays superior performance than homogeneous H_(3)TCBPA,which may result from the abundant π…π and C—H…π interactions between the well-arranged TCBPA^(3-)and the build-in electric field between the anionic framework and the Me_(2)NH^(2+).This work highlights that immobilizing TPAs into MOFs is one promising approach to designing heterogeneous photocatalysts for the synthesis of disulfides by oxidative coupling of thiols.

关键词： Triphenylamine Metal-organic frameworks Strontium Photooxidation Thiols Disulfides

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Flexible piezoelectret film sensor for noncontact mechanical signal capture by multiple transmission media

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Nano Research 2024年第8期17卷 7643-7657页

作者： Xingchen Ma Qianqian Hu Lian Zhou Xinhao Xiang Yi Qin Ke Zhang Pengfei He ying Dai Wenxin niu Xiaoqing Zhang Shanghai Key Laboratory of Special Artificial Microstructure Materials and Technology School of Physics Science and EngineeringTongji UniversityShanghai 200092China Shanghai Yangzhi Rehabilitation Hospital(Shanghai Sunshine Rehabilitation Center) School of MedicineTongji UniversityShanghai 201619China Department of Rehabilitation Sciences Tongji University School of MedicineShanghai 200092China School of Aerospace Engineering and Applied Mechanics Tongji UniversityShanghai 200092China

Mechanical signal capture without physical contact has emerged as a highly promising research field and attracted tremendous attention due to its prosperous applications in household medical care,lifestyle monitoring and remote operation,offering users high level of safety,convenience and ***,noncontact sensing is ideal to maximize the immersive user experience in the human–machine interaction(HMI),eliminating interference to human activities and mechanical fatigue to the sensor,***,we report a self-powered flexible sensor integrated with irradiation cross-linked polypropylene(IXPP)piezoelectret film for noncontact sensing,featuring multi-functions to detect mechanical signals transmitted through solid,liquid and gaseous media and would facilitate their versatile practical *** folded-structure configuration of the sensor facilitates the improvement of the noncontact sensing *** solid media,such as the rectangular wooden stick used in this study,the sensor can detect mechanical stimulus exerted at a distance of 100 cm.A system detection sensitivity up to 57 pC/kPa with a low detection limit of 0.6 kPa is achieved at a noncontact distance of 10 *** when partly or completely immersed in water,the sensor effectively traces movement signals of human bodies underwater,demonstrating great advantages for non-inductive aquatic fitness training ***,due to the low acoustic impedance of piezoelectret film,speech recognition through gaseous medium is also *** further introduce application demonstrations of the developed film sensors to monitor exercise postures and physiological signals without direct contact between human body and the sensor,displaying great potential to be incorporated into future smart *** study commendably expands the application scope of piezoelectret materials,which will have profound implications for exploring novel intelligent human–machine interactions.

关键词： noncontact sensing piezoelectret multiple transmission media mechanical signal capture

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人胚胎视网膜色素上皮细胞在传代中的衰老及其机制

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中华实验眼科杂志 2012年第1期30卷 33-36页

作者：赵颖牛膺筠青岛大学医学院附属市立医院眼科 266011 青岛大学医学院附属医院眼科 266003

背景视网膜色素上皮（RPE）细胞的衰老直接影响视网膜光感受器细胞的代谢功能，导致视网膜功能的异常和视力丧失。深入研究RPE细胞的衰老机制将有助于研究年龄相关性黄斑变性（AMD）的预防和治疗。目的研究人胚胎RPE细胞在传代过程中的... 详细信息

背景视网膜色素上皮（RPE）细胞的衰老直接影响视网膜光感受器细胞的代谢功能，导致视网膜功能的异常和视力丧失。深入研究RPE细胞的衰老机制将有助于研究年龄相关性黄斑变性（AMD）的预防和治疗。目的研究人胚胎RPE细胞在传代过程中的衰老并探讨其机制。方法收集胎龄16～28周的人胎儿眼球6个，对人胚胎RPE细胞进行分离、培养和传代以制作RPE细胞复制性衰老模型，选择3～12代的RPE细胞用于实验。对培养和传代的RPE细胞用免疫组织化学法以人角蛋白对细胞进行鉴定。应用四甲基偶氮唑蓝（MTT）比色法检测各代细胞的增生活力，以RPE细胞的吸光度（A490）表示。采用流式细胞仪检测传代细胞周期和细胞线粒体跨膜电位（△ψm）的变化。结果培养和传代的细胞呈六角形，人角蛋白检测呈棕色，第3代以后的细胞色素消失，3～6代的细胞形态正常，生长活跃，之后生长能力和增生活力下降。培养第3～12代RPE细胞随着传代的增加，处于G0／G1期的细胞百分比逐渐增加，差异有统计学意义（F=180．430，P=0．000），由第3代细胞的68．40％增加到第12代细胞的87．33％，细胞生长趋于停滞。各代间处于G0／G1期细胞的百分比比较，第6代细胞明显高于第3代，第9代明显高于第6代，第12代则明显高于第9代，差异均有统计学意义（t=4．002，P〈0．05；t=12．885，P〈0．01；t=16．387，P〈0．01）。MTT法对RPE细胞活力的检测结果表明，随着传代次数的增多，反映RPE细胞活力的A490值逐渐下降，总体比较差异有统计学意义（F=38．770，P=0．000），第9代、第12代RPE细胞的A490值分别明显低于第6代和第9代，差异均有统计学意义（t=5．991、11．983，P〈0．01）。同时，3—12代PRE细胞随着传代的增加，罗丹明123染色荧光强度逐渐降低，差异有统计学意义（F=121．680，P=0．000），第6、9、12代细胞的平均荧光强度分别低于第3、6、9代，差异均有统计学意义（t=6．918、7．620、11．207，P〈0．01）。结论成功制作了RPE细胞随年龄而逐渐衰老的模型，提示衰老细胞线粒体膜电位降低，线粒体功能受损可能是RPE细胞衰老的机制之一。

关键词：视网膜/色素上皮细胞衰老细胞周期线粒体膜电位

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Assembly and Immunogenicity of Human Papillomavirus Type 16 Major Capsid Protein(HPV16 L1) in Pichia pastoris

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Chemical Research in Chinese Universities 2007年第2期23卷 200-203页

作者： LIU Da-wei ZHANG Yu YU Xiang-hui JIANG Chun-lai CHEN Yue WU Yong-ge JIN ying-hua niu Jun Qu Ning LIU Ming KONG Wei Vaccine Research Center College of Life Science Jilin University Changchun 130023 P. R. China

In this study, a recombinant Pichia pastoris expression system was developed to express HPV16 L1 protein that was driven by a strong AOX1 promoter. HPV16L1 gene was cloned into vector pPICZ,αB. HPV16 L1 protein expression induced by methanol was screened by using sodium dedecyl sulfate-polyacrylamide gel electrophoresis （SDSPAGE） and Western blotting. The results indicate that the HPVl6 L1 protein is secreted by the recombinant P. pastoris, and the purified HPV16 L1 protein can self-assemble into vires-like particles（ VLPs）, which show a good immunogenicity and induces high-titer antibody in mice.

关键词： Human papillomavirus Major capsid protein Recombinant Pichia pastoris Vires-like particles

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Hepatic veins anatomy and piggy-back liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2012年第4期11卷 429-433页

作者： ying-Zi Ming,ying niu,Ming-Jie Shao,Xing-Guo She and Qi-Fa Ye Research Center of Chinese Health Ministry on Transplantation Medicine Engineering and Technology,The Third Xiangya Hospital,Central South University,Changsha 410013,China Research Center of Chinese Health Ministry on Transplantation Medicine Engineering and Technology The Third Xiangya Hospital Central South University Changsha 410013 China

BACKGROUND:The piggy-back caval anastomosis technique is widely used in orthotopic liver transplantation although it carries an increased risk of complications,including outflow obstruction and Budd-Chiari *** aim of this study is to clarify the anatomy and variations of hepatic veins(HVs) draining into the inferior vena cava(IVC),and to classify the surgical techniques of piggy-back liver transplantation(PBLT) based on the anatomy of HVs which can reduce the occurrence of ***:PBLT was performed in 248 consecutive cases at our hospital from January 2004 to August *** anatomy of recipients’ HVs was determined when removing the native diseased *** anatomy of HVs and short HVs draining into the IVC were *** data were collected and ***:We classified anatomic variations of HVs in the 248 livers into five types according to the way of drainage into the IVC:type I(trunk type of left and middle HVs),142(57.3%) patients;type II(trunk type of right and middle HVs),54(21.8%);type III(trunk type of left,middle and right HVs),14(5.6%);type IV(non-trunk type of left,middle and right HVs),of which,type IVa,16(6.5%),in the same horizontal plane;type IVb,18(7.3%),in different horizontal planes;and type V(segment type),4(1.6%).The patients whose HVs anatomy belonged to types I,II and III underwent classical piggy-back liver *** IVa patients had classical PBLT via HV venoplasty prior to piggy-back anastomosis,while type IVb patients and type V patients could only have modified ***:This study demonstrates that HVs can be classified according to the anatomy of their drainage into the IVC and we can use this classification to choose the best operative approach to PBLT.

关键词： hepatic vein classification piggy-back liver transplantation

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西安市小寨区域海绵城市建设项目技术总结

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给水排水 2020年第S1期46卷 613-618页

作者：牛樱北京市市政工程设计研究院有限公司广东分院广东510000

以解决小寨区域水安全方面的突出问题为主线,统筹考虑繁华城区水环境、水生态、水资源等多重问题,工程借助InfoWorks ICM水力模型对区域内涝问题进行了成因分析和方案建议;同时结合现场调研和城市建设规划,提出"LID低影响开发雨水系统+... 详细信息

以解决小寨区域水安全方面的突出问题为主线,统筹考虑繁华城区水环境、水生态、水资源等多重问题,工程借助InfoWorks ICM水力模型对区域内涝问题进行了成因分析和方案建议;同时结合现场调研和城市建设规划,提出"LID低影响开发雨水系统+超标雨水径流控制系统+径流污染控制系统+海绵城市智慧运维管控系统"四大系统建设方案。预期效果评估显示,建设方案可综合实现区域内涝整治、径流控制、污染削减、智慧管控等多项目标,为繁华城区海绵城市建设提供借鉴和参考。

关键词：海绵城市内涝防治分散式海绵调蓄系统 InfoWorks ICM 水力模型

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