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Agricultural Sciences in China 2009年第11期8卷 1351-1359页

作者： ZHAO ying liu na NIU Jian-xin Horticultural Department Agricultural College Shihezi University Shihezi 832003 P.R. China

To understand the distribution of Apple stem pitting virus （ASPV） in tissues of pear tree and provide directly theorical and technical support for shoot tips detoxication, leaves and shoot tips of Korla pear were used as the materials, cDna probe for ASPV was synthesized through RT-PCR reaction system using the unradioactive digoxigenin-labeled probe, and the specificity and sensitivity of probe were verified by blot hybridization method. Paraffin slice for in situ PCR and in situ hybridization was made and the location and distribution of ASPV Rna were detected in paraffin slices using in situ reverse transcription polymerase chain reaction, and the important factors which influenced the experiment results were optimized. ASPV mainly distributed in palisade tissue of mesophyll cells, external cortex of the tip, and the corresponding newborn vascular bundles. 20 min was the suitable digestive time for proteinase K. For the better amplication, RT reaction system should be above 0.2 U μL^-1 and 0.4 mmol L^-1 for Rnasin and dNTPs respectively, 0.1-1.3 U μ L^-1 SuperScript Ⅱ, 0.6- 0.8 μmol L^-1 primer concentration, and above 0.5 U 100 μL^-1 LA Taq Dna polymerase. The suitable annealing temperature in PCR reaction was 60℃ with 35 cycles. The apical meristem of 0.25 mm was the region of virus-free.

关键词： Korla pear Apple stem pitting virus cDna probe digoxigenin in situ PCR

Preparation of Bio-beads and Their Atrazine Degradation Characteristics

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Chemical Research in Chinese Universities 2011年第3期27卷 407-412页

作者： BI Hai-tao ZHANG Lan-ying liu na ZHU Bo-lin College of Environment and Resources Jilin University Changchun 130026 P. R. China

Screened atrazine-mineralizing bacterium―Pseudomonas W4 was embedded inside an improved PVA- H3BO3 embedment matrix to make bio-beads to degrade atrazine. The atrazine degradation characteristics were studied. The preparation procedure of bio-beads was as follows：（1） preparing a mixture of 100, 12.5, 10, 1.5 and 1 g/L PVA, bentonite（Ca）, activated carbon powder, sodium alginate and centrifuged Pseudomonas W4 bacterium, respectively; （2） the mixture was dropped into a gently stirred cross linker solution（pH=6.7） and cured at 10 °C for 24 h. The optimal atrazine degradation conditions by bio-beads were as follows： pH=7, the auxiliary carbon source was glucose, and the concentration of glucose was greater than 325 mg/L. The bio-beads demonstrated stronger tolerance ability than the free microorganism to the increase of PCBs, hydrogen ion and hydroxide ion. SEM images show the uniform distribution of the microorganism inside bio-beads and the porous cross-linked structure of bio-beads which provides excellent mass transfer capacity.

关键词： Atrazine Polyvinyl alcohol(PVA) Embedment Bio-bead

Bibliometric analysis of glaucoma-related literature based on SCIE database:a 10-year literature analysis from 2009 to 2018

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International Journal of Ophthalmology(English edition) 2020年第12期13卷 1998-2006页

作者： Yun-Xiao Sun ying-na liu ying Han Fan-Qi Kong Yue Zhang Siloka Arthur Labisi Kai Cao Beijing Ophthalmology and Visual Sciences Key Laboratory Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijingChina Beijing Institute of Ophthalmology Beijing Tongren HospitalCapital Medical UniversityBeijingChina Department of Ophthalmology University of California San FranciscoSan FranciscoCA 94143USA Allergan plc ShanghaiChina

AIM:To explore the global trends and focus of glaucoma research from 2009 to ***:Searching for glaucoma-related articles published in Science Citation Index Expanded(SCIE)database during 2009-2018,and describing the distribution of the published year,countries,authors,institutions,funding agencies,journals,impact factor,citation and hot research topic of articles by using bibliometric ***,we compared some of these indicators over two five-year periods,from 2009 to 2013 and from 2014 to ***:A total of 19609 glaucoma-related articles were retrieved and the global SCIE articles have increased yearly from 2009 to *** USA was the pioneer which has made great *** kept the second place and the number of publications has increased rapidly between 2014 and *** author with the highest number of publications was Weinreb,***-occurrence maps were built amongst the top 50 authors or the top 50 institutions with the most articles,which visualize the closer collaboration of international authors or *** journal Investigative Ophthalmology&Visual Science has published the most *** literature with an impact factor of 3-5 points accounted for the largest proportion(28.96%).The most frequently cited paper had 798 *** top three hot areas on glaucoma were intraocular pressure,optical coherence tomography(OCT)and retinal ganglion *** trabecular meshwork,primary angle-closure glaucoma and Spectral-domain OCT have become new hot research topics in recent five years during ***:Bibliometric analysis is an effective method to describe the global literature on *** a 10-year literature survey from 2009 to 2018,global glaucoma research has developed in a balanced manner,and the cooperation between various institutions and teams has become ***-related pathogenesis research,imaging examinations of OCT and surgery therapy have attracted most attention.

关键词： bibliometric analysis Science Citation Index Expanded glaucoma ocular hypertension

Maximizing biomass utilization: An integrated strategy for coproducing multiple chemicals

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Journal of Energy Chemistry 2025年第1期100卷 180-191页

作者： Byeongchan Ahn Sua Jo Jonggeol na J.Jay liu Young-Ju Kim Wangyun Won Department of Chemical and Biological Engineering Korea University Department of Chemical Engineering (Integrated process) Kyung Hee University Department of Chemical Engineering and Materials Science Ewha Womans University Department of Chemical Engineering Pukyong National University Resources Engineering Plant Research Department Korea Institute of Geoscience and Mineral Resources

Lignocellulosic biomass is one of the viable solutions to alleviate the global warming. However, the limited utilization of biomass majorly focused on cellulose and hemicellulose restricts the economic and environmental feasibilities. To cope with this issue, we proposed an integrated process of co-producing 1,6-hexanediol（1,6-HDO） with tetrahydrofuran and adipic acid from biomass, referred to as Strategy A. To compare the impacts of lignin upgrading and feedstock, Strategy B, which co-produces tetrahydrofuran alone, and Strategy C, which is the traditional route to produce 1,6-HDO from fossil fuels, were used. Heat networks are also designed to reduce operating costs and indirect carbon emissions due to energy consumption, saving 87% and 83% of the heat and cooling requirements, respectively, in Strategy A. The market competitiveness of Strategy A was evaluated by determining the minimum selling price through techno-economic analysis, and sustainability was thoroughly investigated by quantifying the environmental impacts through both midpoint and endpoint life-cycle assessments（LCAs）.Strategy A was found to be the most favorable both economically（USRDSCHARDOLLAR3,402/ton） and environmentally（-26.9 kg CO2eq.）. This indicates that lignin valorization is not only economically but also environmentally preferred. Finally, changes in economic and environmental feasibilities depending on economic, process, and environmental parameters were investigated using sensitivity and uncertainty analyses. The results of these analyses provide valuable insight into bio-based chemical production.

关键词： Biorefinery Carbon mitigation Climate change Global warming Lignin utilization Bioeconomy

2012年夏季白令海峡附近海域温盐分布特征分析

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海洋科学进展 2017年第1期35卷 40-52页

作者：王颖杰刘娜林丽娜陈红霞国家海洋局第一海洋研究所山东青岛266061

利用中国第五次北极科学考察CTD资料,分析了2012年夏季包括白令海陆架区和楚科奇海在内的白令海峡附近海域水文特征:1)白令海峡南侧的白令海陆架区按照不同的水团性质,可以分为圣劳伦斯岛以南和圣劳伦斯岛以北两个区域。圣劳伦斯岛以南... 详细信息

利用中国第五次北极科学考察CTD资料,分析了2012年夏季包括白令海陆架区和楚科奇海在内的白令海峡附近海域水文特征:1)白令海峡南侧的白令海陆架区按照不同的水团性质,可以分为圣劳伦斯岛以南和圣劳伦斯岛以北两个区域。圣劳伦斯岛以南海域,东部有阿拉斯加沿岸水流过,水体垂向混合均匀,具有高温低盐的分布特征。向西在25m深度附近存在温跃层,跃层以下温度分布出现层化现象。西南部陆架区存在低温的白令海陆架水,其下方靠近白令海陆坡区存在高盐的白令海陆坡流水。圣劳伦斯岛以北海域,东部是高温低盐的阿拉斯加沿岸水,西部是低温高盐的阿纳德尔水。2)白令海峡北侧,入流水对楚科奇海环流影响较大,高温低盐的阿拉斯加沿岸水仍沿东侧近岸海域向北运动。西侧是低温高盐的阿纳德尔水,但至69°00′N附近,该低温高盐信号消失,断面层化现象逐渐显著。随着水团继续向北运动,温度逐渐降低,盐度逐渐升高。

关键词：白令海峡楚科奇海中国第五次北极科学考察温盐特征分析

Flexible molecules dedicate to release strain of inverted inorganic perovskite solar cell

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Journal of Energy Chemistry 2025年第1期100卷 87-93页

作者： Hongrui Sun Sanlong Wang Pengyang Wang Yali liu Shanshan Qi Biao Shi ying Zhao Xiaodan Zhang Institute of Photoelectronic Thin Film Devices and Technology Renewable Energy Conversion and Storage Center State Key Laboratory of Photovoltaic Materials and CellsNankai University Key Laboratory of Photoelectronic Thin Film Devices and Technology of Tianjin Haihe Laboratory of Sustainable Chemical Transformations Engineering Research Center of Thin Film Photoelectronic Technology of Ministry of Education Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)

The tensile strain in inorganic perovskite films induced by thermal annealing is one of the primary factors contributing to the inefficiency and instability of inorganic perovskite solar cells （IPSCs）,which reduces the defect formation ***,a flexible molecule 5-maleimidovaleric acid （5-MVA） was introduced as a strain buffer to release the residual strain of *** anhydride and carboxyl groups in 5-MVA interact strongly with the uncoordinated Pb2+through Lewis acid-base reaction,thus tightly“pull”the perovskite *** in-between soft carbon chain increased the structural flexibility of CsPbI2.85Br0.15perovskite materials,which effectively relieved the intrinsic internal strain of CsPbI2.85Br0.15,resisted the corrosion of external strain,and also reduced the formation of defects such as VIand *** addition,the introduction of 5-MVA improved crystal quality,passivated residual defects,and narrowed energy level ***,power conversion efficiency （PCE） of NiOxbased inverted IPSCs increased from 19.25% to 20.82% with the open-circuit voltage enhanced from 1.164 V to 1.230 *** release of strain also improved the stability of CsPbI2.85Br0.15perovskite films and devices.

关键词： Inverted inorganic perovskite solar cells Flexible molecules Strain release Crystallization Energy barrier High PCE

Observation of topological charge transformations in acoustic vortex using passive periodic systems

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 140-147页

作者： Di-Chao Chen Xie liu Changqing Xu Da-Jian Wu ying Cheng Xiao-Jun liu School of Physics and Technology Nanjing Normal University Key Laboratory of Modern Acoustics School of Physics Nanjing University

Vortex transmutation utilizes a periodic system, which not only enables the alteration of the topological charge(TC) of unstable input vortices but also facilitates their transformation into stable structures. This approach is crucial for investigating the characteristics of vortices in various wave systems. Here, we achieve acoustic vortex TC transmutation by employing a welldesigned dual-structured plate external system(DPES). The interactions between the acoustic vortex and the finite-order discrete rotational symmetry lead to the transformation of its topological charge when complete rotational symmetry is broken to Nthorder discrete symmetry. Our results experimentally demonstrate the acoustic vortex TC transmutation based on passive artificial structures, enabling numerous applications in particle manipulation and information multiplexing as well as advancing our understanding and control over free-space acoustic vortices.

关键词： acoustic vortex topological charge transformation artificial structure

NOX4 exacerbates Parkinson's disease pathology by promoting neuronal ferroptosis and neuroinflammation

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Neural Regeneration Research 2025年第7期20卷 2038-2052页

作者： Zhihao Lin Changzhou ying Xiaoli Si naijia Xue Yi liu Ran Zheng ying Chen Jiali Pu Baorong Zhang Department of Neurology The Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina

Parkinson's disease is primarily caused by the loss of dopaminergic neurons in the substantia nigra ***,a novel form of regulated cell death characterized by iron accumulation and lipid peroxidation,plays a vital role in the death of dopaminergic ***,the molecular mechanisms underlying ferroptosis in dopaminergic neurons have not yet been completely *** oxidase 4 is related to oxidative stress,however,whether it regulates dopaminergic neuronal ferroptosis remains *** aim of this study was to determine whether naDPH oxidase 4 is involved in dopaminergic neuronal ferroptosis,and if so,by what *** found that the transcriptional regulator activating transcription factor 3 increased naDPH oxidase 4 expression in dopaminergic neurons and astrocytes in an 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced Parkinson's disease *** oxidase 4 inhibition improved the behavioral impairments observed in the Parkinson's disease model animals and reduced the death of dopaminergic ***,naDPH oxidase 4 inhibition reduced lipid peroxidation and iron accumulation in the substantia nigra of the Parkinson's disease model ***,we found that naDPH oxidase 4 interacted with activated protein kinase Cαto prevent ferroptosis of dopaminergic ***,by lowering the astrocytic lipocalin-2 expression,naDPH oxidase 4 inhibition reduced 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced *** findings demonstrate that naDPH oxidase 4 promotes ferroptosis of dopaminergic neurons and neuroinflammation,which contribute to dopaminergic neuron death,suggesting that naDPH oxidase 4 is a possible therapeutic target for Parkinson's disease.

关键词： dopaminergic neuron ferroptosis naDPH oxidase 4(NOX4) neuroinflammation Parkinson's disease

Multi boron-doping effects in hard carbon toward enhanced sodium ion storage

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Journal of Energy Chemistry 2025年第1期100卷 730-738页

作者： Peng Zheng Wang Zhou ying Mo Biao Zheng Miaomiao Han Qin Zhong Wenwen Yang Peng Gao Lezhi Yang Jilei liu College of Materials Science and Engineering Hunan Joint International Laboratory of Advanced Materials and Technology for Clean Energy Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology Hunan University Greater Bay Area Institute for Innovation Hunan University Changsha Research Institute of Mining & Metallurgy Co. LTD.

Hard carbon （HC） has been considered as promising anode material for sodium-ion batteries （SIBs）.The optimization of hard carbon’s microstructure and solid electrolyte interface （SEI） property are demonstrated effective in enhancing the na+storage capability,however,a one-step regulation strategy to achieve simultaneous multi-scale structures optimization is highly ***,we have systematically investigated the effects of boron doping on hard carbon’s microstructure and interface chemistry.A variety of structure characterizations show that appropriate amount of boron doping can increase the size of closed pores via rearrangement of carbon layers with improved graphitization degree,which provides more na+storage ***-situ Fourier transform infrared spectroscopy/electrochemical impedance spectroscopy （FTIR/EIS） and X-ray photoelectron spectroscopy （XPS） analysis demonstrate the presence of more BC3and less B–C–O structures that result in enhanced ion diffusion kinetics and the formation of inorganic rich and robust SEI,which leads to facilitated charge transfer and excellent rate *** a result,the hard carbon anode with optimized boron doping content exhibits enhanced rate and cycling *** general,this work unravels the critical role of boron doping in optimizing the pore structure,interface chemistry and diffusion kinetics of hard carbon,which enables rational design of sodium-ion battery anode with enhanced na+storage performance.

关键词： X ray photoelectron spectroscopy

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活塞式菱形结构光纤布喇格光栅渗压传感器

光子学报 2017年第2期46卷 52-58页

作者：谢涛孙恩钊李川赵振刚李英娜刘爱莲张长胜昆明理工大学信息工程与自动化学院昆明650500

为了适应矿井、大坝对渗压监测的需要,提出一种活塞和菱形传压结构相结合的光纤布喇格光栅渗压传感器,活塞把测试压力传递到菱形传压结构,菱形传压结构拉动其上下对称连接的弹性钢片,导致粘贴在弹性钢片上的光纤布喇格光栅中心波长产生... 详细信息

为了适应矿井、大坝对渗压监测的需要,提出一种活塞和菱形传压结构相结合的光纤布喇格光栅渗压传感器,活塞把测试压力传递到菱形传压结构,菱形传压结构拉动其上下对称连接的弹性钢片,导致粘贴在弹性钢片上的光纤布喇格光栅中心波长产生变化.利用有限元方法在500kPa的测试环境中对顶角分别为90°、110°、130°、150°情况下菱形传压结构的应力特性进行分析,根据仿真参量研制了渗压传感器,并对该传感器进行了压力标定试验和温度补偿试验.实验结果表明:传感器对渗压的灵敏度为2.04nm/MPa,拟合度为0.997,重复性为0.9%,两个测压光栅温度灵敏度分别为0.023 33nm/℃、0.021 68nm/℃,温补光栅为0.009 916nm/℃.

关键词：光纤布喇格光栅渗压传感器菱形结构活塞式有限元分析