检索结果-南通市图书馆

Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury

引用

Neural Regeneration Research 2025年第11期20卷 3287-3301页

作者： Ziwei Fan Mengxian Jia Jian Zhou Zhoule Zhu Yumin Wu Xiaowu Lin Yiming Qian Jiashu Lian Xin hua Jianhong Dong Zheyu Fang Yuqing Liu Sibing Chen Xiumin Xue Juanqing yue Minyu Zhu ying Wang Zhihui huang Honglin Teng Department of Orthopedics(Spine Surgery) the First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina School of Pharmacy Hangzhou Normal UniversityHangzhouZhejiang ProvinceChina Department of Neurology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Pathology Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhouZhejiang ProvinceChina Department of Clinical Research Center Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhouZhejiang ProvinceChina

The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord *** of shifting microglia polarization from M1(neurotoxic and proinflammatory type)to M2(neuroprotective and anti-inflammatory type)after spinal cord injury appears to be *** possesses an anti-inflammatory biological ***,its roles and the underlying molecular mechanisms in spinal cord injury remain *** this study,we found that tryptanthrin inhibited microglia-derived inflammation by promoting polarization to the M2 phenotype in *** promoted M2 polarization through inactivating the cGAS/STING/NF-κB ***,we found that targeting the cGAS/STING/NF-κB pathway with tryptanthrin shifted microglia from the M1 to M2 phenotype after spinal cord injury,inhibited neuronal loss,and promoted tissue repair and functional recovery in a mouse model of spinal cord ***,using a conditional co-culture system,we found that microglia treated with tryptanthrin suppressed endoplasmic reticulum stress-related neuronal *** together,these results suggest that by targeting the cGAS/STING/NF-κB axis,tryptanthrin attenuates microglia-derived neuroinflammation and promotes functional recovery after spinal cord injury through shifting microglia polarization to the M2 phenotype.

关键词： cGAS/STING functional recovery microglia neuroinflammation neuroprotection nuclear factor-κB polarization spinal cord injury tryptanthrin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Advances in ethnopharmacology,phytochemistry and pharmacology of Erigerontis Herba and its Chinese medicine prescriptions

引用

Traditional Medicine Research 2025年第1期10卷 44-61页

作者： Yang Zhou Yuan-Yan huang Ding-yue Shang ying Chen Meng Tian Cheng-Yu Jin Xin Lu Bing-Chun Yan Han-Qing Pang Institute of Translational Medicine School of MedicineYangzhou UniversityYangzhou 225009China Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou UniversityYangzhou 225009China School of Medicine Nanjing University of Chinese MedicineNanjing 210023China State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources Guangxi Normal UniversityGuiling 541004China.

Erigerontis Herba(EH),the dried whole plant of Erigeron breviscapus,is well-known for circulating blood,activating meridians to alleviate pain,expelling wind,and clearing away *** has been extensively utilized in southern China for the treatment of stroke hemiplegia,chest stuffiness and pains,rheumatic arthralgia,headache,and *** review focuses on the botany,ethnopharmacology,phytochemistry,pharmacology and toxicity of EH and its related prescriptions to offer new insights for prospective research of *** information about EH was retrieved from ancient records and books,PubMed,China National Knowledge Infrastructure,Chinese Pharmacopoeia,Web of Science,Doctoral and Master’s Theses,and various electronic *** is a member of Compositae family and is mainly grown in southern *** Chinese medicine records that EH has the effects of circulating blood and removing blood stasis,expelling wind,and removing cold,as well as relieving rigidity of muscle and relieving *** now,nearly 200 ingredients have been characterized from EH,including flavonoids,caffeoyls,aromatic acids,coumarins,pentacyclic terpenoids,volatile oil and other *** extracts,EH related prescriptions(Dengzhan Xixin injection,Dengzhan Shengmai capsules,etc.)or compounds(scutellarin,scutellarein,etc.)possessed obvious therapeutic effects of ischemic stroke,cerebral hemorrhage,myocardial infarction,Alzheimer’s disease,diabetes and its complications,gastric cancer,bone,and joint degenerative ***,the major active compound of EH,has been used as a quality *** no obvious toxicity of EH has been *** to its traditional applications,ethnopharmacology,phytochemistry,pharmacology,and toxicity,EH was applied as a valuable herb for clinical application in food and medicine *** several compounds have been shown to possess diverse biological activities,the underlying mechanisms of their actions remain *** fully exploi

关键词： Erigeron breviscapus traditional applications ethnopharmacology phytochemistry pharmacology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Knockdown of STAT3 by iRNA Inhibiting Migration and Invasion of Epithelial Ovarian Cancer Cells

引用

Chemical Research in Chinese Universities 2012年第5期28卷 856-861页

作者： LI Qin-hua ZHU Ji-hong LIU Lei yue ying Department of Gynecology the First Hospital of Jilin UniversityChangchun 130021P.R.China Department of Gastroenterology the First Hospital of Jilin UniversityChangchun 130021P.R.China

Signal transducer and activator of transcription 3（STAT3） is a dual functional transcription factor with the functions of signal transduction and transcription regulation. It is reported that the expression of STAT3 in ovarian cancer is significantly higher and STAT3 can facilitate ovarian cancer growth and metastasis. To clarify the definite effect and molecular mechanism of STAT3 involved in ovarian cancer growth and metastasis, STAT3 expression was significantly downregulated by transfecting ovarian cancer model SK-OV-3 cells with the plasmid vector which express specific RNAi that targets human STAT3. The downregulated STAT3 not only decreased the invasion and migration but also inhibited the proliferation of SK-OV-3 cells. Western blot assay shows that the expression of vascular endothelial growth factor（VEGF） and that of Survivin were reduced in the cells with the plasma vector expressing specific RNAi that targets human STAT3. These results demonstrate that STAT3 involved in the invasion and migration of SK-OV-3 regulates the expression of VEGF and Survivin. In addition, VEGF and Survivin could play an important role in ovarian cancer growth and metastasis.

关键词： Ovarian cancel model SK-OV-3 cell Signal transducer and activator of transcription 3(STAT3) Migration Vascular endothelial growth factor(VEGF) Survivin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

青蒿鞣质抗病毒活性研究

引用

天然产物研究与开发 2004年第4期16卷 307-311页

作者：张军峰谭健蒲蔷刘颖华刘月雪何开泽中国科学院成都生物研究所成都610041

从青蒿水提物中分离得到一种缩合鞣质 (CTA) ,在体外进行了抗HSV 2和HBV活性研究。结果表明 ,CTA具有显著抗HSV 2活性 ,与阳性对照药物阿昔洛韦 (ACV)比较 ,CC50 分别为 6 .84mg/mL和 3.6 9mg/mL ,IC50 分别为 0 .16 2mg/mL和 0 .138mg/... 详细信息

从青蒿水提物中分离得到一种缩合鞣质 (CTA) ,在体外进行了抗HSV 2和HBV活性研究。结果表明 ,CTA具有显著抗HSV 2活性 ,与阳性对照药物阿昔洛韦 (ACV)比较 ,CC50 分别为 6 .84mg/mL和 3.6 9mg/mL ,IC50 分别为 0 .16 2mg/mL和 0 .138mg/mL ,表明CTA的细胞毒性比ACV小 ,最低抗病毒活性浓度相当。用含CTA的培养基培养HepG2 .2 .1.5细胞 ,结果表明在浓度为 2 .5mg/mL时有轻微细胞毒性 ,在浓度为 2 .5～ 0 .15 6mg/mL对HepG2 .2 .1.5细胞表达的HBeAg具有显著的抑制作用 ,0 .6 2 5mg/mL浓度档培养第 12d对HBeAg抑制率达到 90 .4 5 % 。

关键词：缩合鞣质细胞毒性 CTA 阿昔洛韦抗病毒活性 HBV 乙型肝炎病毒

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Etoposide Induces Mitochondria-Associated Apoptotic Cell Death in Human Gastric Carcinoma Cells

引用

Chemical Research in Chinese Universities 2008年第5期24卷 597-602页

作者： LI Jing-hua CHEN yue WANG Jia-si KONG Wei JIN ying-hua Key Laboratory for Molecular Enzymology and Engineering Ministry of Education College of Life Science School of Public Health Jilin University Changchun 130012 P R. China

Recent observations indicate that the resistance of apoptosis is an important process of tumor metastasis and metastases are the cause of 90% of human cancer death. Etoposide, a semisynthetic derivative of the podophyllotoxins, is a clinically used anti-cancer reagent, but the effects of it on metastatic gastric carcinoma cells are totally unknown. In this study, etoposide induced apoptotic cell death in human gastric adenocarcinoma cell line SGC-7901, derived from metastatic lymph nodes, as evidenced by the analysis of DNA fragmentation, apoptotic body formation, caspase activation, and apoptosis specific changes in cell morphology is demonstrated. The depolarization of mitochondrial membrane and the release of cytochrome c were most early events in etoposide treated SGC-7901 cells, and were followed by caspase-3 activation and PARP cleavage. Caspase-8 activation was not detected under the same condition. Thus, it was proposed that etoposide induces caspase-associated apoptotic cell death in human metastatic gastric carcinoma, which is initiated by mitochondrial cytochrome c release.

关键词： Apoptosis Caspase Cytochrome c Etoposide SGC-7901 cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ramulus Cinnamomi extract attenuates neuroinflammatory responses via downregulating TLR4/MyD88 signaling pathway in BV2 cells

引用

Neural Regeneration Research 2017年第11期12卷 1860-1864页

作者： huan Yang Xiao Cheng ying-lin Yang yue-hua Wang Guan-hua Du Beijing Key Laboratory of Drug Target Identification and Drug Screening Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingChina State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingChina

Ramulus Cinnamomi （RC）, a traditional Chinese herb, has been used to attenuate inflammatory responses. The purpose of this study was to investigate the effect of RC extract on lipopolysaccharide （LPS）-induced neuroinflammation in BV2 microglial cells and the underlying mechanisms involved. BV2 cells were incubated with normal medium （control group）, LPS, LPS plus 30 pg/mL RC extract, or LPS plus 100 pg/mL RC extract. The BV2 cell morphology was observed under an optical microscope and cell viability was detected by MTT assay. Nitric oxide level in BV2 cells was detected using Griess regents, and the levels of interleukin-6, interleukin-1 β, and tumor necrosis factor u in BV2 cells were determined by ELISA. The expression levels of cyclooxygenase-2, Toll-like receptor 4 and myeloid differentiation factor 88 proteins were detected by western blot assay. Compared with the LPS group, both 30 and 100 μg/mL RC extract had no significant effect on the viability of BV2 cells. The levels of nitric oxide, interleukin-6, interleukin-1β and tumor necrosis factor ct in BV2 cells were all significantly increased after LPS induction, and the levels were significantly reversed after treatment with 30 and 100 μg/mL RC extract. Furthermore, RC extract significantly inhibited the protein expression levels of cyclooxygenase-2, Toll-like receptor 4 and myeloid differentiation factor 88 in LPS-induced BV2 cells. Our findings suggest that RC extract alleviates neuroinflammation by downregulating the TLR4/MyD88 signaling pathway.

关键词： nerve regeneration Ramulus Cinnamomi BV2 cells lipopolysaccharide neuroinflammation pro-inflammatory factors TLR4/ MyD88 signaling pathway nitric oxide interleukin-6 interleukin-1β tumor necrosis factor a neuronal regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Up-regulation of mitochondrial antioxidation signals in ovarian cancer cells with aggressive biologic behavior

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第5期12卷 346-356页

作者： yue WANG Li DONG Heng CUI Dan-hua SHEN ying WANG Department of Obstetrics and Gynecology People's Hospital Peking University Beijing 100044 China Department of Obstetrics and Gynecology Beijing Jishuitan Hospital Peking University Beijing 100035 China Department of Pathology People's Hospital Peking University Beijing 100044 China

Objective： Recently, a high frequency of mutations in mitochondrial DNA （mtDNA） has been detected in ovarian cancer. To explore the alterations of proteins in mitochondria in ovarian cancer, a pair of human ovarian carcinoma cell lines （SKOV3/***1） with different metastatic potentials was examined. Methods： Cancer cells *** were derived from the ascitic tumor cells of nude mice bearing a tumor of ovarian cancer cells SKOV3. *** exhibited a higher degree of migration potential than its paired cell line SKOV3. The proteins in the mi- tochondria of these two cells were isolated and separated by 2-D gel electrophoresis. The differently expressed proteins were extracted and identified using matrix assisted laser desorption ionisation/time-of-flight/time-of-flight （MALDITOF/TOF）, and finally a selected protein candidate was further investigated by immunohistochemistry （IHC） method in nude mice bearing tumor tissues of these two cells. Results： A total of 35 spots with different expressions were identified between the two cells using 2D-polyacrylamide gel electrophoresis （PAGE） approach. Among them, 17 spots were detected only in either SKOV3 or *** cells. Eighteen spots expressed different levels, with as much as a three-fold difference between the two cells. Twenty spots were analyzed using MALDI-TOF/TOF, and 11 of them were identified successfully; four were known to be located in mitochondria, including superoxide dismutase 2 （SOD2）, fumarate hydratase （FH）, mitochondrial ribosomal protein L38 （MRPL38）, and mRNA turnover 4 homolog （MRTO4）. An increased staining of SOD2 was observed in *** over that of SKOV3 in IHC analysis. Conclusions： Our results indicate that the enhanced antioxidation and metabolic potentials of ovarian cancer cells might contribute to their aggressive and metastatic behaviors. The underlying mechanism warrants further study.

关键词： Ovarian carcinoma Mitochondria Invasion Proteomic Superoxide dismutase 2 (SOD2)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SmartPipe: Towards Interoperability of Industrial Applications via Computational Reflection

引用

Journal of Computer Science & Technology 2020年第1期35卷 161-178页

作者： Su Zhang hua-Qian Cai Yun Ma Tian-yue Fan ying Zhang Gang huang Key Laboratory of High-Confidence Software Technology(Peking University) Ministry of Education Beijing 100871China School of Software Tsinghua UniversityBeijing 100084China Hengyi Petrochemicals CO. LTD.Hangzhou 311215China National Engineering Research Center for Software Engineering Peking UniversityBeijing 100871China

With the advancement of new information technologies,a revolution is being taken place to bring the industry into a new era of intelligent *** of the key requirements of intelligent manufacturing is the interoperability of industrial ***,it is challenging to realize the interoperability for legacy industrial applications due to 1)the deficient semantic information of data transmitted over heterogeneous communication protocols,2)the difficulty to understand the complex process of business logic with no source code,and 3)the high cost and potential risk of reengineering the *** address the issues,in this paper,we propose an approach named SmartPipe to exposing existing functionalities of an industrial application as APIs without source code while simultaneously allowing the application to remain *** design a behavioral runtime model(BRM)as the self-representation of the industrial applications,based on which a computational reflection framework is designed to flexibly construct the model and generate APIs that encapsulate specific *** validate SmartPipe on a real industrial application that controls the spin-draw winding *** show that our approach is effective and more suitable for industrial scenes compared with traditional approaches.

关键词： computational reflection runtime model interoperability industrial application API generation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Weighted spectral correlation angle target detection method for land‑based hyperspectral imaging

引用

Frontiers of Optoelectronics 2023年第4期16卷 121-134页

作者： Qianghui Wang Bing Zhou Wenshen hua Jiaju ying Xun Liu yue Cheng Army Engineering University of PLA Shijiazhuang 050000China Army Engineering University of PLA Nanjing 210000China

In land-based spectral imaging,the spectra of ground objects are inevitably afected by the imaging conditions(weather conditions,atmospheric conditions,light conditions,zenith and azimuth angle conditions)and spatial distribution of targets,leading to uncertainties featured by“same object diferent spectrum”.That is,the spectrum of a ground object may change within a certain range under diferent imaging *** target detection(TD)methods are mainly based on similarity measurements and do not fully account for the spectral *** detection methods are prone to false detections or missed ***,reducing the impact of spectral uncertainties on TD is an important research topic in hyperspectral *** this paper,we frst review traditional TD methods and compare their principles and *** is found that the spectral correlation angle(SCA)method has good adaptability in land-based *** shortcoming of the SCA method that it cannot refect the local spectrum characteristics,is also *** the efect of spectral uncertainties cannot be completely overcome by the SCA method,a new similarity measurement method,the weighted spectral correlation angle(WSCA)method,is *** can reduce the infuence of spectral uncertainties on TD by increasing the weight of particular ***,we use two sets of experiments to analyze the efect of the WSCA method on *** performance in overcoming spectral uncertainties caused by variations in imaging conditions or uneven spatial distributions of targets is *** results show that the WSCA method can efectively reduce the infuence of spectral uncertainties and obtain a good detection result.

关键词： Hyperspectral image Spectral uncertain feature Target detection Land-based imaging condition Weighted spectral correlation angle(WSCA)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A fusion protein containing murine vascular endothelial growth factor and tissue factor induces thrombogenesis and suppression of tumor growth in a colon carcinoma model

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第8期9卷 602-609页

作者： Feng-ying huaNG yue-nan LI hua WANG Yong-hao huaNG ying-ying LIN Guang-hong TAN Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical College Haikou 571101 China

Induction of tumor vasculature occlusion by targeting a thrombogen to newly formed blood vessels in tumor tissues represents an intriguing approach to the eradication of primary solid tumors. In the current study, we construct and express a fusion protein containing vascular endothelial growth factor (VEGF) and tissue factor (TF) to explore whether this fusion protein has the capability of inhibiting tumor growth in a colon carcinoma model. The murine cDNA of VEGF A and TF were amplified by reverse transcriptase polymerase chain reaction (RT-PCR), and then cloned into prokaryotic expression plasmid pQE30 with a linker. The expression product recombinant VEGF-TF (rVEGF-TF) was purified and proved to have comparable enzyme activity to a commercial TF and the capability of specific binding to tumor vessels. Significant decrease of tumor growth was found in the mice administered with rVEGF-TF on Day 6 after initiated rVEGF-TF treatment (P<0.05), and the tumor masses in 2 of 10 mice were almost disappeared on Day 14 after the first treatment. In addition, valid thrombogenesis and tumor necrosis were observed in the tumor tissues injected with rVEGF-TF. Our results demonstrate that occlusion of tumor vasculature with rVEGF-TF is potentially an effective approach for cancer therapy.

关键词： Thrombogenesis Vascular endothelial growth factor (VEGF) Tissue factor (TF) Recombinant fusion protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：