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Advanced Pt hollow nanospheres/rubrene nanoleaves coupled with M-shaped DNA walker for ultrasensitive electrochemiluminescence bioassay

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Chinese Chemical Letters 2023年第6期34卷 247-251页

作者： Yumeng Song Xiuli Tao Wenbin Liang Xia Zhong Ruo Yuan ying zhuo Key Laboratory of Luminescence Analysis and Molecular Sensing Ministry of EducationCollege of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China

Herein,an intense electrochemiluminescence(ECL)was achieved based on Pt hollow nanospheres/rubrene nanoleaves(Pt HNSs/Rub NLs)without the addition of any coreactant,which was employed for ultrasensitive detection of carcinoembryonic antigen(CEA)coupled with an M-shaped DNA walker(M-DNA walker)as signal ***,in comparison with platinum nanoparticles(Pt NPs),Pt HNSs revealed excellent catalytic performance and pore confinement-enhanced ECL,which could significantly amplify ECL intensity of Rub NLs/dissolved O_(2)(DO)binary ***,the tracks and M-DNA walker were confined on the Pt HNSs simultaneously to promote the reaction efficiency,whose M-structure boosted the interaction sites between walking strands and tracks and reduced the rigidity of their *** the CEA approached the sensing interface,the M-DNA walker was activated based on highly specific aptamer recognition to recover ECL intensity with the assistance of exonucleaseⅢ(ExoⅢ).As proof of concept,the“on-off-on”switch aptasensor was constructed for CEA detection with a low detection limit of 0.20 fg/m *** principle of the constructed ECL aptasensor also enables a universal platform for sensitive detection of other tumor markers.

关键词： Platinum hollow nanospheres Electrochemiluminescence aptasensor M-shaped DNA walker On-off-on switch Carcinoembryonic antigen

CDs assembled metal-organic framework:Exogenous coreactant-free biosensing platform with pore confinement-enhanced electrochemiluminescence

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Chinese Chemical Letters 2022年第11期33卷 4803-4807页

作者： Xiu-Li Tao Mei-Chen Pan Xia Yang Ruo Yuan ying zhuo Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University) Ministry of EducationCollege of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China

Despite the various synthesis approachs to obtain luminous carbon dots(CDs),it is still quite challenging to construct the efficient electrochemiluminescence(ECL)owing to their low ECL reactivity and easy ***,an efficient and concise ECL system was skillfully constructed by taking advantage of the nitrogen and sulfur co-doped CDs(N,S-CDs)with surfaces rich in hydrazide groups as luminophors to emit intense ECL,and metal-organic framework(MOF)as the matrix to confine CDs in its ***,the proposed CDs assembled MOF(CDs/ZIF-8)enhanced anodic ECL signal up to 250%of pure CDs under the exogenous coreactant-free *** a proof of concept,the highly sensitive detection of uric acid(UA)was realized by the constructed ECL platform with a low detection limit of 3.52 nmol/L ranging from 10 nmol/L to 50μmol/*** work expanded ideas for the application of pore confinement effect,and provided references for the detection of disease biomarkers of gout and hyperuricemia.

关键词： Carbon dots Zeolitic imidazolate framework-8 Pore confinement-enhanced ECL Exogenous coreactant-free Uric acid

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冷轧油泥的无害化处理与资源化利用

现代化工 2024年第6期44卷 75-78页

作者：胡怡超金卓颖吴思王为初阙智明周晓龙华东理工大学化工学院上海200237

冷轧油泥是钢铁厂冷轧工艺产生的危险废弃物。作为油水固三相高度乳化的混合物,冷轧油泥组成十分复杂,若通过传统的焚烧或填埋法处理会造成严重的环境污染。目前处理冷轧油泥的工艺包括热解法、溶剂萃取法、热化学清洗法等,以将油固两... 详细信息

冷轧油泥是钢铁厂冷轧工艺产生的危险废弃物。作为油水固三相高度乳化的混合物,冷轧油泥组成十分复杂,若通过传统的焚烧或填埋法处理会造成严重的环境污染。目前处理冷轧油泥的工艺包括热解法、溶剂萃取法、热化学清洗法等,以将油固两相分离并分别回收。冷轧油泥废水是高浓度的有机废水,通常采用“预处理+深度降解”的复合工艺加以处理。通过油固两相的资源化,以及水相的减量化、无害化,可以实现危废品冷轧油泥的资源回收,变废为宝。

关键词：冷轧油泥热解溶剂萃取油脂回收水处理

Optimization on cationic liposome-mediated cell transfection of plasmid DNA

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The Chinese-German Journal of Clinical Oncology 2011年第5期10卷 290-292页

作者： Mingang ying Changhua zhuo Weidong Zang Department of Abdominal Surgery Fujian Provincial Cancer Hospital Teaching Hospital of Fujian Medical University Fuzhou 350014 China

Objective:The development of gene carriers for efficient gene delivery into cells has attracted growing attention in recent *** aim of this study was to achieve a better outcome of AAV-293 cells transfection by plasmid ***:We studied the optimal condition for higher efficiency of cationic lipid-mediated cell *** experimental groups were *** DNA and liposome were mixed in each groups at different ratios(μg:μL),1:2.5,1:3.5,1:4.0 and 1:5.0,*** gene functioned as reporter gene,measuring the transfection efficiency of the four groups using the method of X-gal ***:When the ratio was 1:3.5,the cell transfection rate was the *** the ratio of 1:2.5 recommended by product manual achieve the lowest transfection *** difference had statistical ***:In order to obtain a higher transfection efficiency,optimization on conditions of the ratio of plasmid DNA to liposome is necessary in cell transfection.

关键词： cell transfection cationic lipid plasmid DNA cell culture transfection efficiency

Selective hydrogenation of acetylene on graphene-supported non-noble metal single-atom catalysts

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Science China Materials 2020年第9期63卷 1741-1749页

作者： Hong-ying zhuo Xiaohu Yu Qi Yu Hai Xiao Xin Zhang Jun Li State Key Laboratory of Heavy Oil Processing College of Chemical EngineeringChina University of PetroleumBeijing 102249China Department of Chemistry and Key Laboratory of Organic Optoelectronics&Molecular Engineering of Ministry of Education Tsinghua UniversityBeijing 100084China Shaanxi Key Laboratory of Catalysis Shaanxi University of TechnologyHanzhong 723000China Department of Chemistry Southern University of Science and TechnologyShenzhen 518055China

Large-scale production of polyethylene in industry requires efficient elimination of the trace amount of acetylene ***,zeolite adsorption or the conversion of acetylene to ethylene via selective semi-hydrogenation on Pd catalysts is the commonly used *** this work,we investigate the reaction mechanisms of acetylene hydrogenation on defective graphene(DG)supported singleatom catalysts(SACs),M1/SV-G and M1/DV-G(M=Ni,Pd and Pt)using density functional theory(DFT),where SV-G and DV-G represent DG with single and double vacancies,*** is shown that the metal single-atoms(SAs)as well as their different coordination numbers both affect the activity and selectivity of the hydrogenation process.M1/DV-G provides better H2 dissociation ability than M1/SV-G,which accounts for the poor acetylene hydrogenation activity of M1/*** on the reaction barriers,Pt1/DV-G and Ni1/DV-G are better catalysts than other systems considered here,with Ni1/DV-G exhibiting high selectivity for the semi-hydrogenation product of *** results provide insights for the design of highly selective and noble-metal-free SACs for acetylene hydrogenation on carbon materials.

关键词： graphene single-atom catalysts acetylene hydrogenation density functional theory

Optical monitoring the degradation of PLGA inverse opal film

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Chinese Chemical Letters 2013年第1期24卷 9-12页

作者： Li-Guo Sun zhuo-ying Xie Yuan-Jin Zhao Hong-Mei Wei Zhong-Ze Gu State Key Laboratory of Bioelectronics Southeast University School of Chemistry and Materials Heilongjiang University

Implantable materials have broad applications in tissue engineering and in vivo sensors. It is essential to know the detailed information of the implantable materials during their degradation. In this paper, we developed a method to monitor the degradation process of a well-used biomaterial, poly（lactide-co- glycolide）（PLGA） by taking advantage of inverse opal structure. We found that mass loss, molecular weight and glass transition temperature of PLGA during the degradation process in Hank＇s artificial body fluid can be in situ monitored bv measuring the ootical orooerties of PLGA inverse ooal.

关键词： PLGA inverse opalDegradationOptical monitoring

Serum Thyroglobulin——A Sensitive Biomarker of Iodine Nutrition Status and Affected by Thyroid Abnormalities and Disease in Adult Populations

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Biomedical and Environmental Sciences 2017年第7期30卷 508-516页

作者： DU Yang GAO Yan Hui FENG zhuo ying MENG Fan Gang FAN Li Jun SUN Dian Jun Center for Endemic Disease Control Chinese Center for Disease Control and PreventionHarbin Medical UniversityKey Lab of Etiology and EpidemiologyNational Health and Family Planning Commission(23618504) Heilongjiang Land Reclamation Bureau General Hospital

Objective To evaluate the usefulness of the thyroglobulin （Tg） level in adults as a nutritiona biomarker of iodine status and to identify the factors related to the serum Tg level. Methods A cross-sectional study was conducted in adult populations of areas differing in iodine nutrition from three provinces （Autonomous Region） in China. Serum levels of thyroid hormones and Tg as well as thyroid autoantibodies were measured. The thyroid volume and nodule were measured by ultrasound. A multivariate linear regression analysis was used to assess iodine intake and other indeterminate factors associated with the serum Tg level. Results A total of 573 adults were recruited for this study. The serum Tg levels differed significantly among the three groups （22.27 ~g/L, 9.73 pg/L and 15.77 pg/L in the excess, more-than-adequate, and deficient groups, respectively）. The results of multivariate linear regression analysis indicate that excess and deficient iodine intake, goiter, thyroid nodule, hypothyroidism are significantly related with higher Tg level, and TgAb positivity is significantly related with lower serum Tg. Conclusion The serum Tg level reflects abnormal thyroid function and is a sensitive functiona biomarker of iodine nutrition status.

关键词： High iodine Iodine deficiency Thyroglobulin

Cloning and characterization of the gene cluster required for beauvericin biosynthesis in Fusarium proliferatum

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Science China(Life Sciences) 2013年第7期56卷 628-637页

作者： ZHANG Tao zhuo ying JIA XiaoPeng LIU JinTao GAO Hong SONG FuHang LIU Mei ZHANG LiXin CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences University of Chinese Academy of Sciences

Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. We isolated and characterized the fpBeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus Fusarium proliferatum LF061. Targeted inactivation of the F. proliferatum genomic copy of fpBeas abolished the production of beauvericin. Comparative sequence analysis of the FpBEAS showed 74% similarity with the BbBEAS that synthesizes the cyclic trimeric ester beauvericin in Beauveria bassiana, which assembles N-methyl-dipeptidol monomer intermediates by the programmed iterative use of the nonribosomal peptide synthetase modules. Differences between the organization of the beauvericin loci in F. proliferaturm and B. bassiana revealed the mechanism for high production of beauvericin in F. proliferatum. Our work provides new insights into beauvericin biosynthesis, and may lead to beauvericin overproduction and creation of new analogs via synthetic biology approaches.

关键词： beauvericin Fusarium proliferatum biosynthesis gene cluster synthetic biology

Triazine COF-supported single-atom catalyst (Pd/trzn-COF) for CO oxidation

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Science China Materials 2021年第8期64卷 1939-1951页

作者： Yin-Juan Chen Hong-ying zhuo Yuan Pan Jin-Xia Liang Chen-Guang Liu Jun Li State Key Laboratory of Heavy Oil Processing College of Chemical EngineeringChina University of Petroleum(East China)Qingdao 266580China Department of Chemistry Key Laboratory of Organic Optoelectronics&Molecular Engineering of Ministry of EducationTsinghua UniversityBeijing 100084China Department of Chemistry Southern University of Science and TechnologyShenzhen 518055China

Single-atom catalysts(SACs) with well-defined and specific single-atom dispersion on supports offer great potential for achieving both high catalytic activity and selectivity. Covalent organic frameworks(COFs) with tailormade crystalline structures and designable atomic composition is a class of promising supports for SACs. Herein, we have studied the binding sites and stability of Pd single atoms(SAs)dispersed on triazine COF(Pd1/trzn-COF) and the reaction mechanism of CO oxidation using the density functional theory(DFT). By evaluating different adsorption sites, including the nucleophilic sp2C atoms, heteroatoms and the conjugated π-electrons of aromatic ring and triazine, it is found that Pd SAs can stably combine with trzn-COF with a binding energy around-5.0 eV, and there are two co-existing dynamic Pd1/trzn-COFs due to the adjacent binding sites on trzn-COF. The reaction activities of CO oxidation on Pd1/trzn-COF can be regulated by the anion–π interaction between a +δ phenyl center and the related-δ moieties as well as the electron-withdrawing feature of imine in the specific complexes. The Pd1/trzn-COF catalyst is found to have a high catalytic activity for CO oxidation via a plausible tri-molecular Eley-Rideal(TER) reaction mechanism. This work provides insights into the d–π interaction between Pd SAs and trznCOF, and helps to better understand and design new SACs supported on COF nanomaterials.

关键词： single-atom catalysts Pd loading covalent organic frameworks CO oxidation