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镉离子诱导BA/F3β细胞发生奇特的细胞凋亡

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生物化学与生物物理学报 2000年第1期32卷 77-80页

作者：翟琦巍季红斌郑仲承孙兰英刘新垣中国科学院上海生物化学研究所!上海200031

细胞凋亡一般都伴随有ＤＮＡ片段化，活性氧含量增加，并能被过量的Ｂｃｌ２所抑制。以ＢＡ／Ｆ３β细胞为模型，利用ＭＴＴ检测、Ｈｏｃｈｅｓｔ染色以及透射电镜检测等技术却发现，镉离子虽然可以诱导该细胞凋亡，但是这... 详细信息

细胞凋亡一般都伴随有ＤＮＡ片段化，活性氧含量增加，并能被过量的Ｂｃｌ２所抑制。以ＢＡ／Ｆ３β细胞为模型，利用ＭＴＴ检测、Ｈｏｃｈｅｓｔ染色以及透射电镜检测等技术却发现，镉离子虽然可以诱导该细胞凋亡，但是这种凋亡没有ＤＮＡ片段化，也没有活性氧含量增加。此外，过量Ｂｃｌ２对这种凋亡也没有保护作用。因此，可以确认镉离子诱导ＢＡ／Ｆ３β细胞发生了奇特的细胞凋亡。

关键词：细胞凋亡 DNA片段化活性氧 Bcl2 镉离子

Early identification of stroke through deep learning with multi-modal human speech and movement data

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Neural Regeneration Research 2025年第1期20卷 234-241页

作者： Zijun Ou Haitao Wang Bin Zhang Haobang Liang Bei Hu Longlong Ren Yanjuan liu Yuhu Zhang Chengbo Dai Hejun Wu Weifeng Li xin Li School of Computer Science and Engineering Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Department of Neurology Guangdong Neuroscience InstituteGuangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Emergency Medicine Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong ProvinceChina

Early identification and treatment of stroke can greatly improve patient outcomes and quality of *** clinical tests such as the Cincinnati Pre-hospital Stroke Scale(CPSS)and the Face Arm Speech Test(FAST)are commonly used for stroke screening,accurate administration is dependent on specialized *** this study,we proposed a novel multimodal deep learning approach,based on the FAST,for assessing suspected stroke patients exhibiting symptoms such as limb weakness,facial paresis,and speech disorders in acute *** collected a dataset comprising videos and audio recordings of emergency room patients performing designated limb movements,facial expressions,and speech tests based on the *** compared the constructed deep learning model,which was designed to process multi-modal datasets,with six prior models that achieved good action classification performance,including the I3D,SlowFast,X3D,TPN,TimeSformer,and *** found that the findings of our deep learning model had a higher clinical value compared with the other ***,the multi-modal model outperformed its single-module variants,highlighting the benefit of utilizing multiple types of patient data,such as action videos and speech *** results indicate that a multi-modal deep learning model combined with the FAST could greatly improve the accuracy and sensitivity of early stroke identification of stroke,thus providing a practical and powerful tool for assessing stroke patients in an emergency clinical setting.

关键词： artificial intelligence deep learning diagnosis early detection FAST screening stroke

Role of the globus pallidus in motor and non-motor symptoms of Parkinson's disease

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Neural Regeneration Research 2025年第6期20卷 1628-1643页

作者： Yimiao Jiang Zengxin Qi Huixian Zhu Kangli Shen Ruiqi liu Chenxin Fang Weiwei Lou Yifan Jiang Wangrui Yuan xin Cao Liang Chen Qianxing Zhuang Department of Physiology School of MedicineNantong UniversityNantongJiangsu ProvinceChina Department of Neurosurgery Huashan HospitalShanghai Medical CollegeFudan UniversityShanghaiChina National Center for Neurological Disorders ShanghaiChina Shanghai Key Laboratory of Brain Function Restoration and Neural Regeneration ShanghaiChina State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institute of Brain ScienceFudan UniversityShanghaiChina

The globus pallidus plays a pivotal role in the basal ganglia circuit. Parkinson's disease is characterized by degeneration of dopamine-producing cells in the substantia nigra, which leads to dopamine deficiency in the brain that subsequently manifests as various motor and non-motor symptoms. This review aims to summarize the involvement of the globus pallidus in both motor and non-motor manifestations of Parkinson's disease. The firing activities of parvalbumin neurons in the medial globus pallidus, including both the firing rate and pattern, exhibit strong correlations with the bradykinesia and rigidity associated with Parkinson's disease. Increased beta oscillations, which are highly correlated with bradykinesia and rigidity, are regulated by the lateral globus pallidus. Furthermore,bradykinesia and rigidity are strongly linked to the loss of dopaminergic projections within the cortical-basal ganglia-thalamocortical loop. Resting tremors are attributed to the transmission of pathological signals from the basal ganglia through the motor cortex to the cerebellum-ventral intermediate nucleus circuit. The cortico–striato–pallidal loop is responsible for mediating pallidi-associated sleep disorders. Medication and deep brain stimulation are the primary therapeutic strategies addressing the globus pallidus in Parkinson's disease. Medication is the primary treatment for motor symptoms in the early stages of Parkinson's disease, while deep brain stimulation has been clinically proven to be effective in alleviating symptoms in patients with advanced Parkinson's disease,particularly for the movement disorders caused by levodopa. Deep brain stimulation targeting the globus pallidus internus can improve motor function in patients with tremordominant and non-tremor-dominant Parkinson's disease, while deep brain stimulation targeting the globus pallidus externus can alter the temporal pattern of neural activity throughout the basal ganglia–thalamus network. Therefore, the comp

关键词： anxiety basal ganglia bradykinesia deep brain stimulation depression globus pallidus externus globus pallidus internus lateral globus pallidus medial globus pallidus neural circuit Parkinson's disease

颌骨骨髓基质细胞与牙周膜细胞的生物学特性比较

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牙体牙髓牙周病学杂志 2012年第2期22卷 66-70页

作者：王欣房明刘敏杰董广英王新文刘玲侠王勤涛第四军医大学口腔医学院陕西西安710032

目的:比较颌骨骨髓基质细胞与牙周膜细胞的基本生物学特性。方法:分离颌骨来源骨髓基质细胞和牙周膜细胞,进行改良体外原代培养。倒置显微镜观察细胞生长及克隆形成情况;CCK-8检测细胞生长曲线;免疫荧光染色检测STR0-1表达;成脂诱导后... 详细信息

目的:比较颌骨骨髓基质细胞与牙周膜细胞的基本生物学特性。方法:分离颌骨来源骨髓基质细胞和牙周膜细胞,进行改良体外原代培养。倒置显微镜观察细胞生长及克隆形成情况;CCK-8检测细胞生长曲线;免疫荧光染色检测STR0-1表达;成脂诱导后检测脂滴形成,矿化诱导后检测碱性磷酸酶的变化并用RT-PCR检测7、14 d成骨相关基因OCN的表达水平。结果:两种细胞体外培养均呈成纤维样细胞外形,能克隆生长,均具有活跃的增殖能力;两种细胞STR0-1表达阳性;成脂诱导后可见脂滴形成;矿化诱导后骨髓基质细胞的碱性磷酸酶活性较强,而且OCN基因表达较早且较强。结论:颌骨来源骨髓基质细胞具有较强的增殖及成骨分化能力,具有干细胞特性,可能是有较大临床应用潜力的组织工程种子细胞。

关键词：颌骨人骨髓基质细胞人牙周膜细胞潜能

酪氨酸激酶Jak3与核小体组装蛋白Nap1的相互作用

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生物化学与生物物理学报 2000年第1期32卷 4-8页

作者：季红斌翟琦巍朱锦芳孙兰英刘新垣郑仲承中国科学院上海生物化学研究所!上海200031

在白细胞介素２（ｉｎｔｅｒｌｅｕｋｉｎ２，ＩＬ２）的信号转导过程中，酪氨酸激酶Ｊａｋ３起着至关重要的作用。它不但参与ＪａｋＳｔａｔ通路的传递，而且通过与一些未知的信号分子相互作用调节一些原癌基因的表达， ... 详细信息

在白细胞介素２（ｉｎｔｅｒｌｅｕｋｉｎ２，ＩＬ２）的信号转导过程中，酪氨酸激酶Ｊａｋ３起着至关重要的作用。它不但参与ＪａｋＳｔａｔ通路的传递，而且通过与一些未知的信号分子相互作用调节一些原癌基因的表达，如ｃｆｏｓ，ｃｍｙｃ等。由于目前越来越多的证据表明在信号转导过程中两个蛋白质之间的相互作用需要磷酸酪氨酸的存在，为此构建了酪氨酸磷酸化相关的酵母双杂交系统，以进一步阐释Ｊａｋ３在ＩＬ２信号通路中的作用。利用这个系统，以Ｊａｋ３的Ｎ端ＪＨ３～ＪＨ７区域筛选外周血白细胞ｃＤＮＡ库。结果得到了５０多个双阳性克隆。测序结果表明其中一个为核小体组装蛋白１（Ｎａｐ１）的基因，它编码３９２个氨基酸残基。实验还证明Ｎａｐ１与Ｊａｋ３之间的相互作用在一定程度上依赖于酪氨酸磷酸化。用免疫共沉淀及蛋白质印迹实验证明在小鼠原Ｂ淋巴细胞株ＢＡＦ／ＢＯ３β细胞中确实存在着核小体组装蛋白Ｎａｐ１与酪氨酸激酶Ｊａｋ３Ｎ端的相互作用。

关键词： Jak3 信号转导酪氨酸磷酸化相关的酵母双杂交系统核小体组装蛋白1(Nap1)

泛蛋白连接酶Ubc9参与氧还蛋白Ref 1的降解

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生物化学与生物物理学报 2000年第1期32卷 63-68页

作者：严明达徐蔚晶鲁林荣孙兰英刘新垣郑仲承中国科学院上海生物化学研究所!上海200031

氧还蛋白Ｒｅｆ１是一种双功能蛋白质，在细胞氧还调控和ＤＮＡ无嘌呤／无嘧啶损伤修复中起重要作用。为寻找与它相互作用的蛋白Ｒｉｐｓ（Ｒｅｆ１ｉｎｔｅｒａｃｔｉｎｇｐｒｏｔｅｉｎｓ），用Ｒｅｆ１氧还功能域进行... 详细信息

氧还蛋白Ｒｅｆ１是一种双功能蛋白质，在细胞氧还调控和ＤＮＡ无嘌呤／无嘧啶损伤修复中起重要作用。为寻找与它相互作用的蛋白Ｒｉｐｓ（Ｒｅｆ１ｉｎｔｅｒａｃｔｉｎｇｐｒｏｔｅｉｎｓ），用Ｒｅｆ１氧还功能域进行了酵母双杂交库的筛选，得到了５种阳性克隆。其中Ｒｉｐ３经测序证实为泛蛋白连接酶Ｕｂｃ９。Ｈｅｌａ细胞中共过表达Ｕｂｃ９可以明显抑制Ｒｅｆ１对ＡＰ１报告系统的增强作用。Ｗｅｓｔｅｒｎ印迹发现Ｒｅｆ１的蛋白质水平由于Ｕｂｃ９的共过表达而下调。这些结果表明Ｕｂｃ９参与了Ｒｅｆ１的蛋白质降解，从而下调了Ｒｅｆ１的生理功能。

关键词： Ref1 Ubc9 泛蛋白化降解酵母双杂交系统

Plasmon-induced local electric field improved hydrogen evolution reaction on Ag/Mo_(2)C nanosheets

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Nano Research 2025年第1期18卷 270-277页

作者： HuangJingWei Li xin Zi Jun Wu xinli Wang Li Zhu Qiyou Wang Jianfu Han Junwei Fu Hongmei Li Kai Huang Yu Chen Min liu School of Physics State Key Laboratory of Powder MetallurgyHunan Joint International Research Center for Carbon Dioxide Resource UtilizationCentral South UniversityChangsha 410083China School of Metallurgy and Environment Central South UniversityChangsha 410083China Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Nano-Institute Munich Faculty of PhysicsLudwig-Maxilimians-Universität MünchenMünchen 80539Germany Hunan Institute of Advanced Sensing and Information Technology Xiangtan UniversityXiangtan 411105China

Electrocatalytic water splitting offers a promising way for hydrogen production with near-zero ***,such as molybdenum carbides(Mo_(2)C),are promising materials for hydrogen evolution reaction(HER)but still suffer from poor intrinsic water activation ***,we developed a plasmon-induced local electric field(PILEF)strategy to solve this ***(Ag)nanoparticles decorated Mo_(2)C nanosheets(Ag/Mo_(2)C)were successfully prepared by electrostatic *** visible light excited the PILEF on Ag/Mo_(2)C remarkably reducing the activation energy by 92.7 kJ·mol^(-1)from 147.3 kJ·mol^(-1)of Mo_(2)C to 54.6 kJ·mol^(-1).As a result,the plasmonic Ag/Mo_(2)C significantly enhances~2.3-fold of the current density from 2.8 mA·cm^(-2)of Mo_(2)C to 6.5 mA·cm^(-2)at-3 V *** and reduces the overpotential by 104 mV from 403 mV of dark state to 299 mV of light state at the current density of 10 mA·cm^(-2),achieving better performance than reported *** research demonstrates that PILEF enhances HER activities,offering a potential strategy for boosting the intrinsic activities of catalysts.

关键词： sliver particles plasmon effect field effect hydrogen evolution reaction(HER) electrocatalysis

Contamination characteristics of heavy metals in the water of Daqing Wetlands

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Journal of Shanghai University(English Edition) 2010年第4期14卷 262-265页

作者：张新颖刘晓艳王君王珍珍苏鹏程钟成林曹正楠 School of Environmental and Chemical Engineering Shanghai University

Daqing Wetland was contaminated by heavy metals for holding lots of industrial and domestic waste water. Due to their high stability and non-degradation, heavy metals are possible to gather in human body through the food chain and then threaten health. Water samples were collected from Longfeng Wetland and lake marshes in the vicinity of Daqing City. The contents of heavy metals （Cd, Pb, Cr, Cu, Zn, Ni） were determined, and the contamination degrees were evaluated simultaneously. The results indicate that heavy metals of Zn, Ni, Cd, Cr and Pd have contaminated the Longfeng Wetland severely. In addition the concentrations of Cr and Cd in some lakes around oil wells are excessively high, and have overrun the grade V based on the threshold values of surface water given by the National Environmental Protection Agency of China. Some measures have to be taken to tackle heavy metal contamination of Longfeng Wetland and lake marshes in Daqing City.

关键词： Daqing City wetland heavy metal contamination evaluation

Gut-brain axis as a bridge in obesity and depression:Mechanistic exploration and therapeutic prospects

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World Journal of Psychiatry 2025年第1期15卷 12-20页

作者： Rui-ying Fang Xiao-Rui Pan xin-xing Zeng Zheng-Zheng Li Bo-Fan Chen Hai-Min Zeng Jie Peng The Second Clinical Medical College The Second Affiliated HospitalJiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina

A recent study by Wang et al,published in the World Journal of Psychiatry,provided preventative and therapeutic strategies for the comorbidity of obesity and *** gut-brain axis,which acts as a two-way communication system between the gastrointestinal tract and the central nervous system,plays a pivotal role in the pathogenesis of these *** suggests that metabolic byproducts,such as short-chain fatty acids,lipopolysaccharide and bile acids,which are generated by the gut microbiota,along with neurotransmitters and inflammatory mediators within the gut-brain axis,modulate the host's metabolic processes,neuronal regulation,and immune responses through diverse *** interaction between obesity and depression via the gut-brain axis involves disruptions in the gut microbiota balance,inflammatory immune responses,and alterations in the neuroendocrine *** the gut-brain axis,for example,through a ketogenic diet,the use of probiotics,and the supplementation of antioxidants,offers new remedial approaches for obesity and *** research that explores the mechanisms of the gut-brain axis is needed to provide more evidence for clinical treatment.

关键词： Gut-brain axis Obesity Depression Gut microbiota Mechanism