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Pedosphere 2005年第4期15卷 448-455页

作者： LIU ying HAN Shi-jie ZHOU Yu-Mei ZHANG Jun-Hui Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 （China）.

Total and root-severed soil respiration rates for five plots set up 50 m apart in a Betula ermanii Cham.-dark coniferous forest ecotone on a north-facing slope of the Changbai Mountains, China, were measured to evaluate the seasonal variations of soil respiration, to assess the effect of soil temperature and water content on soil respiration, and to estimate the relative contributions of root respiration to the total soil respiration. PVC cylinders in each of 5 forest types of a B. ermanii-dark coniferous forest ecotone were used to measure soil respirations both inside and outside of the cylinders. The contribution of roots to the total soil respiration rates ranged from 12.5% to 54.6%. The mean contribution of roots for the different plots varied with the season, increasing from 32.5% on June 26 to 36.6% on August 3 and to 41.8% on October 14. In addition, there existed a significant (P < 0.01) logarithmic relationship between total soil respiration rate and soil temperature at 5 cm soil depth. Also, a similar trend was observed for the soil respiration and soil water content at the surface (0-5 cm) during the same period of time.

关键词： Betula ermanii Cham. dark coniferous forest ecotone root respiration soil respiration

The expression of Lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2

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Journal of Integrative Agriculture 2020年第12期19卷 3054-3064页

作者： ZHANG Ya-ni HU Cai WANG ying-jie ZUO Qi-sheng LI Bi-chun Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education Yangzhou UniversityYangzhou 225009P.R.China

Lin28A and Lin28B are homologous RNA-binding proteins that participate in the development of primordial germ cells. The mechanisms underlying expression and regulation of Lin28A have been well documented, but such information for Lin28B is limited. In this study, a fragment of the Lin28B promoter was cloned, the pEGFP-pLin28B vector was constructed. DF-1 chicken fibroblasts were transfected and the expression of green fluorescent protein (GFP) was measured. Furtherly, Lin28B promoter of different lengths fragments was cloned using the chromosome-walking method and the fragments were ligated into the PGL3-Basic vector, and transfected into DF-1 cells. Results of dual-luciferase reporter assay showed that the core of the Lin28B promoter was included in the sequence from –1 431 to –1 034 bp. The binding sites of the transcription factor TCF7L2 was showed within this sequence by bioinformatics analysis. The promoter activity of Lin28B was downregulated (Pying Lin28B expression.

关键词： primordial germ cells Lin28B promoter H3K4me2 Wnt5a/β-catenin/TCF7L2

Weichang'an and 5-fluorouracil suppresses colorectal cancer in a mouse model

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World Journal of Gastroenterology 2015年第4期21卷 1125-1139页

作者： Li Tao Jin-Kun Yang ying Gu Xin Zhou Ai-Guang Zhao Jian Zheng ying-jie Zhu Department of Oncology Longhua Hospital Shanghai University of Traditional Chinese Medicine Department of Pharmacy Longhua HospitalShanghai University of Traditional Chinese Medicine

AIM: To examine the effect of Weichang'an(WCA) and 5-fluorouracil(5-Fu) on colorectal tumor and hepatic metastasis in a mouse model. METHODS: Quantitative determination of hesperidin, the effective component in WCA decoction, was performed using Hp LC. In vitro cytotoxicity of WCA was determined by treating HCT-116 cells with WCA diluents or serum extracted from rats that received WCA by oral gavage for 1 wk and MTT assays. Forty-eight nude mice received cecum implantation with tumor blocks subcutaneously amplified from human colon cancer cell line HCT-116. Mice were randomly divided into four treatment groups: control(CON), WCA, 5-Fu and combination(WCA + 5-Fu). pathological examination of tumors consisted of tissue sectioning and hematoxylin and eosin staining. Tumor weight and size were measured, and the number of metastatic l e s i o n s wa s c o u n t e d. S e r u m c a r c i n o e m b r yo n i c antigen(CEA) level was determined by ELISA. The expression levels of tumor genesis and metastasisrelated proteins β-catenin and matrix metalloproteinase(MMp)-7 were measured by real-time quantitative reverse transcriptase polymerase chain reaction(RTp CR), immunohistochemistry and immunoblotting. Cell fractionation was used to investigate intracellular distribution of β-***: parenchymal tumors were palpable in the abdomens of nude mice 2 wk post-implantation and orthotopic tumor formation rate was 100% in all groups. 5-Fu treatment alone significantly decreased tumor weight compared to the CON group(1.203 ± 0.284 g vs 1.804 ± 0.649 g, p < 0.01). WCA treatment alone reduced the rate of metastasis(50% vs 100%,p < 0.05). Combination treatment of WCA + 5-Fu was the most effective, reducing the tumor weight(1.140 ± 0.464 g vs 1.804 ± 0.649 g, p < 0.01) and size(1493.438 ± 740.906 mm3 vs 2180.259 ± 816.556 mm3, p < 0.05), the rate of metastases(40% vs 100%, p < 0.01), and serum CEA levels(31.263 ± 7.421 μg/L vs 43.040 ± 11.273 μg/L, p < 0.05). Expression of β-cat

关键词： Colorectal cancer Hepatic metastasis Weichang’an f

A COMPARISON OF THE RETRIEVAL OF ATMOSPHERIC TEMPERATURE PROFILES USING OBSERVATIONS OF THE 60 GHZ AND 118.75 GHZ ABSORPTION LINES

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Journal of Tropical Meteorology 2018年第2期24卷 151-162页

作者： HE Qiu-rui WANG Zhen-zhan HE jie-ying ZHANG Lan-jie Key Laboratory of Microwave Remote Sensing National Space Science Center Chinese Academy of SciencesBeijing 100190 China University of Chinese Academy of Sciences Beijing 100049 China

The Microwave Temperature Sounder-Ⅱ(MWTS-Ⅱ) and Microwave Humidity and Temperature Sounder(MWHTS) onboard the Fengyun-3 C(FY-3 C) satellite can be used to detect atmospheric temperature profiles. The MWTS-II has 13 temperature sounding channels around the 60 GHz oxygen absorption band and the MWHTS has 8 temperature sounding channels around the 118.75 GHz oxygen absorption line. The data quality of the observed brightness temperatures can be evaluated using atmospheric temperature retrievals from the MWTS-Ⅱ and MWHTS observations. Here, the bias characteristics and corrections of the observed brightness temperatures are described. The information contents of observations are calculated, and the retrieved atmospheric temperature profiles are compared using a neural network(NN) retrieval algorithm and a one-dimensional variational inversion(1 D-var) retrieval algorithm. The retrieval results from the NN algorithm show that the accuracy of the MWTS-Ⅱ retrieval is higher than that of the MWHTS retrieval, which is consistent with the results of the radiometric information analysis. The retrieval results from the 1 D-var algorithm show that the accuracy of MWTS-Ⅱ retrieval is similar to that of the MWHTS retrieval at the levels from 850-1,000 h Pa, is lower than that of the MWHTS retrieval at the levels from 650-850 h Pa and 125-300 h Pa, and is higher than that of MWHTS at the other levels. A comparison of the retrieved atmospheric temperature using these satellite observations provides a reference value for assessing the accuracy of atmospheric temperature detection at the 60 GHz oxygen band and 118.75 GHz oxygen line. In addition, based on the comparison of the retrieval results, an optimized combination method is proposed using a branch and bound algorithm for the NN retrieval algorithm, which combines the observations from both the MWTS-Ⅱand MWHTS instruments to retrieve the atmospheric temperature profiles. The results show that the optimal combination can further i

关键词： Fengyun-3C satellite Microwave Temperature Sounder-II microwave humidity and temperature sounder one-dimensional variational retrieval algorithm neural networks retrieval algorithm

Identification of a novel gain-of-function mutant allele,slr1-d5,of rice DELLA protein

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Journal of Integrative Agriculture 2016年第7期15卷 1441-1448页

作者： ZHANG Yun-hui BIAN Xiao-feng ZHANG Suo-bing LING Jing WANG ying-jie WEI Xiao-ying FANG Xian-wen The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm/Jiangsu Provincial Key Laboratory of Agrobiology/Institute of Food Crops Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Shandong Business Institute Yantai 264670P.R.China

Controling the height of crops plays a crucial role for their yields. The large scale utilization of semi-dwarf varieties has greatly improved crop yield, providing an effective support for world food security. In rice, a main food for over half of the world’s population, a number of dwarf loci have been identiifed. However, most of them are recessive, such as the ‘green revolution’ genesd1. To gain more beneifcial loci for rice breeding programs, exploring new mutations is needed, especialy the dominant loci which can be used broadly for hybrid breeding. Here, we isolated a novel dominant dwarf rice mutant, slr1-d5. Al of the internodes ofslr1-d5are reduced. We ifnd that the responsiveness ofslr1-d5to gibberelin （GA）, GA3, was signiifcantly reduced. Map-based cloning revealed that the dominant dwarifsm ofslr1-d5was caused by an amino acid substitution in the N-terminal TVHYNP domain of rice DELLA protein, SLR1, where the conserved amino acid Pro （P） was substituted to His （H）. Our ifndings not only further prove the pivotal role of TVHYNP motif in regulating SLR1 stability, but also provide a new dwarf source for improvement of rice germplasms.

关键词： rice dominant dwarf DELLA protein gibberelin

Engineering intermolecular charge transfer in chiral organometallic crystals by rational doping for high-contrast CPL switches

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Science China Chemistry 2023年第7期66卷 2011-2018页

作者： ying-jie Liu Liang Chen Zhao-Yang Wang Xi-Yan Dong Ya-Ke Li Shuang-Quan Zang Henan Key Laboratory of Crystalline Molecular Functional Materials Henan International Joint Laboratory of Tumor Theranostical Cluster MaterialsGreen Catalysis Center and College of ChemistryZhengzhou UniversityZhengzhou 450001China College of Chemistry and Chemical Engineering Henan Polytechnic UniversityJiaozuo 454003China

The development of circularly polarized luminescence(CPL)switches is of great importance but ***,a charge donor/acceptor pair comprising two chiral gold(I)-isocyanide complexes was designed to construct light-harvesting system via intermolecular charge-transfer(CT)*** doping nonemissive S-Au I into blue-emitting S-AuCN,(S-Au CN)_(1-x)(S-AuI)_(x)(0≤x≤3.4%)with tunable emission from blue to red was *** large red-shifted emission was realized based on the remarkable change of the electronic properties between the S-Au CN dimer and red-emitting(S-A CN)-(S-AuI)CT pair,and the resulting energy-transfer(En T)process between ***,the En T process can be switched off/on by external stimuli of grinding and CH_(2)Cl_(2)fuming,giving rise to high-contrast(blue versus red)CPL switching *** study opens a novel avenue for developing CPL switches by constructing light-harvesting CT-doped systems based on chiral organometallic complexes.

关键词： charge transfer energy transfer crystal engineering circularly polarized luminescence gold complex

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Tungsten control in type-I ELMy H-mode plasmas on EAST

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Nuclear Science and Techniques 2021年第9期32卷 61-72页

作者： Zong Xu Zhen-Wei Wu Ling Zhang Yun-Xing Cheng Wei Gao Xiao-Dong Lin Xiang Gao ying-jie Chen Yue-Heng Huang Juan Huang Qing Zang Yin-Xian jie Yan-Min Duan ying-ying Li Hai-Qing Liu Guo-Sheng Xu EAST team Advanced Energy Research Center Shenzhen UniversityShenzhen 518060China Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China Institute of Plasma Physics Chinese Academy of ScienceHefei 230031China University of Science&Technology of China Hefei 230031China

The first experimental investigation of the tungsten behavior in ELMy H-mode plasmas with co-/counter neutral beam injection(NBI)and unfavor-able/favorable B t was performed on *** was found to accumulate easily in ELMy H-mode plasma with co-NBI heating and unfavorable B ***,in this case the tungsten concentration can exceed 10^(-4),resulting in degradation of the plasma confinement and periodic H–L *** reduce the tungsten concentration in steady-state type-I ELMy H-mode operation,counter-NBI is applied to modify the density and temperature and brake the plasma toroidal *** applied counter-NBI decreases the PHZ+E_(r) inward pinch velocity and rever-ses the direction of neoclassical inward convection,thus decreasing the tungsten concentration from-7×10^(-5) to-2×10^(-5) in type-I ELMy H-mode plasma with favorable B_(t).A comparison of the effects of different B_(t) directions on the tungsten behavior also shows that favor-able B_(t) is beneficial for reducing the tungsten concentration in the core *** results imply that counter-NBI with favorable B_(t) can effectively prevent tungsten accu-mulation and expand the operating window for exploring steady-state type-I ELMy H-mode operation of EAST.

关键词： Tungsten accumulation Counter-NBI Favorable B_(t)ELMy H-mode EAST

EXPERIMENTAL RESEARCH ON THE SHAPE CHARACTERS OF NATURAL AND VENTILATED SUPERCAVITATION

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Journal of Hydrodynamics 2007年第5期19卷 564-571页

作者： ZHANG Xue-wei WEI ying-jie ZHANG Jia-zhong WANG Cong YU Kai-ping School of Astronautics Harbin Institute of Technology Harbin 150001 China

A series of projectile and water-tunnel experiments were conducted to investigate the shape characters of natural and ventilated supercavitation. It was found that the shape and dimensions of both the natural supercavitation and the ventilated supercavitation are similar through the comparison of them when the cavitation number is small and equal, and the contour of both the natural supercavitation and the ventilated supercavitation can be calculated effectively with the Savchenko formula. The gravity effect can induce the asymmetry of ventilated supercavitation, and the asymmetry is more apparent as the Froude number is smaller. The empirical formula for calculating the axial deformation of ventilated supercavitation was corrected and expanded based on experiment data. The evolution rules of both the natural supercavitation and the ventilated supercavitation were described, and the vortex frequency of natural cavitation was obtained. In addition, the hysteretic aspect was observed between the development and the fall process of ventilated cavitation.

关键词： natural cavitation ventilated cavitation shapecharacters water tunnel experiment projectile experiment

Cavity formation during water entry of heated spheres

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Chinese Physics B 2018年第9期27卷 380-388页

作者： Jia-Chuan Li ying-jie Wei＊ Cong Wang and Wei-Xue Xia School of Astronautics Harbin Institute of TechnologyHarbin 150001China

We experimentally study the cavity formation when heated spheres impact onto water at low and high *** observations present that the formation and appearance of the cavity are affected by the boiling modes and the heat transfer intensity. In the nucleate-boiling regime, a rough cavity can be formed at a rather low impact velocity, while at the same velocity, the cavity formed in the film-boiling regime may have a very smooth interface with a stable vapor layer around the sphere. We discuss the effects of the impact speed, water and sphere temperatures on the stability of the vapor layer. For low subcooled water, the stable vapor layer will be disturbed when increasing the impact velocity, leading to a disturbed cavity. For high subcooled water, the film boiling has a particular boiling model in which the vapor layer around the sphere cannot keep its stability. In this particular film-boiling regime, no cavities can be formed at low impact velocities and only broken cavities can be formed at high impact velocities.

关键词： water-entry cavity boiling vapor layer

Shale gas production evaluation framework based on data-driven models

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Petroleum Science 2023年第3期20卷 1659-1675页

作者： You-Wei He Zhi-Yue He Yong Tang ying-jie Xu Ji-Chang Long Kamy Sepehrnoori State Key Laboratoryofiland Gas Rseroir Geoogy and Exloitation Southwest Petroleum UniverstyChengdu00SichuanChina Department of Petroleum and Geosystems Engineering The University of Texas at AustinAustinTX78712UA

Increasing the production and utilization of shale gas is of great significance for building a clean and low-carbon energy *** decline of gas production has been widely observed in shale gas *** to forecast shale gas production is still challenging due to complex fracture networks,dynamic fracture properties,frac hits,complicated multiphase flow,and multi-scale flow as well as data quality and *** work develops an integrated framework for evaluating shale gas well production based on data-driven ***,a comprehensive dominated-factor system has been established,including geological,drilling,fracturing,and production *** processing and visualization are required to ensure data quality and determine final data set.A shale gas production evaluation model is developed to evaluate shale gas production ***,the random forest algorithm is used to forecast shale gas *** prediction accuracy of shale gas production level is higher than 95%based on the shale gas reservoirs in ***-one wells are randomly selected to predict cumulative gas production using the optimal regression *** proposed shale gas production evaluation frame-work overcomes too many assumptions of analytical or semi-analytical models and avoids huge computation cost and poor generalization for numerical modelling.

关键词： Shale gas Production evaluation Production prediction Data-driven models Carbon neutrality