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Influence of the aspect ratio on the endovascular treatment of intracranial aneurysms: A computational investigation

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Journal of Biomedical Science and Engineering 2012年第8期5卷 422-431页

作者： Abraham Yik-Sau Tang Siu-Kai Lai Kar-Ming leung Gilberto Ka-kit leung Kwok-Wing Chow Department of Mechanical Engineering University of Hong Kong Pokfulam Hong Kong Department of Neurosurgery Kwong Wah Hospital Hong Kong Division of Neurosurgery Department of Surgery Li Ka Shing Faculty of Medicine University of Hong Kong Queen Mary

Intracranial aneurysm, a localized dilation of arterial blood vessels in the Circle of Willis and its branches, is potentially life threatening, due to massive bleeding in the subarachnoid space upon rupture. In clinical practice, one minimally invasive surgical procedure is the implantation of a metallic stent to cover the aneurysm neck. This flow diverting device can reduce the flow into the aneurysm and enhance the prospect of thrombosis, a condition expected to reduce the risk of growth and rupture. The biomechanical and haemo-dynamic factors in stented and nonstented situations are studied by computational fluid dynamics. Unlike earlier models with straight or curved parent blood vessels, the aneurysm is now located near an arterial bifurcation. The influence of the aspect (depth to neck) ratio of the aneurysm on the flow dynamics will be emphasized, especially in the post-operation stages. More precisely, the maximum flow velocity, the variations of wall shear stress, the risk of stent migration and volumetric flow rate after endovascular treatment will be studied. Aneurysms with larger aspect ratios (i.e. smaller neck sizes for constant depth) generally pose a greater risk in terms of these flow parameters. These results will assist the applications and design of stents in future neurosurgical therapy. The approach is limited to a nonelastic model, without taking into account of questions like stent expansion and interaction with tissue.

关键词： Intracranial Aneurysm Endovascular Treatment Stent Aspect Ratio Computational Fluid Dynamics

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Regional trauma system development in Shenzhen,China:an 8-year journey

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Military Medical Research 2022年第3期9卷 385-386页

作者： Gui-Xi Zhang Gilberto Ka kit leung Chung Mau Lo Richard Kwong-Yin Lo John Wong Ronald V. Maier Eileen M.Bulger Joe King Man Fan Xiao-Bing Fu Chinese PLA Medical School Beijing100853China University of Hong Kong-Shenzhen Hospital Shenzhen518000China Faculty of Medicine University of Hong Kong Li Ka ShingHong Kong999077China Harborview Medical Center SeattleWA98195USA Medical Innovation Research Department and 4th Medical Center Chinese PLA General Hospital and Chinese PLA Medical SchoolBeijing100853China

Dear Editor,Since the late 1980s, trauma in China has been identified as a major public health challenge, with trafic-related fatalities accounting for 80% of accidental deaths[1]. In 2019, Shenzhen had a total population of approximately 20 million, and from 2010 to 2017, both emergency medical services and the total number of trauma patients increased, with trauma accounting for 47.0% and 38.4% of all patients in 2010 and 2017, respectively[2,3]. This report describes the efforts to implement programs and establish a trauma system in Shenzhen, China.

关键词： system services accounting

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芍药苷对雷西莫特诱导的巨噬细胞有氧糖酵解的影响

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中国临床药理学杂志 2024年第5期40卷 683-687页

作者：金莹莹时乐郝永熙唐璠蒋玟慧梁涛南京中医药大学药学院江苏南京210023

目的研究芍药苷对雷西莫特诱导的巨噬细胞有氧糖酵解的影响。方法用佛波酯(PMA)处理THP-1细胞使其分化成巨噬细胞。将细胞分为对照组、模型组和低、中、高剂量实验组。对照组细胞正常培养;模型组用2μg·mL^(-1)雷西莫特刺激24 h诱导... 详细信息

目的研究芍药苷对雷西莫特诱导的巨噬细胞有氧糖酵解的影响。方法用佛波酯(PMA)处理THP-1细胞使其分化成巨噬细胞。将细胞分为对照组、模型组和低、中、高剂量实验组。对照组细胞正常培养;模型组用2μg·mL^(-1)雷西莫特刺激24 h诱导巨噬细胞进行有氧糖酵解反应;低、中、高剂量实验组在模型组的基础上分别加入1、10和100μmol·L^(-1)芍药苷处理24 h。以细胞计数试剂盒-8(CCK-8)法检测细胞活性;以乳酸及葡萄糖测定试剂盒检测各组细胞乳酸的分泌和葡萄糖的消耗;以蛋白质印迹法和实时荧光定量聚核合酶链反应(q-PCR)法分别检测M2型丙酮酸激酶(PKM2)、乳酸脱氢酶A(LDHA)的蛋白和mRNA表达水平;以免疫荧光法比较各组细胞中PKM2的荧光强度。结果对照组、模型组和低、中、高剂量实验组细胞上清液中的葡萄糖含量分别为(14.70±0.44)、(9.83±0.43)、(10.68±0.29)、(11.79±0.33)和(13.63±0.74) mmol·L^(-1);细胞分泌的乳酸分别为(6.17±0.48)、(11.94±0.55)、(9.08±0.55)、(7.79±0.66)和(6.50±0.55) mmol·L^(-1);细胞中PKM2的蛋白表达水平分别为1.00±0.00、1.33±0.18、1.02±0.17、0.74±0.17和0.73±0.18;LDHA的蛋白表达水平分别为1.00±0.00、1.20±0.09、0.90±0.14、0.76±0.12和0.78±0.17;PKM2 mRNA表达水平分别为1.00±0.09、2.11±0.23、1.98±0.31、1.38±0.25和0.93±0.32;LDHA mRNA表达水平分别为1.00±0.13、1.85±0.25、1.44±0.21、0.91±0.24和0.96±0.14;PKM2的平均荧光强度分别为136.41±33.63、217.94±5.33、210.27±1.03、204.14±3.27和186.79±14.03。模型组上述指标与对照组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01);中、高剂量实验组上述指标与模型组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。结论芍药苷可以抑制R848诱导的巨噬细胞的有氧糖酵解。

关键词：芍药苷巨噬细胞 M2型丙酮酸激酶乳酸脱氢酶A 有氧糖酵解

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Risk Perception and Knowledge in Fire Risk Reduction in a Dong Minority Rural Village in China: A Health-EDRM Education Intervention Study

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International Journal of Disaster Risk Science 2018年第3期9卷 306-318页

作者： Emily ying Yang Chan Holly Ching Yu Lam Phoebe Pui Wun Chung Zhe Huang Tony Ka Chun Yung Kelvin Wai kit Ling Gloria Kwong Wai Chan Cheuk Pong Chiu Collaborating Centre for Oxford University and CUHK for Disaster and Medical Humanitarian Response (CCOUC) The Chinese University of Hong Kong Nuffield Department of Medicine University of Oxford The Jockey Club School of Public Health and Primary Care The Chinese University of Hong Kong Franois-Xavier Bagnoud Center for Health and Human Rights Harvard University Integrated Research on Disaster Risk (IRDR) International Centre of Excellence–CCOUC (ICoE-CCOUC)

Fire is one of the major disasters in rural communities but evidence of the effectiveness of education interventions against fire risks is limited. This was a 2-year study assessed the effectiveness of face-to-face Health Emergency and Disaster Risk Management(HealthEDRM) education interventions for raising fire risk reduction knowledge in a fire-prone rural ethnic minority community. The study was conducted in various pre-set time points of an intervention-based project in a Dongbased community in Nanjiang Village, Guizhou Province in 2015 and 2016 to increase knowledge among the villagers about how to reduce general-and electrical-fire risks. Pre-and post-intervention questionnaires were used to evaluate the effectiveness of increasing fire risk-related knowledge through these interventions, immediately after the 2015 and 2016 interventions, and 17 months after the 2015 intervention. The knowledge of using fire blanket,recalling the correct emergency telephone number,unplugging unused electrical appliances, and not using water to extinguish electrical fires had immediately improved after the interventions. Subjects demonstrated a better understanding that fire blankets can fight a blaze if used appropriately, and that knowledge was sustained for 17 months. The interventions were effective in improving fire prevention and response knowledge. Targeted interventions should be organized according to communities' culture, the evolution of economic prosperity and lifestyle practices.

关键词： Dong village Fire risk education Fire risk perception Fire risk reduction Health education Rural China Health-EDRM

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姜黄素介导的光动力疗法通过PI3K/AKT信号通路对PDGF-BB诱导的血管平滑肌细胞增殖的影响

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中华物理医学与康复杂志 2024年第5期46卷 393-400页

作者：王芳王改兰朱莹白定群重庆医科大学附属第一医院重庆医科大学附属第一医院康复医学科重庆400016

目的:观察姜黄素(CUR)介导的光动力疗法(PDT)通过磷酸酰肌醇3-激酶(PI3k)/丝苏氨酸蛋白激酶(AKT)信号通路对血小板衍生生长因子-BB(PDGF-BB)诱导的血管平滑肌细胞(VSMCs)增殖的影响及其可能的作用机制。方法:分别选取大鼠胸主动脉平滑... 详细信息

目的:观察姜黄素(CUR)介导的光动力疗法(PDT)通过磷酸酰肌醇3-激酶(PI3k)/丝苏氨酸蛋白激酶(AKT)信号通路对血小板衍生生长因子-BB(PDGF-BB)诱导的血管平滑肌细胞(VSMCs)增殖的影响及其可能的作用机制。方法:分别选取大鼠胸主动脉平滑肌细胞系(A7R5)和小鼠主动脉平滑肌细胞系(MOVAS)进行平行实验,选择20μg/L的PDGF-BB诱导VSMCs增殖。将培养好的VSMCs分为对照组、PDGF-BB组、姜黄素组、光动力组,其中光动力组再根据照射剂量的不同,又分为低剂量光动力组(照射60 s)、中剂量光动力组(照射120 s)、高剂量光动力组(照射180 s),共3个亚组。对照组不予任何刺激,PDGF-BB组饥饿过夜后加入含20μg/L的PDGF-BB的新鲜培养基刺激24 h,姜黄素组先给予与PDGF-BB组相同的干预,然后换液加入含20μmol/L CUR的新鲜培养基刺激6 h,光动力组先给予与姜黄素组相同的干预,再换液后采用425 nm激光对光动力组各亚组进行对应时长的激光照射。采用细胞活力检测法(CCK8)检测光动力组中不同激光剂量照射后,VSMCs的存活率;然后采用5-乙炔基-2′-脱氧尿苷(EDU)阳性标记率法检测4组细胞的增殖活性,采用流式细胞术对4组细胞进行细胞周期检测,采用Western blot法检测PI3K/AKT通路相关磷酸化蛋白和细胞增殖核蛋白(PCNA)的表达水平。结果:经CCK8法检测,本研究选择120 s作为激光照射的最佳剂量。干预后,中、高剂量光动力组的细胞存活率与PDGF-BB组比较,差异均有统计学意义(P<0.01)。光动力组增殖的阳性细胞比例显著低于PDGF-BB组,差异有统计学意义(P<0.05)。光动力组细胞在G0/G1期的比例明显低于对照组(P<0.01),而在G2/M期的比例则明显高于对照组和PDGF-BB组(P<0.01)。光动力组增殖细胞核抗原(PCNA)、PI3K/AKT通路磷酸化蛋白的表达较PDGF-BB组显著下降,差异均有统计学意义(P<0.01)。结论:CUR介导的PDT可抑制PDGF-BB诱导下的血管平滑肌细胞的增殖,其机制可能与CUR介导的PDT可下调PI3K/AKT通路相关磷酸化蛋白表达水平有关。

关键词：姜黄素光动力血小板衍生生长因子血管平滑肌细胞增殖

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信号芋螺内生真菌Talaromyces ***006次级代谢产物研究

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热带海洋学报 2024年第4期43卷 181-188页

作者：林应婷孙颖胡江南董帅热带生物资源教育部重点实验室药学院海南大学海南海口570228

文章旨在对信号芋螺内生真菌Talaromyces ***006的次级代谢产物进行结构和活性研究。运用多种分离材料和技术,如正反相硅胶柱层析、葡聚糖凝胶(sephadex LH-20)、聚苯乙烯反相树脂填料(MCI GEL CHP-20/P120)、半制备高效液相等进行分离... 详细信息

文章旨在对信号芋螺内生真菌Talaromyces ***006的次级代谢产物进行结构和活性研究。运用多种分离材料和技术,如正反相硅胶柱层析、葡聚糖凝胶(sephadex LH-20)、聚苯乙烯反相树脂填料(MCI GEL CHP-20/P120)、半制备高效液相等进行分离纯化,通过核磁共振、质谱等现代波谱技术进行结构鉴定。采用CCK8 (cell counting kit)法研究化合物1—14对脑胶质瘤细胞U87和肺癌细胞A549的抑制作用。从信号芋螺内生真菌Talaromyces ***006的次级代谢产物中分离获得14个化合物,分别是ergosteryl stearate (1)、麦角甾醇(2)、β-谷甾醇(3)、2-hydroxyemodin (4)、(+)-rugulosin (5)、1,1′,3,3′,5,5′-hexahydroxy-7,7-dimethyl[2,2′-bianthracene]-9,9′,10,10′-tetrone (6)、rel-(6R,7R)-5,6,7,8-tetrahydro-6-hydroxy-7-methyl-8-oxo-3-propyl-1H-2-benzopyran (7)、rel-(6R,7R)-5,6,7,8-tetrahydro-7-hydroxy-7-methyl-8-oxo-3-propyl-1H-2-benzopyran (8)、scorpinone (9)、orsellinic acid (10)、6-methyl-1,2,4-benzenetriol (11)、2-methyl-5-isopropyl-phenol (12)、3-methoxy-5-methyl-phenol (13)、5-methyl-benzene-1,3-diol (14)。化合物1—4和7—14为首次从Talaromyces sp.中分离获得。化合物8和12对脑胶质瘤细胞U87的半数抑制浓度(half maximal inhibitory concentration,IC50)分别为21.07μmol·L^(-1)和15.74μmol·L^(-1),同时对肺癌细胞A549的IC50分别为20.88μmol·L^(-1)和22.93μmol·L^(-1)。

关键词：信号芋螺内生菌篮状菌属次级代谢产物生物活性

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Diagnostic accuracy of Focused Abdominal Sonography for Trauma in blunt abdominal trauma patients in a trauma centre of Hong Kong

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Chinese Journal of Traumatology 2012年第5期15卷 273-278页

作者： Cheung Kent Shek Wong Hay Tai leung Ling Pong Tsang Tat Chi leung Gilberto Ka kit Department of Accident and Emergency Queen Mary Hospital 102 Pokfulam Road Pokfulam Hong Kong China Department of Surgery Li Ka Shing Faculty of Medicine University of Hong Kong Hong Kong China

Objective： Focused Abdominal Sono graphy for Trauma （FAST） is widely used for the detection of intraperitoneal free fluids in patients suffering from blunt abdominal trauma （BAT）. This study aimed at assessing the diagnostic accuracy of this investigation in a designated trauma centre. Methods： This was a retrospective study of BAT pa tients over a 6 year period seen in a trauma centre in Hong Kong. FAST findings were compared with laparotomy, ab dominal computed tomography or autopsy findings, which served as the gold standard for presence of intraperi toneal free fluids. The patients who did not have FAST or gold standard confirmatory test performed, had preexisting peritoneal fluid, died at resuscitation or had imcomplete docu mentation of FAST findings were excluded. The performance of FAST was expressed as sensitivity, specificity, predictive values （PV）, likelihood ratios （LR） and accuracy. Results： FAST was performed in 302 patients and 153 of them were included in this analysis. The sensitivity, specificity, positive PV, negative PV, positive LR, negative LR and accuracy for FAST were respectively 50.0%, 97.3%, 87.0%, 84.6%, 18.8, 0.5 and 85.0%. FAST was found to be more sensitive in less severely injured patients and more specific in more severely injured patients. Conclusion： FAST is a reliable investigation in the initial assessment of BAT patients. The diagnostic values of FAST could be affected by the severity of injury and staff training is needed to further enhance its effective use.

关键词： Laparotomy Autopsy Tomography, X-ray computed Tomography, spiral computed Ultra-sonography

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Genetic variability of CYP2B6 polymorphisms in four southern Chinese populations

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World Journal of Gastroenterology 2007年第14期13卷 2100-2103页

作者： Bing-ying Xu Li-Ping Guo Shui-Shan Lee Qing-Ming Dong Yi Tan Hong Yao Li-Hua Li Che-kit Lin Hsiang-Fu Kung Ming-Liang He Kunming Medical College Kunming 650032 Yunnan Province China Stanley Ho Center for Emerging Infectious Diseases and Li Ka Shing Institute of Health Sciences The Chinese University ofHong Kong Hong Kong China Department of Microbiology Faculty of Medicine The Chinese University of Hong Kong Hong Kong China Hong Kong Red Cross Blood Transfusion Service Hong Kong China

AIM： To investigate the genotype and allelic frequencies of Cytochrome P450 2B6 polymorphisms in four southern Chinese populations.METHODS： DNA was obtained from blood samples from Han Chinese from Hong Kong and three minority groups,the Wa, Bulang and Lahu from Yunnan in southern China. Genotyping was performed using real-time PCR and confirmed by direct sequencing.RESULTS： A total of 507 subjects from southern China were studied. Results showed there is a high prevalence of 516G 〉 T （34.5%） in ethnic Chinese compared to literature reports on other Asian populations and Caucasians. The frequency of the 516TT genotype is higher in the Hah majority （23.1%） than in three other ethnic minority groups （i.e., 7.4%, 9.1% and 15.8%） in southern China.CONCLUSION： This was the first study to document the spectrum of CYP2B6 allelic variants and genotypes in a southern Chinese population. The 516G 〉 T allele is associated with a defective metabolism of efavirenz （EFV）, which therefore may predispose to drug *** regimens for human immunodeficiency virus （HIV） and heroin addiction may need to be optimized in different populations because of the marked variability of the key metabolizing enzyme.

关键词： Polymorphism Allele Genotype Ethnicity Discrimination PCR

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N, N-dimethyltryptamine, a natural psychedelic, rapidly improves spatial memory and hippocampal longterm potentiation: a potential rapid-acting therapy for dementia-associated wandering

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中国药理学与毒理学杂志 2023年第7期37卷 489-490页

作者： CHENG Dan LEI Zhuogui Benjamin Chun-kit TONG Catherine Kin CHIU LI ying King-Ho CHEUNG ZHANG Zhangjin School of Chinese Medicine the University of HongKong Department of Neuroscience City University of HongKong School of Chinses Medicine and Mr. and Mrs. Ko Chi Ming Centre for Parkinson’s Disease Research HongKong Baptist University Department of Psychology LKS Faculty of Medicine the University of HongKong Department of Chinese Medicine the University of Hong Kong-Shenzhen Hospital

There is no fast-acting treatment strategies against Alzheimer′s disease(AD), in particular dementia-related wandering. N, N-dimethyltryptamine(DMT) is a natural psychedelic that may have rapid-onset nootropic effects. In this study, 5 × FAD transgenic mice which recapitulated amyloid neuropathological features of AD received one single injection of 6 or 12 mg · kg-1DMT and tested at 0.5, 1, and 2 h thereafter in Y-maze for spatial memory. 5×FAD transgenic mice exhibited pronounced decreases in time spent, number entered, and distance travelled in the novel arm of Y-maze. DMT at 12 mg · kg-1partially or completely reversed the three behavioral indices at multiple time points, up to 2 h post injection. The rapid-onset behavioral improvement was consistent with pharmacokinetic analysis of DMT, showing approximately 30 min to reach the maximum concentration in the brain tissue. The transgenic mice also displayed dramatically impaired hippocampal long-term potentiation(LTP), an electrophysiological feature of memory formation and consolidation. DMT potently enhanced LTP and restored intracellular calcium activity, expression and phosphorylation of calcium/calmodulin-dependent protein kinase Ⅱ(CaMKⅡ) and AMPA-type glutamate receptor1(GluR1), the two key calcium-activated mediators involved in LTP induction. Adenosine triphosphate(ATP)is purinergic signalling molecules that are involved in LTP induction and maintenance. DMT rapidly increased mitochondrial ATP dynamics in in vivo and in vitro *** results suggest that DMT rapidly improve spatial memory and hippocampal LTP by restoring the CaMKⅡ-GluR1 signaling pathway and mitochondrial ATP production. It may be served as a fast-acting nootropic agent for the treatment of AD in particular wandering.

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重组人源中期因子生物活性测定方法的建立与验证

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药学学报 2024年第1期59卷 198-201页

作者：张影贺婷邱明丰彭生斌上海交通大学药学院上海200240 祐森健恒生物医药(上海)有限公司上海201203

建立和优化重组人源中期因子(recombinant human midkine,rhMK)活性检测方法,并对其方法进行验证。本文采用细胞计数试剂盒-8(cell counting kit-8,cck-8)法检测rhMK作用大鼠膝软骨细胞的促增殖活性,并对方法进行专属性、准确性、精密... 详细信息

建立和优化重组人源中期因子(recombinant human midkine,rhMK)活性检测方法,并对其方法进行验证。本文采用细胞计数试剂盒-8(cell counting kit-8,cck-8)法检测rhMK作用大鼠膝软骨细胞的促增殖活性,并对方法进行专属性、准确性、精密性、线性、耐用性验证。RhMK的缓冲液及重组人源白介素-1受体拮抗剂无明显活性效力,该方法专属性良好;相对活性为50%、75%、100%、125%、150%的样品效价回收率(n=6)统计在80.0%~124.0%之间,各相对效价的相对标准偏差均在20%以内,线性相关系数R^(2)≥0.98,表明方法的准确性、精密性、线性均良好;耐用性相关系数R^(2)≥0.92,量效曲线的最大值与最小值的比值≥1.5,方法的耐用性良好。研究建立的rhMK的生物学活性检测方法,其方法验证良好,为rhMK开发过程中常规样品的生物学活性分析提供了可靠的检测方法。本研究已获得上海南方模式生物科技股份有限公司动物看护与使用委员会批准(批准号:2019-0008-06)。

关键词：重组人源中期因子 cck-8法生物学活性增殖方法验证

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