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Biomedical and Environmental Sciences 2017年第8期30卷 611-615页

作者： BAI Juan ZHU ying DONG ying School of Food and Biological Engineering Jiangsu University Zhenjiang 212013 Jiangsu China

This study explored how bitter melon powder （BMP） alters the colonic microenvironment during the development of obesity-associated fatty liver in rats. We observed that BMP effectively inhibited the body weight gain and lipid accumulation in the liver, ameliorated glucose intolerance, and increased the colon weight after an 8-week treatment compared to that in the high-fat diet （HFD） group. BMP significantly decreased fecal water toxicity towards HT-29 cells, as revealed by the cell counting kit （CCK）-8 assay results, and the mRNA expression of Toll-like receptor 4 （TLR4） in colon mucosa. Additionally, gut permeability in the BMP group was restored to normal levels. Finally, BMP alleviated the inflammatory state of the rat colon mucosa and liver tissues as well as the systemic inflammation.

关键词： RNA Bitter Melon Powder Protects against Obesity-associated Fatty Liver Disease by Improving Colonic Microenvironment in Rats with High-fat Diet-induced Obesity BMP Figure TLR

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儿童呼吸系统鼻病毒感染流行特征分析

中国病原生物学杂志 2024年第3期19卷 312-315页

作者：常颖黄光举张慧玉李娟徐静唐山市妇幼保健院小儿呼吸科河北唐山063000

目的分析唐山市儿童感染鼻病毒的流行特点。方法收集2021年11月到2022年7月本院1500例就诊儿童资料,收集患儿痰液标本、咽拭子标本、肺泡灌洗液标本,并采用肺炎多重检验试剂盒进行病原鉴定,采用SPSS软件比较不同年龄和型别患儿鼻病毒阳... 详细信息

目的分析唐山市儿童感染鼻病毒的流行特点。方法收集2021年11月到2022年7月本院1500例就诊儿童资料,收集患儿痰液标本、咽拭子标本、肺泡灌洗液标本,并采用肺炎多重检验试剂盒进行病原鉴定,采用SPSS软件比较不同年龄和型别患儿鼻病毒阳性率差异。选取30份鼻病毒阳性标本,并进行VP4/VP2区扩增和测序。结果共计检出309例(309/1500,20.60%)患儿携带鼻病毒,其中男186例(186/826,22.52%),女123例(123/674,18.25%),两组患儿鼻病毒阳性检出率差异有统计学意义(χ^(2)=4.1352,P<0.05)。<1岁组、1~岁组、3~岁组和≥6岁组,各组患儿鼻病毒阳性率分别为:23.39%(102/436)、21.85%(137/627)、19.56%(23/271)和10.24%(17/166),≥6组患儿与其他组患儿鼻病毒阳性检出率差异有统计学意义(χ^(2)=13.1154、11.3038和6.6416,均P<0.05)。共有142例(142/309,45.95%)患儿为混合感染,其中双重感染120例,三重感染22例。双重感染中RV+RSV A型检出最多,其次是RV+ADV型。三重感染中RV+RSV A+ADV型、RV+ADV+EV型和RV+PIV3+MP型检出较多。30株鼻病毒经分子分型鉴定结果显示21株RV A型,3株RV B型和6株RV C型。A型共检出9个基因型,B型共检出1个基因型,C型共检出2个基因型。结论本次研究中<1岁组RV阳性率最高,随着年龄增长,RV检出率下降。鼻病毒与呼吸道合胞病毒、腺病毒和副流感病毒合并感染病例较多。鼻病毒A型是本次研究中优势株。

关键词：儿童鼻病毒流行特征

甲壳类和贝类原肌球蛋白的广谱单克隆抗体制备与鉴定

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上海海洋大学学报 2024年第2期33卷 389-397页

作者：陆佳达栾宏伟闫艺方施文正卢瑛上海海洋大学食品学院上海201306 上海海洋大学水产品加工及贮藏工程技术研究中心上海201306 上海海洋大学农业农村部水产品贮藏保鲜质量安全风险评估实验室上海201306

原肌球蛋白(Tropomyosin,TM)被认为是甲壳类和贝类的主要过敏原,为了应对与日俱增的甲壳类和贝类过敏风险,以凡纳滨对虾的TM富集液为抗原免疫BALB/c小鼠,通过杂交瘤细胞融合技术筛选获得1株凡纳滨对虾过敏原TM的单克隆抗体细胞株2F8,并... 详细信息

原肌球蛋白(Tropomyosin,TM)被认为是甲壳类和贝类的主要过敏原,为了应对与日俱增的甲壳类和贝类过敏风险,以凡纳滨对虾的TM富集液为抗原免疫BALB/c小鼠,通过杂交瘤细胞融合技术筛选获得1株凡纳滨对虾过敏原TM的单克隆抗体细胞株2F8,并利用ELISA、Western blot、生物信息学等方法对2F8单抗进行免疫学特性分析,确定其效价、特异性、抗体亚型、结合区域,以TM的特异性单抗2F8和兔多抗构建双抗夹心胶体金试纸条分析单抗2F8的应用可行性。结果显示,单抗2F8的抗体亚型为IgG1型、效价为1.28×10^(5),抗体与TM抗原的IC_(50)值为20.21,亲和力高;纯化的单抗2F8可以特异性识别甲壳类、贝类和鱿鱼的主要过敏原TM,存在3个潜在的TM抗原结合区域AA_(0-31),AA_(38-43)和AA_(58-67)。制备的试纸条检测结果显示,该试纸条可在10 min内目测5 ng/mL的凡纳滨对虾TM(相当于12.5μg/kg),且可用于甲壳类和贝类肌肉中TM的检测,研究表明所制备的2F8单抗亲和力高,可用于虾蟹贝和鱿鱼中的过敏原TM的鉴定与检测,具有较好的应用价值。本研究为过敏原TM的鉴定和检测提供了重要生物材料,也为今后TM的快速检测试剂盒开发提供了技术支持。

关键词：原肌球蛋白单克隆抗体凡纳滨对虾甲壳类贝类

复合材料Ⅰ形梁分层的光纤超声探测技术数值研究

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复合材料学报 2010年第3期27卷 162-168页

作者：许颖喻毅梁坚凝哈尔滨工业大学深圳研究生院深圳518055 香港科技大学土木工程系

针对纤维增强塑料多界面、高阻尼以及体波模式多样性对超声无损检测的影响,介绍了一种利用光纤超声探测技术结合小波信号分析检测I形纤维增强塑料梁中分层的方法。该方法利用超声发射器在梁中产生超声应力波,用表面粘贴的光纤干涉仪来... 详细信息

针对纤维增强塑料多界面、高阻尼以及体波模式多样性对超声无损检测的影响,介绍了一种利用光纤超声探测技术结合小波信号分析检测I形纤维增强塑料梁中分层的方法。该方法利用超声发射器在梁中产生超声应力波,用表面粘贴的光纤干涉仪来探测梁中应力波的传播情况,然后利用小波的多分辨率特性对此信号进行时频分析,结合梁中超声波的传播特性即可确定分层的位置。理论分析和模拟结果都表明了此方法探测复合材料梁腹板/翼缘连接处分层的可行性。

关键词：光纤干涉仪分层复合材料超声波小波分析

Role of ion channels in maintaining the characteristics of embryonic stem cells

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Role of ion channels in maintaining the characteristics of e...

老年心力衰竭合并肺部感染患者病原菌分布及血清IL-8、PTX3水平变化

第七届海峡两岸心血管科学研讨会

作者： Sze-ying Ng Yuen-Ting Lau Chi-Hou Chin Chun-kit Wong Suk-ying Tsang Department of Biochemistry Chinese University of Hong Kong

Embryonic stem cells(ESCs)are derived from the inner cell mass of *** can self-renew and are pluripotent,meaning that they can proliferate indefinitely and have the ability to differentiate into all cells in our *** may therefore act as a source of different cell types for future regenerative *** the present study,the role of voltage-gated K(Kv)channels in maintaining the self-renewal and pluripotent characteristics of undifferentiated mouse(m)ESCs was *** channel blocker tetraethylammonium(TEA)was shown to decrease mESC proliferation without affecting cell *** of Kv channels resulted in cell cycle arrest at the G/G *** addition,decreased expression of pluripotent markers such as Oct-4 was detected in Kv channel -blocked *** Kv blockade led to depolarization,and Kv channel-blocked ESCs failed to progress through the cell cycle and had a loss of pluripotency,the present results suggested a possible relationship between the Kv channel functions,membrane potential,cell cycle progression and differentiation potential in *** study shall provide new information about the basic biology of ESCs and provide insights into the possible strategies of controlling ESC self-renewal and differentiation.

关键词：

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中国病原生物学杂志 2024年第6期19卷 724-728页

作者：杨海燕刘英曹艳华黄燕红上海市浦东医院(复旦大学附属浦东医院) 上海201399

目的探讨老年心力衰竭合并肺部感染患者病原菌分布及血清白介素-8(IL-8)、正五聚蛋白3(PTX3)水平变化。方法回顾性收集本院2022年11月-2023年12月接诊的120例心力衰竭合并肺部感染老年患者作为观察组,收集同期本院体检健康者132例作为... 详细信息

目的探讨老年心力衰竭合并肺部感染患者病原菌分布及血清白介素-8(IL-8)、正五聚蛋白3(PTX3)水平变化。方法回顾性收集本院2022年11月-2023年12月接诊的120例心力衰竭合并肺部感染老年患者作为观察组,收集同期本院体检健康者132例作为对照组,采用ELISA试剂盒检测血清IL-8、PTX3水平;患者治疗前收集呼吸道分泌物标本,培养病原体并进行药敏试验;根据患者的预后分为生存组(n=105)和死亡组(n=15),Logistic分析患者预后的影响因素;绘制ROC曲线分析血清IL-8、PTX3水平对老年心力衰竭合并肺部感染患者预后的诊断价值。结果患者的呼吸道分泌物标本培养病原菌共112株,其中革兰阳性菌26株(23.21%),革兰阴性菌76株(67.86%),真菌10株(8.93%);观察组较对照组血清IL-8[(13.22±2.07)ng/L vs(7.42±1.42)ng/L]、PTX3水平[(11.36±1.44)ng/L vs(6.25±1.03)ng/L]均升高(P<0.05);死亡组较生存组血清IL-8[(19.34±2.34)ng/L vs(12.35±2.03)ng/L]、PTX3水平[(18.34±2.03)ng/L vs(10.36±1.36)ng/L]均升高(P<0.05);Logistic回归分析得知PIL-8、PTX3是患者预后的危险因素(P<0.05)。血清IL-8、PTX3单独及联合诊断患者发生死亡的AUC分别为0.819、0.792、0.866,二者联合诊断的AUC高于单独诊断(Z=2.068、2.013,P=0.024、0.026)。结论老年心力衰竭合并肺部感染患者血清IL-8、PTX3水平显著升高,感染病原菌以革兰阴性菌为主,IL-8、PTX3过表达可能增加死亡风险,定期监测血清IL-8、PTX3水平变化可能对减少预后不良的发生有积极作用。

关键词：老年心力衰竭合并肺部感染病原菌白介素-8 正五聚蛋白3 预后

Coal-based energychemicalengineering:Pay one’srespectsto ***

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Chinese Journal of Chemical Engineering 2021年第7期34卷 1-2页

作者： Wen-ying Li Jinlong Gong Taiyuan University of Technology China Tianjin University China

Coal is of utmost importance for the energy and chemical engi-neering fields of China,as it contributes to 56.8%of primary energy supply in 2020 in the *** the one hand,coal is the major source of energy and resources for its rich endowment in China;However,on the other hand,it is the major source of environmen-tal pollution due to the implementation of outdated ***,according to Professor Kechang Xie in 2004,“The only way out is to develop and promote the clean and efficient coal uti-lization technologies”.In the past 40 years,China has made a sig-nificant progress in research and development related to modern coal chemical engineering,including lab-scale fundamental research and industrial-scale construction of many giant chemical plants.

关键词： energy chemical dated

用光纤干涉仪检测I形复合材料梁腹板/翼缘连接处的分层

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复合材料学报 2007年第2期24卷 143-150页

作者：许颖梁坚凝江毅深圳市建筑科学研究院香港科技大学土木工程系北京理工大学光电工程系北京100081

介绍了一种在超声回弹波频谱分析基础上用光纤干涉仪来检测I形复合材料梁腹板/翼缘连接处分层的方法,利用超声发射器在I形梁中产生应力波,用表面粘贴的光纤干涉仪来接收应力波产生的输出信号,对此信号进行频谱分析可找到I形梁的分层位... 详细信息

介绍了一种在超声回弹波频谱分析基础上用光纤干涉仪来检测I形复合材料梁腹板/翼缘连接处分层的方法,利用超声发射器在I形梁中产生应力波,用表面粘贴的光纤干涉仪来接收应力波产生的输出信号,对此信号进行频谱分析可找到I形梁的分层位置。理论分析和实验都表明了此方法探测复合材料梁腹板/翼缘连接处分层的可行性。

关键词： Mach—Zehnder光纤干涉仪分层复合材料腹板脉冲回波

Roles of Serum Amyloid A 1 Protein Isoforms in Rheumatoid Arthritis

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Engineering 2022年第3期8卷 174-182页

作者： Elaine Laihan leung Huan-Ling Lai Run-Ze Li Hu-Dan Pan Ze-Bo Jiang ying Li Fu-Gang Duan Jia-Hui Xu Yi-Zhong Zhang A-Xi Shi Chun-Li Wei Fang-Yuan Zhang Xiao-Jun Yao Liang Liu State Key Laboratory of Quality Research in Chinese Medicine&Macao Institute for Applied Research in Medicine and Health&Faculty of Chinese Medicine Macao University of Science and TechnologyMacao 999078China Department of Pediatrics Pennsylvania State University College of MedicineHersheyPA 17033USA

Secondary amyloid A amyloidosis,a lethal complication,is induced when acute or chronic infection coexists with over-secretion of the serum amyloid A 1(SAA1)protein and deposition in key internal ***,using the whole-exome sequencing method,we identified a novel deleterious mutation SAA1.2 in rheumatoid arthritis(RA)***,the role of SAA1 in RA pathogenesis and its complications remains *** purpose of this study was to determine the pathogenetic roles of SAA1 protein isoforms in RA *** modified an experimental adenovirus infection protocol to introduce SAA1.2 gene alleles into the knee joints of mice and used SAA1.3 and SAA1.5 as *** tomography analysis was applied to determine changes in bone morphology and ***(IHC)analysis,flow cytometry,enzyme-linked immunosorbent assay(ELISA),and real-time polymerase chain reaction(RT-PCR)were used to investigate disease progression and cytokine alterations in the course of adenoviral SAA-induced knee joint inflammation and bone *** found that the arthritis-inducing effect of SAA1.2 transcription in the knee joints and mutant SAA1 protein secretion in blood resulted in the stimulation of immune responses,leading to CD8^(+)T cell and pro-inflammatory cytokine elevation,such as interleukin(IL)-6,IL-22,matrix metalloproteinase(MMP)-3,MMP-9,with subsequent synovial inflammation and bone *** findings indicate that SAA1 protein isoforms,particularly SAA1.2,play a significant role in the induction and progression of RA and may have potential value in the early diagnosis and severity prediction of RA.

关键词： Serum amyloid A 1 Rheumatoid arthritis Pathogenetic Intra-articular

miR-7/TGF-β2 axis sustains acidic tumor microenvironment-induced lung cancer metastasis

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Acta Pharmaceutica Sinica B 2022年第2期12卷 821-837页

作者： Tao Su Suchao Huang Yanmin Zhang Yajuan Guo Shuwei Zhang Jiaji Guan Mingjing Meng Linxin Liu Caiyan Wang Dihua Yu Hiu-Yee Kwan Zhiying Huang Qiuju Huang Elaine Lai-Han leung Ming Hu ying Wang Zhongqiu Liu Linlin Lu Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People’s Republic of China Guangzhou University of Chinese MedicineGuangzhou 510006China Department of Molecular and Cellular Oncology the University of Texas MD Anderson Cancer CenterHoustonTX 77030USA School of Chinese Medicine Hong Kong Baptist UniversityHong Kong 999077China State Key Laboratory of Quality Research in Chinese Medicine/Macao Institute for Applied Research in Medicine and Health Macao University of Science and TechnologyMacao(SAR)999078China Department of Pharmacological and Pharmaceutical Sciences University of HoustonHoustonTX 77204USA

Acidosis,regardless of hypoxia involvement,is recognized as a chronic and harsh tumor microenvironment(TME)that educates malignant cells to thrive and *** overwhelming evidence supports an acidic environment as a driver or ubiquitous hallmark of cancer progression,the unrevealed core mechanisms underlying the direct effect of acidification on tumorigenesis have hindered the discovery of novel therapeutic targets and clinical ***,chemical-induced and transgenic mouse models for colon,liver and lung cancer were established,***-7 and TGF-β2 expressions were examined in clinical tissues(n=184).RNA-seq,miRNA-seq,proteomics,biosynthesis analyses and functional studies were performed to validate the mechanisms involved in the acidic TME-induced lung cancer *** data show that lung cancer is sensitive to the increased acidification of TME,and acidic TME-induced lung cancer metastasis via inhibition of miR-7-5 ***-β2 is a direct target of miR-7-5 *** reduced expression of miR-7-5 p subsequently increases the expression of TGF-β2 which enhances the metastatic potential of the lung ***,overexpression of miR-7-5 p reduces the acidic p H-enhanced lung cancer ***,the human lung tumor samples also show a reduced miR-7-5 p expression but an elevated level of activated TGF-β2;the expressions of both miR-7-5 p and TGF-β2 are correlated with patients’*** are the first to identify the role of the miR-7/TGF-β2 axis in acidic p H-enhanced lung cancer *** study not only delineates how acidification directly affects tumorigenesis,but also suggests miR-7 is a novel reliable biomarker for acidic TME and a novel therapeutic target for non-small cell lung cancer(NSCLC)*** study opens an avenue to explore the p H-sensitive subcellular components as novel therapeutic targets for cancer treatment.

关键词： Acidic tumor microenvironment miR-7-5p TGF-β2 Metastasis Lung cancer pH Invasion