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Desmoglein 2(DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia

Virologica Sinica 2021年第6期36卷 1400-1410页

作者： Jing Zhang Kui Ma Xiangyu Wang yinbo jiang Shan Zhao Junxian Ou Wendong Lan Wenyi Guan Xiaowei Wu Heping Zheng Bin Yang Chengsong Wan Wei Zhao jianguo Wu Qiwei Zhang Guangdong Provincial Key Laboratory of Virology Institute of Medical MicrobiologyJinan UniversityGuangzhou510632China Guangdong Provincial Key Laboratory of Tropical Disease Research School of Public HealthSouthern Medical UniversityGuangzhou510515China Guangdong Provincial Dermatology Hospital Southern Medical UniversityGuangzhou510091China

Human adenovirus type 55(HAdV-B55) is a re-emergent acute respiratory disease pathogen that causes adult communityacquired pneumonia(CAP). Previous studies have shown that the receptor of HAdV-B14, which genome is highly similar with HAdV-B55, is human Desmoglein 2(DSG2). However, whether the receptor of HAdV-B55 is DSG2 is undetermined because there are three amino acid mutations in the fiber gene between HAdV-B14 and HAdV-B55. Here, firstly we found the 3T3 cells, a mouse embryo fibroblast rodent cell line which does not express human DSG2, were able to be infected by HAdV-B55 after transfected with pcDNA3.1-DSG2, while normal 3T3 cells were still unsusceptible to HAdV-B55 infection. Next, A549 cells with h DSG2 knock-down by siRNA were hard to be infected by HAdV-B3/-B14/-B55, while the control siRNA group was still able to be infected by all these types of HAdVs. Finally, immunofluorescence confocal microscopy indicated visually that Cy3-conjugated HAdV-B55 viruses entered A549 cells by binding to DSG2 ***, DSG2 is a major receptor of HAdV-B55 causing adult CAP. Our finding is important for better understanding of interactions between adenoviruses and host cells and may shed light on the development of new drugs that can interfere with these processes as well as for the development of potent prophylactic vaccines.

关键词： Human adenovirus type 55(HAdV-B55) Severe community-acquired pneumonia Adenovirus receptor Desmoglein 2(DSG2)

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基于组织因素的高校学术不端行为影响因素的敏感性分析

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管理学报 2009年第2期6卷 264-270页

作者：常亚平蒋音播阎俊华中科技大学管理学院

从组织因素的角度出发,应用多元统计分析的手段,对我国高校学者学术不端行为的影响因子进行了研究。研究发现,学校声誉、领导者对科研重视度、学校内部监督健全度和组织内学术道德风气是影响学术不端行为的显著因素。同时发现:不同类别... 详细信息

从组织因素的角度出发,应用多元统计分析的手段,对我国高校学者学术不端行为的影响因子进行了研究。研究发现,学校声誉、领导者对科研重视度、学校内部监督健全度和组织内学术道德风气是影响学术不端行为的显著因素。同时发现:不同类别学校的学者在学术不端行为上存在显著差异。针对这些显著性因素提出了政策建议。

关键词：高校学术不端影响因素敏感性

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随钻堵漏用疏水缔合聚合物的作用机理分析

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钻井液与完井液 2017年第1期34卷 50-53,59页

作者：蒋官澄刘冲贺垠博蒋其辉王春蕾葛庆颖赵利中国石油大学(北京)石油工程学院北京102249 西部钻探工程有限公司克拉玛依钻井公司新疆克拉玛依834000

针对常规堵漏材料难以对非均质强的渗透地层实现随钻封堵的难题,以长碳链烷基二甲基烯丙基氯化铵、丙烯酰胺、N-乙烯基吡咯烷酮和丙烯酸为单体,通过调整引发剂比例和种类及反应条件后,合成了一种随钻堵漏用疏水缔合聚合物JD。通过红外... 详细信息

针对常规堵漏材料难以对非均质强的渗透地层实现随钻封堵的难题,以长碳链烷基二甲基烯丙基氯化铵、丙烯酰胺、N-乙烯基吡咯烷酮和丙烯酸为单体,通过调整引发剂比例和种类及反应条件后,合成了一种随钻堵漏用疏水缔合聚合物JD。通过红外光谱表征,合成产物为4种单体的共聚物。分别对0.3%和0.6%的JD溶液及加有0.3%JD的4%膨润土浆进行透射电镜分析表明,聚合物分子之间通过疏水缔合形成了类似胶束的疏水缔合结构,其尺寸约为0.1~0.2μm,同时疏水缔合聚合物与膨润土之间形成了动态网架结构,这正是其具有优良封堵性能的原因。通过使用FEIQuanta200F场发射环境扫描电镜测试得到,加入JD后的滤饼表面有大量聚合物形成的网架结构。静态封堵结果表明,在钻井液体系中加入0.3%JD,粒径为0.45~0.90 mm砂床的漏失量降低82%,粒径为0.22~0.45 mm与0.12~0.22 mm砂床可完全封堵,即JD可对不同漏失情况的砂床实现有效封堵,使钻井液可以随钻封堵非均质性渗透地层。配伍性评价表明,JD与SMP-Ⅱ、SPNH、聚合醇有良好的复配效果,与Redu1、NH4-HPAN、乳化沥青复配黏度和切力有所增大,因此JD更适用于黏度和切力不太高的体系。

关键词：防漏堵漏水基钻井液疏水缔合聚合物微观结构

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MF微生物驱油先导试验

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海洋石油 2007年第3期27卷 98-101页

作者：马香丽高翔郑卫宏刘明峰江银波吴立滨中原油田分公司采油二厂河南范县457532

沙二上2+3油藏为高渗油藏,随着长期注水开发,由于层内非均质性增强,水淹快,采出程度低,层系开发的层内矛盾加剧。分析历年驱油存在的问题,发现常用的驱油技术(聚合物驱,表面活性剂驱,碱驱,复合驱)适应性变差,深度有限,只能提高一定的波... 详细信息

沙二上2+3油藏为高渗油藏,随着长期注水开发,由于层内非均质性增强,水淹快,采出程度低,层系开发的层内矛盾加剧。分析历年驱油存在的问题,发现常用的驱油技术(聚合物驱,表面活性剂驱,碱驱,复合驱)适应性变差,深度有限,只能提高一定的波及系数,而不能提高洗油能力,随着驱油轮次的增加,已不能达到预期的效果。MF微生物是一种新型的驱油剂,MF微生物驱油技术是结合生物聚合物和EPS的研究成果,开发的一项提高采收率的新技术。室内试验表明,MF微生物在注入水环境中生长繁殖良好,并对硫酸盐还原菌有较强的抑制作用。2006年在沙二上2+3油藏的2个井组进行先导试验,对应的8口油井见到很好的增油效果,累积增油2 448 t,具有广阔的应用前景。

关键词：非均质驱油 MF微生物增油

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沙眼衣原体主要外膜蛋白多肽多克隆抗体的制备与鉴定

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实用医学杂志 2022年第11期38卷 1333-1338页

作者：徐青青陈文韬江银波蓝银苑潘钰莹曾丽红杨建江薛耀华郑和平安徽医科大学广东皮肤病临床学院合肥230032 广东省皮肤病医院广州510091 南方医科大学皮肤病医院广州510091

目的沙眼衣原体主要外膜蛋白(major outer membrane protein,MOMP)在维持沙眼衣原体结构稳定性和衣原体感染宿主细胞过程中发挥重要作用,为深入研究MOMP蛋白的功能及作用机制,使用人工合成多肽制备MOMP多克隆抗体。方法分析沙眼衣原体... 详细信息

目的沙眼衣原体主要外膜蛋白(major outer membrane protein,MOMP)在维持沙眼衣原体结构稳定性和衣原体感染宿主细胞过程中发挥重要作用,为深入研究MOMP蛋白的功能及作用机制,使用人工合成多肽制备MOMP多克隆抗体。方法分析沙眼衣原体MOMP的抗原表位,根据15个沙眼衣原体血清型MOMP保守肽段,设计并合成MOMP特异性肽段,与血蓝蛋白偶联后免疫新西兰兔。采用免疫亲和层析纯化免疫血清,应用SDS-PAGE和ELISA评估血清抗体纯度及效价,利用Western blot和免疫荧光染色鉴定MOMP抗体特异性。结果纯化后的血清IgG抗体纯度为89.6%,MOMP抗体效价达1∶12 800,Western blot结果显示抗体与8个血清型沙眼衣原体蛋白在40 kDa处特异性结合,免疫荧光染色证实该抗体可特异性结合沙眼衣原体。此外,MOMP抗体与进口商品化抗体明显共定位于宿主细胞内,且两种抗体荧光强度相当。结论本研究成功制备效价高、特异性好的沙眼衣原体MOMP的兔多克隆抗体,为后续深入研究MOMP的生物学功能提供了有力工具。

关键词：沙眼衣原体主要外膜蛋白多肽多克隆抗体

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Novel Water-Based Drilling and Completion Fluid Technology to Improve Wellbore Quality During Drilling and Protect Unconventional Reservoirs

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Engineering 2022年第11期18卷 129-142页

作者： Guancheng jiang Jinsheng Sun yinbo He Kaixiao Cui Tengfei Dong Lili Yang Xukun Yang Xingxing Wang State Key Laboratory of Petroleum Resources and Prospecting MOE Key Laboratory of Petroleum EngineeringChina University of Petroleum(Beijing)Beijing 102249China School of Petroleum Engineering China University of Petroleum(East China)Qingdao 266850China

The efficient exploration and development of unconventional oil and gas are critical for increasing the self-sufficiency of oil and gas supplies in ***,such operations continue to face serious problems(e.g.,borehole collapse,loss,and high friction),and associated formation damage can severely impact well completion rates,increase costs,and reduce ***-based drilling fluids possess certain advantages over oil-based drilling fluids(OBDFs)and may offer lasting solutions to resolve the aforementioned ***,a significant breakthrough with this material has not yet been made,and major technical problems continue to hinder the economic and large-scale development of unconventional oil and ***,the international frontier external method,which only improves drilling fluid inhibition and lubricity,is expanded into an internal-external technique that improves the overall wellbore quality during *** technologies are introduced into the chemical material synthesis process to imitate the activity of life.A novel drilling and completion fluid technique was developed to improve wellbore quality during drilling and safeguard formation *** and microscopic analyses indicated that in terms of wellbore stability,lubricity,and formation protection,this approach could outperform methods that use typical *** proposed method also achieves a classification upgrade from environmentally protective drilling fluid to an ecologically friendly drilling *** developed technology was verified in more than 1000 unconventional oil and gas wells in China,and the results indicate significant alleviation of the formation damage attributed to borehole collapse,loss,and high *** has been recognized as an effective core technology for exploiting unconventional oil and gas *** study introduces a novel research direction for formation protection technology and demonstrates that observations and learning from the n

关键词： Formation protection Wellbore quality Unconventional oil and gas Drilling and completion fluid Bionics

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The biodiesel-based flat-rheology drilling fluid system

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Petroleum Exploration and Development 2022年第1期49卷 200-210页

作者： jiang Guancheng SHI He HE yinbo Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum(Beijing)Beijing 102249China MOE Key Laboratory of Petroleum Engineering China University of Petroleum(Beijing)Beijing 102249China CNPC Engineering Technology R&D Company Limited Beijing 102206China

As the base oil of the current flat-rheology synthetic drilling fluid is high in cost and not renewable, the biodiesel-based flat-rheology drilling fluid with low-cost, environmental protection and renewable advantage was studied. Based on the optimization of raw materials, a cheap, environment-friendly biodiesel of soybean oil ethyl ester with good fluidity at low temperature was selected as the base oil. By selecting high oil-water ratio and introducing cationic surfactant into the auxiliary emulsifier, the thickening of biodiesel-based emulsion caused by hydrolysis and saponification after high-temperature aging was effectively eliminated. The organoclay prepared with cationic modifier of hexadecyl trimethyl ammonium chloride was used to improve the rheologic properties, stability and fluid loss of the drilling fluid while preventing low-temperature thickening. A flat-rheology modifier was synthesized with dimer fatty acid and cocoanut fatty acid diethanolamide, which could form strong network structure in the biodiesel-based drilling fluid to adjust effectively rheological properties of the drilling fluid. A biodiesel-based flat-rheology drilling fluid system with the density of 1.2 g/cm^(3) has been formulated which has constant rheology in the temperature range of 2-90 ℃, temperature tolerance of 160 ℃, seawater salinity tolerance of 5%, shale cuttings tolerance of 10%, and is environmentally friendly.

关键词： biodiesel flat-rheology drilling fluid emulsifier organoclay rheology modifier environment friendly deep water drilling

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Stimulus-responsive mechanism of salt-responsive polymer and its application in saturated saltwater drilling fluid

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Petroleum Exploration and Development 2020年第5期47卷 1131-1137页

作者： HE yinbo jiang Guancheng DONG Tengfei YANG Lili LI Xiaoqing State Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum(Beijing)Beijing 102249China MOE Key Laboratory of Petroleum Engineering China University of Petroleum(Beijing)Beijing 102249China

AM-AMPS-TAC polymers with different charge distribution are synthesized using acrylamide(AM),2-acrylamido-2-methylpropanesulfonate(AMPS)and 3-acrylamidopropyl trimethylammonium(TAC)at different feed ratios by polymerization in *** salt-responsive behavior,reasons leading to salt-responsiveness,and effects of polymers molecular structure on salt-responsiveness are studied by laboratory experiments to find out the adaptability of the *** test under stepwise shear mode shows that the AM-AMPS-TAC polymers have salt responsiveness,and the closer the feeds of AMPS and ATC,the more significant the salt responsiveness will *** change of polymers molecular chain under salt stimulus is studied by turbidity test and micro-morphology analysis,and the responsive mechanism is further investigated by intrinsic viscosity test and copolymer composition *** indicate that the salt-responsive behavior of AM-AMPS-TAC polymers derives from the"curled to expanded"transition of chain conformation under salt stimulus,and this transition is led by the screening effect of salt which weakens polymers intramolecular ionic *** in saturated saltwater drilling fluid shows that the AM90-AMPS5-TAC5 polymer has the best salt-tolerance and temperature-tolerance when used together with fluid loss controller *** drilling fluid saturated with NaCl can maintain stable viscosity,good dispersion and low fluid loss for long time under 150℃.

关键词： salt-responsive polymer the salt-responsive behavior stimulus-responsive mechanism chain conformation polymer additive saturated saltwater drilling fluid

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Nuclear translocation of OsMADS25 facilitated by OsNAR2.1 in reponse to nitrate signals promotes rice root growth by targeting OsMADS27 and OsARF7

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Plant Communications 2023年第6期4卷 329-341页

作者： Junyu Wu Shuaiqi Yang Nana Chen Qining jiang Linli Huang Jiaxuan Qi Guohua Xu Lisha Shen Hao Yu Xiaorong Fan yinbo Gan Zhejiang Key Laboratory of Crop Germplasm Department of AgronomyCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310000China State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095China Temasek Life Sciences Laboratory and Department of Biological Sciences National University of Singapore1 Research LinkSingapore 117604Singapore

Nitrate is an important nitrogen source and signaling molecule that regulates plant growth and *** several components of the nitrate signaling pathway have been identified,the detailed mechanisms are still *** previous results showed that OsMADS25 can regulate root development in response to nitrate signals,but the mechanism is still ***,we try to answer two key questions:how does OsMADS25 move from the cytoplasm to the nucleus,and what are the direct target genes activated by OsMADS25 to regulate root growth after it moves to the nucleus in response to nitrate?Our results demonstrated that OsMADS25 moves from the cytoplasm to the nucleus in the presence of nitrate in an *** immunoprecipitation sequencing,chromatin immunoprecipitation qPCR,yeast one-hybrid,and luciferase experiments showed that OsMADS25 directly activates the expression of OsMADS27 and OsARF7,which are reported to be associated with root ***,OsMADS25-RNAi lines,the Osnar2.1 mutant,and OsMADS25-RNAi Osnar2.1 lines exhibited significantly reduced root growth compared with the wild type in response to nitrate supply,and expression of OsMADS27 and OsARF7 was significantly suppressed in these ***,these results reveal a new mechanismby which OsMADS25 interacts with *** interaction is required for nuclear accumulation of OsMADS25,which promotes OsMADS27 and OsARF7 expression and root growth in a nitratedependent manner.

关键词： OsMADS25 OsNAR2.1 OsMADS27 OsARF7 nitrate signaling rice root

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生物柴油基恒流变钻井液体系

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石油勘探与开发 2022年第1期49卷 173-182页

作者：蒋官澄史赫贺垠博中国石油大学(北京)油气资源与探测国家重点实验室北京102249 中国石油大学(北京)石油工程教育部重点实验室北京102249 中国石油集团工程技术研究院有限公司北京102206

针对现阶段恒流变合成基钻井液基础油成本高昂且不可再生的缺陷,研究具有廉价、环保、可再生优势的生物柴油基恒流变钻井液技术。优选原材料制备成本低、环保且低温流动性好的大豆油乙酯生物柴油作为基础油,通过选择高油水比,并在辅乳... 详细信息

针对现阶段恒流变合成基钻井液基础油成本高昂且不可再生的缺陷,研究具有廉价、环保、可再生优势的生物柴油基恒流变钻井液技术。优选原材料制备成本低、环保且低温流动性好的大豆油乙酯生物柴油作为基础油,通过选择高油水比,并在辅乳化剂中引入阳离子表面活性剂,有效减缓了生物柴油基乳液因高温水解、皂化导致的增稠现象。使用由阳离子改性剂十六烷基三甲基氯化铵所制备的有机土可改善钻井液的流变性、稳定性和滤失造壁性,且不会引起低温稠化。使用二聚脂肪酸和椰子油脂肪酸二乙醇酰胺合成恒流变流型调节剂,可在生物柴油基中形成强网络结构并有效调控恒流变性。建立一套密度为1.2 g/cm^(3)的生物柴油基恒流变钻井液体系,恒流变温差范围达2~90℃,抗温达160℃,可抗5%海水、10%页岩屑污染,环保性能优良。

关键词：生物柴油恒流变钻井液乳化剂有机土流型调节剂环保深水钻井

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