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An Introduction to Product Specifications in China

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China Standardization 2010年第2期55卷 35-39页

作者： yin Li wang zheng

The government and enterprises in China started to pay attention to product specifications beginning with the launch of reform and, especially after the state promulgated the following two laws in 1993： the Product Quality Law （Article 15） and the Law on Protection of Consumer Rights and Interests （Articles 8, 13, 18, 22, 23, etc.）.

关键词：产品规格中国政府消费者权益保护法产品质量法

苦苣苔科大叶石上莲CYC类基因RT-PCR表达模式研究(英文)

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植物分类学报 2008年第1期46卷 23-31页

作者：杜志岩王印政系统与进化植物学国家重点实验室中国科学院植物研究所北京100093 中国科学院研究生院

CYC类基因的分子系统学研究已经在苦苣苔科Gesneriaceae中展开,但是还缺乏对这些基因表达和功能的研究。因此,我们选择苦苣苔科大叶石上莲Oreocharis benthamii作为实验材料,分离出了CYC类基因的两个拷贝,经过分子系统学分析这两个基因... 详细信息

CYC类基因的分子系统学研究已经在苦苣苔科Gesneriaceae中展开,但是还缺乏对这些基因表达和功能的研究。因此,我们选择苦苣苔科大叶石上莲Oreocharis benthamii作为实验材料,分离出了CYC类基因的两个拷贝,经过分子系统学分析这两个基因分别属于苦苣苔科GCYC1和GCYC2两个分支,故命名为ObCYC1和ObCYC2。分区的RT-PCR实验结果显示这两个基因拥有不同的时间空间表达模式。ObCYC1与模式植物金鱼草Antirrhinum majus中的CYC基因类似,集中在花冠背部区域表达,这与它们拥有保守的功能区TCP和R相一致。但是,ObCYC1与CYC表达模式仍有区别,即,和CYC相比ObCYC1在花冠背部区域的表达提前减弱。这可能和大叶石上莲花冠微弱的两侧对称性相关。另外,由于大叶石上莲的背部花瓣较两侧和腹部花瓣小,因此,在功能上ObCYC1可能起抑制背部花瓣生长作用而CYC基因则促进背部花瓣生长。与ObCYC1不同,ObCYC2的保守功能区有更多的氨基酸位点变化,而且在RT-PCR实验中也没有检测到它的表达。因此,需要开展更深入的实验研究分析ObCYC2的基本功能,这将有助于了解GCYC2类基因在苦苣苔科及其近缘科中的功能和进化途径。

关键词： CYCLOIDEA基因表达模式花对称性苦苣苔科大叶石上莲

CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin yin Yalan Xu Jie Mu Yu Leng Lei Ma Yu zheng Ruizhi Li yin wang Peifeng Li Hai Zhu Dong wang Jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

Significance of consensus CYC-binding sites found in the promoters of both ChCYC and ChRAD genes in Chirita heterotricha (Gesneriaceae)

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Journal of Systematics and Evolution 2010年第4期48卷 249-256页

作者： 1 Xia YANG 2Hong CUI 2Zu-Li YUAN 1yin-zheng wang1(State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China) 2(Henan Agricultural University,zhengzhou 450002,China) (State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Henan Agricultural University Zhengzhou 450002 China)

CYC-like genes are widely conserved in controlling floral dorsoventral asymmetry(zygomorphy)through persistent expression in corresponding domains in core *** understand how CYC-like gene expression is maintained during flower development,we selected Chirita heterotricha as a material and isolated the promoter sequences of the ChCYC1C and ChCYC1D genes,homologs of CYC,by inverse polymerase chain *** promoter analyses led to the identification of a putative cis-regulatory element in each promoter matching the consensus DNA binding site for Antirrhinum CYC protein:GGCCCCTC at-165 for ChCYC1C,and GGCCCCCC at-163 for *** indicates that both the ChCYC1C and ChCYC1D genes have probably evolved autoregulatory loops to sustain their expression in developing *** also isolated the coding and promoter sequences of the ChRAD gene,a homolog of Antirrhinum *** analysis showed that the ChRAD gene promoter also contained a putative CYC-binding site(GGCCCAC at-134).Therefore,ChRAD is likely a direct target of the ChCYC1 genes,which is similar to Antirrhinum *** results imply that the establishment of floral zygomorphy in Chirita may have been achieved by the evolution of an autoregulatory loop for CYC-like genes,which was probably accompanied by simultaneous co-option of the RAD-like gene into their regulatory network.

关键词： autoregulatory loop Chirita heterotricha CYC-binding sites CYCLOIDEA RADIALIS

Expression pattern of CYC-like genes relating to a dorsalized actinomorphic flower in Tengia(Gesneriaceae)

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Journal of Systematics and Evolution 2010年第5期48卷 309-317页

作者： 1Hong-Bo PANG 2Qing-Wen SUN 2Shun-Zhi HE 1yin-zheng wang 1(State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China) 2(Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China) (State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Guiyang College of Traditional Chinese Medicine Guiyang 550002 China)

Tengia has been called a "natural peloria" in the family Gesneriaceae because it exhibits an almost perfect actinomorphic flower from whorl one to whorl *** would be especially interesting to know whether or how CYC-like gene activities are related to this type of perfect actinomorphic *** address this,we have isolated four CYC-like TCP genes and carried out an investigation on their expression patterns in ***1C and TsCYC1D have similar expression patterns with strong signals being detected in all five petals and stamens,whereas TsCYC2A and TsCYC2B are only transiently expressed in the very early floral *** results suggest that the expansion of the expressions of TsCYC1C and TsCYC1D from the dorsal to the ventral petals is likely responsible for the evolutionary formation of the fully dorsalized actinomorphic corolla,that is,an expanded functional domain of CYC-like gene dorsal identity in Tengia ***,the expressions of TsCYC1C and TsCYC1D are not correlated with stamen abortion;therefore,TsCYC genes do not functionally repress the stamen development in Tengia *** is probably due to changed cis-activities that result in the cell cycle-related genes uncoupling from the TsCYC regulatory pathway in Tengia.

关键词： actinomorphy CYCLOIDEA dorsalized Gesneriaceae RNA in situ hybridization Tengia.

Clinical outcomes and immune reconstitution in 103 advanced AIDS patients undergoing 12-month highly active antiretroviral therapy

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Chinese Medical Journal 2006年第20期119卷 1677-1682页

作者： DAI Yi QIU Zhi-feng LI Tai-sheng HAN Yang ZUO Ling-yan XIE Jing MA Xiao-jun LIU zheng-yin wang Ai-xia Department of Infectious Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Background Highly active antiretroviral therapy （HAART） produces profound suppression of HIV replication, substantial increase in CD4^＋ T cells, and partial reconstitution of the immune system. However, the numbers of subjects were small in previous Chinese studies. This study evaluated the efficacy and side effects of HAART in Chinese advanced AIDS patients.Methods One hundred and three antiretroviral drug naive AIDS patients were enrolled in this study and were divided into two groups by their baseline CD4^＋ count：〈 100 cells/μl or ≥ 100 cells/μl. Clinical, virological and immunological outcomes were monitored at baseline and at 1, 3, 6, 9 and 12 months during the course of treatment with HAART.Results One patient died and another was lost from the follow-up. For the remaining 101 HIV/AIDS patients at the 12th month during the HAART, the plasma viral load （VL） was reduced to （3.2±0.7） lg copies/ml, the CD4^＋ count increased to （168 ±51） cells/μl [among which the naive phenotype （CD45RA^＋CD62L^＋） increased to （49 ±27） cells/μl and the memory phenotype （CD45RA^-） increased to （119 ±55） cells/μl], and the percentage of CD4^＋CD28^＋ cells increased. At the same time, there was a significant reduction of CD8^＋ T cell activation. In the 69 patients with the baseline CD4^＋ count 〈100 cells/μl, 37 had a VL 〈50 copies/ml; while in the 34 patients with the baseline CD4^＋ count ≥ 100 cells/μl, 25 had a VL 〈50 copies/ml, the difference between the two groups was statistically significant. The CD4^＋ T cell count showed a two-phase increase during HAART and a significant positive correlation was shown between the change of CD4^＋ count and plasma VL. Over 12 months of HAART, 10 patients had gastrointestinal side effects, 13 peripheral neuritis, 7 hepatic lesions, 8 hematological side effects, 8 skin rashes, 10 lipodystrophy and 1 renal calculus.Conclusions Immune reconstitution as well as the significantly improved clinical outcomes is observed in Chinese advanced AIDS pat

关键词： acquired immunodeficiency syndrome antiretroviral therapy, highly active CD4 lymphocyte count immune reconstitution adverse effects

Confirmation of a natural hybrid species in Petrocosmea(Gesneriaceae) based on molecular and morphological evidence

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Journal of Systematics and Evolution 2011年第5期49卷 449-463页

作者： 1,3~Zhi-Jing QIU 2~Zu-Li YUAN 1~Zhen-Yu LI 1~yin-zheng wang 1(State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China) 2(Henan Agricultural University,zhengzhou 45002,China) 3(Graduate School of the Chinese Academy of Sciences,Beijing 100049,China) (State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Graduate School of the Chinese Academy of Sciences Beijing 100049 China) (Henan Agricultural University Zhengzhou 45002 China)

A natural hybrid species in Petrocosmea named Longianthera in Yanshan County,Yunnan Province is confirmed for the first time based on molecular and morphological *** character count procedure of the variable characters show that Longianthera populations are characteristic of the intermediate morphological traits between its putative parents Yanshan and Petrocosmea *** nuclear ribosomal internal transcribed spacer region and three chloroplast regions of matK,trnL-F,and trnT-L are sequenced in the putative hybrid and the related *** alignment of DNA sequences and the phylogenetic trees could exclude all the other species in Petrocosmea as the parental species except for Yanshan and *** haplotypes in the 31 internal transcribed spacer sequences and six haplotypes in 42 cpDNA sequences were found from 14 individuals of Longianthera *** analyses of DNA sequences,haplotypes,and phylogenetic trees indicate that Longianthera is likely a hybrid species between its putative parents Yanshan and ***,in which Yanshan might be the most possible maternal *** factors may contribute to the natural hybridization between these two parental species in Petrocosmea,such as the overlapped geographic distribution,habitats,flowering periods,and shared ***,the new species of Yanshan and the natural hybrid species of Longianthera are described.

关键词： chloroplast DNA Gesneriaceae ITS morphology natural hybridization Petrocosmea.

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东庞矿综采工作面瓦斯综合治理

河北煤炭 2013年第1期 14-17页

作者：尹正旺冀中能源股份公司东庞矿河北邢台054201

随着东庞矿采掘活动向深部水平的延伸,矿井瓦斯涌出量日益增大,2011年矿井瓦斯等级鉴定结果为:绝对瓦斯涌出量39.15 m3/min,相对瓦斯涌出量5.87 m3/t,采面绝对瓦斯涌出量达到20 m3/min以上。瓦斯已严重威胁着矿井的安全生产和制约着综... 详细信息

随着东庞矿采掘活动向深部水平的延伸,矿井瓦斯涌出量日益增大,2011年矿井瓦斯等级鉴定结果为:绝对瓦斯涌出量39.15 m3/min,相对瓦斯涌出量5.87 m3/t,采面绝对瓦斯涌出量达到20 m3/min以上。瓦斯已严重威胁着矿井的安全生产和制约着综采工作面产能,为此东庞矿在综采工作面瓦斯治理工作上进行了深入的研究,制订了抽放系统设计及管理、通风系统设计及管理、瓦斯管理、安全监测监控设计及管理、防尘隔爆管理、防灭火设计及管理等瓦斯综合治理方案。此方案的实施,确保了综采工作面的安全、快速生产,为矿井的安全生产提供了保障。

关键词：瓦斯治理抽放系统通风系统管理

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半煤岩巷快速掘进支护参数优化研究

煤炭技术 2018年第8期37卷 12-14页

作者：郝长胜徐仁尹旭王政内蒙古科技大学矿业与煤炭学院内蒙古包头014010

针对纳二矿4-2煤层开拓大巷(半煤岩巷)过程中巷道支护返修率高、回采巷道多次发生冒顶事故、巷道掘进工程进度缓慢造成采掘接替紧张的问题,利用FLAC3D模拟软件对4-2煤层的支护参数进行优化研究,结果证明1 100 mm×1 000 mm的锚杆间排距... 详细信息

针对纳二矿4-2煤层开拓大巷(半煤岩巷)过程中巷道支护返修率高、回采巷道多次发生冒顶事故、巷道掘进工程进度缓慢造成采掘接替紧张的问题,利用FLAC3D模拟软件对4-2煤层的支护参数进行优化研究,结果证明1 100 mm×1 000 mm的锚杆间排距为最合理方案,在保证控制巷道围岩变形和维持正常使用的情况下,最具经济效益。

关键词：半煤岩巷快速掘进支护参数优化