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Inhibition of pancreatic stellate cell activity by adipose-derived stem cells

Hepatobiliary & Pancreatic Diseases International 2015年第2期14卷 215-221页

作者： Fu-Xiang Yu Long-Feng Su Chun-Lei Dai yang Wang yin-yan teng Jun-Hui Fu Qi-Yu Zhang yin-He Tang Department of Hepatobiliary and Pancreatic Surgery FirstAffiliatedHospital WenzhouMedicalCollege Department of Hepatobiliary and Pancreatic Surgery FirstAffiliatedHospitalWenzhouMedicalCollege

BACKGROUND：Pancreatic stellate cells（PSCs）play a critical role in the development of pancreatic *** this study we used a novel method to isolate and culture rat PSCs and then investigated the inhibitory effects of adipose-derived stem cells（ADSCs）on activation and proliferation of ***：Pancreatic tissue was obtained from SpragueDawley rats for PSCs *** cell cultures were adopted for co-culture of ADSCs and PSCs. PSCs prolifera- tion and apoptosis were determined using CCK-8 and flow cytometry, respectively, a-SMA expressions were analyzed using Western blotting. The levels of cytokines [nerve growth factor （NGF）, interleukin-10 （IL-10） and transforming growth factor-ill （TGF-[31）] in conditioned medium were detected by ELISA. Gene expression （MMP-2, MMP-9 and TIMP-1） was analyzed using qRT-PCR. RESULTS： This method produced 17.6_＋6.5 ~ 103 ceils per gram of the body weight with a purity of 90%-95% and a viability of 92%-97%. Co-culture of PSCs with ADSCs significantly inhib- ited PSCs proliferation and induced PSCs apoptosis. Moreover, a-SMA expression was significantly reduced in PSCs＋ADSCs compared with that in PSC-only cultures, while expression of fibrinolytic proteins （e.g., MMP-2 and MMP-9） was upregulated and anti-fibrinolytic protein （TIMP-1） was downregulated. In addition, NGF expression was up-regulated, but IL-10 and TGF-β1 expressions were down-regulated in the coculture conditioned medium compared with those in the PSC- only culture medium. CONCLUSIONS： This study provided an easy and reliable technique to isolate PSCs. The data demonstrated the inhibitory effects of ADSCs on the activation and proliferation of PSCs in vitro.

关键词： pancreatic stellate cells isolation mesenchymal stem cells pancreatic fibrosis fibrinolytic protein

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Neural Regeneration Research 2025年第2期20卷 366-377页

作者： Samuel Abokyi Dennis yan-yin Tse School of Optometry The Hong Kong Polytechnic UniversityKowloonHong Kong Special Administrative RegionChina Research Center for SHARP Vision The Hong Kong Polytechnic UniversityKowloonHong Kong Special Administrative RegionChina Center for Eye and Vision Research Sha TinHong Kong Special Administrative RegionChina

Retinal aging has been recognized as a significant risk factor for various retinal disorders,including diabetic retinopathy,age-related macular degeneration,and glaucoma,following a growing understanding of the molecular underpinnings of their *** comprehensive review explores the mechanisms of retinal aging and investigates potential neuroprotective approaches,focusing on the activation of transcription factor *** meta-analyses have demonstrated promising outcomes of transcription factor EB-targeted strategies,such as exercise,calorie restriction,rapamycin,and metformin,in patients and animal models of these common retinal *** review critically assesses the role of transcription factor EB in retinal biology during aging,its neuroprotective effects,and its therapeutic potential for retinal *** impact of transcription factor EB on retinal aging is cell-specific,influencing metabolic reprogramming and energy homeostasis in retinal neurons through the regulation of mitochondrial quality control and nutrient-sensing *** vascular endothelial cells,transcription factor EB controls important processes,including endothelial cell proliferation,endothelial tube formation,and nitric oxide levels,thereby influencing the inner blood-retinal barrier,angiogenesis,and retinal ***,transcription factor EB affects vascular smooth muscle cells,inhibiting vascular calcification and *** retinal pigment epithelial cells,transcription factor EB modulates functions such as autophagy,lysosomal dynamics,and clearance of the aging pigment lipofuscin,thereby promoting photoreceptor survival and regulating vascular endothelial growth factor A expression involved in *** cell-specific functions of transcription factor EB significantly impact retinal aging mechanisms encompassing proteostasis,neuronal synapse plasticity,energy metabolism,microvasculature,and inflammation,ultimately offering pro

关键词： age-related macular degeneration anti-aging interventions autophagy calorie restriction diabetic retinopathy exercise glaucoma neuromodulation phagocytosis photoreceptor outer segment degradation retinal aging transcription factor EB

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Neuronal regulated cell death in aging-related neurodegenerative diseases:key pathways and therapeutic potentials

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Neural Regeneration Research 2025年第8期20卷 2245-2263页

作者： Run Song Shiyi yin Jiannan Wu Junqiang yan Department of Neurology The First Affiliated HospitalCollege of Clinical Medicine of Henan University of Science and TechnologyLuoyangHenan ProvinceChina Neuromolecular Biology Laboratory The First Affiliated HospitalCollege of Clinical Medicine of Henan University of Science and TechnologyLuoyangHenan ProvinceChina

Regulated cell death(such as apoptosis,necroptosis,pyroptosis,autophagy,cuproptosis,ferroptosis,disulfidptosis)involves complex signaling pathways and molecular effectors,and has been proven to be an important regulatory mechanism for regulating neuronal aging and ***,excessive activation of regulated cell death may lead to the progression of aging-related *** review summarizes recent advances in the understanding of seven forms of regulated cell death in age-related ***,the newly identified ferroptosis and cuproptosis have been implicated in the risk of cognitive impairment and neurodegenerative *** forms of cell death exacerbate disease progression by promoting inflammation,oxidative stress,and pathological protein *** review also provides an overview of key signaling pathways and crosstalk mechanisms among these regulated cell death forms,with a focus on ferroptosis,cuproptosis,and *** instance,FDX1 directly induces cuproptosis by regulating copper ion valency and dihydrolipoamide S-acetyltransferase aggregation,while copper mediates glutathione peroxidase 4 degradation,enhancing ferroptosis ***,inhibiting the Xc-transport system to prevent ferroptosis can increase disulfide formation and shift the NADP^(+)/NADPH ratio,transitioning ferroptosis to *** insights help to uncover the potential connections among these novel regulated cell death forms and differentiate them from traditional regulated cell death *** conclusion,identifying key targets and their crosstalk points among various regulated cell death pathways may aid in developing specific biomarkers to reverse the aging clock and treat age-related neurodegenerative conditions.

关键词： apoptosis autophagy cuproptosis disulfidptosis ferroptosis necroptosis neurodegenerative disease neurological aging diseases PANoptosis pyroptosis

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Non-coding RNAs in acute ischemic stroke:from brain to periphery

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Neural Regeneration Research 2025年第1期20卷 116-129页

作者： Shuo Li Zhaohan Xu Shiyao Zhang Huiling Sun Xiaodan Qin Lin Zhu teng Jiang Junshan Zhou Fuling yan Qiwen Deng Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Zhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsu ProvinceChina General Clinical Research Center Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina

Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and *** predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain *** innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental *** expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic ***,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and *** particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic *** this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and *** from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.

关键词： acute ischemic stroke apoptosis blood–brain barrier damage circular RNAs excitatory toxicity long non-coding RNAs microRNAs neuroinflammation non-coding RNAs oxidative stress

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基于社交用户标签的混合top-N推荐方法

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计算机应用研究 2013年第5期30卷 1309-1311,1344页

作者：蔡孟松李学明尹衍腾重庆大学计算机学院重庆400044

针对协同过滤方法的冷启动问题,提出一种将社交用户标签与协同过滤相结合的混合top-N推荐方法。通过社交用户关系获得可信用户集,然后根据个性化标签采用结构上下文相似性算法(SimRank)计算社交用户相似近邻集并进行预测推荐,最后结合... 详细信息

针对协同过滤方法的冷启动问题,提出一种将社交用户标签与协同过滤相结合的混合top-N推荐方法。通过社交用户关系获得可信用户集,然后根据个性化标签采用结构上下文相似性算法(SimRank)计算社交用户相似近邻集并进行预测推荐,最后结合传统协同过滤方法进行推荐。实验结果表明,该方法能够提高在一般数据集及冷启动用户数据集下的推荐精度。

关键词：推荐系统协同过滤社交网络个性化标签冷启动

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Nanomaterials-mediated lysosomal regulation:a robust protein-clearance approach for the treatment of Alzheimer’s disease

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Neural Regeneration Research 2025年第2期20卷 424-439页

作者： Mengqi Hao Jianjian Chu Tinglin Zhang Tong yin Yuankai Gu Wendanqi Liang Wenbo Ji Jianhua Zhuang yan Liu Jie Gao You yin Department of Neurology Second Affiliated Hospital of Naval Medical University(Shanghai Changzheng Hospital)ShanghaiChina School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghaiChina Changhai Clinical Research Unit Shanghai Changhai HospitalNaval Medical UniversityShanghaiChina Department of Clinical Pharmacy Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Department of Neurology Shanghai East HospitalSchool of MedicineTongji UniversityShanghaiChina

Alzheimer’s disease is a debilitating,progressive neurodegenerative disorder characterized by the progressive accumulation of abnormal proteins,including amyloid plaques and intracellular tau tangles,primarily within the ***,crucial intracellular organelles responsible for protein degradation,play a key role in maintaining cellular *** studies have suggested a link between the dysregulation of the lysosomal system and pathogenesis of neurodegenerative diseases,including Alzheimer’s *** the normal physiological function of lysosomes hold the potential to reduce the pathological burden and improve the symptoms of Alzheimer’s ***,the efficacy of drugs in treating Alzheimer’s disease is limited,with major challenges in drug delivery efficiency and ***,nanomaterials have gained widespread use in Alzheimer’s disease drug research owing to their favorable physical and chemical *** review aims to provide a comprehensive overview of recent advances in using nanomaterials(polymeric nanomaterials,nanoemulsions,and carbon-based nanomaterials)to enhance lysosomal function in treating Alzheimer’s *** review also explores new concepts and potential therapeutic strategies for Alzheimer’s disease through the integration of nanomaterials and modulation of lysosomal *** conclusion,this review emphasizes the potential of nanomaterials in modulating lysosomal function to improve the pathological features of Alzheimer’s *** application of nanotechnology to the development of Alzheimer’s disease drugs brings new ideas and approaches for future treatment of this disease.

关键词： Alzheimer’s disease autophagy dysfunction lysosomal acidification lysosomal system nanomaterials neurodegenerative diseases

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基于用户关系与属性的微博意见领袖挖掘方法

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计算机工程 2013年第4期39卷 184-189页

作者：尹衍腾李学明蔡孟松重庆大学计算机学院重庆400044

提出一种结合用户关系与用户属性的挖掘方法。根据微博特征构建微博用户关系网,采用小世界网络理论确定用户的中心性,以此获得基于用户关系的候选意见领袖。通过分析微博用户属性,建立意见领袖影响体系,提出D-means聚类算法,获得基于用... 详细信息

提出一种结合用户关系与用户属性的挖掘方法。根据微博特征构建微博用户关系网,采用小世界网络理论确定用户的中心性,以此获得基于用户关系的候选意见领袖。通过分析微博用户属性,建立意见领袖影响体系,提出D-means聚类算法,获得基于用户属性的候选意见领袖,结合2种候选意见领袖得到最终意见领袖。实验结果验证该方法在挖掘意见领袖上比现有方法更加准确有效。

关键词：意见领袖微博用户关系用户属性小世界网络聚类分析

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Human-induced pluripotent stem cell-derived neural stem cell exosomes improve blood-brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis

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Neural Regeneration Research 2025年第2期20卷 518-532页

作者： Conglin Wang Fangyuan Cheng Zhaoli Han Bo yan Pan Liao Zhenyu yin Xintong Ge Dai Li Rongrong Zhong Qiang Liu Fanglian Chen Ping Lei Department of Geriatrics Tianjin Medical University General HospitalTianjinChina School of Medicine Nankai UniversityTianjinChina Tianjin Neurological Institute TianjinChina

Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor ***-induced pluripotent stem cell-derived neural stem cell exosomes(hiPSC-NSC-Exos)have shown potential for brain injury repair in central nervous system *** this study,we explored the impact of hiPSC-NSC-Exos on blood-brain barrier preservation and the underlying *** results indicated that intranasal delivery of hiPSC-NSC-Exos mitigated neurological deficits,enhanced blood-brain barrier integrity,and reduced leukocyte infiltration in a mouse model of intracerebral ***,hiPSC-NSC-Exos decreased immune cell infiltration,activated astrocytes,and decreased the secretion of inflammatory cytokines like monocyte chemoattractant protein-1,macrophage inflammatory protein-1α,and tumor necrosis factor-αpost-intracerebral hemorrhage,thereby improving the inflammatory *** sequencing indicated that hiPSC-NSC-Exo activated the PI3K/AKT signaling pathway in astrocytes and decreased monocyte chemoattractant protein-1 secretion,thereby improving blood-brain barrier *** with the PI3K/AKT inhibitor LY294002 or the monocyte chemoattractant protein-1 neutralizing agent C1142 abolished these *** summary,our findings suggest that hiPSC-NSC-Exos maintains blood-brain barrier integrity,in part by downregulating monocyte chemoattractant protein-1 secretion through activation of the PI3K/AKT signaling pathway in astrocytes.

关键词： AKT astrocyte blood-brain barrier cerebral edema exosomes human-induced pluripotent stem cells intracerebral hemorrhage neural stem cells neuroinflammation PI3K

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Magneto-electric behaviors of La_(0.67-x)Nd_(x)Sr_(0.33)MnO_(3) system

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Rare Metals 2022年第4期41卷 1357-1363页

作者： Ning Liu teng Guo Guo-Qing yan Guang Zhu Huan-yin Guo College of Machinery and Automobile Engineering Anhui Polytechnic UniversityWuhu 241000China Anhui Key Laboratory of Spin Electron and Nanomaterials(Cultivating Base) Suzhou CollegeSuzhou 234000China College of Chemistry and Materials Engineering Anhui Science and Technology UniversityFengyang 233100China National Laboratory of Solid State Microstructures Nanjing UniversityNanjing 210093China

The polycrystalline samples La_(0.67-x)Nd_(x)Sr_(0.33)MnO_(3)(x=0.1,0.2,0.3,0.4,0.5)were prepared by standard solid-state reaction method,and the M–T curves,electron spin resonance(ESR)curves,infrared spectra,Raman spectra,ρ–T curves and MR–T curves of the compounds above were *** results show that single phase can be obtained at different Nd substitution ratios,and with the increase in Nd doping,the magnetic structure of the system transforms from long-range ferro-magnetic order to spin cluster glass state and antiferro-magnetic state;and the phase separation appears in the samples with x=0.3 and *** transport property exhibits variation with the increase in Nd doping,and the electric behavior of the sample transforms(from metal state to insulator–metal transition,and then insulator state)is accompanied by colossal magnetoresistance(CMR)effect,which can be attributed to the different couplings of sublattices induced by Nd doping and the interface tun-neling effect related to the spin.

关键词： Magnetic structure Transport behavior Sublattice Interface tunneling effect

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IDV:Internet Domain Name Verification Based on Blockchain

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Computer Modeling in Engineering & Sciences 2021年第10期129卷 299-322页

作者： Ning Hu Yu teng yan Zhao Shi yin Yue Zhao Cyberspace Institute of Advanced Technology Guangzhou UniversityGuangzhou510006China Zhong Zi Hua Ke Traffic Construction Technology Co. Ltd.Beijing100195China Science and Technology on Communication Security Laboratory Chengdu610041China

The rapid development of blockchain technology has provided new ideas for network security ***-based network security enhancement solutions are attracting widespread *** paper proposes an Internet domain name verification method based on *** authenticity of DNS(Domain Name System)resolution results is crucial for ensuring the accessibility of Internet *** to the lack of adequate security mechanisms,it has always been a challenge to verify the authenticity of Internet domain name resolution *** the solution represented by DNSSEC(Domain Name System Security Extensions)can theoretically solve the domain name verification problem,it has not been widely deployed on a global scale due to political,economic,and technical *** argue that the root cause of this problem lies in the significant centralization of the DNS *** centralized feature not only reduces the efficiency of domain name verification but also has the hidden risks of single point of failure and unilateral *** users may disappear from the Internet due to the results of fake,subverted,or misconfigured domain name *** paper presents a decentralized DNS cache verification method,which uses the consortium blockchain to replace the root domain name server to verify the authenticity of the domain *** with DNSSEC’s domain name verification process,the verification efficiency of this method has increased by 30%,and there is no single point of failure or unilateral control *** addition,this solution is incrementally deployable,and even if it is deployed on a small number of content delivery network servers,satisfactory results can be obtained.

关键词： Blockchain-based network security DNS security DNS decentralization CDN

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