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Chinese Journal of Chemical Engineering 2024年第1期65卷 276-285页

作者： yin wu Yan Sun Department of Biochemical Engineering School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology(Ministry of Education)Tianjin UniversityTianjin 300350China

Biocatalysis in organic solvents(OSs)has numerous important applications,but native enzymes in OSs often exhibit limited catalytic ***,we proposed a computation-aided surface charge engineering strategy to improve the catalytic performance of haloalkane dehalogenase DhaA in OSs based on the energetic analysis of substrate binding to the DhaA *** variants with enhanced OS resistance were obtained by replacing negative charged residues on the surface with positive charged residue(Arg).Particularly,a four-substitution variant E16R/E93R/E121R/E257R exhibited the best catalytic performance(five-fold improvement in OS resistance and seven-fold half-life increase in 40%(vol)dimethylsulfoxide).As a result,the overall catalytic performance of the variant could be at least 26 times higher than the wild-type *** spectroscopy and molecular dynamics simulation studies revealed that the residue substitution mainly enhanced OS resistance from four aspects:(a)improved the overall structural stability,(b)increased the hydrophobicity of the local microenvironment around the catalytic triad,(c)enriched the hydrophobic substrate around the enzyme molecule,and(d)lowered the contact frequency between OS molecules and the catalytic *** findings validate that computationaided surface charge engineering is an effective and ingenious rational strategy for tailoring enzyme performance in OSs.

关键词： Surface charge engineering Organic solvent resistance Molecular dynamics simulation Haloalkane dehalogenase

LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus

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Zoological Research 2024年第1期45卷 25-35页

作者： Xian-Hui Ning Bing Han Ye Peng Shao-wu yin College of Marine Science and Engineering Nanjing Normal UniversityNanjingJiangsu 210023China CAS Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdaoShandong 266071China Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province LianyungangJiangsu 222005China

Long non-coding RNAs(lncRNAs)function as key modulators in mammalian immunity,particularly due to their involvement in lncRNA-mediated competitive endogenous RNA(ceRNA)*** their recognized significance in mammals,research on lncRNAs in lower vertebrates remains *** the present study,we characterized the first immune-related lncRNA(pol-lnc78)in the teleost Japanese flounder(Paralichthys olivaceus).Results indicated that pol-lnc78 acted as a ceRNA for pol-miR-n199-3p to target the sterile alpha and armadillo motif-containing protein(SARM),the fifth discovered member of the Toll/interleukin 1(IL-1)receptor(TIR)adaptor *** ceRNA network regulated the antibacterial responses of flounder via the Toll-like receptor(TLR)signaling ***,SARM acted as a negative regulator and exacerbated bacterial infection by inhibiting the expression of inflammatory cytokines IL-1βand tumor necrosis factor-α(TNF-α).Pol-miR-n199-3p reduced SARM expression by specifically interacting with the 3’untranslated region(UTR),thereby promoting SARM-dependent inflammatory cytokine expression and protecting the host against bacterial ***,pol-lnc78 sponged pol-miR-n199-3p to ameliorate the inhibition of SARM *** infection,the negative regulators pol-lnc78 and SARM were significantly down-regulated,while pol-miR-n199-3p was significantly up-regulated,thus favoring host antibacterial *** findings provide novel insights into the mechanisms underlying fish immunity and open new horizons to better understand ceRNA crosstalk in lower vertebrates.

关键词： LncRNA SARM miRNA ceRNA Antibacterial response

Development and validation of a nomogram model for predicting the risk of gallstone recurrence after gallbladder-preserving surgery

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Hepatobiliary & Pancreatic Diseases International 2024年第3期23卷 288-292页

作者： Peng Liu Yong-Wei Chen Che Liu yin-Tao wu Wen-Chao Zhao Jian-Yong Zhu Yang An Nian-Xin Xia Faculty of Hepato-Pancreato-Biliary Surgery the First Medical CenterChinese PLA General HospitalBeijing 100853China Institute of Hepatobiliary Surgery of Chinese PLA Beijing 100853China Key Laboratory of Digital Hepetobiliary Surgery of Chinese PLA Beijing 100853China

Background:The high incidence of gallstone recurrence was a major concern for laparoscopic gallbladderpreserving *** study aimed to investigate the risk factors for gallstone recurrence after gallbladder-preserving surgery and to establish an individualized nomogram model to predict the risk of gallstone ***:The clinicopathological and follow-up data of 183 patients who were initially diagnosed with gallstones and treated with gallbladder-preserving surgery at our hospital from January 2012 to January 2019 were retrospectively *** independent predictive factors for gallstone recurrence following gallbladder-preserving surgery were identified by multivariate logistic regression analysis.A nomogram model for the prediction of gallstone recurrence was constructed based on the selected *** C-index,receiver operating characteristic(ROC)curve and calibration curve were used to evaluate the predictive power of the nomogram model for gallstone ***:During the follow-up period,a total of 65 patients experienced gallstone recurrence,and the recurrence rate was 35.5%.Multivariate logistic regression analysis revealed that the course of gallstones>2 years[odds ratio(OR)=2.567,95%confidence interval(CI):1.270-5.187,P=0.009],symptomatic gallstones(OR=2.589,95%CI:1.059-6.329,P=0.037),multiple gallstones(OR=2.436,95%CI:1.133-5.237,P=0.023),history of acute cholecystitis(OR=2.778,95%CI:1.178-6.549,P=0.020)and a greasy diet(OR=2.319,95%CI:1.186-4.535,P=0.014)were independent risk factors for gallstone recurrence after gallbladder-preserving surgery.A nomogram model for predicting the recurrence of gallstones was established based on the above five *** results showed that the C-index of the nomogram model was 0.692,suggesting it was valuable to predict gallstone ***,the calibration curve showed good consistency between the predicted probability and actual ***:The nomogram model for

关键词： Gallstone Gallbladder-preserving surgery Recurrence Risk factors Nomogram

Incidence and warning signs for complications of human brucellosis: a multi-center observational study from China

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Infectious Diseases of Poverty 2024年第1期13卷 53-62页

作者： Qing-Nan Shi Hui-Jie Qin Qiao-Shan Lu hu Li Zhong-Fa Tao Meng-Guang Fan Mu-Heta Aishan Zeng-Qiang Kou Qiu-Lan Chen Wen-wu yin Yan-Ping Zhang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and PreventionChangbai RoadChangping DistrictBejing 102206China School of Public Health Guangxi Medical UniversityNanningChina Nanning Center for Disease Control and Prevention NanningChina Guizhou Center for Disease Control and Prevention GuiyangChina Inner Mongolia Center for Disease Control and Prevention HohhotChina Xinjiang Uighur Autonomous Region Center for Disease Control and Prevention UrumqiChina Shandong Center for Disease Control and Prevention JinanChina

Background Brucellosis is a severe zoonotic disease that is often overlooked, particularly in impoverished countries. Timely identification of focal complications in brucellosis is crucial for improving treatment outcomes. However, there is currently a lack of established indicators or biomarkers for diagnosing these complications. Therefore, this study aimed to investigate potential warning signs of focal complications in human brucellosis, with the goal of providing practical parameters for clinicians to aid in the diagnosis and management of *** A multi-center cross-sectional study was conducted in China from December 2019 to August 2021. The study aimed to investigate the clinical characteristics and complications of patients with brucellosis using a questionnaire survey and medical record system. The presence of warning signs for complications was assessed using univariate and multivariate logistic regression models. Receiver operating characteristic (ROC) curves and the area under the curve (AUC) were used for variable screening and model *** A total of 880 participants diagnosed with human brucellosis were enrolled. The median age of the patients was 50 years [interquartile range (IQR): 41.5-58.0], and 54.8% had complications. The most common organ system affected by complications was the osteoarticular system (43.1%), with peripheral arthritis (30.0%), spondylitis (16.6%), paravertebral abscess (5.0%), and sacroiliitis (2.7%) being the most prevalent. Complications in other organ systems included the genitourinary system (4.7%), respiratory system (4.7%), and hematologic system (4.6%). Several factors were found to be associated with focal brucellosis. These factors included a long delay in diagnosis [odds ratio (OR) = 3.963, 95% confidence interval(CI): 1.906-8.238 for > 90 days], the presence of underlying disease (OR = 1.675, 95%CI: 1.176-2.384), arthralgia (OR = 3.197, 95%CI: 1.986-5.148), eye bulging pain (OR = 3.482, 95%CI:

关键词： Brucellosis Complications Clinical spectrum Warning signs

基于CTGAN与GDMPA-RF算法的活立木含水率诊断方法优化研究

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传感技术学报 2024年第6期37卷 1025-1034页

作者：杨能飞吴寅南京林业大学信息科学技术学院江苏南京210037

活立木含水率的精准实时诊断是智慧林业领域的重要研究内容,其可为植物生理状态分析、林区生态水文调控、林火预警防范等做出关键指征。基于无线声发射传感器网络(Wireless Acoustic Sensor Network,WASN)系统的含水率测定方法既可实现... 详细信息

活立木含水率的精准实时诊断是智慧林业领域的重要研究内容,其可为植物生理状态分析、林区生态水文调控、林火预警防范等做出关键指征。基于无线声发射传感器网络(Wireless Acoustic Sensor Network,WASN)系统的含水率测定方法既可实现高效无损探测,又能长期野外部署,尤为适合林场实际需求。为了进一步提升WASN的辨识准确率,首先利用条件表格生成对抗网络(Conditional Tabular GAN,CTGAN)对所采集的AE特征进行数据增广,其次基于分布式梯度提升框架(Light Gradient Boosting Machine,LightGBM)对扩增后的混合数据集进行特征优选,然后提出了黄金正弦动态海洋捕食者算法优化的随机森林(Golden-Sine Dynamic Marine Predators Algorithm-Random Forests,GDMPA-RF)策略,并以此建立含水率精准反演模型。实验对比结果显示,基于优选特征子集构建的GDMPA-RF模型在立木含水率诊断性能强化方面效果最佳,其准确率(Accuracy)、精确率(Precision)、F1分数(F1-Score)、加权平均(Weighted Average)和AUC分别为99.17%、99.52%、98.14%、0.9943和0.9850,均高于鲸鱼优化算法等结合RF模型的评估指标,说明方法具有优良的监测效能,较好地优化了活立木树干含水率的在线实时推演精度。

关键词：无线声发射传感器网络活立木含水率条件表格生成对抗网络黄金正弦动态海洋捕食者算法随机森林

荧光原位杂交扩增探针的结构光照明超分辨成像与计数

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激光与光电子学进展 2024年第4期61卷 183-188页

作者：吴寅梁永张洁李辉中国科学技术大学生物医学工程学院(苏州) 生命科学与医学部江苏苏州215163 中国科学院苏州生物医学工程技术研究所江苏省医用光学重点实验室江苏苏州215163

人类表皮生长因子受体-2(HER2)的异常扩增会导致癌细胞的过度增殖和肿瘤恶化。在采用常规光学显微成像技术检测扩增水平较高的乳腺癌细胞HER2基因时,荧光原位杂交探针的荧光信号斑点呈簇状分布,难以精确计数。应用结构光照明超分辨成像... 详细信息

人类表皮生长因子受体-2(HER2)的异常扩增会导致癌细胞的过度增殖和肿瘤恶化。在采用常规光学显微成像技术检测扩增水平较高的乳腺癌细胞HER2基因时,荧光原位杂交探针的荧光信号斑点呈簇状分布,难以精确计数。应用结构光照明超分辨成像技术对HER2基因荧光原位杂交的病理切片进行成像,从而分辨距离较近的荧光探针。通过大视场扫描成像和图像拼接,对数百个细胞进行成像和统计分析,提高了高扩增水平病理切片上HER2探针计数的准确性。

关键词：乳腺癌病理诊断荧光原位杂交结构光照明超分辨成像图像拼接

跨行政区域公路交通的一体化对区域经济发展的影响

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公路 2024年第4期69卷 208-215页

作者：吴寅恺王虹安徽省社会科学院区域现代化研究院合肥市230053

跨行政区域公路交通的一体化是推进区域一体化发展的基础和先导。基于对长三角省际毗邻县域地区公路交通建设情况的分析,构建多期DID模型,研究了跨行政区域公路交通一体化对省际毗邻地区经济发展的影响效应。结果显示,省际毗邻县域地区... 详细信息

跨行政区域公路交通的一体化是推进区域一体化发展的基础和先导。基于对长三角省际毗邻县域地区公路交通建设情况的分析,构建多期DID模型,研究了跨行政区域公路交通一体化对省际毗邻地区经济发展的影响效应。结果显示,省际毗邻县域地区公路交通一体化为地区经济发展带来了“水平效应”,却没有带来“增长效应”。同时,公路交通一体化会促进省际毗邻地区间人口的流动,但在短期内却无法优化地区的产业结构。此外,相较于发达地区,公路交通一体化能够通过“扩散效应”让发达地区带动欠发达地区经济的发展。为此,建议要进一步加大省际毗邻地区公路基础设施的路网密度,以公路交通一体化带动欠发达地区的经济发展,以实现省际毗邻地区间的协调发展。

关键词：公路交通一体化省际毗邻地区经济发展多期DID模型

以金融科技创新和高质量发展全面推进金融强国建设

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中国井冈山干部学院学报 2024年第3期17卷 81-90页

作者：吴垠西南财经大学经济学院四川成都611130

金融是现代经济的核心,是国家发展的重要核心竞争力,是促进经济高质量发展的重要一环。在新的历史阶段,系统性纵深推进科技创新,使其与金融领域结合并全面深化拓展,更好地赋能金融科技,是我们深入实施创新驱动发展战略、规避金融领域各... 详细信息

金融是现代经济的核心,是国家发展的重要核心竞争力,是促进经济高质量发展的重要一环。在新的历史阶段,系统性纵深推进科技创新,使其与金融领域结合并全面深化拓展,更好地赋能金融科技,是我们深入实施创新驱动发展战略、规避金融领域各种风险、建设现代化金融科技强国的必由之路。立足于新发展阶段,我们要坚持以人民为中心的发展思想,坚定不移地贯彻新发展理念,全方位落实金融科技创新对高质量发展的积极作用。尤其是面对中国式现代化发展的战略机遇、战略挑战和目标要求,需要立足于新发展阶段在金融领域实现新的突破,更好地实现金融高质量发展,以金融赋能全面推进金融强国建设。

关键词：金融科技创新金融高质量发展中国式现代化金融强国

基于倾向评分匹配法的甲状腺结节患者日间手术实施效果评价

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中国普通外科杂志 2024年第5期33卷 856-860页

作者：刘雍容吴垠刘敏韩赛中南大学湘雅三医院手术中心湖南长沙410013

背景与目的:随着医疗技术的进步和患者需求的提升,日间手术逐渐成为甲状腺结节治疗的重要选择。因此,本研究评价甲状腺结节患者日间手术的实施效果,为临床提供实践参考。方法:本研究采用回顾性研究方法,选择中南大学湘雅三医院2022年1月... 详细信息

背景与目的:随着医疗技术的进步和患者需求的提升,日间手术逐渐成为甲状腺结节治疗的重要选择。因此,本研究评价甲状腺结节患者日间手术的实施效果,为临床提供实践参考。方法:本研究采用回顾性研究方法,选择中南大学湘雅三医院2022年1月1日—2023年12月31日946例行甲状腺结节日间手术和非日间手术患者为研究对象,将实施日间手术模式的患者作为日间手术组,实施住院手术模式的患者作为非日间手术组。采用倾向评分匹配法,为日间手术组在非日间手术组中以1∶1匹配到最接近的样本,确保两组基本资料(性别、年龄、麻醉分级、手术级别、TI-RADS分级、结节直径、结节涉及范围、病理类型)均衡可比,然后对匹配后的样本进行效果评价。结果:匹配前946例患者中,日间手术组102例,非日间手术组844例,两组的年龄、结节直径、TI-RADS分级差异有统计学意义(均P0.05)。匹配后的数据显示,两组患者均无严重并发症发生;日间手术组在手术时间、出血量、术后伤口引流量、伤口引流管留置时间、术后疼痛评分方面均优于非日间手术组(均P<0.05);与非日间手术组比较,日间手术组总住院费用(18657.21元vs.24664.35元)、检查费用占比(14.50%vs.19.34%)、药品费用占比(10.42%vs.12.69%)及住院时间(2 d vs.4 d)均明显降低,但劳务价值占比(31.14%vs.28.53%)和耗材费用占比(31.2%vs.26.87%)更高(均P<0.05)。结论:对于符合日间手术准入标准的甲状腺结节患者,日间手术安全性好,可有效降低甲状腺结节患者住院费用,缩短平均住院时间,可有效提高医疗资源利用率和患者满意度。

关键词：甲状腺结节门诊外科手术倾向性评分

数字技术赋能公共体育服务发展效应的实证检验

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上海体育大学学报 2024年第6期48卷 81-93,104页

作者：张叶涵陈嘉良李海吴殷上海体育大学经济管理学院上海200438

数字技术是新质生产力赋能公共体育服务发展的关键力量和重要体现。基于2014—2020年中国30个省(区、市)(不含香港地区、澳门地区、台湾地区、西藏)的面板数据,运用双向固定效应模型、动态门槛回归模型,实证分析数字技术、政府治理能力... 详细信息

数字技术是新质生产力赋能公共体育服务发展的关键力量和重要体现。基于2014—2020年中国30个省(区、市)(不含香港地区、澳门地区、台湾地区、西藏)的面板数据,运用双向固定效应模型、动态门槛回归模型,实证分析数字技术、政府治理能力和公共体育服务发展三者间的关系。发现:(1)在直接效应上,数字技术对公共体育服务发展具有显著的正向促进作用,且考虑内生变量和稳健性检验后结论依然成立;(2)在门槛效应上,随着数字技术的进步,数字技术对公共体育服务发展呈现边际效应递减的显著正向影响;(3)在区域异质性上,相较于其他地区,研究时间、区间内数字技术对西部地区公共体育服务发展的影响效应更明显;(4)在中介效应上,提升政府治理能力是数字技术对公共体育服务发展影响效应的重要作用机制。基于此,对未来中国不同区域如何更好地促进数字技术与公共体育服务深度融合、推动公共体育服务的高质量发展提出相关政策建议。

关键词：数字技术公共体育服务发展政府治理能力双向固定效应模型动态门槛回归模型中介效应

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