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Nemania bipapillata was firstly identified from tea leaf spot disease in Yuqing,Guizhou Province,China

Nemania bipapillata was firstly identified from tea leaf spo...

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中国植物病理学会2019年学术年会

作者： Wang Xue yin qiaoxiu Li Dongxue Ren Yafeng Wu Xian Jiang Shilong Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Tea plant(Camellia sinensis) is a major crop in Guizhou province,*** the end of 2017,the cultivated area of tea plant covered 350 000 *** November 2018,tea leaf spot was caused a huge loss of the production of tea in Yuqing county in Guizhou *** symptoms of the infected tea leaves were brown,irregular and the center of lesion appeared to be *** some tea gardens,disease incidence of leaves was 46% to 52%,and decreased seriously the production of tea *** this study,some fungi from tea disease samples were identified,such as Phomopsis foeniculi,Diaporthe ganias,et ***,some isolates were obtained to our *** colony of GZYQ-03-02 on PDA initially was white,and then the colony was gradually changed to black after 14 days *** genes of DNA-dependent RNA polymerase II second largest subunit(RPB2),beta-tubulin 2(β-TUB2) and actin(ACT) were amplified and sequenced from the isolates,and then deposited in Genbank(Accession ***852276,MK852273 and MK852274 for the isolate GZYQ-03-02).Maximum parsimony phylogenetic analysis based on concatenated sequences of combined ACT(1-312),β-TUB2(313-506),RPB2(507-847) indicated that the isolate GZYQ-03-02 was identical to reference strains Nemania bipapillata 90080610,and the clade was supported by 99% bootstrap *** fulfill Koch’s postulates,unwounded and wounded tea leaves were inoculated with mycelial plugs on PDA,and non-symptoms were observed after 7 days of *** was known as synonym of Nemania *** caused tea stem wood decay in Sri ***,*** was also known as endophyte,which was isolated from plant hosts of Lycium chinense,Cephalotaxus,Vanilla planifolia,et *** our knowledge,we firstly isolated the fungus from tea *** mechanism of the fungus in the tea leaf spot is worth studying in the future.

关键词： Nemania bipapillata identification pathogenicity test leaf spot pathogenicity mechanism

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Pithomyces chartarum was firstly identified from tea leaf spot in Yuqing,Guizhou Province

Pithomyces chartarum was firstly identified from tea leaf sp...

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中国植物病理学会2019年学术年会

作者： yin qiaoxiu Wang Xue Ren Yafeng Li Dongxue Jiang Shilong Wu Xian Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Leaf spot disease is an important disease of tea(Camellia sinensis) in Yuqing county,Guizhou *** symptoms were small,round or irregular *** lesion gradually enlarged to display the darker lesion margin with a bright yellowish-black *** center of lesion appeared to be *** adjacent lesions have been merged to the larger lesion and covered the margin of the *** disease takes place in tea garden during autumn on altitude from one thousand to one thousand three hundred *** incidence of leaves was estimated at 74% to 82%,depending on the *** severity on a plant basis was estimated to be 68% to 76%.In order to identify the pathogens of this disease,we isolated and identified some fungi from tea samples with the symptoms of leaf spot,and conducted the pathogenicity *** found Phoma segeticola,Didymella bellidis and Pestalotiopsis trachicarpicola cause the lesions of tea leaves by Koch’s *** addition,we also isolated and identifed the isolate of GZYQ4-1-d from the samples of tea *** colony of GZYQ4-1-d on PDA was regular and round,flat growing,sometimes floccose,*** time,the colony became grey to dark grey because the pigment of mycelium *** are typically pigmented,muriform,sometimes echinulate,usually with 3 transverse septa and with 0-2 longitudinal septa,measured to(17.16-28.38) μm×(12.57-18.04) μ*** internal transcribed spacer(ITS) regions 1 and 2 was used to amplify and sequenced,and the sequence revealed 100% identity with *** by Blastn *** pathogenicity test was conducted result indicated that the strains can induce leaf spot on leaves of *** leaves of the tea plant varieties of Moss tea and Fuding Dabaicha were inoculated with mycelial plugs on PDA or the spore suspension(1×10 spores per mL) using the methods of unwound,puncture or *** symptom has not been observed on inoculated leaves for three ***,some literatures reported t

关键词： Pithomyces chartarum identification pathogenicity test leaf spot pathogenicity mechanism

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The transcriptome and ultrastructure uncover the anti-fungal mechanism of Ningnanmycin against Pseudopestalotiopsis camelliae-sinensis

The transcriptome and ultrastructure uncover the anti-fungal...

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中国植物病理学会2019年学术年会

作者： Wang Xue Wen Xiaodong Song Xingchen yin qiaoxiu Li Dongxue Ren Yafeng Wu Xian Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Tea grey blight is an important disease of tea plant(Camellia sinensis) all over the world,which leads to a huge loss of tea *** camelliae-sinensis is the causal agent of tea grey blight in tea garden in Guizhou Province,***(NNM),which was known as an environmentally friendly microbial pesticide,can effectively prevent and control crop diseases caused by fungal ***,the anti-fungal mechanism of NNM for ***-sinensis is still *** this study,we determined the antifungal activity of NNM on ***-sinensis in vitro,with EC of 75.92 *** caused mycelial terminal deformity and swell,and induced to produce the granular contents in the mycelial *** the time of treatment was delayed to 14 h,the mycelial deformity was more obvious and the mycelial terminal represented obstructive of development,*** branches of the growing hyphae represented thinner and shorter using scanning electron *** boundary structures of hypha represented unclear,organelles became atrophy using transmission electron microscope.A time-course transcriptome of analysis revealed that NNM can significantly up-regulated 615 and 865 genes(P<0.05) and down-regulated 314 genes and 827 genes(P<0.05)for 1 hr or 14 hr at the dosage of 500 U,*** differentially expressed genes(DEGs)were verified using qRT-PCR,and the results showed that these genes represent similar expression trends with RNA-seq *** were enriched in transmembrane transport(43),oxidation-reduction process(36) and mycelium development(30) at biological process,nucleus(48),integral component of membrane(48) and cytosol(39) at cellular component,as well as zinc ion binding(30),catalytic activity(26) and oxidoreductase activity(26) at molecular *** were further enriched in biosynthesis of amino acids(23),ABC transporters(19),Tryptophan metabolism(15) for *** study will contribute to disease management of tea grey blig

关键词： Camellia sinensis Pseudopestalotiopsis camelliae-sinensis Ningnanmycin antifungal activity transcriptome

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Cladosporium cladosporioides was firstly identified from tea leaf spot disease in Yuqing,Guizhou Province,China

Cladosporium cladosporioides was firstly identified from tea...

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中国植物病理学会2019年学术年会

作者： Wang Xue yin qiaoxiu Li Dongxue Ren Yafeng Wu Xian Jiang Shilong Wang Yong Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Tea leaf spot disease was an important disease in Yuqing county,Guizhou *** symptoms were small grey dark elliptical *** lesion was gradually enlarged with irregular shape and the center of lesion appeared to be *** incidence of leaves was estimated at 46% to 52%,depending on the *** disease can seriously decrease the production and quality of tea *** this study,we isolated and identified some fungi from tea samples with the symptoms of leaf spot,and then conducted the pathogenicity *** example,Nigrospora oryzae,Nigrospora sphaerica,et al were identified from these leaves *** addition,we further identified some isolates from the samples of tea leaves,with the similar morphological *** colony of GZYQ-08-01 on PDA initially was grey olivaceous,and then changed to be grey or olivaceous black,which showed a velvety *** conidia were numerous,elliptical,limoniform,branched chains,aseptate,and measured 5.66±3.16(3.44 to 26.5) μm×3.21±0.61(2.34 to 5.02) μm(n=50).The internal transcribed spacer(ITS) regions,the translation elongation factor(TEF-1α) and actin(ACT) sequences were amplified and sequenced from the isolates,and then deposited in Genbank(Accession ***852271,MK852273,MK852272,and for GZYQ-08-01).The phylogenetic tree was constructed by PAUP v 4.0 b10 software coupled with the Maximum Parsimony *** isolate was identical to reference strains Cladosporium cladosporioides CBS15038,and the clade was supported by 100%bootstrap *** mycelial plugs on PDA or the spore suspension(1×10 spores per mL) were inoculated to the tea leaves using the methods of unwound,puncture or *** lesions were not observed on tea leaves after 7 days *** literatures reported that the fungus caused blossom blight of strawberry,leaf spot of Alstroemeria aurea,raceme blight of macadamia *** *** was involved with the formation of leaf spot in Yuqing county,Gui

关键词： Cladosporium cladosporioides identification pathogenicity test leaf spot pathogenicity mechanism

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Identifying and characterizing the circular RNAs of tea leaves inoculated with Phoma segeticola ***

Identifying and characterizing the circular RNAs of tea leav...

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中国植物病理学会2019年学术年会

作者： Li Dongxue Wang Xue yin qiaoxiu Ren Yafeng Bao Xingtao Dharmasena Dissanayake-saman-pradeep Wu Xian Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University

As a novel class of non-coding RNAs,circular RNAs(circRNAs) have been reported to play a role in various biological ***,a little attention has been focused on plant circRNAs in the study of plant-pathogen *** study aims to explore the expression profile and functional role of circRNAs in tea leaf infected by Phoma segeticola *** used comparative transcriptome analysis of the leaves of tea plant(Camellia sinensis) to identify significant differentially expressed circRNAs(DECs) from leaves infected or un-infected by the *** candidates contained more than 98% circRNA arising from the exons of genes(mismatch≤2,back-spliced junctions reads≥1 and genomic distances to back-splicing sites were less than 100 kb).We identified349 DECs including 231 up-regulated DECs and 118 down-regulated DECs(P<0.05,|log|≥1).Gene Ontology(GO) analysis indicated that DECs were most enriched in catalytic activity of molecular functions including 11 circRNAs,intracellular membrane-bounded organelle of cellular component including 6 circRNAs and response to stress of biological process including 4 *** enrichment analysis indicated that the most enriched DECs are the pathway of purine metabolism including12 circRNAs,followed by fatty acid metabolism including 10 circRNAs(P<0.05).Based on the data of circRNAs in our study,we would provide novel insights to reveal the molecular mechanisms of plantpathogen interactions.

关键词： circRNAs Phoma segeticola var.camelliae tea leaves plant-pathogen interactions

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Comparative transcriptome profiling of mRNA and lncRNA related to tea leaves infected by Phoma segeticola ***

Comparative transcriptome profiling of mRNA and lncRNA relat...

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中国植物病理学会2019年学术年会

Non-coding RNAs(ncRNAs) such as long non-coding IncRNA have been reported to play important roles in the regulation of many biological *** rapid development of omics sequencing technology has promoted the identification of thousands of lncRNAs in plant species,but the role of IncRNAs in plant-pathogen interactions remains largely *** identified significant differentially expressed genes(DEGs) andlncRNAs(DELs),and then construct lncRNA-mRNA networks using comparative transcriptome analysis of the leaves of tea plant(Camellia sinensis) infected and uninfected by Phoma segeticola ***,coupled with bioinformatics.A total of 1615 DEGs and 561 DELs(including 146 up-regulated IncRNAs and 415 down-regulated lncRNAs,P<0.05 and I log≥1) were identified between tea leaves infected and uninfected by *** *** network of co-localization was constructed for the analysis of the interaction of DEGs and *** DEGs may be cis-regulated by DELs were enriched in 10 terms containing with NADP binding and sucrose alpha-glucosidase activity,et al(P<0.05).By the enrichment analysis of KEGG,4 DELs were most enriched in the pathway of biosynthesis of amino acids(P<0.05).Integrated the analysis of the data of DEGs and DELs,6 pairs of significant co-expression pairs were screened,with 5 positively correlated pairs(COR≥0.7) and 1 negatively correlated pair(COR≤-0.7).As the differentially expressed trends of lncRNAs,IncRNA118096(MSTRG.70289.1) and lncRNA20211(MSTRG.12101.1) may play crucial roles in plant defense *** summary,the present study extends the IncRNA database of the tea leaves and these IncRNAs may provide reference on the study of plant-pathogen interactions in the future.

关键词： tea leaves Phoma segeticola var.camelliae lncRNA co-expressing

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A pathogenic fungus Pestalotiopsis trachicarpicola was firstly identified from tea leaf spot disease in Yuqing county,Guizhou Province,China

A pathogenic fungus Pestalotiopsis trachicarpicola was first...

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中国植物病理学会2019年学术年会

作者： yin qiaoxiu Ren Yafeng Li Dongxue Wang Xue Wu Xian Jiang Shilong Wang Yong Wu Xian Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Leaf spot disease caused by pathogenic fungus seriously damaged the young leaves and shoots tea,and then affects the yield and quality of tea leaves in Yuqing county,Guizhou *** was an important disease of tea plant(Camellia sinensis) in this *** symptom of the disease represents the characteristic as follow:Purple-brown or reddish-brown dots appeared on leaves in the initial stage,and then the lesions developed into round or amorphous dark *** haloed yellow lesions were around the radial *** this study,we isolated and identified some fungi from tea leaves samples with the typical symptoms,and then conducted the pathogenicity test using Koch’s *** colonies of these isolates represent whitish to pale yellow,dense aerial mycelium,circular on PDA,with fimbriate edge,and produce black fruiting bodies,with developing in concentric *** colonies display yellow on reverse *** conidiomata was globose,black,semi-immersed on *** conidiogenous cells were fusiform,hyaline,short and thin *** consist of five cells [per size(17.49-30.16) μm×(4.74-7.4) μm].Basal cell represents conic to acute,hyaline,thin-walled,verruculose,2.66 to 7.70 μ*** apical cell represents conic to subcylindrical,hyaline,verruculose,2.99 to 6.69 μ*** middle three cells represent olive color,from the top the second cell was 3.52 to7.02 μm,the third cell was 3.37 to 6.82 μm,the fourth cell was 3.11 to 7.26 μm,two to three apical appendages,length of 6.69 to 14.85 μm,and a basal appendage,length of 2.66 to 7.70 μ*** primers ITS4/ITS5 for internal transcribed spacer(ITS) region,T1/BT2 B for β-tubulin gene(TUB),and EF1-728 F/EF-2 for translation elongation factor 1-alpha(TEF-1α) was used to amplify,the sequences were deposited in *** phylogenetic tree was constructed by PAUP v 4.0 b 10 software coupled with the Maximum Parsimony method,and the isolates were identical to reference strains Pestalotiopsis trachicarpicola MFLUCC 12-0266,and the

关键词： Pestalotiopsis trachicarpicola identification pathogenicity test leaf spot

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A pathogenic fungus Didymella bellidis was firstly identified from tea leaf spot disease in Yuqing county,Guizhou Province,China

A pathogenic fungus Didymella bellidis was firstly identifie...

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中国植物病理学会2019年学术年会

作者： yin qiaoxiu Ren Yafeng Li Dongxue Wang Xue Dharmasena Dissanayake-saman-pradeep Jiang Shilong Wu Xian Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Tea leaf spot was an important disease of tea plant(Camellia sinensis) in Yuqing county,Guizhou *** disease mainly damaged the young leaves and shoots tea,and then led to a huge loss of tea *** symptoms were light brown spots,which was gradually increased in size and developed into brown,scattered,elliptical or irregular *** lesions later became greyish at the central,and then were surrounded by an apparent bright yellow *** adjacent lesions have been merged to the larger lesion,which was covered the margin of the *** this study,we isolated and identified some fungi from tea leaves samples with the symptoms of leaf spot,and then conducted the pathogenicity test using Koch’s *** colony was white to pale yellow and produced masses of flocculent aerial mycelium,with an average growth rate of 12.9±0.062 mm/d on PDA after 7 days of post-inoculation in the *** colony displayed pale yellow on reverse *** 22 days of post-inoculation,the colonies represented white to rosy *** produced on PDA were usually globose to subglobose,scattered or gregarious,olivaceous to olivaceous *** unicellular and hyaline conidia represent ellipsoidal or obovate,(4.09-9.04) μm×(1.77-4.06)μm,which usually became rounded in both ends.28 S nrDNA(LSU),internal transcribed spacer(ITS),β-tubulin 2(β-TUB2)was amplified with the primer pair LR0 R/LR7,V9 G/ITS4,Btub2 Fd/Btub4 Rd for the isolates,and then the sequences were deposited in *** parsimony phylogenetic analysis based on concatenated sequences of combined ITS(1-493),LSU(494-1897),β-TUB2(1898-2254) indicated that the isolate GZYQ-5-2-b was identical to reference strains Didymella bellidis PD 94/886,and the clade was supported by 100% bootstrap *** fulfill Koch’s postulates,the tea leaves of two tea varieties(Moss tea and Fuding Dabai cha) were inoculated with mycelial plugs on PDA or the spore suspension(1×10 spores per mL) using the methods of unwound and wou

关键词： Didymella bellidis identification pathogenicity test leaf spot

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Metabolome changes of tea leaf spot pathogen Phoma segeticola *** cultured on PDA with tea liquor

Metabolome changes of tea leaf spot pathogen Phoma segeticol...

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中国植物病理学会2019年学术年会

作者： Ren Yafeng Li Dongxue Wang Xue yin qiaoxiu Dharmasena Dissanayake-saman-pradeep Jiang Shilong Wu Xian Song Baoan Chen Zhuo Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of EducationGuizhou University College of Agriculture Guizhou University

The fungal pathogen Phoma segeticola *** causes leaf spot on tea(Camellia sinensis) in Guizhou Province,*** study the mechanism of metabolomics during the stage of the pathogen infection,we design the experiment scheme of the pathogen culturing on PDA or PDA with tea liquor(Tea variety:Fudingdabaicha).The samples were detected using Nano-LC-MS-MS(TripleTOF 5600) under the mode of positive ion and negative ion,and then the data of the metabolites was further identified and quantified using the software XCMS and *** MS and MS-MS of the metabolites were annotated using the software metaX and in-house fragment spectrum *** were12 624 features in the positive mode,and 524 could be identified by ***,1 1008 features were detected in the negative mode,and 323 could be identified by *** addition,10850 high quality features were used to quantity following condition:ratio≥2 or ratio≤1/2;q value≤0.05;VIP≥1.122 2 and 1 048 were up-or down-regulated in the positive mode,*** the negative mode,9917 high quality features were used to quantity,and 1 192 and 1 078 features were up-or down-regulated,*** to the partial least-squares discrimination analysis,R value was 1.00,and Q2 value was 0.98 in the positive *** R value was 1.00,and Q2 value was 0.99 in the negative *** different metabolites were identified in 117 pathways,such as Biosynthesis of secondary metabolites(204),Biosynthesis of antibiotics(128),Microbial metabolism in diverse environments(95) and Arachidonic acid metabolism(45) et *** data would provide important reference for the pathogenesis of tea leaf spot.

关键词： Phoma segeticola var.camelliae Camellia sinensis metabolome

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Investigating the antifungal activity and mechanism of a microbial pesticide Ningnanmycin against Phoma segeticola ***

Investigating the antifungal activity and mechanism of a mic...

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中国植物病理学会2019年学术年会

Phoma segeticola *** was isolated from tea leaf spot disease in tea garden,Shiqian county,Guizhou *** leaf spot disease affected seriously the quality and yield of *** present,there are no effective measures of prevention and ***(NNM) is a new cytosine nucleoside peptide antibiotic with high efficiency and low *** method of mycelial growth rate was used to determine the antifungal activity of NNM against *** *** strain GZSQ-4,with EC of 1 287.54 U/*** order to further study the mechanism of NNM on GZSQ-4,the mycelium treated with NNM at the dosage of EC for 1 h and 14 h was observed by optical microscope,scanning electron microscopy and transmission electron microscopy,*** was found that the mycelium swelled,the mycelial cytoplasmic density decreased,the granular content appeared and the number of mitochondria granules *** GZSQ-4 being treated by NNM at the dosage of EC for 1 h,RNA-seq showed that 1 363 differentially expressed genes(DEGs) were identified with 743 DEGs upregulated and 620 DEGs *** GO enrichment analysis,the DEGs were most enriched in the structural constituent of ribosome of molecular function,ribosome of cellular component and the oxidation-reduction process of biological process including 56,38 and 116 DEGs,respectively(P<0.05).By KEGG enrichment analysis,the DEGs were most enriched in the pathway of ribosome including 58 *** selected randomly DEGs from treatment group of NNM for 1 h were verified by *** results showed that the expression level of DEGs was consistent with the result of *** results indicated that NNM may affect the processes of translation of *** study will provide useful information for management of tea leaf spot caused by *** *** and increase our understanding of action mechanism of NNM against the pathogen.

关键词： tea disease Phoma segeticola var.camellia Ningnanmycin antifungal activity action mechanism

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