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Expression of non-structural protein NS3 gene of Bombyx mori densovirus (China isolate)

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Journal of Genetics and Genomics 2008年第4期35卷 239-244页

作者： huijuan yin Qin Yao Zhongjian Guo Fang Bao Wei Yu Jun Li Keping Chen Institute of Life Sciences Jiangsu University 301# Xuefu Road Zhenjiang 212013 China

The invertebrate parvovirus Bombyx mori Densonucleosis Virus type 3 （China isolate）, named BmDNV-3, is a kind of bidensovirus. It is a new type of virus with unique replication mechanisms. To investigate the effects of the NS3 gene during viral DNA replication, a pair of primers was designed for amplifying NS3 gene of Bombyx mori densovirus （China isolate）. Gene NS3 amplified was cloned into a prokaryotic expression vector pET-30a and the donor plasmid pFastBacHTe, respectively. The NS3 protein was expressed in Escherichia coli BL21. The pFastBacHTe-NS3 was transformed to E. coli DHIOBac. The recombinant bacmid baculoviruses （rBacmid-EGFP-NS3） isolated from the white colonies were transfected into BmN-4 cells using a transfection reagent. BraN-4 cells were infected with recombinant virus to express fusion proteins. The expression of fusion protein around 30 kDa in E. coli BL21 was identified by SDS-PAGE, Western blotting, and mass spectrometry. The expressed NS3 protein by B. mori nucleopolyhedrovirus bacmid system was confirmed by Western blotting using an anti-NS3 polyclonal antibody. And about 45 kDa protein was found. The expressed fusion protein was smaller than the expected size of EGFP-NS3, 55 kDa. Western blotting analysis indicated that EGFP-NS3 protein was expressed in infected larvae with smaller molecular size.

关键词： BmDNV NS3 protein expression

一种基于NEQR模型的高效的量子图像水印协议

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北方工业大学学报 2024年第2期36卷 45-57页

作者：孙文韬魏战红韩孟迪尹惠娟北京石油化工学院信息工程学院

量子水印以量子图像为载体，能够实现秘密信息的安全传输。本文基于新型增强量子图像表示模型（Novel Enhanced Quantum Representation of Digital Images, NEQR）提出了一种高容量的量子水印协议，该协议将大小为2~n×2~n的载体图像... 详细信息

量子水印以量子图像为载体，能够实现秘密信息的安全传输。本文基于新型增强量子图像表示模型（Novel Enhanced Quantum Representation of Digital Images, NEQR）提出了一种高容量的量子水印协议，该协议将大小为2~n×2~n的载体图像分割成22n-2个不重叠的像素块，该像素块中的像素点两两进行灰度值差值计算，依据所得差值对像素块进行分类，并对不同类别的像素块设置标志位。协议在水印嵌入前，需将2n-1×2n-1大小的水印图像扩大4倍，然后将扩大后的水印依据标志位的不同嵌入不同的载体图像最低有效位（Least Significant Bit, LSB）中。本文给出了协议实现的量子线路，合法的接收方将提取水印的量子线路作用于嵌入水印的载体图像即可获取水印信息。同时，本文在量子计算仿真环境下对协议进行了仿真实验，仿真结果说明协议具有良好的不可见性，嵌入容量较好。

关键词：量子水印 NEQR 安全性 LSB 量子信息隐藏

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

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Journal of Innovative Optical Health Sciences 2020年第6期3卷 36-46页

作者： Xiaoyue Liang Zhaohui Zou Zheng Zou Changyi Li Xiaoxi Dong huijuan yin Guohui Yan Department of Endodontics Tianjin Medical University Stomatology Hospital Tianjin 300070 P.R.China Department of Prosthodontics Tianjin Medical University Stomatology Hospital Tianjin 300070 P.R.China Institute of Biomedical Engineering Chinese Academy of Medical Sciences&Peking Union Medical College Tianjin 300192 P.R.China

The main objective of this study is to evaluate the antibacterial effect of antibacterial pho-todynamic therapy(aPDT)on Streptococcus mutans(***)biofilm model in *** selection of photosensitizers is the key step for the efficacy of photodynamic therapy(PDT).However,no studies have been conducted in the oral field to compare the functional char-acteristics and application effects of PDT mediated by various *** this re-search,the antibacterial effect of Methylene blue(MB)/650 nm laser and Hematoporphyrin monomethyl ether(HMME)/532 nm laser on *** biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two *** yield of lactic acid was analyzed by Colony forming unit(CFU)and spectrophotometry,and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy(CLSM)to evaluate the bactericidal effect on each *** on the results of CFU,the bacterial colonies formed by 30.4J/cm^(2)532nm MB-aPDT group and 30.4J/cm^(2)532nm HMME-aPDT group were significantly less than those in other groups,and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT *** acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control *** activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4 J/cm^(2) ***,aPDT suitable for energy measurement can kill *** plaque biofilm,and MB-aPDT is better than HMME-aPDT.

关键词： Caries Methylene blue(MB) Hematoporyrin monomethyl Ether(HMME) laser antimicrobial photodynamic therapy(aPDT)

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“智慧城市”虚拟仿真实验平台设计与实现

电气电子教学学报 2024年第4期46卷 233-235页

作者：王伊瑾李亚南尹辉娟任力生河北农业大学信息科学与技术学院保定071000

为解决“物联网组网技术”课程在实验教学中的不足,提高实验教学效果,提出了虚拟仿真实验教学平台,构建了包含智能小区、无人超市、车联网、城市之郊等模块的智慧城市。详细阐述了虚拟仿真实验平台的特点、细节和功能。利用三维虚拟仿真... 详细信息

为解决“物联网组网技术”课程在实验教学中的不足,提高实验教学效果,提出了虚拟仿真实验教学平台,构建了包含智能小区、无人超市、车联网、城市之郊等模块的智慧城市。详细阐述了虚拟仿真实验平台的特点、细节和功能。利用三维虚拟仿真和VR教学资源,通过交互式操作使学生直观地学习该门课程中所涉及的专业知识。

关键词：物联网交互式操作虚拟仿真

Discrimination of periodontal pathogens using Raman spectroscopy combined with machine learning algorithms

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Journal of Innovative Optical Health Sciences 2022年第3期15卷 23-35页

作者： Juan Zhang Yiping Liu Hongxiao Li Shisheng Cao Xin Li huijuan yin ying Li Xiaoxi Dong Xu Zhang School of Dentistry Tianjin Medical University 12 Qixiangtai Road Heping District Tianjin 300070P.R.China Chinese Academy of Medical Science&Peking Union Medical College Institute of Biomedical Engineering 236 Baidi Road Tianjin 300192P.R.China

Periodontitis is closely related to many systemic diseases linked by different periodontal *** unravel the relationship between periodontitis and systemic diseases,it is very important to correctly discriminate major periodontal *** realize convenient,effcient,and high-accuracy bacterial species classification,the authors use Raman spectroscopy combined with machine learning algorithms to distinguish three major periodontal pathogens Porphyromonas gingivalis(Pg),Fusobacterium nucleatum(Fn),and Aggregatibacter actinomycetemcomitans(Aa).The result shows that this novel method can successfully discriminate the three abovementioned periodontal ***,the classification accuracies for the three categories of the original data were 94.7%at the sample level and 93.9%at the spectrum level by the machine learning algorithm extra *** study provides a fast,simple,and accurate method which is very beneficial to differentiate periodontal pathogens.

关键词： Raman spectroscopy periodontal pathogen machine learning algorithm discrimination

Parallel Physics-Informed Neural Networks Method with Regularization Strategies for the Forward-Inverse Problems of the Variable Coefficient Modified KdV Equation

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Journal of Systems Science & Complexity 2024年第2期37卷 511-544页

作者： ZHOU huijuan School of Science Shanghai Maritime UniversityShanghai 201306China

This paper mainly introduces the parallel physics-informed neural networks(PPINNs)method with regularization strategies to solve the data-driven forward-inverse problems of the variable coefficient modified Korteweg-de Vries(VC-MKdV)*** the forward problem of the VC-MKdV equation,the authors use the traditional PINN method to obtain satisfactory data-driven soliton solutions and provide a detailed analysis of the impact of network width and depth on solving accuracy and ***,the author finds that the traditional PINN method outperforms the one with locally adaptive activation functions in solving the data-driven forward problems of the VC-MKdV *** for the data-driven inverse problem of the VC-MKdV equation,the author introduces a parallel neural networks to separately train the solution function and coefficient function,successfully addressing the function discovery problem of the VC-MKdV *** further enhance the network’s generalization ability and noise robustness,the author incorporates two regularization strategies into the *** amount of numerical experimental data in this paper demonstrates that the PPINNs method can effectively address the function discovery problem of the VC-MKdV equation,and the inclusion of appropriate regularization strategies in the PPINNs can improves its performance.

关键词： Data-driven forward-inverse problems parallel physics-informed neural networks regularization strategies variable coefficient modified KdV equation

Flexible mille-feuille structure electromagnetic interference shielding film with excellent thermal conductivity and Joule heating

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Nano Research 2024年第5期17卷 4544-4554页

作者： Dingwen yin huijuan Xiu Simin Wang Yanfei Pan Na Li Rui Cheng Shaoyan Huang Sha Fan Jinbao Li College of Bioresources Chemical&Materials Engineering Shaanxi University of Science&TechnologyXi'an 710021China National Demonstration Center for Experimental Light Chemistry Engineering Education Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper DevelopmentXi'an 710021China College of Material Science and Art Design Inner Mongolia Agricultural UniversityHohhot 010018China

The intelligent electronic devices have urgent demands for electromagnetic interference(EMI)shielding films with excellent heat dissipation ***,it is challenging to obtain excellent EMI shielding and thermal conductivity performances ***,inspired by mille-feuille structure,the multifunctional EMI shielding films developed by a layer-by-layer self-assembly and hot-pressing *** ingenious introduction of silver nanoparticles(AgNPs)with large specific surface area and highly conductive into the network formed by TEMPO-oxidized cellulose nanofibrils(TOCNFs)with large aspect ratio to form the TOCNFs/*** the graphene nanoplates(GNPs)with high conductivity loss distributed alternately with TOCNFs/AgNPs to construct mille-feuille structure,which had highly efficient conductive network,complete thermally conduction pathway and rich heterogeneous ***,the designed films presented high electrical conductivity of 8520 S/cm,superb EMI effectiveness(SE)of 98.05 dB,and excellent thermal conductivity of 18.82 W/(m·K).Furthermore,the films possessed outstanding Joule heating performances with low voltages,including high heating temperature(100℃),fast response time(<20 s),and impressive heating stability and ***,such high-performance EMI shielding films with fascinating thermal conductivity and Joule heating performances have substantial application in flexible electronics,electromagnetic waves shielding and thermal management.

关键词： TEMPO-oxidized cellulose nanofibrils/silver nanoparticles(TOCNFs/AgNPs) mille-feuille structure electromagnetic interference shielding thermal conductivity Joule heating

Recent Advances of Imide-Functionalized Polymer Donors for Non-fullerene Solar Cells

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Chinese Journal of Chemistry 2024年第15期42卷 1759-1780页

作者： huijuan Wang Songmin Mo Dongyan Li Qinghe Wu Department of Chemistry and Key(Guangdong-Hong Kong Joint)Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Shantou UniversityShantouGuangdong 515063China

In recent years,there has been a shift towards using non-fullerene electron acceptors in organic solar cells(osCs)as a replacement for fullerene *** change requires polymer donors that possess compatible physical properties,such as absorption range,HoMo energy level,miscibility,and ***,the high cost and poor batch-to-batch reproducibility of polymer donors also hinder future large-scale *** emphasize the need to explore alternative types of polymer *** imide-functionalized building units possess several key attributes that make their polymers highly promising for non-fullerene *** attributes include ease of synthesis,strong electron-withdrawing ability,rigid and co-planar structure,and the ability to easily tune solubility through imide side *** this review,we summarized the synthetic routes of imide building units,and the structural evolution of imide-functionalized polymer donors by focusing on the effects of polymer structure on their physical,optoelectronic,and photovoltaic *** hope that this mini-review will serve as a catalyst for future research on imide-functionalized polymers toward high-performance,cost-effective,and durable organic solar cells(oscs).

关键词： Imide Polymer donors Organic solar cells Synthetic routes Polymer structure evolution

Sulfur vacancies and heterogeneous interfaces promote high performance sodium storage of bimetallic chalcogenide hollow nanospheres

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Journal of Energy Chemistry 2024年第8期95卷 596-610,I0013页

作者： Shiyue Cao Xiaoting Xu Qiming Liu huijuan Zhu Ting Hu Key Laboratory of Artificial Micro-and Nano-structures of Ministry of Education School of Physics and TechnologyWuhan UniversityWuhan 430072HubeiChina

Transition metal sulfides have high theoretical capacities and are considered as potential anode materials for sodium-ion ***,due to low inherent conductivity and significant volume expansion,the electrochemical performance is greatly *** this study,a nickel/manganese sulfide material(Ni_(0.96)S_(x)/MnS_(y)-NC)with adjustable sulfur vacancies and heterogeneous hollow spheres was prepared using a simple *** introduction of a concentration-adjustable sulfur vacancy enables the generation of a heterogeneous interface between bimetallic sulfide and sulfur *** interface collectively creates an internal electric field,improving the mobility of electrons and ions,increasing the number of electrochemically active sites,and further optimizing the performance of Na~+*** direction of electron flow is confirmed by Density functional theory(DFT)*** hollow nano-spherical material provides a buffer for expansion,facilitating rapid transfer *** innovative discovery involves the interaction between the ether-based electrolyte and copper foil,leading to the formation of Cu_9S_5,which grafts the active material and copper current collector,reinforcing mechanical *** results in a new heterostructure of Cu_9S_5 with Ni_(0.96)S_(x)/MnS_(y),contributing to the stabilization of structural integrity for long-cycle ***,Ni_(0.96)S_(x)/MnS_(y)-NC exhibits excellent electrochemical properties following our modification *** stability performance,Ni0_(.96)S_(x)/MnS_(y)-NC demonstrates an average decay rate of 0.00944%after 10,000 cycles at an extremely high current density of 10000 mA g^(-1),A full cell with a high capacity of 304.2 mA h g^(-1)was also successfully assembled by using Na_(3)V_(2)(PO_(4))_(3)/C as the *** study explores a novel strategy for interface/vacancy co-modification in the fabrication of high-performance sodium-ion batteries electrode.

关键词： Sulfur vacancies Heterogeneous interface Interactions Sodium ion batteries