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矮牵牛涝渍胁迫相关ERF转录因子筛选与鉴定

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分子植物育种 2024年第4期22卷 1103-1113页

作者：王月悦赵安琪刘翔董婷婷尹冬梅上海应用技术大学生态技术与工程学院上海201418

ERF转录因子是植物特有的一类转录因子,属于AP2/ERF转录因子家族,调控多种生物学功能,在植物响应生物与非生物胁迫过程中起到重要作用。本研究选取矮牵牛PhERF2过表达株系(I)、RNA干扰株系(4B)为实验材料,以野生型(WT)为对照,基于涝渍处... 详细信息

ERF转录因子是植物特有的一类转录因子,属于AP2/ERF转录因子家族,调控多种生物学功能,在植物响应生物与非生物胁迫过程中起到重要作用。本研究选取矮牵牛PhERF2过表达株系(I)、RNA干扰株系(4B)为实验材料,以野生型(WT)为对照,基于涝渍处理0、3 h的转录组测序技术(RNA-Seq)获得差异表达基因数据,利用生物信息学方法分析保守基序及蛋白理化性质、构建系统进化树、进行蛋白高级结构建模、磷酸化修饰位点预测和亚细胞定位分析。结果显示,共筛选出35个涝渍胁迫相关ERF转录因子,均属于AP2亚族,通过与拟南芥及水稻相关基因进行系统进化分析,发现矮牵牛与拟南芥同源性较高。生物信息学分析表明,矮牵牛涝渍胁迫相关ERF转录因子富含酸性氨基酸,热稳定性较高且均属于亲水性蛋白,蛋白二级结构以无规则卷曲为主要组成部分,蛋白质三级结构差异不明显,含有3个保守基序,大多数NAC蛋白定位于细胞核,均含有潜在的丝氨酸(Ser)、苏氨酸(Thr)和酪氨酸(Tyr)磷酸化位点。本研究为后续探究矮牵牛涝渍胁迫相关ERF基因功能提供一定基础。

关键词：矮牵牛 ERF转录因子涝渍胁迫生物信息学

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单晶高压涡轮外环块再结晶缺陷控制

铸造工程 2024年第2期48卷 1-5页

作者：董茵尹冬梅尚相男陈杰周宝玲中国航发动力股份有限公司陕西西安710021

分析了新型发动机燃烧室高压涡轮外环块铸件的结构、铸造工艺因素对单晶铸件再结晶缺陷的影响,设计了DD5和DD6两种二代单晶高温合金定向凝固单晶铸造的试验方法,研究了典型单晶铸件DD6单晶合金高压涡轮外环块的再结晶缺陷类型和形成机... 详细信息

分析了新型发动机燃烧室高压涡轮外环块铸件的结构、铸造工艺因素对单晶铸件再结晶缺陷的影响,设计了DD5和DD6两种二代单晶高温合金定向凝固单晶铸造的试验方法,研究了典型单晶铸件DD6单晶合金高压涡轮外环块的再结晶缺陷类型和形成机理。通过采取优化工序流程、优化产品结构、增加工艺补贴等措施,将该高压涡轮外环块的再结晶缺陷从95%降低到10%以下,极大提升了单晶铸件的单晶完整性及产品合格率,降低了研制成本,提高了该铸件高温力学性能及使用可靠性。

关键词：单晶合金铸件应力再结晶

新工科与“双碳”背景下新能源转换技术的科教融汇探索与实践

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科教文汇 2024年第15期 59-62页

作者：董永军尹冬梅郭景富东北师范大学物理学院吉林长春130024 东北师范大学物理学国家级实验教学示范中心吉林长春130024 东北师范大学吉林省先进能源开发与应用创新重点实验室吉林长春130024

在新工科建设中,开展多学科交叉融合的课程体系、教研团队、实践平台、学生创新创业等建设是理工科专业发展与改革的必然趋势。面向国家创新驱动发展战略,开发新能源转换技术是实现碳达峰、碳中和目标的有效途径。在新工科和“双碳”背... 详细信息

在新工科建设中,开展多学科交叉融合的课程体系、教研团队、实践平台、学生创新创业等建设是理工科专业发展与改革的必然趋势。面向国家创新驱动发展战略,开发新能源转换技术是实现碳达峰、碳中和目标的有效途径。在新工科和“双碳”背景下,将新能源技术研究成果融入教学内容,探索多学科交叉拔尖创新人才培养的教学模式和教学方法,激发学生兴趣、挖掘学生潜力、提升学生内生动力,从而培养勇于探索、积极进取的高素质创新实践人才。

关键词：新工科双碳成果转化实践教学创新人才

Genome-wide investigation of defensin genes in peanut(Arachis hypogaea L.)reveals AhDef2.2 conferring resistance to bacterial wilt

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The Crop Journal 2022年第3期10卷 809-819页

作者： Kai Zhao Rui Ren Xingli Ma Kunkun Zhao Chengxin Qu Di Cao Qian Ma yingying Ma Fangping Gong Zhongfeng Li Xingguo Zhang dongmei yin College of Agronomy&Center for Crop Genome Engineering Henan Agricultural UniversityZhengzhou 450046HenanChina

Peanut(Arachis hypogea L.)bacterial wilt(BW)is a devastating disease caused by Ralstonia solanacearum that results in severe yield and quality *** defensins are short cysteine-rich peptides with antimicrobial *** role of defensin genes(Ah Def)in peanut is unclear.A genome-wide investigation of Ah Def genes was undertaken,and 12 identified Ah Def genes were classified into two groups containing the gamma-thionin domain formed by four disulfide pairs:Cys1-Cys8,Cys2-Cys5,Cys3-Cys6,and *** silico analysis revealed that Ah Def genes showed highly conserved architectural features and contained cis-elements associated with phytohormone signaling and defense responses.A highly resistant cultivar,H108(R)and a susceptible accession,H107(S)were tested by *** inoculation.H108(R)showed fewer symptoms than H107(S)owing to inhibition of bacterial reproduction and spread in the vascular bundles of roots and *** a transcriptomic expression profile,Ah Def genes,particularly Ah Def1.6 and Ah Def2.2,were up-regulated in H108(R)compared with H107(S)under *** infection and phytohormone *** localization showed that the Ah Def1.6 and Ah Def2.2 proteins were both expressed specifically on the plasma *** of protein fusion Ah Def2.2-YFP in Nicotiana benthamiana and peanut leaves increased resistance to ***,suggesting its role in response to BW *** Def2.2 may be valuable for peanut resistance breeding.

关键词： Peanut Bacterial wilt Defensin Expression analysis Resistance

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新时代培育强国先锋的内在逻辑与实践向度

思想理论教育 2022年第7期 55-60页

作者：尹冬梅复旦大学党委办公室上海200433

青年是实现中华民族伟大复兴的先锋力量。习近平总书记在全面把握一百年来青年运动规律和青年成长成才规律的基础上,深刻地回答了新时代培育强国先锋的重要意义、基本原则、实践路径。高校作为为党育人、为国育才的主要阵地,要坚持“党... 详细信息

青年是实现中华民族伟大复兴的先锋力量。习近平总书记在全面把握一百年来青年运动规律和青年成长成才规律的基础上,深刻地回答了新时代培育强国先锋的重要意义、基本原则、实践路径。高校作为为党育人、为国育才的主要阵地,要坚持“党管青年”的政治原则、思想铸魂的价值原则、凝聚青年的组织原则和扎根青年的工作原则,不断强化为党育人的使命感,提升理想信念的感召力,增强思想政治工作的针对性,提高育人队伍的保障度,在实现第二个百年奋斗目标新征程上培养和造就一大批堪当民族复兴大任的强国先锋。

关键词：新时代青年强国先锋

Study on the concentrated SF_6 quantitative intelligent ultrasonic detection system

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High Technology Letters 2010年第4期16卷 378-382页

作者：朱昌平 Bian Mangxi Shan Minglei yin dongmei Yu Hongzhen College of Information and Electrical Engineering China University of Mining and Technology Xuzhou 221008 P.R. China Department of Computer ＆ Information Engineering Hohai University Changzhou 213022 P.R. China

In order to realize on-line quantitative detection on SF_6 and effective control of running state of SF_6high voltage power supply system,a concentrated SF_6 quantitative ultrasonic on-line detection system hasbeen developed based on the actual demand of electric power system *** are four majorcharacteristics in this ***,the gas of maximum 64 detection points is transferred through thespecific air path to the detection devices to be detected and analyzed,thereby the electrical lines and thecomplicated installation of the collectors can be avoided;secondly,the differential technique is used toshield the influence of environmental factors,which effectively improves the accuracy of the acoustic detection ;thirdly,the SF_6 concentration is determined by the speed and phase in the ultrasonic wave transmissionprocess,therefore there is no secondary pollution for the purely physical means;finally,the matureembedded technique is applied in this system to improve its intelligence and stability.

关键词：定量检测系统 SF6气体超声波智能高压供电系统应用系统嵌入式技术需求状况

Anti-atherosclerotic effects and molecular targets of ginkgolide B from Ginkgo biloba

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Acta Pharmaceutica Sinica B 2024年第1期14卷 1-19页

作者： Weile Ye Jiaojiao Wang Peter JLittle Jiami Zou Zhihua Zheng Jing Lu Yanjun yin Hao Liu dongmei Zhang Peiqing Liu Suowen Xu Wencai Ye Zhiping Liu International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education(MOE)of China Jinan UniversityGuangzhou 510632China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research College of PharmacyJinan UniversityGuangzhou 510632China State Key Laboratory of Bioactive Molecules and Druggability Assessment Jinan UniversityGuangzhou 510632China Pharmacy Australia Centre of Excellence School of PharmacyUniversity of QueenslandWoolloongabba QLD 4102Australia Sunshine Coast Health Institute and School of Health and Behavioural Sciences University of the Sunshine CoastBirtinya QLD 4575Australia National-Local Joint Engineering Lab of Druggability and New Drugs Evaluation Guangdong Provincial Key Laboratory of New Drug Design and EvaluationSun Yat-sen UniversityGuangzhou 510006China School of Pharmacy Bengbu Medical CollegeBengbu 233030China Institute of Endocrine and Metabolic Diseases the First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China

Bioactive compounds derived from herbal medicinal plants modulate various therapeutic targets and signaling pathways associated with cardiovascular diseases(CVDs),the world’s primary cause of *** biloba,a well-known traditional Chinese medicine with notable cardiovascular actions,has been used as a cardio-and cerebrovascular therapeutic drug and nutraceutical in Asian countries for *** studies have shown that ginkgolide B,a bioactive component in Ginkgo biloba,can ameliorate atherosclerosis in cultured vascular cells and disease *** clinical relevance,several clinical trials are ongoing or being completed to examine the efficacy and safety of ginkgolide B-related drug preparations in the prevention of cerebrovascular diseases,such as ischemia ***,we present a comprehensive review of the pharmacological activities,pharmacokinetic characteristics,and mechanisms of action of ginkgolide B in atherosclerosis prevention and *** highlight new molecular targets of ginkgolide B,including nicotinamide adenine dinucleotide phosphate oxidases(NADPH oxidase),lectin-like oxidized LDL receptor-1(LOX-1),sirtuin 1(SIRT1),platelet-activating factor(PAF),proprotein convertase subtilisin/kexin type 9(PCSK9)and ***,we provide an overview and discussion of the therapeutic potential of ginkgolide B and highlight the future perspective of developing ginkgolide B as an effective therapeutic agent for treating atherosclerosis.

关键词： Cardiovascular disease Atherosclerosis Ginkgo biloba Ginkgolide B Endothelial dysfunction LOX-1 PCSK9 PAF-R antagonist

Thirty Years of Experience of Acute Flaccid Paralysis Surveillance for Polio — China, 1993–2022

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China CDC weekly 2024年第16期6卷 344-349页

作者： Hong Yang Ning Wen Chunxiang Fan Fuzhen Wang Yong Zhang Lei Cao Shuangli Zhu Lixin Hao dongmei Yan Lei Wang Quanwei Song Miao Wang Yifan Song Chao Ma Zhijie An Lance ERodewald Huaqing Wang Zundong yin National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases(NITFID) BeijingChina National Immunization Program Chinese Center for Disease Control and PreventionBeijingChina Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina

Introduction:Detecting poliovirus infections proves to be highly challenging due to their asymptomatic nature and infectious potential,highlighting the crucial importance of effective detection methods in the context of polio eradication *** many countries,including China,the primary approach for identifying polio outbreaks has been through acute flaccid paralysis(AFP)*** this study,we conducted an evaluation spanning three decades(1993–2022)to assess the effectiveness of AFP surveillance in ***:Data on all AFP cases identified since 1993 and national-level AFP surveillance system quality indicators aligned with the World Health Organization(WHO)standards were collected for *** quality indicators assess surveillance sensitivity,completeness,timeliness of detection notification,case investigation,and laboratory *** sensitivity is determined by the non-polio AFP(NPAFP)detection rate among children under 15 years of ***:Between 1993 and 2022,a total of 150,779 AFP cases were identified and *** this pool,surveillance identified 95 cases of wild poliovirus(WPV)and 24 cases due to vaccinederived *** 1995 onwards,the detection rate of NPAFP cases consistently adhered to the WHO and national standards of≥1 case per 100,000,falling between 1.38 and *** in 1997,all timeliness indicators consistently achieved the criteria of 80%,apart from the consistency in meeting standards set for the rate of positive specimens sent to the national ***:AFP surveillance has been instrumental in China’s accomplishment of maintaining a polio-free *** ongoing adherence to key performance indicators,ensuring sensitivity and prompt specimen collection,demonstrates that AFP surveillance is proficient in detecting poliovirus in *** we move into the post-eradication phase,AFP surveillance remains crucial for the sustained absence of polioviruses in the long term.

关键词： sustained prompt surveillance

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基于文本的创新测度及对企业绩效的影响研究

系统工程理论与实践 2024年第6期44卷 1896-1912页

作者：郭冬梅王继彬胡瀚清尹志锋中央财经大学经济学院北京100081 中国银行总行北京100818

本文基于中国A股上市企业的研报文本数据,使用LDA主题模型、情感分析等方法构建了文本创新指标,并通过考察其与企业绩效的相关性,验证该指标的合理性.研究发现:第一,文本创新指标具有全面性,不仅与研发投入、专利等传统创新指标具有高... 详细信息

本文基于中国A股上市企业的研报文本数据,使用LDA主题模型、情感分析等方法构建了文本创新指标,并通过考察其与企业绩效的相关性,验证该指标的合理性.研究发现:第一,文本创新指标具有全面性,不仅与研发投入、专利等传统创新指标具有高度的相关性,还能在一定程度上测度企业的非技术创新;第二,文本创新指标具有稳健性,相较于传统创新指标,文本创新指标与企业经营绩效的正相关性更稳健;第三,文本创新指标具有精准性,能部分减缓传统创新指标因测量误差、研发操纵等导致的估计偏误.本研究有益于拓展创新度量指标并据此准确估计创新对于企业经营绩效的作用效果,同时还为政府制定相关创新政策提供度量依据.

关键词：文本创新研发投入专利非技术创新企业绩效

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