检索结果-南通市图书馆

Dietary methionine sources and levels modulate the intestinal health status of broiler chickens

Animal Nutrition 2023年第4期15卷 242-255页

作者： Lu Gong Tahir Mahmood Yves Mercier Huiping Xu Xiaodan Zhang Yizhu zhao yimeng Luo Yuming Guo State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing100193China Adisseo France S.A.S 03600CommentryFrance

Given the key role of methionine in biological processes,adequate methionine should be provided to meet the nutritional ***-2-hydroxy-4-(methylthio)-butanoic acid(DL-HMTBA)has been considered as an important source of ***,the effects of different sources and levels of methionine on the intestinal health status have not been clarified *** experiment was carried out to investigate the effects of different dietary sources and levels of methionine on the intestinal epithelial barrier,inflammatory cytokines expression,ileal morphology,microbiota composition,and cecal short chain fatty acids(SCFA)*** this purpose,720 male Arbor Acre broiler chicks at 1 d old were randomly assigned to a 2×3 factorial arrangement with 2 methionine sources(DL-methionine and DLHMTBA)and 3 total sulfur amino acids(TSAA)levels(80%,100%,and 120%of Arbor Acre recommendation).The results showed that DL-HMTBA supplementation promoted intestinal physical barrier at both gene expression level of claudin-1 and serum diamine oxidase level(P<0.05),and the inflammatory cytokine IL-6 m RNA expression was down-regulated by dietary DL-HMTBA supplementation compared with the DL-methionine group(P<0.05).Meanwhile,an upregulated gene expression of claudin-1 and zonula occluden-1(ZO-1)were observed in the low-TSAA treatment on d 14(P<0.05),whereas this treatment increased the expression of IL-1βand IL-6(P<0.05).Villus height to crypt depth ratio was high(P<0.05)in the middle-level TSAA ***,DL-HMTBA supplementation optimized the microbiota of the ileum especially the relative abundance of Lactobacillus,where the digestion and absorption were completed,and elevated the concentrations of SCFA(acetate,propionate,and butyrate)in the cecal content on d 21(P<0.01).In conclusion,dietary DL-HMTBA supplementation improved the intestinal barrier function,immune homeostasis and optimized the microbiota to promote intestinal health status in broiler chickens.

关键词： Chicken Methionine 2-Hydroxy-4-(methylthio)-butanoic acid Intestinal health Intestinal barrier

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

De novo assembly of transcriptome from next-generation sequencing data

引用

Frontiers of Electrical and Electronic Engineering in China 2016年第2期4卷 94-105页

作者： Xuan Li yimeng Kong Qiong-Yi zhao Yuan-Yuan Li Pei Hao Key Laboratory of Synthetic Biology CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China The Ugniversitvof Queensland. Queensland Brain Institute. St Lucia. QI'D 4072. Ausiralia Shanghai Center for Bioinformation Technology Shanghai Industrial Technology Institute Shanghai 201203 China Key Laboratory of Molecular Virology and Immunology institute Pasteur of Shanghai Chinese Academy of SciencesShanghai 200031 China

Reconstruction of transcriptome by de novo assembly from next generation sequencing （NGS） short-sequence reads provides an essential mean to catalog expressed genes, identify splicing isoforms, and capture the expression detail of transcripts for organisms with no reference genome available. De novo transcriptome assembly faces many unique challenges, including alternative splicing, variable expression level covering a dynamic range of several orders of magnitude, artifacts introduced by reverse transcription, etc. In the current review, we illustrate the grand strategy in applying De Bruijn Graph （DBG） approach in de novo transcriptome assembly. We further analyze many parameters proven critical in transcriptome assembly using DBG. Among them, k-met length, coverage depth of reads, genome complexity, performance of different programs are addressed in greater details. A multi-k-mer strategy balancing efficiency and sensitivity is discussed and highly recommended for de novo transcriptome assembly. Future direction points to the combination of NGS and third generation sequencing technology that would greatly enhance the power of de novo transcriptomics study.

关键词： transcriptome de novo assembly De Bruijn Graph next generation sequencing k-mer length RNA splicing performance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Extraction of tight binding parameters from in-situ ARPES on the continuously doped surface of cuprates

引用

Science China(Physics,Mechanics & Astronomy) 2018年第12期61卷 25-29页

作者： YiGui Zhong yimeng Chen JianYu Guan Jin zhao ZhiCheng Rao CenYao Tang HaiJiang Liu YuJie Sun Hong Ding Beijing National Laboratory for Condensed Matter Physics and Institute of Physics Chinese Academy of SciencesBeijing 100190China School of Physics University of Chinese Academy of SciencesBeijing 100190China Collaborative Innovation Center of Quantum Matter Beijing 100190China

Recently we developed a technique of ozone/vacuum annealing to continuously change the doping level of the surface of Bi_2Sr_2CaCu_2O_(8+)and measured a nearly whole superconducting dome on one surface by in-situ angle-resolved photoemission spectroscopy [arXiv: 1805.06450]. Here we study the evolution of the electronic structures of Bi_2Sr_2CaCu_2O_(8+)xusing this technique together with tight binding fits. The tight binding parameters are extracted to study their evolution with doping.

关键词： cuprates electronic structures doping evolution tight binding ARPES

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CLK2 mRNA在早期鸡胚中的表达及定位分析

引用

家畜生态学报 2024年第2期45卷 9-12页

作者：房烨虹齐川周发亮赵鹏张若仪赵海莉任丽莉刘超肖建英刘乙蒙锦州医科大学辽宁锦州121000 锦州市妇婴医院遗传科辽宁锦州121000 山东省滨州市中心医院口腔科山东滨州251700 锦州医科大学基础医学院辽宁锦州121000

为探究早期鸡胚(孵化后约48 h内)中蛋白激酶CLK2 mRNA的表达特点及部位,用qPCR法测定早期鸡胚中CLK2 mRNA的表达,利用分子克隆技术重组CLK2/pMD18-T质粒,制作CLK2 mRNA原位杂交探针并以地高辛标记,进而通过原位杂交技术测定不同时期鸡胚... 详细信息

为探究早期鸡胚(孵化后约48 h内)中蛋白激酶CLK2 mRNA的表达特点及部位,用qPCR法测定早期鸡胚中CLK2 mRNA的表达,利用分子克隆技术重组CLK2/pMD18-T质粒,制作CLK2 mRNA原位杂交探针并以地高辛标记,进而通过原位杂交技术测定不同时期鸡胚中CLK2 mRNA的表达和分布。结果表明,早期鸡胚中CLK2 mRNA在各时期均有表达但表达量有所不同;原位杂交结果显示,在鸡胚发育的各个阶段,CLK2 mRNA的表达有所差异,且在各个部位其表达的量也各不相同,头部和尾部的表达最为集中。综上,CLK2 mRNA在鸡胚中存在且表达,其表达分布提示CLK2可能与其头部和神经系统的发育密切相关。

关键词： CLK2 早期鸡胚原位杂交

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Field-tuned magnetic structure and phase diagram of the honeycomb magnet YbCl_(3)

引用

Science China(Physics,Mechanics & Astronomy) 2021年第3期64卷 84-89页

作者： YiQing Hao HongLiang Wo yimeng Gu XiaoWen Zhang YiQing Gu ShiYi Zheng Yang zhao GuangYong Xu Jeffrey WLynn Kenji Nakajima Naoki Murai WenBin Wang Jun zhao State Key Laboratory of Surface Physics and Department of Physics Fudan UniversityShanghai 200433China NIST Center for Neutron Research National Institute of Standards and TechnologyGaithersburg 20899USA Department of Materials Science and Engineering University of MarylandCollege Park 20742USA J-PARC Center Japan Atomic Energy Agency(JAEA)Ibaraki 319-1195Japan Institute of Nanoelectronic Devices and Quantum Computing Fudan UniversityShanghai 200433China Collaborative Innovation Center of Advanced Microstructures Nanjing 210093China

We report thermodynamic and neutron diffraction measurements on the magnetic ordering properties of the honeycomb lattice magnet YbCl_(3). We find YbCl_(3) exhibits a Ne′el type long-range magnetic order at the wavevector(0, 0, 0) below TN= 600 *** magnetic order is associated with a small sharp peak in heat capacity and most magnetic entropy release occurs above the magnetic ordering temperature. The magnetic moment lies in-plane, parallel to the monoclinic a-axis, whose magnitude mYb= 0.86(3) μBis considerably smaller than the expected fully ordered moment of 2.24 μBfor the doublet crystal-field ground state. The magnetic ordering moment gradually increases with increasing magnetic field perpendicular to the ab-plane, reaching a maximum value of 1.6(2) μBat 4 T, before it is completely suppressed above ~ 9 T. These results indicate the presence of strong quantum fluctuations in YbCl_(3).

关键词： magnetic order neutron diffraction honeycomb lattice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于不同变异果型比较酸枣仁药材内在品质差异

引用

中国野生植物资源 2023年第5期42卷 16-24页

作者：索晓雄王若昱赵一萌李柯帆李晓兰杜晨晖闫艳裴香萍山西中医药大学中药与食品工程学院山西晋中030619 山西大学中医药现代研究中心山西太原030006

目的:研究不同果型酸枣仁药材质量差异,分析酸枣仁药材主要成分与其果实表型性状的相关性。方法:以山西省内8个主产区的野生酸枣果实为研究材料,对其果实的纵横径、单果重、果型指数、种子的长宽、单粒重、种子长宽比进行测定和统计分析... 详细信息

目的:研究不同果型酸枣仁药材质量差异,分析酸枣仁药材主要成分与其果实表型性状的相关性。方法:以山西省内8个主产区的野生酸枣果实为研究材料,对其果实的纵横径、单果重、果型指数、种子的长宽、单粒重、种子长宽比进行测定和统计分析;采用HPLC-UV/ELSD方法测定不同果型酸枣仁中木兰花碱、斯皮诺素、6'''-阿魏酰斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷B等5种成分含量并进行比较;利用Pearson相关性分析和灰度关联分析其表型性状与主要成分的相关性。结果:酸枣果实表型多样性丰富,其变异系数为3.81%~27.06%;不同果型酸枣仁含量测定结果表明,圆型和椭圆型酸枣中的木兰花碱、斯皮诺素、6'''-阿魏酰斯皮诺素、酸枣仁皂苷A含量均高于苹果型、纺锤型、梨型,酸枣仁皂苷B含量在不同果型之间差异较小。通过Pearson相关性分析和灰度关联分析,结果显示酸枣果实及其种子的表型性状与主要成分间具有一定关联度,且化学成分与酸枣仁的表型性状具有更高的关联度。结论:依据酸枣果实的表型性状可初步评价酸枣仁药材的质量,该研究为酸枣仁品种选育、质量评价、商品等级划分提供理论依据。

关键词：酸枣仁果型成分质量差异关联分析

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Highly Efficient Multifunctional Luminescent Radicals

引用

CCS Chemistry 2022年第2期4卷 722-731页

作者： Yihan zhao Alim Abdurahman yimeng Zhang Ping Zheng Ming Zhang Feng Li State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchunJilin 130012

Stable organic luminescent radicals are a special class of compounds integrating optical,electrical,and magnetic *** radicals not only have potential applications in the field of the organic light-emitting diodes(OLEDs)but can also be applied in the fields of fluorescence sensing,bioimaging,and so ***,due to the adverse effects of solvent polarity on the luminescent performance of radicals,no feasible approaches have been found in the literature toward fluorescent *** this work,we report two luminescent radicals,2αPyID-TTM and 2δPyID-TTM,whose emissions show high efficiency and less dependence on solvent *** radicals show remarkable protonation–deprotonation ***,2αPyID-TTM exhibits significant fluorescence quenching and colorimetric response toward Fe^(3+)in aqueous *** suggests the possibility of a fluorometric/colorimetric dual-channel probe for Fe^(3+).Moreover,an optimized OLED using 2δPyID-TTM as an emissive dopant shows pure red emission and a maximum external quantum efficiency(EQE)of 10.6%.These results show promise for luminescent radicals as fluorescent probes and electroluminescent emitters.

关键词： luminescent radical proton response fluorescence probe multifunctional materials organic light-emitting diodes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于电子鼻技术的黄精霉变快速无损鉴别研究

引用

现代中药研究与实践 2023年第2期37卷 17-22页

作者：拱健婷关佳莉李莉梁如于淑琳丛悦赵艺萌徐媛媛邹慧琴首都医科大学附属北京中医医院北京市中医药研究所北京100010 北京中医药大学中药学院北京102488

目的比较黄精与霉变黄精的气味差异,建立一种快速、准确、无损鉴别黄精霉变的方法。方法采用电子鼻对黄精样品进行气味分析,提取传感器最大响应值作为特征参数,分别利用主成分分析法、聚类分析、逐步判别分析、偏最小二乘判别分析建立... 详细信息

目的比较黄精与霉变黄精的气味差异,建立一种快速、准确、无损鉴别黄精霉变的方法。方法采用电子鼻对黄精样品进行气味分析,提取传感器最大响应值作为特征参数,分别利用主成分分析法、聚类分析、逐步判别分析、偏最小二乘判别分析建立黄精霉变快速判别模型。结果黄精霉变前后气味有显著性差异,霉变黄精在12根传感器上的响应绝对值均高于黄精;电子鼻联合化学计量学方法能够实现黄精霉变的快速判别。结论电子鼻技术能够实现黄精霉变的快速无损鉴别,为中药霉变快速检测提供一种新手段。

关键词：黄精电子鼻霉变气味快速无损鉴别

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Association between homologous recombination deficiency and outcomes with platinum and platinum-free chemotherapy in patients with triple-negative breast cancer

引用

Cancer Biology & Medicine 2023年第2期20卷 155-168页

作者： yimeng Chen Xue Wang Feng Du Jian Yue Yiran Si Xiaochen zhao Lina Cui Bei Zhang Ting Bei Binghe Xu Peng Yuan Department of Medical Oncology and Clinical Trial Center National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of VIP Medical Services National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China The Medical Department 3D Medicines Inc.Shanghai 201114China

Objective:The choice of chemotherapeutic regimen for triple-negative breast cancer(TNBC)remains *** recombination deficiency(HRD)has attracted increasing attention in informing chemotherapy *** study was aimed at investigating the feasibility of HRD as a clinically actionable biomarker for platinum-containing and platinum-free ***:Chinese patients with TNBC who received chemotherapy between May 1,2008 and March 31,2020 were retrospectively analyzed with a customized 3D-HRD *** positivity was defined by an HRD score≥30 or deleterious BRCA1/2 mutation.A total of 386 chemotherapy-treated patients with TNBC were screened from a surgical cohort(NCT01150513)and a metastatic cohort,and 189 patients with available clinical and tumor sequencing data were ***:In the entire cohort,49.2%(93/189)of patients were identified as HRD positive(40 with deleterious BRCA1/2 mutations and 53 with BRCA1/2 intact with an HRD score of≥30).In the first-line metastatic setting,platinum therapy was associated with longer median progression-free survival(mPFS)than platinum-free therapy[9.1 vs.3.0 months;hazard ratio(HR),0.43;95%confidence interval 0.22–0.84;P=0.01].Among HRD-positive patients,the mPFS was significantly longer in those treated with platinum rather than platinum-free therapy(13.6 vs.2.0 months;HR,0.11;P=0.001).Among patients administered a platinum-free regimen,HRD-negative patients showed a PFS significantly superior to that of HRD-positive patients(P=0.02;treatment-biomarker P-interaction=0.001).Similar results were observed in the BRCA1/2-intact *** the adjuvant setting,HRD-positive patients tended to benefit more from platinum chemotherapy than from platinum-free chemotherapy(P=0.05,P-interaction=0.02).Conclusions:HRD characterization may guide decision-making regarding the use of platinum treatment in patients with TNBC in both adjuvant and metastatic settings.

关键词： Homologous recombination deficiency triple-negative breast cancer platinum survival BRCA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pregnancy・lnduced Hemophagocytic Lymphohistiocytosis: A Case Report and Literature Review

引用

Chinese Medical Sciences Journal 2020年第4期35卷 371-376页

作者： Jie zhao yimeng Yang Shuhong Ming Department of Medical Oncology State Key Laboratory of Molecular OncologyNational Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100021China Department of Respiratory and Critical Care Medicine Beijing HospitalBeijing 100730China

Hemophagocytic lymphohistiocytosis(HLH)is a rare but devastating disease characterized by dysregulated immune response and hyp er *** our knowledge,pregnancy-induced HLH has been rarely reported in the literature.A 30-year・old pregnant woman presented persistent fever for 21 days since 17 weeks of *** possible etiologies such as infection,autoimmune disorder,and malignancy had been ruled out based on a series of exhaustive *** disease progressed despite the use of broadspectrum antibiotics and *** patient was diagnosed as pregnancy-induced HLH,and finally recovered completely after termination of pregnancy by caesarean and the continuous use of glucocorticoid which played a crucial part in controlling hyp er ***-induced HLH could be fatal if effective treatment was not initiated *** studies are needed to improve early diagnosis and etiology identification of HLH.

关键词： hemophagocytosis hyperinflammation pregnancy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：