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基于微流控的仿生细胞封装

Science China Materials 2024年第8期67卷 2414-2426页

作者：王经琳黄丹青方壹乐任昊桢赵远锦 Division of Hepatobiliary and Transplantation Surgery Department of General SurgeryNanjing Drum Tower Hospital(The Affiliated Hospital of Nanjing University Medical School)Nanjing 210008China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China

将细胞封装在生物材料中用于器官修复或疾病治疗是一个新兴领域.目前,微流控技术被用于精确控制封装细胞的数量、大小和支架结构.微流控技术提供了设计生物仿生形态的能力,增强封装细胞的活性和功能,从而提高疾病治疗的效果.本文报道了... 详细信息

将细胞封装在生物材料中用于器官修复或疾病治疗是一个新兴领域.目前,微流控技术被用于精确控制封装细胞的数量、大小和支架结构.微流控技术提供了设计生物仿生形态的能力,增强封装细胞的活性和功能,从而提高疾病治疗的效果.本文报道了微流控技术在仿生细胞封装方面的最新进展.我们首先简要概述了各种天然衍生的促进细胞功能的仿生结构,如受到细胞、植物、动物和自然反应启发的结构;之后讨论了基于微技术的最新细胞封装方法,并详细描述了相关过程;最后提出了仿生细胞封装在疾病治疗和临床应用中面临的挑战和未来方向.

关键词：微流控技术封装方法仿生结构仿生形态疾病治疗未来方向支架结构精确控制

A novel cartridge for nucleic acid extraction,amplification and detection of infectious disease pathogens with the help of magnetic nanoparticles

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中国化学快报:英文版 2023年第8期34卷 236-240页

作者： yile fang Yue Wang Liangxi Zhu Haoran Liu Xiangyi Su Yuan Liu Zhu Chen Hui Chen Nongyue He State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province Hunan Key Laboratory of Green Chemistry and Application of Biological NanotechnologyHunan University of TechnologyZhuzhou 412007China

Nucleic acid detection(NAD)based on real-time polymerase chain reaction(real-time PCR)is gold standard for infectious disease *** nanoparticles(MNPs)are widely used for nucleic acid extraction(NAE)because of their excellent *** technology makes automated NAD ***,most of the NAD microfluidic chips are too complex to be applied to point-of-care(POC)*** this paper,a simple-structure cartridge was developed for POC detection of infectious *** self-contained cartridge can be divided into a magnetic-controlled NAE part,a valve-piston combined fluidic control part and a PCR chip,which is able to extract nucleic acid from up to 500μL of liquid samples by MNPs and finish the detection process from“sample in”to“answer out”*** tests of the cartridges show that it met the demands of automated *** of on-cartridge detection of hepatitis B virus(HBV)demonstrated that this system has good uniformity and no cross-contamination between different cartridges,and the limit of detection(LOD)of this system for HBV in serum is 50 IU/*** detections of severe acute respiratory syndrome coronaviruses 2(SARS-CoV-2)with a concentration of 500 copies/mL were carried out on the system and 100%positive detection rate was achieved.

关键词： Nucleic acid detection Cartridge Magnetic nanoparticles Fluidic control Point-of-care testing

Selected aptamer specially combing 5-8F cells based on automatic screening instrument

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Chinese Chemical Letters 2022年第9期33卷 4208-4212页

作者： Zhukang Guo Baijiang Jin yile fang Yan Deng Zhu Chen Hui Chen Song Li Franklin Wang-Ngan Chow Polly H.M.Leung Hanming Wang Lei Cai Nongyue He State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Hunan Key Laboratory of Biomedical Nanomaterials and Devices Hunan University of TechnologyZhuzhou 412007China Department of Health Technology and Informatics The Hong Kong Polytechnic UniversityHong KongChina Guangzhou Wondfo Biotech Co. Ltd.Guangzhou 510641China Guangzhou Wondfo iCubate Biotech Co. Ltd.Guangzhou 510641China

Since the concept of aptamer emerged,many scientists have launched a rich field of research around ***,few nucleic acids aptamer which use cell as target can be put into practical *** believe that a great deal of this lies in the complexity and irreproducibility of aptamer screening experiments *** complexity is due to the cumbersome processes and the technical requirements for laboratory personnel,whereas irreproducibility arises from the fact that the starting point of such screens is nucleic acid libraries with random fragments,and that different libraries directly determine the differences or even the success or failure of screening *** complexity and irreproducibility mentioned above,in turn,lead to the inability of this experiment to unfold on a large scale,which naturally cannot lead to excellent results for practical *** response to this problem,our group has developed an instrument for automated screening of tumor cell nucleic acid aptamers and characterized the properties of nucleic acid aptamers obtained using this instrument in a comprehensive manner.

关键词： Aptamer Cell-SELEX Automatic instrument Tumor Efficient screening

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基于交联透明质酸的微针制备及性能

高分子材料科学与工程 2021年第10期37卷 153-158页

作者：庄俭杜唯佳方以勒许红黄尧康婷婷林龙北京化工大学塑料机械与塑料工程研究所北京100029

新型水凝胶微针具有安全包裹多相药物、药物持续、可控释放等优点,在微针透皮给药领域极具应用潜力。文中重点研究了交联透明质酸微针贴片的优化工艺,并对微针贴片进行了性能评价。在碱性条件下使用1,4-丁二醇二缩水甘油醚(BDDE)为交联... 详细信息

新型水凝胶微针具有安全包裹多相药物、药物持续、可控释放等优点,在微针透皮给药领域极具应用潜力。文中重点研究了交联透明质酸微针贴片的优化工艺,并对微针贴片进行了性能评价。在碱性条件下使用1,4-丁二醇二缩水甘油醚(BDDE)为交联剂对透明质酸进行交联,利用微浇注法制备交联透明质酸微针(cHA-MN),并优化其工艺。对所制备的微针贴片进行了外观形貌、力学性能、体外刺入性能及溶胀性能表征,验证其能否满足使用要求。实验结果表明,使用最优工艺制备的交联透明质酸微针贴片力学性能优良,可以顺利刺破皮肤,并实现溶胀功能。使用制备的微针搭载2.5%的右旋布洛芬药物,体外透皮扩散结果表明,微针在0~6 h释放出约38%药物,随后以一定速度缓慢释放至42 h,累计释放率约90%。

关键词：交联透明质酸水凝胶微针刺入性能溶胀性能释药性能

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儿童手足口病的数学建模和计算机模拟

数学物理学报（A辑） 2018年第5期38卷 1032-1040页

作者：李华刘三红方奕乐张兴安华中师范大学数学与统计学学院武汉430079 西安市长安区第三中学西安710000 湖北科技学院数学与统计学院湖北咸宁437100 武昌首义学院机电与自动化学院武汉430064

手足口病(HFMD)是由肠道病毒引起的传染病,患者主要是少儿,由EV71和CoxA16病毒引起.该文建立了传染病SEIHRS模型,模拟了2012-2016年手足口病患病者的数据,估计了手足口病每年的基本再生数,给出了手足口病少儿疫苗的接种率,预测了2017年... 详细信息

手足口病(HFMD)是由肠道病毒引起的传染病,患者主要是少儿,由EV71和CoxA16病毒引起.该文建立了传染病SEIHRS模型,模拟了2012-2016年手足口病患病者的数据,估计了手足口病每年的基本再生数,给出了手足口病少儿疫苗的接种率,预测了2017年手足口病患病者的数目并提出了预防控制策略.该文的结果能为该疾病的预防与控制工作提供理论依据.

关键词：手足口病基本再生数数据模拟 EV71疫苗免疫接种率

Approximately isometric lifting in quasidiagonal extensions

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Science China Mathematics 2009年第3期52卷 457-467页

作者： fang XiaoChun ZHAO yile Department of Mathematics Tongji UniversityShanghai 200092China

Let 0 → I → A → A/I → 0 be a short exact sequence of C*-algebras with A unital. Suppose that the extension 0 → I → A → A/I → 0 is quasidiagonal, then it is shown that any positive element (projection, partial isometry, unitary element, respectively) in A/I has a lifting with the same form which commutes with some quasicentral approximate unit of I consisting of projections. Furthermore, it is shown that for any given positive number ε, two positive elements (projections, partial isometries, unitary elements, respectively) $ \bar a,\bar b $ in A/I, and a positive element (projection, partial isometry, unitary element, respectively) a which is a lifting of $ \bar a $ , there is a positive element (projection, partial isometry, unitary element, respectively) b in A which is a lifting of $ \bar b $ such that ∥a?b∥ < $ \left\| {\bar a - \bar b} \right\| + \varepsilon $ . As an application, it is shown that for any positive numbers ε and $ \bar u $ in U(A/I) 0 , there exists u in U(A)0 which is a lifting of $ \bar u $ such that cel(u) < cel $ (\bar u) + \varepsilon $ .

关键词： commutativity lifting quasidiagonal extension 46L05

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微针葡萄糖传感器的制备与性能研究

传感器与微系统 2023年第1期42卷 75-78页

作者：方以勒杜唯佳林龙康婷婷孙靖尧庄俭北京化工大学机电工程学院北京100029

针对目前糖尿病血糖监测通常需要采集指血,耐受性差且监测数据不连续的问题,迫切需要开发一种无疼痛持续监测血糖水平的装置,提出了一种基于微针(MN)的电流酶基葡萄糖连续监测传感器,主要是在MN表面修饰银(Ag)层与包覆葡萄糖氧化酶(GOx... 详细信息

针对目前糖尿病血糖监测通常需要采集指血,耐受性差且监测数据不连续的问题,迫切需要开发一种无疼痛持续监测血糖水平的装置,提出了一种基于微针(MN)的电流酶基葡萄糖连续监测传感器,主要是在MN表面修饰银(Ag)层与包覆葡萄糖氧化酶(GOx)的导电高分子聚3,4-乙烯二氧噻吩(PEDOT)层来实现葡萄糖信号检测。聚乳酸MN阵列由7×7的圆锥形MN组成,MN高度和底径分别为550μm和250μm,同时,探究了熔融热压法制备MN阵列、微电子打印机喷涂Ag墨、电沉积法制备包覆GOx的PEDOT层的工艺。实验结果表明:修饰后的MN电极在生理相关范围内具有良好的线性响应(0~35 mmol/L)和响应时间(9 s),为MN无创连续监测人体生理指标的实际应用提供一定技术基础。

关键词：聚合物微针连续监测葡萄糖电沉积

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电路教学中学生工程意识和创新能力的培养

重庆电力高等专科学校学报 2016年第3期21卷 8-9,18页

作者：方奕乐屈虹万利张雪凌武昌首义学院电工电子教学基地湖北武汉430064

结合应用型本科人才培养要求,根据理论探索和实践总结,详细阐述了工程意识和创新能力的内涵以及电路教学中培养学生工程意识和创新能力的必要性和重要性,对如何在教学实践中有效培养学生工程意识和创新能力进行探讨。

关键词：电路应用型本科工程意识创新能力

The tracial topological rank of certain C*-algebras

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Science China Mathematics 2011年第11期54卷 2295-2307页

作者： fang XiaoChun ZHAO yile Department of Mathematics Tongji University Shanghai 200092 China

Let 0 →I → A →A/I →0 be a short exact sequence of C^＊-algebras with A unital. Suppose that I has tracial topological rank no more than one and A/I belongs to a class of certain C^＊-algebras. We show that A has trazial topological rank no more than one if the extension is quasidiagonal, and A has the property （P1） if the extension is tracially quasidiagonal.

关键词： tracial topological rank quasidiagonal extension tracially quasidiagonal extension