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副结核分枝杆菌重组酶介导扩增-侧向流试纸条检测方法的建立与初步应用

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畜牧兽医学报 2024年第7期55卷 3255-3260页

作者：南岳龙美贞王元智刘一朵周向梅中国农业大学动物医学院兽医公共卫生安全全国重点实验室北京100193

旨在建立一种副结核分枝杆菌(Mycobacterium avium ***,MAP)精准快速的检测方法,结合重组酶介导扩增(recombinase-aid amplification,RAA)和侧向流试纸条(lateral flow dipstick,LFD)技术,以MAP的特异性多拷贝插入元件IS900设计引物和探针,通过优化反应时间和温度等条件,建立可用于MAP现场可视化检测的RAA-LFD方法。该检测方法在35℃条件下恒温反应30 min即可实现对MAP目的基因的有效扩增。试验结果显示:该方法可特异性地检测出MAP,不与其他常见的病原发生交叉反应;对MAP标准品的最低检测限可达到10 fg·μL^(-1);使用该方法对149份牛奶样品进行检测,与传统的PCR相比,其敏感性为100%。综上,本研究成功建立了一种针对于MAP的精准快速的试纸条检测方法,并具有广泛的应用前景。

关键词：副结核分枝杆菌重组酶介导扩增侧向流试纸条

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An YIN (1959−2023)

Earth and Planetary Physics 2023年第5期7卷 603-605页

作者： XiaoFeng Liang yiduo liu FuYuan Wu State Key Laboratory of Lithospheric Evolution Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Institute of Mountain Hazards and Environment Chinese Academy of SciencesChengdu 610041China

Professor An Yin,a world-class geologist,was well known for his significant contribution to the understanding of tectonic evolution of the Earth,especially the Himalayas and Tibetan plateau(Yin A and Harrison,2000;Yin A,2006),and other planets and their satellites,such as Enceladus and Europa(Yin A et al.,2016;Leonard et al.,2018).He applied mechanical analysis to structural geology in order to understand the deformation process,not only on terrestrial planets(Yin A,2012),but also on icy Charon,the largest moon of Pluto(Chen HZ and Yin A,2022).He passed away unexpectedly in the afternoon of July 12,2023 while teaching a summer field camp at White Mountain,California,USA.

关键词： geology terrestrial plateau

宏基因组技术诊断亲内皮疱疹病毒感染致濒危亚洲象死亡病例

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畜牧兽医学报 2024年第6期55卷 2569-2577页

作者：沈青春仉伟刘青刘一朵钱佳豪张博源周师众张宜宸幸倩如丁家波周向梅张广智李秋辰李伟范学政中国农业科学院北京畜牧兽医研究所北京100193 济南市公园发展服务中心济南250099 中国农业大学动物医学院北京100193

本研究旨在借助宏基因组测序分析技术快速发现引起濒危亚洲象死亡的可能病原,再结合常规诊断技术,对大象病因进行分析和确诊,为大象相关疫病的快速诊断和预防提供参考。对死亡大象进行尸检,取心、肝、脾、肺、肾、淋巴结、肌肉、胃、十... 详细信息

本研究旨在借助宏基因组测序分析技术快速发现引起濒危亚洲象死亡的可能病原,再结合常规诊断技术,对大象病因进行分析和确诊,为大象相关疫病的快速诊断和预防提供参考。对死亡大象进行尸检,取心、肝、脾、肺、肾、淋巴结、肌肉、胃、十二指肠、空肠、结肠、盲肠、胰腺等器官制备切片,进行HE染色和病理分析;取腹股沟淋巴结进行宏基因组测序分析;使用特异性PCR方法对特征基因进行扩增和测序,经序列比对绘制进化树,分析其与其他毒株的进化关系。结果显示,死亡大象的剖检和病例切片结果符合象亲内皮疱疹病毒感染的特征,宏基因组分析结果显示,大象的腹股沟淋巴结中含有大量的象β疱疹病毒1型(即亲内皮疱疹病毒)。该大象死亡的原因可能是象亲内皮疱疹病毒感染所致,宏基因组技术对大象亲内皮疱疹病毒感染以及其他濒危动物病例的快速诊断具有良好的应用前景。

关键词：亚洲象亲内皮疱疹病毒宏基因组分析

基于联合似然函数的多扩展目标广义标签多伯努利滤波器

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电子与信息学报 2023年第4期45卷 1303-1312页

作者：刘艺多姬红兵张永权西安电子科技大学西安710077

高分辨率雷达监视系统可观测到区域内不同形状的多个扩展目标,可靠的形状估计有利于提高扩展目标跟踪性能,并可作为战场态势评估的重要依据。该文针对不同形状多扩展目标跟踪问题,提出一种基于联合似然函数的广义标签多伯努利(JL-GLMB)... 详细信息

高分辨率雷达监视系统可观测到区域内不同形状的多个扩展目标,可靠的形状估计有利于提高扩展目标跟踪性能,并可作为战场态势评估的重要依据。该文针对不同形状多扩展目标跟踪问题,提出一种基于联合似然函数的广义标签多伯努利(JL-GLMB)滤波器,可实现目标数目、航迹以及形状的精确估计。首先,将目标形状建模为星凸集,并利用非线性量测变换滤波器更新GLMB分布中的高斯分量,有效提高扩展目标状态估计精度。然后,通过对数加权融合策略,构造联合似然函数,综合衡量扩展目标和量测单元之间的相似程度。最后,基于吉布斯采样,提出快速计算扩展目标状态后验概率密度的方法,有效提高数据关联的准确率和计算效率。仿真实验结果表明,所提滤波器能够有效估计不同形状的多扩展目标状态,且在杂波环境下具有稳定的势估计。

关键词：多扩展目标跟踪随机有限集星凸集模型非线性估计

科技类事业单位职工工作满意度调查研究——以N组织为例

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今日科苑 2023年第5期 54-65页

作者：刘伊铎中国国际科技交流中心北京100081

为进一步推动事业单位人才队伍建设,研究聚焦N组织直属事业单位职工这一群体,利用理论研究与调查分析研究相结合的方式分析单位职工工作满意度。研究从工作回报、工作强度、工作内容、单位形象及内部管理、工作关系等五个维度出发,构建... 详细信息

为进一步推动事业单位人才队伍建设,研究聚焦N组织直属事业单位职工这一群体,利用理论研究与调查分析研究相结合的方式分析单位职工工作满意度。研究从工作回报、工作强度、工作内容、单位形象及内部管理、工作关系等五个维度出发,构建了事业单位工作满意度量表。分析了不同年龄、婚姻状况、工作年限、单位性质、岗位、职务、编制对于五大指标产生的影响,并建立回归方程。结果表明,工作回报、工作强度、工作内容和工作关系对于工作满意度呈正向影响,进而提出进一步提高工资整体水平,拓宽晋升渠道,增加具有吸引力的政策条件,优化内部管理系统,增强团队凝聚力,定期举办工作技能培训等措施来提升工作满意度。

关键词：工作满意度事业单位职工分析对策

Frequency Regulation of VSC-MTDC System with Offshore Wind Farms

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Journal of Modern Power Systems and Clean Energy 2024年第1期12卷 275-286页

作者： Haoyu liu Chongru liu North China Electric Power University Beijing 102206China

Frequency regulation of voltage source converter-based multi-terminal high-voltage direct current(VSC-MTDC)system with offshore wind farms enhances the frequency stability by compensating the power for a disturbed AC ***,it is difficult to reasonably allocate frequency-regulation resources due to a lack of coordination mechanisms between wind farms and the MTDC ***,it is difficult for the frequency control of the wind farms to manage changes in wind speed;and the risk of wind-turbine stalls is ***,based on the kinetic energy of wind turbines and the power margin of the converters,the frequency-regulation capability of wind turbines is evaluated,and a dynamic frequency-support scheme considering the real-time frequency-support capability of the wind turbines and system frequency evolution is proposed to improve the frequency-support performance.A power adaptation technique at variable wind speeds is developed;the active power in the frequency-support stage and restoration stage is switched according to the wind speed.A hierarchical zoning frequency-regulation scheme is designed to use the frequency-regulation resources of different links in the MTDC system with wind *** simulation results show that the novel frequency-regulation strategy maintains frequency stability with wind-speed changes and avoids multiple frequency dips.

关键词： Wind generation voltage source converter-based multi-terminal high-voltage direct current(VSC-MTDC) frequency regulation cooperative control adaptive control variable wind speed

A Novel Design of a Pre-separation Panel Structure to Protect Against the Impact of an Explosion in an Aircraft Plenum Chamber

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Acta Mechanica Solida Sinica 2024年第1期37卷 166-180页

作者： Zongxing liu Xiang Yang Jun liu School of Aeronautics Northwestern Polytechnical UniversityXi'an 710072China COMAC Shanghai Aircraft Design and Research Institute Shanghai 201210China

To reduce the damage of the pressurizing panel structure of a fuselage caused by an explosion at the“least risk bomb location”in an aircraft structure,a new pre-separation panel structure was designed to resist blast ***,the dynamic strain response and morphology of impact damage of the new pre-separation panel were measured in an impact damage ***,the commercial software LS-DYNA was used to calculate the propagation of the blast shock wave,and the results were compared with empirical equations to verify the rationality of the numerical calculation ***,the fluid–structure coupling method was used to calculate the damage process of the pre-separation panel structure under the impact of an explosion wave and an impact *** calculated results were in good agreement with the test results,which showed the rationality of the calculation method and the *** residual strength of the damaged pre-separation panel was significantly higher than that of the original damaged *** results show that the new pre-separation panel structure is reasonable and has certain significance for guiding the design of plenum chambers with strong resistance to implosion for aircraft fuselages.

关键词： Pre-separation panel structure Explosive response Residual strength Aircraft plenum chamber Least risk bomb location

Effectiveness and safety of acupuncture in treatment of pregnancyrelated symptoms:a systematic review and Meta-analysis

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Journal of Traditional Chinese Medicine 2024年第1期44卷 16-26页

作者： liu Tingting liu Tongou liu Mingfu School of Traditional Chinese Medicine Clinical Medicine Hubei University of Traditional Chinese MedicineWuhan 430000China First Clinical College of Hubei University of Traditional Chinese Medicine Wuhan 430000China Department of Acupuncture and Tuina Affiliated Hospital of Hubei University of Traditional Chinese MedicineHubei Provincial Hospital of Traditional Chinese MedicineWuhan 430000China

OBJECTIVE:To systematically evaluate the efficacy and safety of acupuncture(AM)in the treatment of pregnancyrelated symptoms such as acute vomiting during ***:We comprehensively searched the available literature up to November 2021,including Pub Med,Embase,Cochrane Library,Chinese Biomedical Literature Database,and China National Knowledge Infrastructure Database,for randomized controlled trials(RCTs)on AM for the treatment of severe vomiting,insomnia,pharyngeal and pelvic pain,mood abnormalities,and dyspepsia during ***:Sixteen RCTs with a cumulative sample size of 1178 cases were *** these,964 patients were included in the *** Meta-analysis results showed that AM was more efficient than Western medicine in treating discomfort during pregnancy[odds ratio(OR)=1.19,95%confidence interval(CI)(1.11,1.28),P<0.01].AM was better than the control group in improving the visual analog scale scores[standard mean difference(SMD)=0.62,95%CI(0.53,0.71),P<0.01].AM was superior to the control group in improving Numerical Rating Scale(NRS)symptom scores[OR=7.31,95%CI(3.36,15.94),P<0.01].There was no significant difference in adverse effects between the AM and sham-AM groups and the analgesic drug group[OR=0.70,95%CI(0.39,1.28),P=0.25],but the treatment and control groups had mild adverse effects with a low ***:AM is more effective than other treatments or pharmacotherapy alone in the treatment of pregnancy-related symptoms,and is relatively ***,the quality of the included trials was rather poor,and high-quality studies are required to confirm our findings.

关键词： acupuncture pregnancy nausea vomiting sleep initiation and maintenance disorders low back pain pelvic pain mood abnormalities Meta-analysis systematic review

Stochastic Unit Commitment with High-Penetration Offshore Wind Power Generation in Typhoon Scenarios

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Journal of Modern Power Systems and Clean Energy 2024年第2期12卷 535-546页

作者： Yanqi liu Dundun liu Hongcai Zhang State Key Laboratory of Internet of Things for Smart City and Department of Electrical and Computer Engineering University of MacaoMacao 999078China University of Macao Zhuhai UM Science and Technology Research Institute ZhuhaiChina

To tackle the energy crisis and climate change,wind farms are being heavily invested in across the *** China's coastal areas,there are abundant wind resources and numerous offshore wind farms are being *** secure operation of these wind farms may suffer from typhoons,and researchers have studied power system operation and resilience enhancement in typhoon ***,the intricate movement of a typhoon makes it challenging to evaluate its spatial-temporal *** published papers only consider predefined typhoon trajectories neglecting *** address this challenge,this study proposes a stochastic unit commitment model that incorporates high-penetration offshore wind power generation in typhoon *** adopts a data-driven method to describe the uncertainties of typhoon trajectories and considers the realistic anti-typhoon mode in offshore wind farms.A two-stage stochastic unit commitment model is designed to enhance power system resilience in typhoon *** formulate the model into a mixed-integer linear programming problem and then solve it based on the computationally-efficient progressive hedging algorithm(PHA).Finally,numerical experiments validate the effectiveness of the proposed method.

关键词： Unit commitment two-stage stochastic programming offshore wind farm typhoon