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负载碳酸钙的水包油包固乳液稳定性及相互作用研究

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食品与发酵工业 2024年第5期50卷 142-148页

作者：李功伟张杰郝佳赵伊聪许朵霞曹雁平北京工商大学食品与健康学院北京市食品添加剂工程技术研究中心北京100048

碳酸钙由于分散稳定性差,很难在食品基质中传递。水包油包固(solid-in-oil-in-water,S/O/W)三相载体乳液作为食品营养素的载体,具有制备成本低、工艺简单等优点。为提高碳酸钙在液态食品中的分散稳定性,实现较高品质利用,该文以黄原胶(x... 详细信息

碳酸钙由于分散稳定性差,很难在食品基质中传递。水包油包固(solid-in-oil-in-water,S/O/W)三相载体乳液作为食品营养素的载体,具有制备成本低、工艺简单等优点。为提高碳酸钙在液态食品中的分散稳定性,实现较高品质利用,该文以黄原胶(xanthan gum,XG)和海藻酸丙二醇酯(propylene glycol alginate,PGA)复凝聚为水相,精制猪油为油相,通过高速剪切分散构建负载碳酸钙的S/O/W钙-脂质乳液,考察PGA和XG复凝聚的界面活性和相互作用,研究不同配比PGA-XG复合物制备乳液的物理稳定性、Zeta电位、表观黏度、粒径分布及微观结构。结果表明,PGA与XG以氢键的方式产生相互作用,复凝聚后显著降低了XG在油水界面的表面张力;m(PGA)∶m(XG)=4∶6时,乳液Zeta电位为(-43.3±0.1)mV,平均粒径为(1.59±0.08)μm,有较好的物理稳定性和流变学特性,碳酸钙位于油相内部。该研究结果可丰富构建S/O/W传递系统理论,为开发新型营养素输送载体提供参考。

关键词：碳酸钙 S/O/W乳液物理稳定性黄原胶海藻酸丙二醇酯相互作用

Expansion and improvement of ChinaMu by MuT-seq and chromosome-level assembly of the Mu-starter genome

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Journal of Integrative Plant Biology 2024年第4期66卷 645-659页

作者： Lei Liang Yuancong Wang Yanbin Han yicong Chen Mengfei Li Yibo Wu Zeyang Ma Han zhao Rentao Song State Key Laboratory of Maize Bio-breeding Frontiers Science Center for Molecular Design BreedingJoint International Research Laboratory of Crop Molecular BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100091China Institute of Crop Germplasm and Biotechnology Jiangsu Provincial Key Laboratory of AgrobiologyJiangsu Academy of Agricultural SciencesNanjing 210014China Sanya Institute of China Agricultural University Sanya 572025China Hainan Yazhou Bay Seed Laboratory Sanya 572025China

ChinaMu is the largest sequence-indexed Mutator(Mu)transposon insertional library in maize(Zea mays).In this study,we made significant improvements to the size and quality of the ChinaMu *** developed a new Mu-tag isolation method Mu-Tn5-seq(MuT-seq).Compared to the previous method used by ChinaMu,MuT-seq recovered 1/3 more germinal insertions,while requiring only about 1/14 of the sequencing volume and 1/5 of the experimental *** MuT-seq,we identified 113,879 germinal insertions from 3,168 Mu-active F1 *** also assembled a high-quality genome for the Mu-active line Mu-starter,which harbors the initial active MuDR element and was used as the pollen donor for the mutation *** the Mu-starter genome,we recovered 33,662(15.6%)additional germinal insertions in 3,244(7.4%)genes in the Mu-starter *** Mu-starter genome also improved the assignment of 117,689(54.5%)germinal *** newly upgraded ChinaMu dataset currently contains 215,889 high-quality germinal *** insertions cover 32,224 pan-genes in the Mu-starter and B73Ref5 genomes,including 23,006(80.4%)core genes shared by the two *** a test model,we investigated Mu insertions in the pentatricopeptide repeat(PPR)superfamily,discovering insertions for 92%(449/487)of PPR genes in ChinaMu,demonstrating the usefulness of ChinaMu as a functional genomics resource for maize.

关键词： ChinaMu Mutator MuT-seq PPR gene Zea mays

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应激性心肌病的研究进展

心血管病学进展 2024年第7期45卷 593-597页

作者：唐琛史宏志韩红亚刘晓丽赵亦骢王琛茜张佳宜首都医科大学北京100069 解放军总医院第一医学中心普通外科医学部北京100853 首都医科大学附属北京安贞医院老年心血管病中心北京100029

应激性心肌病是以可逆性室壁运动异常为特征的一种心血管综合征,临床表现与急性冠脉综合征相似,但冠状动脉造影显示冠状动脉解剖正常。应激性心肌病的发病机制尚未完全明确,临床诊断不易,标准治疗药物存在争议,因并发症和复发风险导致... 详细信息

应激性心肌病是以可逆性室壁运动异常为特征的一种心血管综合征,临床表现与急性冠脉综合征相似,但冠状动脉造影显示冠状动脉解剖正常。应激性心肌病的发病机制尚未完全明确,临床诊断不易,标准治疗药物存在争议,因并发症和复发风险导致预后不佳。现总结应激性心肌病的流行病学、发病机制、诊断和治疗方面的最新进展,以期为改善疾病的预后提供思路。

关键词：应激性心肌病儿茶酚胺嗜铬细胞瘤 β肾上腺素受体阻滞剂

Comparison of enhancement techniques based on neural networks for attenuated voice signal captured by flexible vibration sensors on throats

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Nanotechnology and Precision Engineering 2022年第1期5卷 1-11页

作者： Shenghan Gao Changyan Zheng yicong zhao Ziyue Wu Jiao Li Xian Huang School of Precision Instruments and Opto-Electronics Engineering Tianjin University92 Weijin RoadTianjin 300072China High-Tech Institute Fan Gong-Ting South Street on the 12thQingzhou 262550China

Wearable flexible sensors attached on the neck have been developed to measure the vibration of vocal cords during ***,highfrequency attenuation caused by the frequency response of the flexible sensors and absorption of high-frequency sound by the skin are obstacles to the practical application of these sensors in speech capture based on bone *** this paper,speech enhancement techniques for enhancing the intelligibility of sensor signals are developed and *** kinds of speech enhancement algorithms based on a fully connected neural network(FCNN),a long short-term memory(LSTM),a bidirectional long short-term memory(BLSTM),and a convolutional-recurrent neural network(CRNN)are adopted to enhance the sensor signals,and their performance after deployment on four kinds of edge and cloud platforms is also *** results show that the BLSTM performs best in improving speech quality,but is poorest with regard to hardware *** improves short-time objective intelligibility(STOI)by 0.18 to nearly 0.80,which corresponds to a good intelligibility level,but it introduces latency as well as being a large *** CRNN,which improves STOI to about 0.75,ranks second among the four neural *** is also the only model that is able to achieves real-time processing with all four hardware platforms,demonstrating its great potential for deployment on mobile *** the best of our knowledge,this is one of the first trials to systematically and specifically develop processing techniques for bone-conduction speed signals captured by flexible *** results demonstrate the possibility of realizing a wearable lightweight speech collection system based on flexible vibration sensors and real-time speech enhancement to compensate for high-frequency attenuation.

关键词： Flexible electronics Vibration sensor Neural network Speech enhancement Deep learning

Tirofiban combined with Aspirin in the Treatment of Acute Penetrating Artery Territory Infarction (STRATEGY): protocol for a multicentre, randomised controlled trial

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Stroke & Vascular Neurology 2024年第1期9卷 75-81,I0047-I0052页

作者： Xiaoling Liao Shuo Feng yicong Wang Yuesong Pan Weiqi Chen Hui Qu Xingquan zhao Liping Liu Yongjun Wang Yilong Wang Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing TiantanHospitalCapital Medical UniversityBeijingChina Chinese Institute for Brain Research BeijingChina National Center for Neurological Diseases BeijingChina Advanced Innovation Center for Human Brain Protection Capital Medical UniversityBeijingChina Beijing Laboratory of Oral Health Capital Medical UniversityBeijingChina

Background Perforating artery territorial infarction(PAI)caused by branch atheromatous disease(BAD)is prone to recurrence and early progression without an effective and well-documented antiplatelet treatment ***,an adjunctive antiplatelet agent,has shown great potential to treat acute ischaemic ***,whether the combination of tirofiban and aspirin can improve the prognosis of PAI remains *** To explore an effective and safe antiplatelet regimen for reducing the risk of recurrence and early neurological deterioration(END)in PAI caused by BAD by comparing the tirofiban and aspirin combination with placebo and aspirin *** Tirofiban combined with Aspirin in the Treatment of Acute Penetrating Artery Territory Infarction(STRATEGY)trial is an ongoing multicentre,randomised,placebo-controlled trial in *** patients shall be randomly assigned to receive standard aspirin with tirofiban or placebo on the first day and standard aspirin from days 2 to *** primary endpoint is a new stroke or END within 90 *** primary safety endpoint is severe or moderate bleeding within 90 *** The STRATEGY trial will assess whether tirofiban combined with aspirin is effective and safe in preventing recurrence and END in patients with *** registration number NCT05310968.

关键词： Aspirin regimen centre

Observations on a magnetotactic bacteria-grazing ciliate in sediment from the intertidal zone of Huiquan Bay,China

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Journal of Oceanology and Limnology 2021年第6期39卷 2053-2062页

作者： Si CHEN Kaixuan CUI Wenyan ZHANG yicong zhao Tian XIAO Hongmiao PAN Wuchang ZHANG Long-Fei WU CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms(LIA-MagMC) CNRS-CASQingdao 266071China Aix Marseille Univ CNRSLCBIM2BIMMMarseille 13009France

Magnetotactic bacteria(MTB)are a group of prokaryotes having the ability to orient and swim along geomagnetic field lines because they contain intracellular magnetosomes that are synthesized through a biomineralization *** have recently also been found in unicellular eukaryotes,which are referred to as magnetically responsive protists(MRPs).The magnetosomes have three origins in *** this study,we characterized a MTB-grazing ciliated MRP that was magnetically collected from intertidal sediment of Huiquan Bay,Qingdao,*** on 18S rRNA gene sequence analysis,the ciliated MRP was tentatively identified as Uronemella parafi lificum *** transmission electron microscopy,we observed that magnetosomes having 2-3 shapes were randomly distributed within this *** X-ray spectroscopy and high-resolution transmission electron microscopy images of the magnetosomes were consistent with them being composed of *** having the same shape and mineral composition were also detected in MTB that occurred in the same environment as the ciliated *** analysis showed that the size and shape of the magnetosomes in the ciliated MRP were similar to those in *** results suggest that this ciliated MRP can graze,ingest,and digest various types of *** is certainly worth noting that this is the first record of MRPs in Asian aquatic sediment and suggesting they might be widely *** results also support the assertion that MRPs probably contribute to the ecological cycles of iron,and expand possibilities for research into the mechanism of magnetoreception in eukaryotes.

关键词： magnetically responsive protist ciliate magnetotactic bacteria magnetosome graze magnetoreception

Characterization and diversity of magnetotactic bacteria from sediments of Caroline Seamount in the Western Pacific Ocean

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Journal of Oceanology and Limnology 2021年第6期39卷 2027-2043页

作者： Kaixuan CUI Wenyan ZHANG Jia LIU Cong XU yicong zhao Si CHEN Hongmiao PAN Tian XIAO Long-Fei WU CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms(LIA-MagMC) CNRS-CASQingdao 266071China Aix Marseille Univ CNRSLCBIM2BIMMMarseille 13009France National Oceanographic Center Qingdao 266071China

Magnetotactic bacteria(MTB)are a group of microorganisms capable of orientating and swimming along magnetic fields because they contain intracellular biomineralized magnetosomes composed of magnetite(Fe 3 O 4)or/and greigite(Fe_(3)S_(4)).They are ubiquitous in freshwater,brackish,and marine habitats,and are cosmopolitan in ***,knowledge of their occurrence and distribution in seamount ecosystems is *** investigated the diversity and distribution of MTB in the Caroline Seamount(CM4).The abundance of living MTB in 12 stations in depth varying from 90 to 1545 m was 1.1×10^(3)-43.7×10^(3) inds./dm *** diverse shapes of MTB observed,magnetotactic cocci were the dominant morphotype and could be categorized into two types:1)typical cocci that appeared to have peritrichous fl agella;and 2)those characterized by having a drop-shaped form and one bundle of fl agella located at the thin/narrow end of the *** electron microscopy(TEM)analysis revealed that the magnetosomes formed by those magnetotactic cocci are magnetite(Fe 3 O 4)with octahedral crystal habit.A total of 41 operational taxonomic units(OTUs)of putative MTB(2702 reads)were acquired from nine stations,based on high-throughput *** these,40 OTUs belonged to the Proteobacteria phylum and one belonged to the Nitrospirae *** found apparent connectivity between the MTB populations on the Caroline and Kexue(Science in Chinese)seamounts,although the diversity of MTB on Caroline was much richer than on the Kexue *** results imply that the unique topography of seamounts and other as-yet unclear environmental factors could lead to evolution of different fl agella arrangements in magnetotactic cocci,and the occurrence of octahedral magnetite magnetosomes.

关键词： magnetotactic bacteria Caroline Seamount abundance diversity magnetosome

How light affect the magnetotactic behavior and reproduction of ellipsoidal multicellular magnetoglobules?

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Journal of Oceanology and Limnology 2021年第6期39卷 2005-2014页

作者： Xinxin QIAN yicong zhao Claire-Lise SANTINI Hongmiao PAN Tian XIAO Haitao CHEN Tao SONG Jinhua LI Francois ALBERTO Sophie BRUSTLEIN Long-Fei WU State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Aix Marseille University CNRSLCBCenturiIM2BIMMMarseille 13009France International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms(LIA-MagMC) CNRS-CASMarseille 13402France CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Institute of Electrical Engineering Chinese Academy of SciencesBeijing 100190China Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Aix Marseille University CenturiMarseille 13009France

Magnetotactic bacteria(MTB)synthesize intracellular magnetic organelles,magnetosomes,which consist of magnetic crystals that are enveloped in a *** are organized into a chain(s)and confer on cells a magnetic dipolar *** magnetic property allows MTB cells to align and swim along geomagnetic field lines,a movement referred to as *** MTB species change their swim direction in response to illumination by UV,violet and blue *** we analyzed the polarity of morphology,magnetism,and motion in Mediterranean multicellular magnetotactic prokaryotes,also called,magnetoglobules or *** magnetoglobules were assembled from 60-80 cells into an asymmetric ellipsoidal morphology with a relative narrow and large *** swam dominantly northward,parallel to the direction of the magnetic field,with the narrow-end as the leading *** response to a reversal in the direction of the magnetic field,they aligned quickly along the magnetic field lines and kept swimming ***,under constant illumination,385-nm UV light,magnetoglobules changed their swimming direction southward anti-parallel to the direction of the magnetic field,with the large-end as the leading *** change from a northward to southward direction occurred along with an increase of swimming speed.A minimum of 35-mW/cm^(2) irradiance of UV light was sufficient to trigger the swimming *** radiation also triggered the unidirectional division of *** these results revealed a coordination of the polarity of magnetoglobule morphology,magnetic moment,and swimming orientation,in response to magnetic and optical *** UV triggered the reversal of magnetotaxis and magnetoglobule division indicating the ecological significance of light for multicellular magnetotactic prokaryotes.

关键词： photo-response magnetic alignment coordinated swimming

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开封府彝山书院建筑庭院研究

苏州工艺美术职业技术学院学报 2022年第1期 20-23页

作者：赵宜聪开封市博物馆

书院是我国古代社会一种特殊的教育场所,中国最早具有教育属性的书院肇始于河南。开封府作为明清时期河南的省府,在发展书院教育方面处于全国的前列。本文以开封府彝山书院建筑庭院研究为切入点,探讨彝山书院作为清代河南书院的代表在... 详细信息

书院是我国古代社会一种特殊的教育场所,中国最早具有教育属性的书院肇始于河南。开封府作为明清时期河南的省府,在发展书院教育方面处于全国的前列。本文以开封府彝山书院建筑庭院研究为切入点,探讨彝山书院作为清代河南书院的代表在建筑风格与庭院布局上的特色,彰显书院建筑所蕴含注重文教和社会教化的历史价值。

关键词：彝山书院建筑风格庭院布置风景人文文化建筑