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Newly developed gas-assisted sonodynamic therapy in cancer treatment

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Acta Pharmaceutica Sinica B 2023年第7期13卷 2926-2954页

作者： Meng Pan Danrong Hu Liping Yuan Yan Yu yicong Li Zhiyong Qian State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan UniversityCollaborative Innovation Center of BiotherapyChengdu 610041China Rehabilitation Medicine Center and Institute of Rehabilitation Medicine Key Laboratory of Rehabilitation Medicine in Sichuan ProvinceWest China HospitalSichuan UniversityChengdu 610041China

Sonodynamic therapy(SDT) is an emerging noninvasive treatment modality that utilizes lowfrequency and low-intensity ultrasound(US) to trigger sensitizers to kill tumor cells with reactive oxygen species(ROS).Although SDT has attracted much attention for its properties including high tumor specificity and deep tissue penetration,its anticancer efficacy is still far from *** a result,new strategies such as gas-assisted therapy have been proposed to further promote the effectiveness of *** this review,the mechanisms of SDT and gas-assisted SDT are first ***,the applications of gas-assisted SDT for cancer therapy are introduced and categorized by gas ***,therapeutic systems for SDT that can realize real-time imaging are further ***,the challenges and perspectives of gas-assisted SDT for future clinical applications are discussed.

关键词： Sonodynamic therapy Gas therapy Cancer therapy US imaging Sonotheranostics

Comparison of enhancement techniques based on neural networks for attenuated voice signal captured by flexible vibration sensors on throats

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Nanotechnology and Precision Engineering 2022年第1期5卷 1-11页

作者： Shenghan Gao Changyan Zheng yicong Zhao Ziyue Wu Jiao Li Xian Huang School of Precision Instruments and Opto-Electronics Engineering Tianjin University92 Weijin RoadTianjin 300072China High-Tech Institute Fan Gong-Ting South Street on the 12thQingzhou 262550China

Wearable flexible sensors attached on the neck have been developed to measure the vibration of vocal cords during ***,highfrequency attenuation caused by the frequency response of the flexible sensors and absorption of high-frequency sound by the skin are obstacles to the practical application of these sensors in speech capture based on bone *** this paper,speech enhancement techniques for enhancing the intelligibility of sensor signals are developed and *** kinds of speech enhancement algorithms based on a fully connected neural network(FCNN),a long short-term memory(LSTM),a bidirectional long short-term memory(BLSTM),and a convolutional-recurrent neural network(CRNN)are adopted to enhance the sensor signals,and their performance after deployment on four kinds of edge and cloud platforms is also *** results show that the BLSTM performs best in improving speech quality,but is poorest with regard to hardware *** improves short-time objective intelligibility(STOI)by 0.18 to nearly 0.80,which corresponds to a good intelligibility level,but it introduces latency as well as being a large *** CRNN,which improves STOI to about 0.75,ranks second among the four neural *** is also the only model that is able to achieves real-time processing with all four hardware platforms,demonstrating its great potential for deployment on mobile *** the best of our knowledge,this is one of the first trials to systematically and specifically develop processing techniques for bone-conduction speed signals captured by flexible *** results demonstrate the possibility of realizing a wearable lightweight speech collection system based on flexible vibration sensors and real-time speech enhancement to compensate for high-frequency attenuation.

关键词： Flexible electronics Vibration sensor Neural network Speech enhancement Deep learning

HESSIAN MATRIX BASED SADDLE POINT DETECTION FOR GRANULES SEGMENTATION IN 2D IMAGE

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Journal of Electronics(China) 2008年第6期25卷 728-736页

作者： Chen Ken Wang yicong Yang Rener College of Information Science and Engineering Ningbo University Zhejiang 315211 China

Segmenting the touching objects in an image has been remaining as a hot subject due to the problematic complexities, and a vast number of algorithms designed to tackle this issue have come into being since a decade ago. In this paper, a new granule segmentation algorithm is developed using saddle point as the cutting point. The image is binarized and then sequentially eroded to form a gray-scale topographic counterpart, followed by using Hessian matrix computation to search for the saddle point. The segmentation is performed by cutting through the saddle point and along the maximal gradient path on the topographic surface. The results of the algorithm test on the given real images indicate certain superiorities in both the segmenting robustness and execution time to the referenced methods.

关键词： Hessian matrix Image segment Saddle point Touching objects

Dissecting PCNA function with a systematically designed mutant library in yeast

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Journal of Genetics and Genomics 2019年第6期46卷 301-313页

作者： Qingwen Jiang Weimin Zhang Chenghao Liu yicong Lin Qingyu Wu Junbiao Dai Center for Synthetic and Systems Biology School of Life Sciences Tsinghua University Beijing 100084 China Shenzhen Key Laboratory of Synthetic Genomics and Center for Synthetic Genomics Institute of Synthetic Biology Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 China Department of Biology. Brandeis University. Massachusetts 02454 USA

Proliferating cell nuclear antigen (PCNA), encoded by POL30 in Saccharomyces cerevisiae, is a key component of DNA metabolism. Here, a library consisting of 304 PCNA mutants was designed and constructed to probe the contribution of each residue to the biological function of PCNA. Five regions with elevated sen sitivity to DNA damaging reagents were identified using high-throughput phe no type screening. Using a series of genetic and biochemical analyses, we demonstrated that one particular mutant, K168A, has defects in the DNA damage tolerance (DDT) pathway by disrupting the interaction between PCNA and Rad5. Subsequent domain analysis showed that the PCNA-Rad5 interaction is a prerequisite for the function of Rad5 in DDT. Our study not only provides a resource in the form of a library of versatile mutants to study the functions of PCNA, but also reveals a key residue on PCNA (K168) which highlights the importance of the PCNA-Rad5 interaction in the template switching (TS) pathway.

关键词： Mutagenesis High-throughput DNA damage tolerance Template switching Genetic interaction

Cell-based biosensors:Towards the development of cellular monitoring

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Chinese Science Bulletin 2002年第22期47卷 1849-1856页

作者： Gaixia Xu yicong Wu Rong Li Ping Wang Weiming Yan Xiaoxiang Zheng Zhejiang Univ Dept Biomed Engn Minist Educ Biosensor Natl Special LabKey Lab BME Hangzhou 310027 Peoples R China

Cell-based biosensors (CBBs), a research hot-spot of biosensors, which treat living cells as sensing elements, can detect the functional information of biologically active analytes. They characterize with high sensitivity, excellent selectivity and rapid response, and have been applied in many fields, such as biomedicine, environmental monitoring and pharmaceutical screening. Recently cell-cultured technology, silicon microfabrication technology and genetic technology have promoted exploration of CBBs dramatically. To elucidate the novel research findings and applications of cell- based biosensors, this paper summarizes various research approaches, presents some challenges and proposes the research trends.

关键词： cell-based biosensors functional analysis cellular microenvironment electrophysiological genetically-engineered.

Asymmetric Synthesis of Anti-tuberculosis-specific Drug TBAJ-876 through Synergistic Li/Li Catalysis

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Chinese Journal of Chemistry 2023年第11期41卷 1319-1326页

作者： Jing Li Feng Gao tanveer Ahmad yicong Luo Zhenfeng Zhang Qianjia Yuan Wanbin Zhang Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs School of PharmacyShanghai Jiao Tong University800 Dongchuan RoadShanghai200240 China Frontiers Science Centre for Transformative Molecules School of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai200240 China

TBAJ-876, developed by TB Alliance, a novel anti-tuberculosis-specific drug, has entered Phase II clinical trials. Herein, the first asymmetric synthesis of TBAJ-876 has been realized using synergistic Li/Li catalysis with excellent yield of 95% and 88 : 12 er (99.6 : 0.4 er, 10 : 1 dr after simple recrystallization). Furthermore, DFT calculations and 7Li-NMR analysis illustrated the mechanism of the synergistic reaction: a chiral Li-complex activates the nucleophile to control the stereoselectivity, while the other achiral Li-complex activates the electrophile to catalyze the carbonyl addition reaction. Additionally, this protocol has been successfully carried out at 5 gram-scale, showing its industrial potential.

关键词： Asymmetric synthesis TBAJ-876 Synergistic Li Li catalysis DFT calculations Noncovalent interactions Coordination modes Lithium Enantioselectivity

Synthesis of Skipped Aminodienes by a Ni-Catalyzed Ring-Opening/Cross-Coupling Reaction of Vinylaziridines with Multifunctional Organoboronic Acids

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Chinese Journal of Chemistry 2023年第22期41卷 3003-3011页

作者： Lu Zhang Qingfeng Du yicong Luo Yuanlin Wang Feng Gao Yong Ye Wanbin Zhang Green Catalysis Center College of ChemistryZhengzhou University100 Kexue RoadZhengzhouHenan 450001China Frontiers Science Center for Transformative Molecules School of Chemistry and Chemical EngineeringShanghai Jiao Tong University 800 Dongchuan RoadShanghai 200240China

Skipped dienes are of great importance but remain challenging to synthesize because of the presence of a sp hybrid carbon between the two ***,we have developed the first nickel-catalyzed regio-,(E)-stereo-and linear-selective ring-opening/cross-coupling reaction of vinylaziridines with organoboronic acids under mild conditions to construct various skipped *** reaction exhibits wide functional-group compatibility,and could be adapted for the introduction of skipped aminodiene functionality into bioactive molecules.

关键词： DFT calculation Multifunctional organoboronic acids Ni-catalyzed Ring-opening Skipped aminodiene

ESTIMATION OF ROCK-AGGREGATE VOLUME BASED ON PCA AND LM-OPTIMIZED NEURAL NETWORK

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Journal of Electronics(China) 2009年第6期26卷 825-830页

作者： Zhao Pan Chen Ken Wang yicong Zhang Yun College of Information Science and Engineering Ningbo University Ningbo 315211 China

In granule processing industries, acquisition of particle size and shape parameters is a common procedure, and volumetric measurement is of great importance in dealing with particle sizing and gradation. To eradicate the major drawbacks with manual gauge, this paper proposes an optical approach using Back Propagation （BP） neural network to estimate the particle volume based on the two-Dimensional （2D） image information. To achieve the better network efficiency and structure simplicity, Principal Component Analysis （PCA） is adopted to reduce the dimensions of network inputs To overcome the shortcomings of generic BP network for being slow to converge and vulnerable to being trapped in local minimum, Levenberg-Marquardt （LM） algorithm is applied to achieve a higher speed and a lower error rate. The real particle data is utilized in training and testing the presented network. The experimental result suggests that the proposed neural network is capable of estimating aggregate volume with satisfactory precision and superior to the generic BP network in terms of perforxnance capacity.

关键词： Particle image Particle parameters Principal Component Analysis (PCA) Neuralnetwork Volume estimation

Steady-migration retention characteristics of peptides under gradient elution： application towards a dynamic separation method for minor-adjustments of the retention of peptides in RPLC

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Science China Chemistry 2017年第6期60卷 829-836页

作者： Min Li Yongjun Lu yicong Yang Jianjun Li Lili Wang Wei Tuo Xiaohui Ning Xin-Du Geng Institute of Modern Separation Science Shaanxi Provincial Key Laboratory Northwest University Xi'an 710069 China Xi'an Aolan Science and Technology Development Company Xi 'an 710075 China Xi'an Shiyou University Xi'an 710065 China

Minor-adjustment of the retention of peptides, induced by varying the mobile phase flow-rate(MPF-R), is a new dynamic separation method for simultaneously and rapidly identifying and improving the selectivity of hidden and overlapping peptide peaks. It can also-stabilize the reverse elution order of some pair-peaks under gradient elution in reverse phase liquid chromatography. The retention characteristics of peptides under gradient elution in RPLC was firstly found to be dominated by two variables of the steady region(SR) and migration region(MR). The changes in peptide retention induced by varying the MPF-R can be attributed to changes in the rate of bond breaking of multiple molecular interactions of peptides from the SR and of the mass transfer of peptides from the stationary phase to the mobile phase in the MR. The two dynamic variables were also found to independently depend on the type of peptide. Desirable results were obtained using six standard oligopeptides and a real sample of trypsin-digested *** is expected that the quality control of peptide drugs, high dispersion of peptide peaks in peptide mapping and "bottom-up MS"in proteomics will be improved by this method, even enabling peptide purification on a preparative scale in industry.

关键词： steady-migration retention liquid chromatography peptides dynamic separation minor-adjustment of retention elution orders