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Impact of a new teaching model on the fine cosmetic suturing operation and quantitative assessment of the training effect on plastic surgeons

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Chinese Journal of Plastic and Reconstructive Surgery 2023年第1期5卷 20-24页

作者： yichi xu Jiahua Xing Hasi Wulan Lingli Guo Department of Plastic and Reconstructive Surgery The First Medical CenterChinese PLA General HospitalBeijing 100853China School of Medicine Nankai UniversityTianjin 300071China

Background Traditional lecture-based teaching(TLT)has long been the primary method of teaching plastic suturing techniques and even surgical *** has been challenging to adapt this approach to fit the educational objectives of plastic surgery,which is a very practical ***,it is mainly teacher-led,and the course content is teacher-driven,which has disadvantages such as difficulty in motivating students and disconnection from clinical ***,we developed a video point-to-point teaching(VPT)method and teamwork-based teaching(TBT)to study the effect of the new teaching model on fine cosmetic suturing operation(FCSO)and training outcomes for plastic *** We selected 30 junior doctors from the Department of Plastic and Reconstructive Surgery of the Chinese PLA General *** trainees were randomly assigned to three groups:TLT,VPT,and *** trainees had their performances photographed,and a senior attending physician was appointed as a *** rated the process and results of FCSO according to a uniform rubric following the double-blind principle to compare the effects of different teaching modes on the trainees’FCSO and differences in training *** There was no significant effect of video recording on trainees’FCSO(P>0.05).The total scores of the first suturing in the three groups were as follows:TLT group(13.18±1.66),VPT group(13.63±1.97),and TBT group(13.50±2.26),with no significant difference among the groups(P>0.05),indicating that the starting level of the trainees in the three groups was basically the *** was no significant difference(P>0.05)between the VPT(20.30±2.17)and TBT(20.38±2.29)groups,but both of these groups were significantly better than the TLT group(16.43±1.86,P<0.01).Conclusion The TBT and VPT methods are significantly better than ***,the TBT method is more economical and optimal for teachers and better utilizes students’initiative in learning and operation

关键词： Plastic surgery teaching Traditional lecture-based teaching Video point-to-point teaching Teamwork-based teaching Training efficiency

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Innovative combined therapy for multiple keloidal dermatofibromas of the chest wall: A novel case report

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Chinese Journal of Plastic and Reconstructive Surgery 2022年第4期4卷 182-186页

作者： Jiahua Xing Yi Chen Liwu Zheng Yan Shao yichi xu Lingli Guo Department of Plastic and Reconstructive Surgery The First Medical CenterChinese PLA General HospitalBeijing 100853China School of Medicine Nankai UniversityTianjin 300071China Department of Burn and Plastic Surgery No.988 Hospital of Chinese PLAZhengzhou 450042HenanChina

Dermatofibromas are benign soft tissue tumors that predominantly affect the limbs, and more rarely the *** dermatofibroma is a rare subtype with distinct clinicopathological features and an aggressive clinical course. By researching the evolution of the disease in this study, we aimed to summarize our experience of managing a rare patient who underwent five surgeries for keloidal dermatofibroma that developed sequentially in the upper arm and chest and propose a novel treatment for keloidal dermatofibroma. We concluded that keloidal dermatofibroma involving larger areas, high tension sites, and multiple localizations can be treated using the principles of pathological scar management.

关键词： Keloidal dermatofibroma Combined therapy Chest wall Differential diagnosis Postoperative radiotherapy

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The effect of volatile anesthetics on the biologic behavior of squamous cervical cancer cells in vitro

The effect of volatile anesthetics on the biologic behavior ...

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2018年浙江省妇科肿瘤学学术年会暨温台妇产科年会

作者： Wenwen Zhang Fang xue yichi xu xueqiong Zhu Department of Obstetrics and Gynecology the Second Affiliated Hospital of Wenzhou Medical University

Objective:Cervical cancer is the second leading cause of cancer deaths in women *** anesthetics is widely used during cervical cancer ***,this study was set to investigate the effects of volatile anesthetics on proliferation,migration and invasion of squamous cervical cancer ***:The cervical cancer cell Caski was cultured and then exposed to 1%,2% or 3% sevourane and isoflurane in a purposely built cell culture chamber filled with 5% CO2 balanced with air for 2 h,*** were subsequently cultured in a normal cell incubator in 5% CO2/95% *** proliferation was determined through the CCK-8 assay and immunofluorescence *** migration and invasion potential were evaluated with the Transwell ***:There was no effect of sevoflurane on the proliferation of Caski ***,isoflurane significantly enhanced the proliferation of Caski *** was also confirmed that isoflurane,but not sevoflurane,increased the expression of Ki-67 in Caski *** sevoflurane and isoflurane exposure also promoted cell migration and invasion ability in Caski cells which was also concentration-and post-gas exposure recovery ***:This study demonstrated that both sevoflurane and isoflurane enhanced migration and invasion of squamous cervical cancer ***,but not sevoflurane,promoted the cell proliferation of squamous cervical *** these in vitro data can be reproduced in an in vivo setting together with the underlying molecular mechanisms warrant to be studied further.

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An Observation Data Driven Simulation and Analysis Framework for Early Stage <i>C. elegans</i>Embryogenesis

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Journal of Biomedical Science and Engineering 2018年第8期11卷 225-234页

作者： Dali Wang Zi Wang Xiaopeng Zhao yichi xu Zhirong Bao Department of Electric Engineering and Computer Science University of Tennessee Knoxville USA Environmental Science Division Oak Ridge National Laboratory Oak Ridge USA Department of Mechanical Aerospace and Biomedical Engineering University of Tennessee Knoxville USA Developmental Biology Program Memorial Sloan Kettering Cancer Center NYC USA

Recent developments in cutting-edge live microscopy and image analysis provide a unique opportunity to systematically investigate individual cell’s dynamics as well as simulation-based hypothesis testing. After a summary of data generation and analysis in the observation and modeling efforts related to C. elegans embryogenesis, we develop a systematic approach to model the basic behaviors of individual cells. Next, we present our ideas to model cell fate, division, and movement using 3D time-lapse images within an agent-based modeling framework. Then, we summarize preliminary result and discuss efforts in cell fate, division, and movement modeling. Finally, we discuss the ongoing efforts and future directions for C. elegans embryo modeling, including an inferred developmental landscape for cell fate, a quasi-equilibrium model for cell division, and multi-agent, deep reinforcement learning for cell movement.

关键词： C. elegans Embryogenesis Agent-Based Modeling Deep Reinforcement Learning Observation-Driven Modeling Framework 3D Live Images

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鸡蛋消费与胆固醇稳态相关性研究进展

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食品科学 2020年第7期41卷 245-254页

作者：徐亦驰彭诗怡杨芳湖北中医药大学检验学院湖北武汉430065

鸡蛋是必需脂肪、氨基酸与微量元素的来源,也是高胆固醇(200~300 mg/个)食品。其膳食的增加是否会导致胆固醇吸收增加与胆固醇动态平衡的破坏,一直是人们争论的焦点。越来越多的研究最终达成共识:一般人群中膳食胆固醇和/或蛋类摄入量... 详细信息

鸡蛋是必需脂肪、氨基酸与微量元素的来源,也是高胆固醇(200~300 mg/个)食品。其膳食的增加是否会导致胆固醇吸收增加与胆固醇动态平衡的破坏,一直是人们争论的焦点。越来越多的研究最终达成共识:一般人群中膳食胆固醇和/或蛋类摄入量与心血管疾病(cardiovascular diseases,CVDs)发病率之间仅具有微弱的相关性或者不相关,鸡蛋中有功能成分调控胆固醇的吸收转运及代谢。但又引发有关新的争论,即富含磷脂的鸡蛋是否通过微生物发酵产生的代谢产物三甲胺-N-氧化物(trimethylamine-N-oxide,TMAO)促进血管动脉粥样硬化的发生与发展。本综述旨在总结胆固醇与鸡蛋的饮食建议、胆固醇代谢的稳态平衡、胆固醇与鸡蛋的相关流行病学研究、鸡蛋摄入影响胆固醇稳态的相关机制以及TMAO与CVDs相关性等方面的最新研究进展。

关键词：鸡蛋消费胆固醇代谢平衡心血管疾病三甲胺-N-氧化物

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人类发育细胞蓝图的现状和挑战

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遗传 2024年第10期46卷 760-778页

作者：阙亦宸刘清泉徐一驰复旦大学基础医学院医学系统生物学系和前沿创新中心上海200032

阐明人类胚胎发育的分子调控机制一直是科学家们努力攻克的重大生物学问题之一。然而,由于人类胚胎样本稀少、样本解剖困难、器官结构复杂等因素,解析人类胚胎发育机制面临重重挑战。近年来,随着单细胞组学技术的迅猛发展,人们可以在中... 详细信息

阐明人类胚胎发育的分子调控机制一直是科学家们努力攻克的重大生物学问题之一。然而,由于人类胚胎样本稀少、样本解剖困难、器官结构复杂等因素,解析人类胚胎发育机制面临重重挑战。近年来,随着单细胞组学技术的迅猛发展,人们可以在中心法则的各个环节,系统地解析细胞分化过程中的动态变化,并在空间维度上进行观测与研究,加快了构建人类发育细胞图谱的进程,最终实现对人类发育的细胞分类体系、命运轨迹和三维动态变化的刻画。本文首先介绍了绘制人类发育细胞图谱所使用的单细胞组学技术,然后总结了胚胎发育图谱的最新进展并提出了该领域目前面临的主要问题和挑战,最后对如何利用人类胚胎发育图谱解决生物学和医学的关键问题进行了探讨,以期为利用单细胞组学技术构建并应用人类发育细胞蓝图提供更好的指导。

关键词：人类胚胎发育单细胞组学细胞图谱

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Inhibition of Osteoarthritis in Rats by Electroporation with Interleukin-1 Receptor Antagonist

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Journal of Biomedical Science and Engineering 2016年第7期9卷 323-336页

作者： Zhen Sun Heyong Yin Xiaoming Yu xun Sun Bo Xiao yichi xu Zhiguo Yuan Haoye Meng Jiang Peng Changlong Yu Yu Wang Quanyi Guo Aiyuan Wang Shibi Lu Beijing Key Lab of Regenerative Medicine in Orthopedics Key Lab of Musculoskeletal Trauma & War Injuries General Hospital of People’s Liberation Army Beijing China Institute of Sport Medicine Peking University Third Hospital Beijing China

Gene therapy constitutes a promising strategy for the treatment of osteoarthritis (OA). We assessed the use of electroporation (EP) of non-viral gene vectors, and compared its efficacy with that of adeno-associated virus (AAV) vectors. EP- and AAV-mediated delivery of human interleukin-1 receptor antagonist (hIL-1Ra) was localized performed in the joints of rats following induction of OA. mRNA levels for hIL-1Ra, IL-1β, TNF-α, MMP-13 and ADAMTS-4 in the cartilage and synovial tissues were analyzed. Structural analyses of the subchondral bone at the medial femoral condyle were performed by Micro-CT after treatment. Knee joint specimens were staining with hematoxylin and eosin and Saffron O. Induction of hIL-1Ra by both EP and AAV inhibited inflammatory-induced sub-chondral bone reconstruction, and effectively suppressed IL-1β activity, as evidenced by decreased expression of MMP-13 and ADAMTS-4. Histological analyses revealed significant protection of cartilage, proteoglycan by EP and AAV. hIL-1Ra expression was similar in both the EP and AAV groups. Notably, this gene is not easier degraded transduced by EP compared with AAV. Taken together, these results show that EP offers transfection efficiency comparable to that of AAV, with the potential for longer gene expression, making EP a promising candidate for efficient non-viral delivery of OA gene therapy.

关键词： Electroporation Interleukin-1 Receptor Antagonist Adeno-Associated Virus Gene Therapy Osteoarthritis Cartilage Synovium

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中西医结合治疗老年2型糖尿病合并骨质疏松症临床观察

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中国中医药现代远程教育 2022年第12期20卷 67-69页

作者：许慧杨婉妤许一驰中国医科大学附属第一医院老年医学科辽宁沈阳110001

目的观察中西医结合治疗老年2型糖尿病合并骨质疏松症的临床疗效。方法纳入中国医科大学附属第一医院2018年1月—2020年3月收治的2型糖尿病合并骨质疏松症患者130例,随机分为观察组和对照组,各65例。对照组采用西医常规治疗,观察组在对... 详细信息

目的观察中西医结合治疗老年2型糖尿病合并骨质疏松症的临床疗效。方法纳入中国医科大学附属第一医院2018年1月—2020年3月收治的2型糖尿病合并骨质疏松症患者130例,随机分为观察组和对照组,各65例。对照组采用西医常规治疗,观察组在对照组基础上加用中药方剂辨证施治。比较2组治疗前后血液生化指标、骨密度及临床总有效率。结果治疗前2组空腹血糖(FPG)、糖化血红蛋白(HbAlc)、血钙水平及桡骨、股骨、腰椎骨密度比较,差异无统计学意义(P>0.05)。治疗后2组FPG、HbAlc水平显著降低,桡骨、股骨、腰椎骨密度升高,且观察组优于对照组,差异均有统计学意义(P<0.05);观察组临床总有效率86.15%(56/65)显著高于对照组63.08%(41/65),差异有统计学意义(P<0.05)。结论中西医结合治疗老年2型糖尿病合并骨质疏松症效果显著,能有效缓解临床症状,控制血糖,增加骨密度,值得临床推广。

关键词：骨痹 2型糖尿病骨质疏松中西医结合疗法老年病骨密度

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电喷雾法制备海藻酸盐-明胶-脂肪干细胞微球及其修复关节软骨损伤的可行性

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中国组织工程研究 2021年第28期25卷 4473-4479页

作者：廖思达孟昊业李俊康徐亦驰李获田萧羽冯勇汪爱媛彭江中国人民解放军医学院北京市100853 中国解放军总医院第一医学中心骨科研究所骨科再生医学北京市重点实验室全军骨科战创伤重点实验室北京市100853

背景:微球可作为细胞递送系统,通过注射或者与其他支架相结合的方式使细胞定位于软骨损伤处使其再生。目的:采用电喷雾法将脂肪源性干细胞负载至海藻酸盐-明胶微球中,评估微球中的细胞活性、增殖和成软骨分化能力。方法:使用电喷雾技术... 详细信息

背景:微球可作为细胞递送系统,通过注射或者与其他支架相结合的方式使细胞定位于软骨损伤处使其再生。目的:采用电喷雾法将脂肪源性干细胞负载至海藻酸盐-明胶微球中,评估微球中的细胞活性、增殖和成软骨分化能力。方法:使用电喷雾技术制备含不同质量浓度明胶(0,5,15,25 g/L)的海藻酸盐微球,光学显微镜下观察微球的外观,选择形成完整微球的明胶质量浓度用于以下实验。使用电喷雾技术制备含脂肪源性干细胞的海藻酸盐微球与海藻酸盐微球-明胶微球,光学显微镜下观察成球效果。将含细胞的两种微球培养于旋转细胞培养系统内,在设定的时间点检测微球内细胞的增殖能力、活性与成软骨能力。结果与结论:①加入0,5 g/L的明胶可制备出完整的微球,微球直径分别为(365.85±16.88),(358.85±23.97)μm,两种微球直径比较差异无显著性意义(P﹥0.05),后续实验选择明胶质量浓度为5 g/L;②加入脂肪源性干细胞不影响藻酸盐微球与海藻酸盐微球-明胶微球的成球性与微球直径;③含细胞海藻酸盐微球-明胶微球培养7,14 d的细胞增殖数量、活细胞比例均高于含细胞海藻酸盐微球(P<0.05);④成软骨诱导培养后,含细胞海藻酸盐微球-明胶微球诱导7,14,21 d的糖胺多糖含量高于含细胞海藻酸盐微球(P<0.05);⑤结果表明,海藻酸盐-明胶微球更易促进脂肪源性干细胞的增殖并保持细胞活性,是软骨组织工程领域中一种具有巨大潜力的生物材料。

关键词：骨材料海藻酸盐明胶软骨组织工程生物材料电喷雾微载体

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载VEGF/万古霉素的多层缓释微球对人胎盘源间充质干细胞增殖与成骨分化的影响

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吉林大学学报（医学版） 2017年第2期43卷 250-254,I0003页

作者：柳康王敬龙杨军星宋立杰徐一驰赵楚翘王瑶刘志辉王博蔚吉林大学口腔医院修复科吉林长春130021 温州医科大学口腔医学院附属口腔医院牙周病科浙江温州325000 吉林大学第二医院妇产科吉林长春130041

目的:观察载血管内皮生长因子(VEGF)/万古霉素的多层海藻酸盐壳聚糖缓释微球对人胎盘源间充质干细胞(HPMSCs)增殖与成骨分化的影响,为其在组织工程修复骨缺损中的临床应用提供理论基础。方法:根据前期基础制备缓释微球,将从人胎盘组织... 详细信息

目的:观察载血管内皮生长因子(VEGF)/万古霉素的多层海藻酸盐壳聚糖缓释微球对人胎盘源间充质干细胞(HPMSCs)增殖与成骨分化的影响,为其在组织工程修复骨缺损中的临床应用提供理论基础。方法:根据前期基础制备缓释微球,将从人胎盘组织中分离培养的一部分HPMSCs分为载药微球组(载药微球+HPMSCs)、空载微球组(空载微球+HPMSCs)和无球组(仅HPMSCs)。采用CCK-8试剂盒检测HPMSCs增殖率。取另一部分HPMSCs分为载药微球诱导组(载药微球+HPMSCs+成骨诱导液)、空载微球诱导组(空载微球+HPMSCs+成骨诱导液)、无球诱导组(HPMSCs+成骨诱导液)和非诱导组(HPMSCs+PBS)。孵育21d后分别采用茜素红染色及碱性磷酸酶(ALP)试剂盒检测HPMSCs钙盐沉积与ALP活性。结果:与无球组比较,载药微球组和空载微球组共培养后HPMSCs增殖率均无明显改变(P>0.05)。成骨诱导后茜素红染色,载药微球诱导组钙盐沉积明显多于空载微球诱导组和无球诱导组;载药微球诱导组细胞内ALP活性明显高于空载微球诱导组和无球诱导组(P<0.05),空载微球诱导组细胞ALP活性高于无球诱导组(P<0.05)。结论:载VEGF/万古霉素的多层海藻酸盐壳聚糖缓释微球对HPMSCs增殖活性无明显影响,且能提高HPMSCs的成骨分化能力。

关键词：干细胞血管内皮生长因子万古霉素微球细胞增殖成骨碱性磷酸酶

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