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Trichoderma viride F7 improves peanut performances while remediating cadmium-contaminated soil with microplastics

Pedosphere 2024年第5期34卷 971-980页

作者： Xin SONG Jianpeng JIN Hongliang yiN Tingjuan WANG Haiying ZONG Fangli WANG Jun liu Xiaoli HUANG Bin WANG Chao CHAI Zhi LI Dongsheng liu Xuexia WANG Ningning SONG School of Resources and Environment Qingdao Agricultural University Central Laboratory Qingdao Agricultural University Institute of Soil Fertilizer and Agricultural Water Saving Xinjiang Academy of Agricultural Sciences Institute of Plant Nutrition and Resources Beijing Agricultural Forestry Academy Sciences

Microplastics(MPs) and cadmium(Cd) in soil have serious negative impacts on plant growth. As a bioremediator, Trichoderma viride may modulate the impacts of MPs and/or Cd on plant growth and heavy metal uptake. In this study, T. viride F7 was used to treat Cd-contaminated soil with and without the presence of polylactic acid MPs to investigate its effects on peanut growth, Cd uptake efficiency, and soil properties. Results showed that F7 increased soil pH,dissolved organic carbon, cation exchange capacity, microbial biomass nitrogen, and microbial biomass carbon, which resulted in increases of 2.10%–19.83% in peanut biomass and mitigated the negative effects of MPs and Cd on peanut growth. Trichoderma viride F7 reduced the soil bioavailable Cd and plant Cd concentrations by 16.68%–34.20% and 5.37%–25.84%, respectively. Regression path analysis showed that F7 could improve peanut performances by altering soil properties and reducing peanut Cd concentrations in MP-Cd-contaminated soil. Soil fungal community analysis indicated that the microbial community was altered via F7 inoculation, which antagonized Fusarium and promoted peanut growth. This study reveals the mechanisms of F7 in mitigating Cd contamination in the soil with the presence of MPs, which provides new ideas for managing MP-heavy metal-contaminated soil and improving soil health.

关键词： bioremediation compound pollution emerging pollutant heavy metal polyethylene soil health

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Asymmetric reduction of conjugated C=C bonds by immobilized fusion of old yellow enzyme and glucose dehydrogenase

Green Synthesis and Catalysis

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Green Synthesis and Catalysis 2024年第2期5卷 80-87页

作者： Yongxing Li Pengqian Luan Lele Dong Jianqiao liu Luying Jiang Jing Bai Fufeng liu Yanjun Jiang School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China College of Food Science and Biology Hebei University of Science&Technology26 Yuxiang StreetYuhua DistrictShijiazhuang 050018China Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education Tianjin Key Laboratory of Industrial MicrobiologyCollege of BiotechnologyTianjin University of Science&TechnologyTianjin 300457China

Asymmetric reduction of the conjugated C=C bonds by the old yellow enzymes(OYEs)presents a promising field in the synthesis of chiral ***,few natural OYEs have been applied in large-scale applications due to the requirement of costly NADPH and low operational ***,a stable and efficient fusion of YqjM from Bacillus subtilis and glucose dehydrogenase(GDH)from Bacillus megaterium was constructed to stereoselectively reduce the conjugated C=C bonds in a self-sufficient continuous *** effects of the enzyme order and different linkers on the fusions were investigated by structural analysis and all-atom molecular dynamics *** best fusion YqjM_G_GDH gave 98% conversion of 100 mmol/L 2-methylcyclopentenone with an excellent ee value(>99%)in 3 h,while the mixture of individual enzymes only obtained 68% conversion after more than 8 *** improved substrate conversion of YqjM_G_GDH fusion was probably attributed to the increased flexibility of each fused enzyme and the shortening of the diffusion distance of NADPH regenerated.A one-pot process was designed to purify and immobilize the fusion on the Ni2t-nitrilotriacetic acid functionalized magnetic mesoporous silica *** resulting immobilized biocatalyst not only catalyzed the asymmetric reduction of various α,β-unsaturated ketones(20 mmol/L)continuously with only 50μmol/L NADPt to initiate the whole process,but also retained more than 82%of the initial activity after seven cycles,serving as a good candidate for the industrial applications.

关键词： Enzyme fusion Old yellowenzymes Asymmetricreduction C=C bond Cofactor regeneration Immobilization

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政府补贴对专精特新中小企业创新能力的影响--来自长三角地区的证据

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北京联合大学学报（人文社会科学版） 2024年第2期22卷 34-47页

作者：杨宜刘祖娴赵睿北京财贸职业学院北京101101 对外经济贸易大学国际经济贸易学院北京100029 北京联合大学商务学院北京100025

专精特新中小企业是我国创新创造的主力军,现有研究证明补贴是政府扶持科技创新的有效手段。长三角地区是中国中小企业集聚地,也是创新能力最强的区域之一,且区域内产业发展具有差异化特点,因此本文选取2016—2020年浙江、上海、江苏、... 详细信息

专精特新中小企业是我国创新创造的主力军,现有研究证明补贴是政府扶持科技创新的有效手段。长三角地区是中国中小企业集聚地,也是创新能力最强的区域之一,且区域内产业发展具有差异化特点,因此本文选取2016—2020年浙江、上海、江苏、安徽四省市109家A股上市的专精特新中小企业作为研究对象,采用固定效应模型和泊松模型分析政府补贴对专精特新中小企业创新能力的作用和区域异质性。结果表明:总体上政府补贴对企业的创新能力提升有正向影响,政府补贴通过缓解融资约束、促进企业创新合作机制激励专精特新企业创新,但具体效果因产权性质、行业性质和知识产权保护程度不同而存在区域差异性和滞后性。本文建议通过针对各区域位于重点产业链的专精特新企业进行精准补贴,将发明专利数、专利引用数等创新质量指标纳入补贴绩效考核指标体系,重点资助非国有企业与高科技行业的专精特新企业,尤其注重补贴可持续创新的成长型中小企业,强化知识产权保护,保证补贴的连续性,以提升政府补贴对创新的引导作用。

关键词：政府补贴专精特新中小企业创新能力技术改进

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Toward quantum unmanned systems

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Science China(Technological Sciences) 2024年第7期67卷 2277-2280页

作者： CHENG ShuMing QI HongSheng SUN Yue liu LiJun XIE ShaoRong REN XiaoQiang Department of Control Science and Engineering Tongji UniversityShanghai 200092China Academy of Mathematics and Systems Science Chinese Academy of SciencesBeijing 100190China Cipher Cloud Core Ltd. Beijing 100085China College of Mathematics and Computer Science Shanxi Normal UniversityLinfen 041081China School of Computer Engineering and Science Shanghai UniversityShanghai 200444China School of Mechatronic Engineering and Automation Shanghai UniversityShanghai 200444China

1 Quantum information technology Quantum information technology utilizes physical systems at the microscopic level, such as photon, atom, ion, and superconducting, to accomplish information-processing tasks that are impossible for the classical macroscopic world. During the past decade, significant process has been achieved in the pursuit of quantum technology into practical applications,generating great research interest from various domains, with the potential to radically change our information infrastructure [1–3].

关键词： quantum utilize generating

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化学教育中学习轨迹的概念及内涵分析

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化学教育（中英文） 2024年第9期45卷 2-8页

作者：刘祎玮卢姗姗山东师范大学化学化工与材料科学学院山东济南250014

学习轨迹是特定教学活动下学生学习心理的轨迹,有利于教师掌握学情从而实现以学生为中心的教学。在国内外相关文献基础上,首先明确学习轨迹是学生在由教学引起的关键事件上认知、情感、动机等与学习有关的动态心理过程的总和;提炼出学... 详细信息

学习轨迹是特定教学活动下学生学习心理的轨迹,有利于教师掌握学情从而实现以学生为中心的教学。在国内外相关文献基础上,首先明确学习轨迹是学生在由教学引起的关键事件上认知、情感、动机等与学习有关的动态心理过程的总和;提炼出学习轨迹的3个特征,分别为特异性、序列性和结构化;之后介绍了学习轨迹的探查、教学与测评以及教师专业发展等研究领域;最后从教学设计和有效教学2个方面探讨了学习轨迹的研究价值,为化学教育研究者了解该领域提供一些参考。

关键词：学习轨迹学习进阶教师专业化

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Ten key issues for ecological restoration of territorial space

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National Science Review 2024年第7期11卷 75-79页

作者： Jian Peng Dongmei Xu Zihan Xu Hui Tang Hong Jiang Jianquan Dong Yanxu liu Technology Innovation Center for Integrated Ecosystem Restoration and Sustainable Utilization Ministry of Natural Resources College of Urban and Environmental Sciences Peking University School of Soil and Water Conservation Beijing Forestry University Key Laboratory for Environmental and Urban Sciences School of Urban Planning and DesignShenzhen Graduate School Peking University School of Landscape Architecture Beijing Forestry University State Key Laboratory of Earth Surface Processes and Resource Ecology Faculty of Geographical Science Beijing Normal University

Ecological restoration is an effective measure to protect biodiversity, sustain ecosystem services and enhance socialecological resilience. It is defined as the process of assisting the recovery of an ecosystem that h... 详细信息

关键词： PRIORITY AREAS

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小柴胡颗粒的化学成分及大鼠口服后的入血成分分析

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中国中药杂志 2024年第15期49卷 4078-4090页

作者：刘晓丽欧沛思林爱华刘奕明广州中医药大学第二临床医学院广东广州510006 广州中医药大学第二附属医院珠海医院广东珠海519000 广东省中医证候临床研究重点实验室广东广州510120

运用超高效液相色谱-四极杆-静电场轨道阱质谱(UPLC-Q-Exactive-Orbitrap-MS)技术及超高效液相色谱串联三重四极杆质谱(UPLC-MS/MS)对小柴胡颗粒化学成分及大鼠口服后的入血成分进行鉴定。采用CORTECS UPLC C^(+)_(18)色谱柱(2.1 mm×10... 详细信息

运用超高效液相色谱-四极杆-静电场轨道阱质谱(UPLC-Q-Exactive-Orbitrap-MS)技术及超高效液相色谱串联三重四极杆质谱(UPLC-MS/MS)对小柴胡颗粒化学成分及大鼠口服后的入血成分进行鉴定。采用CORTECS UPLC C^(+)_(18)色谱柱(2.1 mm×100 mm,1.6μm),乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,在电喷雾离子源正、负离子模式下采集数据后与对照品比较保留时间、精确相对分子质量、二级离子碎片以及参照相关文献进行化学成分鉴别;通过皮下注射干酵母建立大鼠发热模型后,灌胃给予正常组及模型组大鼠小柴胡颗粒,给药后于不同时间点眼眶静脉取血,分离血浆,在多反应监测模式(MRM)下进行扫描鉴定入血成分。从小柴胡颗粒中共初步鉴定出112种化学成分,包括63种黄酮类、31种皂苷类、6种有机酸类、4种苯丙素类、3种氨基酸类以及5种其他类化合物;从血浆中鉴定得到18个入血原型成分。该研究鉴定了小柴胡颗粒化学成分及入血成分,为进一步的药效物质基础及质量控制研究奠定了基础。

关键词：小柴胡颗粒化学成分入血成分 UPLC-Q-Exactive-Orbitrap-MS UPLC-MS/MS

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Time-dependent model for two-phase flow in ultra-high water-cut reservoirs: Time-varying permeability and relative permeability

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Petroleum Science 2024年第4期21卷 2536-2553页

作者： Shao-Chun Wang Na Zhang Zhi-Hao Tang Xue-Fei Zou Qian Sun Wei liu College of Energy Resources Chengdu University of Technology College of Petroleum Engineering Shandong Institute of Petroleum and Chemical Technology

For the ultra-high water-cut reservoirs, after long-term water injection exploitation, the physical properties of the reservoir change and the heterogeneity of the reservoir becomes increasingly severe, which further aggravates the spatial difference of the flow field. In this study, the displacement experiments were employed to investigate the variations in core permeability, porosity, and relative permeability after a large amount of water injection. A relative permeability endpoint model was proposed by utilizing the alternating conditional expectation(ACE) transformation to describe the variation in relative permeability based on the experimental data. Based on the time dependent models for permeability and relative permeability, the traditional oil-water two-phase model was improved and discretized using the mimetic finite difference method(MFD). The two cases were launched to confirm the validation of the proposed model. The impact of time-varying physical features on reservoir production performance was studied in a real water flooding reservoir. The experimental results indicate that the overall relative permeability curve shifts to the right as water injection increases. This shift corresponds to a transition towards a more hydrophilic wettability and a decrease in residual oil saturation. The endpoint model demonstrates excellent accuracy and can be applied to time-varying simulations of reservoir physics. The impact of variations in permeability and relative permeability on the reservoir production performance yields two distinct outcomes. The time-varying permeability of the reservoir results in intensified water channeling and poor development effects. On the other hand, the time-varying relative permeability enhances the oil phase seepage capacity, facilitating oil displacement. The comprehensive time-varying behavior is the result of the combined influence of these two parameters, which closely resemble the actual conditions observed in oil field expl

关键词： Mimetic finite difference Water flooding reservoir Time-varying physical properties Numerical simulation

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Achieving a remarkable low-temperature tensile ductility in a high-strength tungsten alloy

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Tungsten 2024年第1期6卷 150-161页

作者： Xiang Cheng Zhuo-Ming Xie Xue-Feng Xie Long-Fei Zeng Rui liu Jun-Feng Yang Xue-Bang Wu Xian-Ping Wang Chang-Song liu Qiang-Feng Fang Key Laboratory of Materials Physics Institute of Solid State PhysicsHFIPSChinese Academy of SciencesHefei 230031China University of Science and Technology of China Hefei 230026China Faculty of Materials Metallurgy and Chemistry Jiangxi University of Science and TechnologyGanzhou 341000China

Hot-swaging yields a high ultimate tensile strength of 712 MPa but a limited tensile ductility with the total elongation of3.6%at a testing temperature of 200℃in a representative W-0.5wt.%ZrC *** this work,the evolution of Vickers microhardness with annealing temperatures is investigated in detail,which contributes to a rough index chart to guide the search for an optimized post-annealing *** the post-annealing around 1300℃,an outstanding tensile ductility at200℃,including a uniform elongation of 14%and a total elongation of~25%,has been achieved without the sacrifice of its *** evolution of dislocations and grain structures with the annealing temperatures accessed through backscattered scanning electron microscope and transmission electron microscope analysis reveals that the improved low-temperature tensile ductility has resulted from the reduction of residual dislocations and dislocation tanglement via the static recovery,which provides more room to accommodate dislocations,and hence stronger strain hardening ability and tensile ductility.

关键词： Tungsten Low-temperature ductility Annealing Dislocations

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A novel multi-channel CNN-LSTM and transformer-based network for diesel engine misfire diagnosis under different noise conditions

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Science China(Technological Sciences) 2024年第9期67卷 2965-2967页

作者： LI Ou LEI JunBo QIN ChengJin ZHANG ZhiNan TAO JianFeng liu ChengLiang State Key Laboratory of Mechanical System and Vibration School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Misfire fault, as a major fault of diesel engines, usually occurs because of low fuel quality, insufficient compression, ignition system failure, *** engines may experience issues such as reduced power, increased fuel consumption, and increased noise when misfire happens. Therefore, timely detection and diagnosis of diesel engine misfire faults are of great significance in the industry [1].

关键词： Diesel diagnosis network

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