检索结果-南通市图书馆

头花蓼内生真菌Gibberella intermedia抗多重耐药菌活性成分及其逆转细菌耐药性作用分析

中华中医药学会中药化学分会2.06年度学术研讨会

作者： Weidong Pan,~(a,b,d) Yongmin Zhang,~a Bixue Xu,~b Peixue Cao,~b and Guangyi liang,~(a,b,c) a Ecole Normale Superieur,Departement de Chimie,UMR CNRS 8642.2. rue Lhomond,752.1 Paris Cedex 05,France. b The Key Laboratory of Chemistry for Natural Products of Guizhou Province and {2. Academy of Sciences,Guiyang 550002.China. c Guiyang College of Traditional {2. Medicine,Guiyang 550002.China. d Guizhou University,the center for research and Development of Fine Chemicals,Guiyang,55002.,China.

Four compounds were isolated from Torricellia angulata var intermedia(Harms.) Hu (Torricelliaceae) by column chromatography accompanied synthetic structure modification method and two new polyacetylene compounds with a chiral carbon center were included whose absolute configurations were determined by the recently developed expended Mosher’s method.

关键词： polyacetylene Torricellia angulata var intermedia separation Mosher’s method configuration

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国实验方剂学杂志 2018年第7期24卷 33-37页

作者：刘俊张青艳杨斐玉徐国波周孟廖尚高贵州医科大学药学院贵州贵安新区550025 国家苗药工程技术中心贵阳550004 贵州医科大学生物与工程学院贵州贵安新区550025

目的：明确头花蓼内生真菌Gibberella intermedia抗菌活性成分，了解活性成分对临床耐药菌的逆转作用。方法：采用体外抗菌活性导向法及现代分离技术对头花蓼内生真菌***的发酵物进行活性物质分离，并利用现代MS和NMR等波谱技术鉴定其... 详细信息

目的：明确头花蓼内生真菌Gibberella intermedia抗菌活性成分，了解活性成分对临床耐药菌的逆转作用。方法：采用体外抗菌活性导向法及现代分离技术对头花蓼内生真菌***的发酵物进行活性物质分离，并利用现代MS和NMR等波谱技术鉴定其化合物结构。采用96孔板法测试单体化合物对临床耐药菌的抗菌作用以筛选出活性成分，并在无抗菌作用的浓度下评估活性成分最低抑菌浓度（MIC）的影响，了解其逆转耐药菌的作用。结果：从头花蓼内生真菌***的活性组分中分离得到6个化合物，分别为镰刀菌酸（1），吲哚-3-乙酸（2.，对羟基苯乙酸（3），原儿茶酸（4），邻羟基苯乙酸（5）和对羟基苯甲醛（6）。化合物2.6均为从真菌***代谢物中首次分离。化合物1对多重耐药大肠埃希菌、金黄色葡萄球菌和奇异变形杆菌的MIC分别为31．3，12.，62.5mg·L^-1。且化合物1具有逆转其耐药性的作用，在其1／8M[C的浓度下，能使左氧氟沙星和环丙沙星对临床耐药大肠埃希菌的MIC分别降低4倍和2.；在其1／4MIC的浓度下，能使左氧氟沙星和环丙沙星对耐药菌奇异变形杆菌的MIC分别降低了2.和4倍，且对临床耐药金黄色葡萄球菌的MIC值均降低了2.。结论：镰刀菌酸为头花蓼内生真菌***抗耐药菌主要活性成分，并对多重耐药菌大肠埃希菌、奇异变形杆菌和金黄色葡萄球菌具有逆转耐药性作用。该研究为治疗多重耐药菌尿路感染和提高喹诺酮类抗生素在尿路感染中的疗效奠定了基础。

关键词：头花蓼内生真菌次生代谢产物抗耐药菌逆转耐药性

Intrauterine Adhesions (IUAs) or Asherman’s Syndrome (AS) and the Stem Cells Treatment: A Systemic Review and Meta-Analysis

在线全文

学校读者我要写书评

暂无评论

Journal of Biosciences and medicines 2021年第1期9卷 105-118页

作者： Sichen Zhang ying Mao Xiaodong Zhao Huiwen Zhang Min Ma Xingbo Long Shaowei Wang Department of Gynecology and Obstetrics Beijing Hospital National Center of Gerontology Institute of Geriatric Medicine Chinese Academy of Medical Sciences Beijing China Peking Union Medical College Chinese Academy of Medical Sciences Santiao Beijing China National Cancer center/Cancer Hospital Beijing China

Background: Intrauterine Adhesions (IUAs) or Asherman’s Syndrome (AS) usually contains symptoms such as decreased menstrual flow or even amenorrhea, chronic pelvic pain, recurrent abortion and infertility. The current treatment of IUAs includes hysteroscopic adhesiolysis, oral hormone and biological barriers, but each of them has limitation. Stem cell therapy may be an expanding field seeking for therapy in IUAs. Objective: We will discuss current advances in stem cell therapy as a treatment for endometrial pathophysiology. Materials and Methods: We search on PubMed, Embase and Cochrane library and select several keywords on researches, then review the cell biology theories and animal experiments, finally do meta-analysis in human clinical trials. Results: 77 articles on PubMed, 71 articles on Embase and 17 articles on Cochrane Library, as a result, 37 articles are included under the criteria, which are intrauterine adhesions (IUAs), Asherman’s Syndrome (AS), cell therapy, stem cells, bone marrow stem cells, clinical trials, recent 10 years and human or animal experiments. The included criteria: original articles, cohort study, case control study, animal experiments, human clinical trials, high quality, 10 years recent. The excluded articles are case reports, meeting reports, low quality or more than 10 years ago. Conclusion: Stem cell may be a new therapeutic schedule for IUAs in the future clinical treatment, but it is necessary to compare it with traditional therapy such as oral hormone, also the development of random clinical tests should proceed. For clinical treatment on IUAs, stem cells could be a new choice.

关键词： Intrauterine Adhesions IUA Asherman’s Syndrome Stem Cells Therapy Infertility Meta-Analysis

Post-marketing safety monitoring of Shenqifuzheng injection: a solution made of Dangshen(Radix Codonopsis)and Huangqi(Radix Astragali Mongolici)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Traditional chinese Medicine 2014年第4期34卷 498-503页

作者： Qinghua Ai Wen Zhang Yanming Xie Wenhua Huang Hong liang Hui Cao Institute of Basic research in Clinical Medicine China Academy of Chinese Medical Sciences National Engineering research center For {2. of TCM Livzon (group) Limin Pharmaceutical Factory National Engineering research center for {2. of TCM

OBJECTIVE:Toidentify the potential risk factors associated with Shenqifuzheng injection(SFI), a solution made of Dangshen(Radix Codonopsis) and Huangqi(Radix Astragali Mongolici), for the timely provision of information to regulatory ***: A comprehensive analysis of the production process, quality standards, pharmacology,post-marketing clinical studies, and safety evaluation using the primary literature of adverse reactions(ADR), case analyses, and systematic reviews,intensive hospital safety monitoring of post-marketing drugs, and data provided by the hospital in-formationsystem(HIS).RESULTS: Sub-acute toxicity tests suggesting that a dose of 15 mL/kg(concentrated solution) had specific biological effects, whereas a smaller dose engendered no observable effects. Long-term toxicity testing in domestic rabbits showed that after SFI was administered for 90 days, the animals in each dosing group showed no chronic toxic reactions. Among 2. 100 cases observed, the incidence of an ADR was 1.85‰. From March to November 2.13,of the leading institutions and 2. sub-centers involved in the post-marketing clinical safety intensive hospital monitoring, 2. units completed 8484 cases of monitoring, and reported 2. cases of adverse reactions. No damage to renal function was found using SFI ata dosageanda treatment course larger and longer than that recommended for the adjuvant treatment of tumors. This could reduce the mortality rate of admitted patients based on the analysis of the data provided by the HIS. A total of 16 clinical case reports of adverse reactions related to SFI in 1999-2.12.were obtained through literature retrieval. These reports contained information concerning 17 cases, with adverse reaction symptoms including thrombocytopenia, rash,chills,feeling cold,palpitation,dyspnea,edemaofa lower extremity, palpebral edema, and superficial vein in flammation,among ***: This study introduces "get full access" to the flow of information on med

关键词： Post-marketing re-evaluation Safety research Shenqifuzheng injection

Two new nimbolinin-and trichilin-class limonoids isolated from the fruits of Melia azedarach

在线全文

学校读者我要写书评

暂无评论

chinese Journal of Natural medicines 2019年第3期17卷 227-230页

作者： QIU Lu HENG Li XU Rong LUO Jun LI yi Testing & Analysis center Nanjing Normal University Jiangsu Key Laboratory of Bioactive Natural Product research and State Key Laboratory of Natural medicines China Pharmaceutical University

Two new furan fragment isomerized limonoids, meliazedalides A and B(compounds 1 and 2., were isolated from the fruits of Melia azedarach Linn.. Their chemical structures were elucidated on the basis of HR-ESI-MS and 1D and 2. NMR data, which belonged to nimbolinin-and trichilin-class, respectively. Compound 2.exhibited weak inhibitory effect on NO production in lipopolysaccharide(LPS)-activated RAW 2.4.7 macrophages with IC_(50) being 37.41 μmol·L^(–1).

关键词： Melia azedarach Meliaceae Limonoid Anti-inflammatory

Bear bile powder attenuates senecionine-induced hepatic sinusoidal obstruction syndrome in mice

在线全文

学校读者我要写书评

暂无评论

chinese Journal of Natural medicines 2022年第4期20卷 270-281页

作者： JIANG Kai-Yuan ZHANG yi YE Xuan-Ling XIONG Fen CHEN Yan JIA Xia-Li ZHANG yi-Xin YANG Li XIONG Ai-Zhen WANG Zheng-Tao The MOE Key Laboratory for Standardization of chinese medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of chinese medicines Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghai 201203China Shanghai R&D center for Standardization of Traditional chinese {2. Shanghai 201203China Institute of Interdisciplinary Integrative Medicine research Shanghai University of Traditional Chinese MedicineShanghai 201203China

Hepatic sinusoidal obstruction syndrome(HSOS)via exposure to pyrrolizidine alkaloids(PAs)is with high mortality and there is no effective treatment in *** bile powder(BBP)is a famous traditional animal drug for curing a variety of hepatobiliary diseases such as cholestasis,inflammation,and ***,we aim to evaluate the protective effect of BBP against HSOS induced by senecionine,a highly hepatotoxic PA *** results showed that BBP treatment protected mice from senecionine-induced HSOS dose-dependently,which was evident by improved liver histology including reduced infiltration of inflammatory cells and collagen positive cells,alleviated intrahepatic hemorrhage and hepatic sinusoidal endothelial cells,as well as decreased conventional serum liver function *** addition,BBP treatment lowered matrix metalloproteinase 9 and pyrrole-protein adducts,two well-known markers positively associated with the severity of PA-induced *** investigation showed that BBP treatment prevents the development of liver fibrosis by decreasing transforming growth factor beta and downstream fibrotic *** treatment also alleviated senecionine-induced liver inflammation and lowered the pro-inflammatory cytokines,in which taurours-odeoxycholic acid played an important ***’s more,BBP treatment also decreased the accumulation of hydrophobic bile acids,such as cholic acid,taurocholic acid,glycocholic acid,as *** concluded that BBP attenuates senecionine-induced HSOS in mice by repairing the bile acids homeostasis,preventing liver fibrosis,and alleviating liver *** present study helps to pave the way to therapeutic approaches of the treatment of PA-induced liver injury in clinics.

关键词： Hepatic sinusoidal obstruction syndrome Pyrrolizidine alkaloids Bear bile powder Liver disease Bile acids

Effect of Banxia Xiexin decoction on Helicobacter pylori-related peptic ulcers and its possible mechanism via the TGF-β/Smad signaling pathway

在线全文

学校读者我要写书评

暂无评论

Journal of Traditional chinese Medicine 2018年第3期38卷 419-426页

作者： Chen Shaofang liang Huiqing Chen Shaodong Huang Yanping Wan Shichuan Huang Yanxia Department of Clinical Basis of Traditional chinese Medicine College of Chinese Medicine Fujian University of Traditional Chinese Medicine Liver Disease center Xiamen Hospital of Traditional Chinese Medicine Department of Traditional chinese Medicine Medical College of Xiamen University

OBJECTIVE: To investigate the effect of Banxia Xiexin decoction(BXD) on Helicobacter pylori(Hp)-related peptic ulcers(PUs) and the possible mecha-nism underlying BXD actions via the transforming growth factor-β/small mothers against decapentaplegic(TGF-β/Smad) signaling ***: PU patients with cold-heat complex syndrome were randomly assigned to groups that received chinese or Western {2. with 2. patients in each group. Serum was collected after 7 d of treatment. The healthy group included 2. individuals. Gastric mucosal epithelial cell line GES-1 was cultured in vitro and randomly divided into the following seven groups: control, model, healthy,Western Medicine, prior treatment, low dosage,and high dosage. After 72.h of treatment with the corresponding serum, the m RNA and protein expression levels of TGF-β1, Smad3, and Smad7 were measured by reverse transcription quantitative polymerase chain reaction and western blotting, ***: The m RNA expression levels of TGF-β1 and Smad3 in GES-1 cells were increased after Hp introduction, and these increased levels were reduced by the BXD-containing serum. The protein levels of p-Smad3, but not TGF-β1 or Smad3, were significantly increased in Hp-treated GES-1 cells,and treatment with the BXD-containing serum markedly decreased the protein levels. Smad7 expression was significantly enhanced following treatment with the BXD-containing serum at transcriptional and protein levels in a dose-dependent ***: BXD regulates the TGF-β/Smad signaling pathway by inhibiting the expression of TGF-β1 and Smad3, and increasing the expression of Smad7.

关键词： Helicobacter pylori Peptic ulcer Trans-forming growth factor beta Smad proteins Banxia Xiexin decoction human drug-containing serum

Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice

在线全文

学校读者我要写书评

暂无评论

chinese Journal of Natural medicines 2023年第6期21卷 411-422页

作者： FAN Cailian WANG Guan CHEN Miao LI Yao TANG Xiyang DAI yi College of Medicine Henan Engineering Research Center of Funiu Mountain’s Medicinal Resources Utilization and Molecular MedicinePingdingshan UniversityPingdingshan 467000China Innovation center of Nursing research Nursing Key Laboratory of Sichuan ProvinceWest China HospitalSichuan University/West China School of NursingSichuan UniversityChengdu 610041China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs research College of PharmacyJinan UniversityGuangzhou 510632China

Alkaloids are a class of naturally occurring bioactive compounds that are widely distributed in various food sources and Traditional chinese *** study aimed to investigate the therapeutic effects and underlying mechanisms of alkaloid extract from Codonopsis Radix(ACR)in ameliorating hepatic lipid accumulation in a mouse model of non-alcoholic fatty liver disease(NAFLD)induced by a high-fat diet(HFD).The results revealed that ACR treatment effectively mitigated the abnormal weight gain and hepatic injury associated with ***,ACR ameliorated the dysregulated lipid metabolism in NAFLD mice,as evidenced by reductions in serum triglyceride,total cholesterol,and low-density lipoprotein levels,accompanied by a concomitant increase in the high-density lipoprotein *** treatment also demonstrated a profound anti-oxidative effect,effectively alleviating HFD-induced oxidative stress and promoting ATP *** effects were achieved through the up-regulation of the activities of mitochondrial electron transfer chain complexes Ⅰ,Ⅱ,Ⅳ,and Ⅴ,in addition to the activation of the AMPK/PGC-1α pathway,suggesting that ACR exhibits therapeutic potential in alleviating the HFD-induced dysregulation of mitochondrial energy ***,ACR administration mitigated HFD-induced endoplasmic reticulum(ER)stress and suppressed the overexpression of ubiquitin-specific protease 14(USP14)in NAFLD *** summary,the present study provides compelling evidence supporting the hepatoprotective role of ACR in alleviating lipid deposition in NAFLD by improving energy metabolism and reducing oxidative stress and ER *** findings warrant further investigation and merit the development of ACR as a potential therapeutic agent for NAFLD.

关键词： Codonopsis Radix Alkaloids Mitochondrial energy metabolism Endoplasmic Reticulum stress NAFLD

Anti-rheumatoid arthritis potential of diterpenoid fraction derived from Rhododendron molle fruits

在线全文

学校读者我要写书评

暂无评论

chinese Journal of Natural medicines 2021年第3期19卷 181-187页

作者： HE Yan-Chen YAO Yu-Min XUE Qiu-Wen FANG Xin liang Shuang Engineering research center of Modern Preparation Technology of TCM Ministry of EducationInnovation Research Institute of Traditional Chinese MedicineShanghai University of Traditional Chinese MedicineShanghai 201203China

Rhododendron molle *** is first recorded in Shengnong’s Herbal Classic,and its fruits,which are termed as Liuzhouzi,are often used to treat rheumatoid arthritis in chinese *** our ongoing investigation to develop a safer and potential new arthritis therapy,a process for the preparation of diterpenoid fraction from Rhododendron molle fruits was *** order to evaluate the main components and the anti-rheumatoid arthritis effect of the diterpenoid fraction,phytochemical and pharmacological experiments were *** the result,the main components of diterpenoid fraction were identified as rhodojaponinⅢ(1),rhodojaponinⅥ(2.,2.O-methylrhodojaponin(3),and 5′-β-D-glucopyranosy-loxyjasmonic acid(4).These four components constitute greater than 95%of diterpenoid fraction using area normalization method of *** results of CIA rat experiment showed that high dose of diterpenoid fraction(0.6 mg·kg^(-1)·d^(-1))significantly alleviated the symptoms of rheumatoid arthritis,similar to tripterygium polyglycosides,an effective RA *** mechanism studies indicated that diterpenoid fraction significantly inhibited the abnormal proliferation of T and B lymphocytes,and remarkably reduced the levels of pro-inflammatory cytokines IL-6,IL^(-1)βand TNF-α.Overall,our findings may provide a more effective and safe alternative treatment for RA using common clinical chinese {2. like tripterygium polyglycosides.

关键词： Rhododendron molle Diterpenoid fraction Anti-rheumatoid arthritis Mechanism

基于网络药理学及分子对接研究消渴清颗粒治疗糖尿病前期的作用机制

在线全文

学校读者我要写书评

暂无评论

现代药物与临床 2024年第7期39卷 1727-1738页

作者：张怡郭梦竹赵进东方朝晖安徽中医药大学第一临床医学院安徽合肥230000 安徽中医药大学第一附属医院安徽合肥230031 合肥综合性国家科学中心大健康研究院新安医学与中医药现代化研究所安徽合肥230038

目的利用网络药理学及分子对接探究消渴清颗粒治疗糖尿病前期的作用机制。方法通过中药系统药理学数据库搜索有关于消渴清颗粒中中药的有效成分与靶点,在GeneCards数据库、OMIM数据库搜索糖尿病前期的基因靶点,进而获得药物–疾病交... 详细信息

目的利用网络药理学及分子对接探究消渴清颗粒治疗糖尿病前期的作用机制。方法通过中药系统药理学数据库搜索有关于消渴清颗粒中中药的有效成分与靶点,在GeneCards数据库、OMIM数据库搜索糖尿病前期的基因靶点,进而获得药物–疾病交集靶点,将交集靶点导入String蛋白质相互作用数据库和Cytoscape构建蛋白质相互作用(PPI)网络图,并筛选核心靶点,再将药物–疾病交集靶点导入DAVID数据库,对其分别进行基因本体(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析,筛选出与疾病相关的通路,通过Cytoscape得出“通路–靶点”网络图,再通过插件分析得出“通路–靶点”网络核心靶点,取二者度值排名前7位的靶点交集作为消渴清颗粒治疗糖尿病前期的关键靶点,最后选择关键靶点与有效成分用AutoDock与Pymol软件进行分子对接及可视化。结果筛选出消渴清颗粒中药活性成分36种,其中筛选出6种关键活性成分,分别为槲皮素、山柰酚、小檗碱、β-谷甾醇、花生四烯酸、知母皂苷C,靶点基因2.2.;筛选出糖尿病前期靶点685个,交集靶点93个,关键靶点5个,分别为肿瘤坏死因子(TNF)、白细胞介素-6(IL-6)、蛋白激酶B1(Akt1)、白细胞介素-1β(IL-1β)、环加氧酶2.PTGS2.。GO通路富集主要涉及内容有炎症、药物反应,细胞生长凋亡等;KEGG通路富集分析显示糖尿病并发症中的晚期搪基化终产物及其受体(AGE-RAGE)信号通路、TNF信号通路、缺氧诱导因子-1(HIF-1)信号通路、IL-17信号通路、胰岛素抵抗通路、磷脂酰肌醇3-激酶(PI3K)/Akt信号通路等,分子对接结果显示关键靶点与有效成分具有较稳定的结合能力。结论消渴清颗粒中各种药物成分通过多靶点、多通路协同治疗糖尿病前期的作用机制,为进一步研究治疗糖尿病提供了线索。

关键词：消渴清颗粒糖尿病前期网络药理学分子对接槲皮素山柰酚小檗碱