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International Journal of Ophthalmology(English edition) 2012年第2期5卷 125-132页

作者： yi-ting xu, Hai-Feng xu School of Medicine and Life Sciences of Shandong Academy of Medical Sciences University of Jinan Jinan 250022 Shandong Province China State Key Laboratory Cultivation Base Shandong Provincial Key Laboratory of Ophthalmology Shandong Eye Institute Shandong Academy of Medical Sciences Qingdao 266071 Shandong Province China

AIM: Age-related macular degeneration (AMD) is a multifactorial disease and a prevalent cause of visual impairment in developed countries. Many studies suggest that age-related maculopathy susceptibility 2 (ARMS2) is a second major susceptibility gene for AMD. At present, there is no functional information on this gene. Therefore, the purpose of the present study was to detect the expression of ARMS2 in retinal pigment epithelium (RPE) cells and to investigate the effect of ARMS2 on the phagocytosis function of RPE cells. METHODS: Immunofluorescence and reverse transcriptase PCR were used to demonstrate the presence and location of ARMS2 in ARPE-19 (human retinal pigment epithelial cell line, ATCC, catalog ***-2302) cells. siRNA was used to knock down ARMS2 mRNA, and the effects of the knockdown on the phagocytosis function of the ARPE-19 cells were evaluated via Fluorescence Activated Cell Sorting (FACS). RESULTS: ARMS2 was present in ARPE-19 cells, localized in the cytosol of the perinuclear region. The expression of ARMS2 mRNA (messenger RNA) in ARPE-19 cells transfected with ARMS2-siRNA (small interfering RNA, 0.73+/- 0.08) was decreased compared with normal cells (1.00+/- 0.00) or with cells transfected with scrambled siRNA (0.95+/- 0.13) (P<0.05). After incubation of RPE cells with a latex beads medium for 12, 18, or 24 hours, the fluorescence intensities were 38.04 +/- 1.02, 68.92 +/- 0.92, and 78.00 +/- 0.12 in the ARMS2-siRNA-transfected groups, respectively, and 77.98 +/- 5.43, 94.87 +/- 0.60, and 98.30 +/- 0.11 in the scrambled siRNA-transfected groups, respectively. The fluorescent intensities of the same time points in the two groups were compared using Student's t-test, and the p values were all less than 0.001 at the three different time points. CONCLUSION: There is endogenous expression of ARMS2 in ARPE-19 cells. ARMS2 plays a role in the phagocytosis function of RPE cells, and this role may be one of the mechanisms that participates in the develo

关键词： age-related maculopathy susceptibility 2 age-related macular degeneration retinal pigment epithelium phagocytosis

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Experimental analysis of matrix moveable oil saturation in tight sandstone reservoirs of the south Ordos Basin,China

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Energy Geoscience 2024年第1期5卷 184-195页

作者： ting xu Jun Pu xuejie Qin yi Wei Science and Technology Development Department Petroleum Exploration&Production Research InstituteSINOPECBeijing100083China Center Research Department of North China Petroleum Exploration&Production Research Institute SINOPECBeijing100083China Oil Field Development Department Petroleum Exploration&Production Research InstituteSINOPECBeijing100083China

Tight oil reservoirs in the south Ordos Basin are characterized by fractured,heterogeneous oil-bearing strata(an oil saturation of less than 55%on average),normal pressure(0.8±)and extra-low permeability(less than 0.3 mD).In the Chang 8 tight sandstone reservoir in Honghe oilfield,micro-and nanopores,especially those with a pore-throat radius of less than 1 mm,account for more than 90%.Fluid flow in the matrix is non-linear and crude oil flow rates are very low under normal pressure *** improved understanding of oil mobility in a tight matrix is key to further development of normalpressure tight-oil resources in the continental *** this study,constant-velocity and high-pressure mercury injection experiments were conducted using samples of typical tight sandstone cores obtained from the south of Ordos Basin.A new method for reconstructing the full-scale pore-throat distribution characteristics of tight sandstone reservoirs was established successfully,based on which multistage centrifugal tests,tests of low-pressure differential displacement of oil by water,and nuclear magnetic resonance tests were conducted in order to obtain the distribution characteristics of moveable fluid in different *** moveable oil saturation(MOS)and degree of oil recovery(*** of accumulative oil production to producing geologic reserves)of the core samples under different differential pressures for displacement were *** for the tight oil reservoirs in the south Ordos Basin,the moveable fluids are mainly stored in sub-micron(0.10-0.5 mm)*** Type I reservoirs(k>0.1 mD),the volume percentage of moveable fluid in pores with a radius larger than 0.5 mm is relatively high(greater than 40%).The degree of oil recovery of water flooding serves as the basis for forecasting recoverable reserves for tight oil *** reserves under water flooding,mainly occur in pores with a radius greater than 0.5 *** contribution of Type I reserves to oil pro

关键词： Tight reservoir Pore-throat structure Moveable fluid volume Moveable oil saturation(MOS) Waterflooding oil recovery South Ordos basin

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Movement of ACC oxidase 3 mRNA from seeds to flesh promotes fruit ripening in apple

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Molecular Plant 2024年第8期17卷 1221-1235页

作者： ting Wang yi Zheng Chen xu Yulin Deng Xinyi Hao Zicheng Chu Ji Tian yi Wang Xinzhong Zhang Zhenhai Han ting Wu College of Horticulture China Agricultural UniversityBeijingChina Plant Science and Technology College Bioinformatics CenterBeijing University of AgricultureBeijingChina

Xenia,the phenomenon in which the pollen genotype directly affects the phenotypic characteristics of maternal tissues(i.e.,fruit ripening),has applications in crop production and ***,the underlying molecular mechanism has yet to be ***,we investigated whether mobile mRNAs from the pollen affect the ripening and quality-related characteristics of the fruit using cross-pollination between distinct Malus domestica(apple)*** demonstrated that hundreds of mobile mRNAs originating fromthe seeds are deliveredto *** themovementof one of these mRNAs,AcC oxidase 3(MdAco3),is coordinated with fruit *** acid treatment,which can cause plasmodesmal closure,blocks MdAcO3 movement,indicating that MdAcO3 transcripts may move through the *** assess the role of mobile MdAcO3 transcripts in apple fruit,we created MdACO3-GFP-expressing apple seeds using MdACO3-GFP-overexpressing pollen for pollination and showed that MdACO3 transcripts in the transgenic seeds move to the flesh,where they promote fruit ***,we demonstrated that MdAco3 can be transported from the seeds to fruit in the fleshy-fruited species tomato and *** results underscore the potential of mobile mRNAs from seeds to influence fruit characteristics,providing an explanation for the xenia ***,our findings highlight the feasibility of leveraging diverse pollen genomic resources,without resorting to genome editing,to improve fruitquality.

关键词： fruit ripening xenia ACO3 mobile RNA seed(to flesh)

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How to build a habitable planet:an interview with Charles H. Langmuir

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National Science Review 2024年第6期11卷 76-80页

作者： yi-Gang xu the Guangzhou Institute of Geochemistry Chinese Academy of Sciences

Earth is the only known habitable planet in the solar system. Understanding how Earth developed its unique habitability has been the frontier of Earth sciences and has become one of the main themes of current deep-space explorations. What are the decisive factors that led to a habitable planet? What is the role of solid Earth processes in the origin of life and in modulating the surface environment? Are Earth’s habitability studies relevant to current challenges that human beings face? These questions have attracted the interest of both scientists and the public alike.

关键词： Earth (planet)

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A 117-W 1.66-Times Diffraction Limited Continuous-Wave Nd:YVO4 Zigzag Slab Laser with Multilayer Amplified-Spontaneous-Emission Absorbing Coatings

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Chinese Physics Letters 2020年第6期37卷 31-34页

作者： Zhi-Feng Zhang Shuai Li Yang Li Yang Kou Ke Liu Yan-Yong Lin Lei Yuan yi-ting xu Qin-Jun Peng Zu-Yan xu University of Chinese Academy of Sciences Beijing 100190China Key Laboratory of Solid-State Laser Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China Beijing Aerocim Technology Co.Ltd Beijing 100043China

We report a continuous-wave end-pumped Nd:YVη4 zigzag slab laser with multilayer amplified spontaneous emission(ASE)absorbing *** coatings are deposited on the slab faces.A five-layer structure consists of SiO2-Ti-SiO2-Ti-Au,and the thicknesses are 2520 nm,10 nm,160 nm,24 nm and 200 nm,*** designed coatings show good performance for the ASE control in the experimental tests.A stable-unstable hybrid laser oscillator along orthogonal directions in the slab aperture is further configured,achieving the 117 W output at a pump of 328 *** beam quality factors M2 in the unstable direction and stable direction are 1.57 and1.66,respectively.

关键词： pumped pump unstable

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Exhaustive review of acceleration strategies for Monte Carlo simulations in photon transit

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Journal of Innovative Optical Health Sciences 2024年第5期17卷 5-21页

作者： Louzhe xu Zijie Zhu ting Li Institute of Biomedical Engineering Chinese Academy of Medical Sciences&Peking Union Medical College Tianjin 300192 P.R.China

Monte Carlo simulation techniques have become the quintessence and a pivotal nexus of inquiry in the realm of simulating photon movement within biological *** the stochastic sampling of tissue archetypes delineated by explicit optical characteristics,Monte Carlo simulations possess the theoretical capacity to render unparalleled accuracy in the depiction of exceedingly intricate ***,the quintessential challenge associated with Monte Carlo simulation methodologies resides in their extended computational duration,which significantly impedes the re¯nement of their ***,this discourse is speci¯cally dedicated to exploring innovations in strategies and technologies aimed at expediting Monte Carlo *** delves into the foundational concepts of various acceleration tactics,evaluates these strategies concerning their speed,accuracy,and practicality,and amalgamates a comprehensive overview and critique of acceleration methodologies for Monte Carlo ***,the discourse envisages prospective trajectories for the employment of Monte Carlo techniques within the domain of tissue optics.

关键词： Medical optics photon transport simulation Monte Carlo simulation acceleration

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Characteristics of long-distance mobile mRNAs from shoot to root in grafted plant species

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Horticultural Plant Journal 2024年第1期10卷 25-37页

作者： Mengmeng Fu Zhen xu Huaying Ma yifan Hao Ji Tian yi Wang Xinzhong Zhang xuefeng xu Zhenhai Han ting Wu College of Horticulture China Agricultural UniversityBeijing 100193China Plant Science and Technology College Beijing University of AgricultureBeijing 102206China

Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing ***,the long-distance delivery of RNAs was proved to involve multiple ***,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RBP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus *** several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were *** non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance *** addition,we found that RBPs were conserved among herbaceous and woody plants as well as related *** paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs.

关键词： Grafting combination Long-distance mobile mRNA TLS RNA binding proteins

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A stable staggered-grid finite-difference scheme for acoustic modeling beyond conventional stability limit

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Petroleum Science 2024年第1期21卷 182-194页

作者： Jing-yi xu Yang Liu National Key Laboratory of Petroleum Resources and Engineering China University of Petroleum(Beijing)Beijing 102249China China University of Petroleum(Beijing) Karamay CampusKaramay 834000XinjiangChina

Staggered-grid finite-difference(SGFD)schemes have been widely used in acoustic wave modeling for geophysical *** improved methods are proposed to enhance the accuracy of numerical ***,these methods are inevitably limited by the maximum Courant-Friedrichs-Lewy(CFL)numbers,making them unstable when modeling with large time sampling intervals or small grid *** solve this problem,we extend a stable SGFD scheme by controlling SGFD dispersion relations and maximizing the maximum CFL ***,to improve modeling stability,we minimize the error between the FD dispersion relation and the exact relation in the given wave-number region,and make the FD dispersion approach a given function outside the given wave-number area,thus breaking the conventional limits of the maximum CFL ***,to obtain high modeling accuracy,we use the SGFD scheme based on the Remez algorithm to compute the FD *** addition,the hybrid absorbing boundary condition is adopted to suppress boundary reflections and we find a suitable weighting coefficient for the proposed *** derivation and numerical modeling demonstrate that the proposed scheme can maintain high accuracy in the modeling process and the value of the maximum CFL number of the proposed scheme can exceed that of the conventional SGFD scheme when adopting a small maximum effective wavenumber,indicating that the proposed scheme improves stability during the modeling.

关键词： Acoustic wave Staggered-grid finite-difference(SGFD) modeling Courant-friedrichs-lewy(CFL)number Stability

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High-power ultraviolet 278-nm laser from fourth-harmonic generation of an Nd：YAG amplifier in CsB3O5 crystal

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Chinese Physics B 2018年第5期27卷 265-269页

作者： Miao He Feng Yang Cheng Dong Zhi-Chao Wang Lei Yuan yi-ting xu Guo-Chun Zhang Zhi-Min Wang Yong Bo Qin-Jun Peng Da-Fu Cui yi-Cheng Wu Zu-Yan xu Key Laboratory of Solidstate Laser Technical Institute of Physics and Chemistry (TIPC) Chinese Academy of Sciences Beijing 100190 China Key Laboratory of Functional Crystal and Laser Technology TIPC Chinese Academy of Sciences Beijing 100190 China University of Chinese Academy of Sciences Beijing 100049 China

We report on the experimental investigation and theoretical analysis of a nanosecond pulse high power ultraviolet（UV） 278 nm laser by fourth-harmonic generation（FHG） of a 1112-nm Nd：YAG amplifier in LiB3O5（LBO） and CsB3O5（CBO） crystals. The UV laser delivers a maximum average power of 10.3 W at 278 nm with peak power of 36.8 k W under input pump power of 41 W at 556 nm. This is, to the best of our knowledge, the highest output power at the specific UV wavelength of 278 nm. We also performed the theoretical investigation on the FHG with a model in the Gaussian approximation of both spatial and temporal profiles, especially accounting for the two-photon absorption effect in CBO crystal for the first time. The average output power, pulse width, and beam spatial distribution of the UV laser were simulated. The theoretical calculations are in close agreement with the experimental results.

关键词： ultraviolet lasers frequency doubled CsB3O5(CBO)crystal two-photon absorption

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Volatiles in the mantle transition zone and their effects on big mantle wedge systems

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National Science Review 2024年第06期11卷 15-18页

作者： Yu Wang yi-Gang xu State Key Laboratory of Isotope Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)

The mantle transition zone (MTZ), located between the upper and lower mantle, is characterized by sudden seismicvelocity discontinuities at depths of 410 and 660 km where significant change in mineralogical composition occurs. As a link between the upper and lower mantle,the MTZ is a layer of special significance in Earth’s deep structure, and a critical interface in geodynamics and material circulation.

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