检索结果-南通市图书馆

集成电路应用 2024年第7期41卷 314-315页

作者：解群国网江苏省电力有限公司建湖供电分公司江苏224700

阐述智能变电站继电保护运维防误的关键技术,包括自适应继电保护技术、高精度同步测量技术。探讨智能变电站继电保护运维防误中的可视化流程、智能诊断、动态权限管理措施。

关键词：继电保护运维防误技术可视化流程

Isolation and Experimental Study on Bacteriostasis of Bacillus haynesii

在线全文

学校读者我要写书评

暂无评论

Journal of Clinical and Nursing Research 2024年第7期8卷 219-227页

作者： Jiedan Liao Mengbo Yu Jiaming Li Zhiheng xie Wanjun Gu Shuzhen Zhou yi Ma College of Animal Science and Technology Foshan UniversityFoshan 528225Guangdong ProvinceChina Foshan City Nanhai Eastern Along Pharmaceutical Co. LtdFoshan 528234Guangdong ProvinceChina Department of Veterinary Medicine College of Coastal Agricultural SciencesGuangdong Ocean UniversityZhanjiang 524088Guangdong ProvinceChina

This experiment aims to isolate and inhibit three bacteria strains to provide candidate strains for the development and application of *** bacterial morphological identification,16S rDNA sequence alignment,and genetic evolution analysis,three strains were identified as Bacillus haynesii,named HP01,HD02,and *** biosurfactant activity tests,C-TAB tests,hemolysis tests,and antibacterial activity analyses,the results showed that all three strains of *** exhibited significant biosurfactant ***,the solutions of the three strains demonstrated a pronounced antibacterial effect on Staphylococcus *** resistance and safety of commonly used drugs were evaluated using the tablet diffusion method and a mouse feeding *** results indicated that the three strains were not resistant to commonly used antibacterial drugs,and the oral bacterial solution was not pathogenic and had high safety in *** study concluded that all three *** strains met the basic conditions for use,with *** HP01 being the most promising candidate.

关键词： Bacillus haynesii Separation identification Antibacterial activity Safety

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

工业燃机轴流压气机级间引气结构的压损特性

中国电机工程学报 2024年第11期44卷 4395-4405,I0018页

作者：丁骏戴斌唐燕佳谢健郑群蓝吉兵杭州汽轮控股有限公司浙江省杭州市310022 杭州汽轮动力集团股份有限公司浙江省杭州市310022 杭州汽轮新能源有限公司浙江省杭州市310022 哈尔滨工程大学动力与能源工程学院黑龙江省哈尔滨市150001

为研究级间引气结构的压损特性,为工业燃机中的相关结构设计提供参考,采用仿真手段对真实的工业燃气轮机多级轴流压气机的级间引气结构进行数值研究,计算域涵盖压气机的多个主流通道和旁路的引气槽道、腔室、管路。结果表明:小引气率下... 详细信息

为研究级间引气结构的压损特性,为工业燃机中的相关结构设计提供参考,采用仿真手段对真实的工业燃气轮机多级轴流压气机的级间引气结构进行数值研究,计算域涵盖压气机的多个主流通道和旁路的引气槽道、腔室、管路。结果表明:小引气率下,引气口的气流滞止和折转所导致的压力损失占总损失的五成以上;大引气率下,引气腔室内的流动掺混和引气支管口的流动分离所导致的压力损失占总损失的七成以上。不同区域的流动损失受引气率、引气管路的雷诺数、引气腔室的水力直径影响。下一步可以通过神经网络的方法训练得到零维的级间引气模型,用于进一步的性能评估。

关键词：工业燃机轴流压气机引气数值仿真缩放仿真

维普期刊数据库博看期刊

构建高效稳定的低温反向偏置双极膜电解槽用于二氧化碳还原

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Catalysis 2024年第4期59卷 82-96页

作者：谢逸徐湛友卢千王莹香港中文大学化学系中国香港新界江苏省大气环境与装备技术协同创新中心江苏省大气环境监测与污染控制高技术研究重点实验室UNIST-NUIST中韩能源与环境联合实验室南京信息工程大学环境科学与工程学院江苏南京210044

二氧化碳电化学还原为高附加值的化学品和原料是一种具有应用前景的负碳技术.在过去十几年里,研究人员在碱性和中性电解质中进行了大量的二氧化碳电还原研究,并在活性、选择性和稳定性方面取得了很多进展.然而,二氧化碳与碱性电解液反... 详细信息

二氧化碳电化学还原为高附加值的化学品和原料是一种具有应用前景的负碳技术.在过去十几年里,研究人员在碱性和中性电解质中进行了大量的二氧化碳电还原研究,并在活性、选择性和稳定性方面取得了很多进展.然而,二氧化碳与碱性电解液反应生成碳酸盐,导致碳利用率较低(远低于产业化应用要求).因此,未来二氧化碳电还原领域面临的关键挑战是如何有效提高碳利用率.为解决这一挑战,在酸性电解质中进行二氧化碳电还原成为了一个可行的方案,它可以有效避免“碳酸盐问题”的发生.而基于双极膜的二氧化碳电解槽技术,则允许阴/阳极在不同pH的电解质中运行,是提高碳利用率的有效方案之一.本文首先介绍了双极膜的运行模式、热力学过程、物质传输现象、水解离/合成过程以及极限电流密度的起源,并总结了近期在双极膜优化方面取得的进展.然后,进一步聚焦于双极膜在常用的三类高效二氧化碳电解槽(流通池电解槽、膜电极电解槽和固态电解质电解槽)中的应用,详细探讨了其研究进展及所面临的挑战,并强调了针对不同结构电解槽的优化策略.值得一提的是,基于反向偏置双极膜的电解槽设计在促进质子传输至阴极、抑制碳酸盐形成方面表现出显著优势,进而有效提高了碳利用率.然而,大的质子通量同时也促进了竞争性的析氢反应,这在一定程度上抑制了二氧化碳转化为多碳产物.为了解决这一问题,流通池电解槽中的阴极电解液可以被用作缓冲层,通过调节阴极液的组成和厚度,实现电极表面在较高的pH下进行二氧化碳电还原,同时本体电解液维持在酸性,从而促进生成的碳酸盐再生为二氧化碳.另一方面,膜电极电解槽,又称零间隙电解槽,其设计特点在于催化剂层和膜直接接触,这种结构降低了电解液引起的电压损耗,提高了电解效率.然而,这种紧密接触也导致阴极表面的化学环境受到膜的强烈影响.大量质子通过膜传输至阴极,导致催化剂处于强酸性环境中,这为开发高效且稳定的二氧化碳还原电催化剂带来了很大挑战.近期,多种策略已成功应用于设计能够在pH≤1电解质中稳定运行的二氧化碳还原催化剂,包括双金属催化剂、杂原子掺杂金属催化剂和先进分子催化剂.此外,也可以通过构建界面缓冲层来精细调节局部pH值并优化阴极表面化学环境,从而进一步提升膜电极电解槽中二氧化碳电还原的选择性.为了实现商业应用,二氧化碳电解槽需要直接生成高浓度的产品,以减少后续的分离成本.为此,固态电解质二氧化碳电解槽提供了一种有效的解决方案.通过在阴离子交换膜和阳离子交换膜之间引入固态电解质,使得阴极表面形成的带负电物质(例如CH3COO-)能够顺利通过阴离子交换层并输送到固态电解质中,随后与阳极提供的质子结合,形成高纯度液体产品.最后,本文提出了双极膜二氧化碳电解槽面临的机遇和挑战,其中包括:(1)开发具有高离子选择性、高机械稳定性和低膜阻的新型双极膜;(2)开发对高附加值产品具有高本征活性和选择性的新型催化剂;(3)构筑具有高效气/液/电子传输的气体扩散电极;(4)设计低能耗和高稳定性的电解槽.综上所述,本文详细介绍了基于反向偏置双极膜的二氧化碳电解槽在研究进展以及工业应用方面的可行性,为未来开发具有高碳利用率和能量效率的二氧化碳还原技术提供了参考.

关键词：二氧化碳还原电催化双极膜碳利用率能量效率

用于生物分离的石墨烯表面可控释适配体支架的设计及实验验证

在线全文

学校读者我要写书评

暂无评论

中国生物化学与分子生物学报 2024年第8期40卷 1132-1143页

作者：潘逸群徐瑗聪王群王明连北京工业大学化学与生命科学学院607 北京100020 北京工业大学材料科学与工程学院307 北京100020

磁分离是从生物样本中富集特定目标物的常用方法。传统特异性磁分离方法一般通过将抗体与磁珠偶联获得免疫磁珠的方式用于特定目标物的捕获,但由于抗体有成本高、捕获目标物后不易释放等缺点,免疫磁珠难以满足众多研究领域对同时满足目... 详细信息

磁分离是从生物样本中富集特定目标物的常用方法。传统特异性磁分离方法一般通过将抗体与磁珠偶联获得免疫磁珠的方式用于特定目标物的捕获,但由于抗体有成本高、捕获目标物后不易释放等缺点,免疫磁珠难以满足众多研究领域对同时满足目标物大批量、高特异性富集的需求。核酸适配体同抗体一样具备目标物高特异性捕获的能力,相较抗体具有制备成本低、结构简单和化学稳定性高等优点,更适合用来大批量、高特异性富集特定目标物,因此在本研究中,本文利用石墨烯对单链和双链核酸的吸附能力不同的特性,以CD63适配体为例设计了一套可以将适配体展示于石墨烯表面并用于捕获、富集和释放目标物的方法。该设计主要包括2个单元,一是可以贴附于石墨烯表面并将CD63适配体展示于石墨烯外的双链寡核苷酸支架,二是采用与支架足部序列互补的单链寡核苷酸,将所捕获的目标物从石墨烯表面解吸附。本研究首先以氧化石墨烯(GO)纸为石墨烯界面,通过对支架或CD63蛋白等进行荧光标记,并对比支架释放前后及CD63蛋白捕获前后GO纸表面荧光强度变化表示其支架释放效果与CD63蛋白捕释情况,随后应用氨基修饰的石墨烯壳铁氮磁珠搭载CD63适配体双链支架,用于捕获细胞裂解液中CD63蛋白。结果表明,该支架可以将适配体展示于石墨烯表面捕获CD63蛋白并可以可控释放,使用挑选的改性石墨烯磁珠搭载该适配体双链支架,可以用于细胞裂解液中CD63蛋白的捕获。该方法有望实现多种蛋白质的特异性富集或通过捕获外泌体膜蛋白而富集外泌体等多种用途。

关键词：生物分离磁分离适配体石墨烯

同方期刊数据库

Extracellular vesicles from human urine-derived stem cells delay aging through the transfer of PLAU and TIMP1

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第3期14卷 1166-1186页

作者： Shanshan Rao Zehui He Zun Wang Hao yin Xiongke Hu yijuan Tan Tengfei Wan Hao Zhu yi Luo Xin Wang Hongming Li Zhenxing Wang Xinyue Hu Chungu Hong yiyi Wang Mingjie Luo Wei Du Yuxuan Qian Siyuan Tang Hui xie Chunyuan Chen Department of Orthopedics Movement System Injury and Repair Research CenterXiangya HospitalCentral South UniversityChangsha 410008China Hunan Key Laboratory of Angmedicine Changsha 410008China Xiangya School of Nursing Central South UniversityChangsha 410013China Department of Pediatric Orthopedics Hunan Children’s HospitalUniversity of South ChinaChangsha 410007China Department of Respiratory Medicine Xiangya HospitalCentral South UniversityChangsha 410008China School of Nursing Xinjiang Medical UniversityUrumqiXinjiang 830000China Department of Rehabilitation Xiangya HospitalCentral South UniversityChangsha 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008China

Aging increases the risks of various diseases and the vulnerability to *** senescence is a hallmark of aging that contributes greatly to aging and aging-related *** study demonstrates that extracellular vesicles from human urine-derived stem cells(USC-EVs)efficiently inhibit cellular senescence in vitro and in *** intravenous injection of USC-EVs improves cognitive function,increases physical fitness and bone quality,and alleviates aging-related structural changes in different organs of senescence-accelerated mice and natural aging *** anti-aging effects of USC-EVs are not obviously affected by the USC donors’ages,genders,or health *** analysis reveals that USC-EVs are enriched with plasminogen activator urokinase(PLAU)and tissue inhibitor of metalloproteinases 1(TIMP1).These two proteins contribute importantly to the anti-senescent effects of USC-EVs associated with the inhibition of matrix metalloproteinases,cyclin-dependent kinase inhibitor 2A(P16INK4a),and cyclin-dependent kinase inhibitor 1A(P21cip1).These findings suggest a great potential of autologous USC-EVs as a promising anti-aging agent by transferring PLAU and TIMP1 proteins.

关键词： Extracellular vesicles Urine-derived stem cells Cellular senescence Anti-aging PLAU TIMP1 Senescence-accelerated mice Natural aging mice

Non-invasive estimation of pulmonary hypertension and clinical deterioration risk in pediatric congenital heart disease:Development and validation of predictive tools

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2024年第11期137卷 1384-1386页

作者： Ting Wang Dansha Zhou Yuqin Chen Suhua Kuang Yue Xing Qijian yi Zhengxia Pan Weibin Xu Jiao Rao Yunqi Liu Guoliang Lu Ziying Lin Xiang Li yi xie Yulong Wu Peng An Xiaoxiao Deng Jiayue He Jiayi xie Chenxi Li Gang Geng Daiyin Tian Enmei Liu Jingsi Huang Zhou Fu Jian Wang Department of Respiratory Chongqing Higher Institution Engineering Research Center of Children’s Medical Big Data Intelligent ApplicationNational Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China State Key Laboratory of Respiratory Diseases National Center for Respiratory MedicineGuangdong Key Laboratory of Vascular DiseasesNational Clinical Research Center for Respiratory DiseasesGuangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouGuangdong 510120China Department of Cardiac Surgery The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouGuangdong 510120China Department of Cardiovascular Medicine Children’s Hospital of Chongqing Medical University National Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China Department of Thoracic and Cardiac Surgery Children’s Hospital of Chongqing Medical University National Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China Department of Cardiac Center of Guangdong Women and Children Hospital Guangzhou GuangzhouGuangdong 511400China Department of Guangzhou Laboratory Guangzhou International Bio IslandGuangzhouGuangdong 510005China

To the Editor:Owing to the heterogeneity of congenital heart disease-associated pulmonary hypertension(CHDPH)disease and the development of the pulmonary vascular system in pediatric patients,the management of CHD-PH in children is signicantly different from that in adults.[1]Early identication and intervention of CHD-PH are of great signicance for disease management in children with CHD,and non-invasive,accurate clinical tools are urgently needed to predict PH risk in children with ***,the applicability of the current risk-scoring system to pediatric CHD-PH remains ***,there are presently no user-friendly,non-invasive online tools available for predicting CHD-PH,and there is a scarcity of related studies in Asian *** study aimed to develop and validate the prediction tools for estimating the risk of pulmonary hypertension and clinical deterioration in children with congenital heart disease.

关键词： pediatric congenital hypertension

高频低能冲击扰动下锚固结构渐进失效试验研究

在线全文

学校读者我要写书评

暂无评论

煤炭学报 2024年第1期49卷 309-319页

作者：张农王朋阚甲广许兴亮谢正正魏群中国矿业大学矿业工程学院江苏徐州221116 中国矿业大学煤炭精细勘探与智能开发全国重点实验室江苏徐州221116 徐州工程学院土木工程学院江苏徐州221116 江苏理工学院机械工程学院江苏常州213001

高频低能冲击扰动对巷道围岩及锚固结构造成持续疲劳损伤,是深部巷道趋向失稳的重要诱因,合理构建抗冲击动载巷道锚固支护体系及避免锚固结构失效已经成为深部煤炭开采面临的重要课题之一。采用理论分析、实验室试验、数值模拟等方法,... 详细信息

高频低能冲击扰动对巷道围岩及锚固结构造成持续疲劳损伤,是深部巷道趋向失稳的重要诱因,合理构建抗冲击动载巷道锚固支护体系及避免锚固结构失效已经成为深部煤炭开采面临的重要课题之一。采用理论分析、实验室试验、数值模拟等方法,研究了冲击载荷下锚固结构内部应力传递转化机制,阐明了锚固结构累积损伤与渐进失效机理,提出了冲击动载巷道控制准则。结果表明:压缩应力、拉伸应力与压拉快速转换形成的震荡效应是造成锚固结构损伤的三大要素;压缩、拉伸作用下介质抗压强度与不协调变形是导致锚固界面破坏的关键因素,锚固结构在内部法向驱动力作用下损伤累积,切线模量为负或单次动载冲击变形量持续逆势上扬时锚固结构失效,压缩、拉伸、震荡叠加影响下锚固结构预紧力损失及内部损伤存在明显的累积突变效应,内部裂隙以张拉裂隙为主,整体破坏从震荡效应向拉伸效应再向压缩破坏效应逐渐演化。通过提高围岩/锚固剂协调变形能力,增加锚固长度调动大范围岩体承载、保护锚固界面,保持锚固结构承载区抗剪阻滑强度大于动载冲击时内部法向驱动力,同时削弱震荡效应可有效降低锚固结构累积损伤程度。最后提出了降能-高阻-让压的冲击动载巷道控制技术准则,包括远场卸压、近场强支、破碎围岩改性、预紧力维持和让压结构,可为类似条件巷道维控提供指导。

关键词：冲击动载高频低能锚固结构累积损伤渐进失效

KLF4 facilitates chromatin accessibility remodeling in porcine early embryos

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第1期67卷 96-112页

作者： Wei Zhu Guowei Bu Ruifeng Hu Jixiang Zhang Lianyong Qiao Kai Zhou Tingting Wang Qiao Li Jingjing Zhang Linhui Wu Yali xie Taotao Hu Shichun Yang Jiaqi Guan Xiaoyu Chu Juanjuan Shi Xia Zhang Falong Lu Xin Liu yi-Liang Miao Institute of Stem Cell and Regenerative Biology College of Animal Science and Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction(Huazhong Agricultural University)Ministry of EducationWuhan 430070China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyInnovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China Frontiers Science Center for Animal Breeding and Sustainable Production Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China

Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early *** studies have illustrated several pioneer factors as being important for agricultural animals,but what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos is not *** employing low-input DNase-seq(liDNase-seq),we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation *** data revealed that KLF4 played critical roles in remodeling chromatin accessibility in porcine early *** down of KLF4 led to the reduction of chromatin accessibility in early embryos,whereas KLF4 overexpression promoted the chromatin openness in porcine ***,KLF4 deficiency resulted in mitochondrial dysfunction and developmental failure of porcine *** addition,we found that overexpression of KLF4 in blastocysts promoted lipid droplet accumulation,whereas knockdown of KLF4 disrupted this *** together,our study revealed the chromatin accessibility dynamics and identified KLF4 as a key regulator in chromatin accessibility and cellular metabolism during porcine preimplantation embryo development.

关键词： porcine embryos liDNase-seq chromatin accessibility KLF4 mitochondrion lipid droplets

大头金蝇3龄幼虫细菌多样性分析及其分离菌株分解纤维素的应用研究

在线全文

学校读者我要写书评

暂无评论

东北农业大学学报 2024年第3期55卷 52-61页

作者：王一谢桂林东北农业大学生命科学学院哈尔滨150030

Illumina高通量测序平台Novaseq,对大头金蝇(Chrysomya megacephala)3龄幼虫体表与体内细菌进行测序分析。大头金蝇3龄幼虫体表与体内贮存细菌样本中共注释396 OTUs,共注释到13门19纲49目82科137属119种细菌。体内贮存细菌的优势菌属为... 详细信息

Illumina高通量测序平台Novaseq,对大头金蝇(Chrysomya megacephala)3龄幼虫体表与体内细菌进行测序分析。大头金蝇3龄幼虫体表与体内贮存细菌样本中共注释396 OTUs,共注释到13门19纲49目82科137属119种细菌。体内贮存细菌的优势菌属为克雷伯氏菌属(Klebsiella,57.87%),通过纤维素筛选培养基培养,纯化后得到单一菌落,经鉴定为密歇根克雷伯氏菌(Klebsiella michiganensis),制成菌液后进行室内、外模拟秸秆分解试验,室内秸秆平均分解效率为87.31%,室外模拟农田中的秸秆平均分解率为88.52%,进一步对其施用于农业废弃物搭建的人工模拟土壤中,模拟土壤肥力增加,在泥土:麦麸:锯末:豆饼的比例为10:2:2:1和10:2.5:2.5:1时种子发芽指数较高,种子平均发芽指数分别达到80.25%和78.45%。结果表明,大头金蝇3龄幼虫体表携带细菌与体内贮存菌的组成结构及多样性复杂,为其分解纤维素能力及土壤改良等提供理论依据。

关键词：大头金蝇3龄幼虫体表携带细菌体内贮存菌高通量测序秸秆分解种子发芽指数