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Effects of Immunization Against Inhibin on Egg-Laying Performance in Magang and Landaise Geese

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Agricultural Sciences in China 2007年第3期6卷 355-360页

作者： HUANG Yun-mao LI Min-yi SHI Zhen-dan BAN Jin qin yi-ping College of Life Science Zhongkai University of Agriculture and Technology Guangzhou 510225 P.R.China Department of Animal Science South China Agricultural University Guangzhou 510642 P.R.China Bureau of Animal Husbandry of Tiane County Guangxi Zhuang Autonomous Region Tiane 547300 P.R.China

This study aimed to improve egg-laying performance in incubating Magang geese of Guangdong origin and Landaise geese of French origin. In experiment 1, 50 adults, egg-laying Magang geese were inoculated intramuscularly （i.m.） on days 0, 22, and 45 with 1 mL of immunogen containing 1 mg of recombinant chicken inhibin fusion protein. Immunization significantly increased blood antibody titers against inhibin fusion protein, but did not affect the egg-laying performance within 10 days after the first inoculation. From day 15, the egg-laying rate in inhibin-immunized group increased and was significantly higher than the values of control geese from day 40 to 55. However, the reverse was true from day 55 to 75 when more immunized geese developed incubation. In the entire 120 days of the experiment, the immunized geese laid 17.3 eggs in contrast to 16.4 eggs laid by the control geese. From day 30 till the end of the experiment, weight of eggs in the control geese was significantly greater than that in inhibin-immunized birds. In experiment 2, 40 Landaise geese were immunized against inhibin, as described in experiment 1. These geese laid 9.0 eggs on average in contrast to 7.3 eggs laid by nonimmunized control geese over 90 days of egg laying. The above results demonstrated that immunization against recombinant chicken inhibin fusion protein improved egg-laying performance in geese, and the increment was higher in nonincubating geese.

关键词： immunization inhibin egg-laying performance geese

PGC-1α promotes mitochondrial respiration and biogenesis during the differentiation of hiPSCs into cardiomyocytes

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Genes & Diseases 2021年第6期8卷 891-906页

作者： qin Zhou Hao Xu Liang Yan Liang Ye Xinyuan Zhang Bin Tan qin yi Jie Tian Jing Zhu Department of Pediatric Research Institute Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders(Chongqing)China International Science and Technology Cooperation Base of Child Development and Critical Disorders Children’s Hospital of Chongqing Medical UniversityChongqing400000PR China Chongqing Key Laboratory of Pediatrics Chongqing400000PR China Department of Clinical Laboratory Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders(Chongqing)China International Science and Technology Cooperation Base of Child Development and Critical Disorders Children’s Hospital of Chongqing Medical University Chongqing400000PR China Department of Cardiovascular(Internal Medicine) Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders(Chongqing)China International Science and Technology Cooperation Base of Child Development and Critical Disorders Children’s Hospital of Chongqing Medical UniversityChongqing400000PR China Chengdu Women’s and Children’s Central Hospital School of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuan Province610073PR China

Although it is widely accepted that human induced pluripotent stem cell-derived cardiomyocytes(hiPSC-CMs)are readily available,robustly reproducible,and physiologically appropriate human cells for clinical applications and research in the cardiovascular field,hiPSC-CMs cultured in vitro retain an immature metabolic phenotype that limits their application,and little is known about the underlying molecular mechanism controlling mitochondrial metabolic maturation during human induced pluripotent stem cells(hiPSCs)differentiation into *** this study,we found that peroxisome proliferator-activated receptor g coactivator-1α(PGC-1α)played an important role in inducing mitochondrial biogenesis and establishing oxidative phosphorylation(OXPHOS)during the cardiac differentiation of *** down PGC-1α by siRNA impaired mitochondrial respiration,while upregulating PGC-1α by ZLN005 promoted mitochondrial biosynthesis and function by regulating the expression of downstream genes involved in mitochondrial dynamics and oxidative metabolism in ***,we found that estrogen-related receptor a(ERRa)was required for the induction of PGC-1α stimulatory effects in *** findings provide key insights into the molecular control of mitochondrial metabolism during cardiac differentiation and may be used to generate more metabolically mature cardiomyocytes for application.

关键词： Cardiac differentiation hiPSCs Mitochondrial metabolism PGC-1α Maturation

A recurrent G367R mutation in MYOC associated with juvenile open angle glaucoma in a large Chinese family

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International Journal of Ophthalmology(English edition) 2018年第3期11卷 369-374页

作者： yi-Hua Yao Ya-qin Wang Wei-Fang Fang Liu Zhang Ju-Hua Yang yi-Hua Zhu Department of Ophthalmology the First Affiliated Hospital of Fujian Medical University Fuzhou 350005 Fujian Province China Department of Ophthalmology Taihe Hospital Shiyan 442008 Hubei Province China Hubei University of Medicine Shiyan 442008 Hubei Province China Fuzhou Eye Hospital Fuzhou 350007 Fujian Province China Biomedical Engineering Center of Fujian Medical University Fuzhou 350004 Fujian Province China

AIM： To identify the mutations of MYOC, OPTN, CYPIB1 and WDR36 in a large Chinese family affected by juvenile open angle glaucoma （JOAG）. METHODS： Of 114 members of one family were recruited in this study. Blood samples from twelve members of this pedigree were collected for further research. As a control, 100 unrelated subjects were recruited from the same hospital. The exon and flanking intron sequences of candidate genes were amplified using the polymerase chain reaction and direct DNA sequencing. RESULTS： The proband （111：10） was a seventy-three years old woman with binocular JOAG at the age of 31. A recurrent heterozygous mutation （c.1099G〉A） of MYOC was identified in the three JOAG patients and another suspect. This transition was located in the first base pair of codon 367 （GGA〉AGA） in exon 3 of MYOC and was predicted to be a missense substitution of glycine to arginine （p.G367R） in myocilin. Mutations in OPTN, CYPIB1 or WDR36 were not detected in this study. The G367R mutation was not present in unaffected family members or in 100 ethnically matched controls. Other variants of the coding regions of candidate genes were not detected in all participants. To date, this family was the largest to have been identified as carrying a certain MYOC mutation in China, further evidence of a founder effect for the G367R MYOC mutant was provided by our data. CONCLUSION： A MYOC c.1099G〉A mutation in an autosomal dominant JOAG family is identified and the characteristic phenotypes among the patients are summarized. Genetic testing could be utilized in high-risk populations and be helpful not only for genetic counseling, but also for early diagnosis and treatment of affected patients or carriers of inherited JOAG.

关键词： MYOC gene mutantion glaucoma

Bis(2-butoxyethyl) Phthalate Delays Puberty Onset by Increasing Oxidative Stress and Apoptosis in Leydig Cells in Rats

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Biomedical and Environmental Sciences 2023年第1期36卷 60-75页

作者： LIU Miao qing CHEN Hai Qiong DAI Hai Peng LI Jing Jing TIAN Fu hong WANG yi Yan CHEN Cong De LI Xiao Heng LI Jun Wei LI Zhong Rong GE Ren Shan Department of Pediatric Surgery the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhou 325027ZhejiangChina Key Laboratory of Structural Malformations in Children of Zhejiang Province Wenzhou 325000ZhejiangChina

Objective This study investigated the effects of bis(2-butoxyethyl) phthalate(BBOP) on the onset of male puberty by affecting Leydig cell development in *** Thirty 35-day-old male Sprague-Dawley rats were randomly allocated to five groups mg/kg bw per day that were gavaged for 21 days with BBOP at 0, 10, 100, 250, or 500 mg/kg bw per day. The hormone profiles;Leydig cell morphological metrics;mRNA and protein levels;oxidative stress;and AKT,mTOR, ERK1/2, and GSK3β pathways were *** BBOP at 250 and/or 500 mg/kg bw per day decreased serum testosterone, luteinizing hormone, and follicle-stimulating hormone levels mg/kg bw per day(P < 0.05). BBOP at 500 mg/kg bw per day decreased Leydig cell number mg/kg bw per day and downregulated Cyp11a1, Insl3, Hsd11b1,and Dhh in the testes, and Lhb and Fshb mRNAs in the pituitary gland(P < 0.05). The malondialdehyde content in the testis significantly increased, while Sod1 and Sod2 mRNAs were markedly downregulated, by BBOP treatment at 250–500 mg/kg bw per day(P < 0.05). Furthermore, BBOP at 500mg/kg bw per day decreased AKT1/AKT2, mTOR, and ERK1/2 phosphorylation, and GSK3β and SIRT1levels mg/kg bw per day(P < 0.05). Finally, BBOP at 100 or 500 μmol/L induced ROS and apoptosis in Leydig cells after 24 h of treatment in vitro(P < 0.05).Conclusion BBOP delays puberty onset by increasing oxidative stress and apoptosis in Leydig cells in *** graphical abstract is available on the website ***.

关键词： Bis(2-butoxyethyl)phthalate Leydig cells Testosterone Oxidative stress Gonadotropin

Emerging role of tumor cell plasticity in modifying therapeutic response

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Signal Transduction and Targeted Therapy 2020年第1期5卷 549-584页

作者： Siyuan qin Jingwen Jiang yi Lu Edouard CNice Canhua Huang Jian Zhang Weifeng He State Key Laboratory of Biotherapy and Cancer Center West China Hospitaland West China School of Basic Medical Sciences and Forensic MedicineSichuan Universityand Collaborative Innovation Center for Biotherapy610041 ChengduPeople’s Republic of China School of Medicine Southern University of Science and Technology ShenzhenShenzhenGuangdong 518055People’s Republic of China Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research ShenzhenGuangdongPeople’s Republic of China Department of Biochemistry and Molecular Biology Monash UniversityClaytonVICAustralia School of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine1166 Liutai Road611137 ChengduPeople’s Republic of China State Key Laboratory of Trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThird Military Medical University(Army Medical University)ChongqingPeople’s Republic of China Chongqing Key Laboratory for Disease Proteomics ChongqingPeople’s Republic of China

Resistance to cancer therapy is a major barrier to cancer *** views have proposed that acquisition of resistance may result from genetic ***,accumulating evidence implicates a key role of non-mutational resistance mechanisms underlying drug tolerance,the latter of which is the focus that will be discussed *** non-mutational processes are largely driven by tumor cell plasticity,which renders tumor cells insusceptible to the drug-targeted pathway,thereby facilitating the tumor cell survival and *** concept of tumor cell plasticity highlights the significance of re-activation of developmental programs that are closely correlated with epithelial–mesenchymal transition,acquisition properties of cancer stem cells,and transdifferentiation potential during drug *** observations in various cancers,this concept provides an opportunity for investigating the nature of anticancer drug *** the years,our understanding of the emerging role of phenotype switching in modifying therapeutic response has considerably *** expanded knowledge of tumor cell plasticity contributes to developing novel therapeutic strategies or combination therapy regimens using available anticancer drugs,which are likely to improve patient outcomes in clinical practice.

关键词： therapeutic plasticity thereby

Receptor variability-driven evolution of snake toxins

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Zoological Research 2018年第6期39卷 431-436页

作者： Xian-hong Ji Shang-Fei Zhang Bin Gao Shun-yi Zhu Group of Peptide Biology and Evolution State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

Three-finger toxins （TFTs） are well-recognized non- enzymatic venom proteins found in snakes. However, although TFTs exhibit accelerated evolution, the drivers of this evolution remain poorly understood. The structural complexes between long-chain α-neurotoxins, a subfamily of TFTs, and their nicotinic acetylcholine receptor targets have been determined in previous research, providing an opportunity to address such questions. In the current study, we observed several previously identified positively selected sites （PSSs） and the highly variable C-terminal loop of these toxins at the toxin/receptor interface. Of interest, analysis of the molecular adaptation of the toxin-recognition regions in the corresponding receptors provided no statistical evidence for positive selection. However, these regions accumulated abundant amino acid variations in the receptors from the prey of snakes, suggesting that accelerated substitution of TFTs could be a consequence of adaptation to these variations. To the best of our knowledge, this atypical evolution, initially discovered in scorpions, is reported in snake toxins for the first time and may be applicable for the evolution of toxins from other venomous animals.

关键词： Three-finger toxins Nicotinic acetylcholine receptor Driver

慢性内质网应激在2型糖尿病发生发展中的研究进展

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中国糖尿病杂志 2023年第2期31卷 145-148页

作者：谢燕秦虹中南大学湘雅公共卫生学院长沙410078

当内质网中合成的蛋白超过内质网折叠能承受的最大限度时,可引起慢性内质网应激(ERS)。慢性ERS在T2DM发生发展中起重要作用,主要表现为抑制Ins正常合成与分泌,降低Ins信号转导效率,扰乱Ins敏感组织代谢。本文综述ERS在T2DM发生发展中的... 详细信息

当内质网中合成的蛋白超过内质网折叠能承受的最大限度时,可引起慢性内质网应激(ERS)。慢性ERS在T2DM发生发展中起重要作用,主要表现为抑制Ins正常合成与分泌,降低Ins信号转导效率,扰乱Ins敏感组织代谢。本文综述ERS在T2DM发生发展中的研究进展。

关键词：内质网应激糖尿病,2型胰岛素抵抗糖脂代谢紊乱

A New Hemiterpene Derivative from Prinsepia utilis

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Chinese Chemical Letters 2006年第2期17卷 198-200页

作者： Jun yi HU Wei QIAO Yoshihisa TAKAISHI hong Quart DUAN The College of Pharmaceuticals and Biotechnology Tianjin University Tianjin 300072 School of Chemical Engineering Anshan university of Science ＆ Technology Anshan 114002 The School of Pharmacy Tianjin Medical University Tianjin 300070 Faculty of Pharmaceutical Sciences University of Tokushima Shomachi 1-78 Tokushima 770-8505 Japan

A new hemiterpenoid, （＋）-（2R,3S）-2-chloro-3-hydroxy-3-methyl-T-buty-rolactone was isolatexi from the leaves of Prinsepia utilis Royle. Its structure was clucidatexi by spectroscopic methods and X-ray crystallogra... 详细信息

关键词： Prinsepia utilis hemitcrpcne (+)-(2R 3S)-2-chloro-3-hydroxy-3-methyl-T-butyrolac-tone.

Seepage-Stress-Damage Coupled Model of Coal Under Geo-Stress Influence

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Computers, Materials & Continua 2018年第1期54卷 43-59页

作者： yi Xue Faning Dang Rongjian Li Liuming Fan qin Hao Lin Mu Yuanyuan Xia State Key Laboratory Base of Eco-Hydraulic Engineering in Arid Areas Xi’an University of TechnologyXi’an 710048China Institute of Geotechnical Engineering Shaanxi Provincial Key Laboratory of Loess Mechanics and EngineeringXi’an University of TechnologyXi’an 710048China Department of Civil Engineering Henan Vocational College of Water Conservancy and EnvironmentZhengzhou 450011China

In the seepage-stress-damage coupled process,the mechanical properties and seepage characteristics of coal are distinctly different between pre-peak stage and post-peak *** difference is mainly caused by damage of ***,in the process of seepage and stress analysis of coal under the influence of excavation or mining,we need to consider the weakening of mechanical properties and the development of fractures of damaged *** on this understanding,this paper analyzes the influence of damage on mechanics and seepage behavior of coal.A coupled model is established to analyze the seepage-stress-damage coupled process of *** model implemented into COMSOL and MATLAB software to realize the numerical *** examples are adopted to verify the correctness of the model and some useful conclusions are *** numerical model establishes the relationship between microcosmic damage evolution and macroscopical fracture and simulates the whole process of coal from microcosmic damage to macroscopical fracture,and the dynamic simulation of fluid flow in this *** provides a numerical tool for further research on the seepage-stress-damage analysis.

关键词： Permeability porosity gas pressure damage coupled model