检索结果-南通市图书馆

Effect of Microwave and He-Ne Laser on Enzyme Activity and Biophoton Emission of Isatis indigotica Fort

Journal of Integrative Plant Biology 2005年第7期47卷 849-855页

作者： yi-pingchen Yong-JunLIU Xun-LingWANG Zhao-YuREN MingYUE CollegeofEnvironmentSciences Xi＇anUniversityofArchitectureandTechnologyXi＇an710048China CollegeofLifeSciences NorthwestUniversityXi＇an710069China InstituteofPhotonicsandPhoto-Technology NorthwestUniversityXi＇an710069China DepartmentofBiotechnology Xi＇anUniversityofEngineeringScienceandTechnologyXi＇an710048China／／InstituteofEarthEnvironmenttheChineseAcademyofSciencesXi＇an710075China

Abstract: The seed embryos of Isatis indigotica Fort were exposed to He-Ne laser (5.23 mW/mm2, radiated for 5 min) and microwave (1.26 mW/mm2, radiated for 8 s) irradiation to determine the effects of microwave and He-Ne laser pretreatment on enzyme activities, and biophoton emission of cotyledon. Then: (i) changes in the activities of enzymes in I. indigotica cotyledon (such as amylase, transaminase, and proteinase) were measured to investigate the effects of He-Ne laser and microwave pretreatment; and (ii) biophoton emission was measured to determine the speed of cell division and metabolism. Results from these experiments indicated that: (i) the activities of amylase, transaminase, and proteinase of the cotyledon pretreated by He-Ne laser and microwave were significantly increased; and (ii) the intensity of biophoton emission was enhanced significantly by He-Ne laser and microwave irradiation. These changes suggest that He-Ne laser and microwave pretreatment can improve the inner energy of seeds, lead to an enhancement of cotyledon enzymes, and speed up the metabolism of the cell, resulting in significantly increased biophoton emission. Moreover, the mechanism of action of the effects of laser and microwave radiation on the microcalorimetric parameters, enzyme activities, and biophoton emission of seeds is discussed on the basis of the results obtained.

关键词： biophoton emission enzymes activities Isatis indigotica laser microcalorimetric effect microwave

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Association of Neutralization Sensitivity of HIV-1 Primary Isolates With Biological Properties of Isolates From HIV-1 Infected Chinese Individuals

引用

Biomedical and Environmental Sciences 2005年第2期18卷 128-136页

作者： FA-XINHEI HAI-LITANG KUN-XUEHONG JIAN-pingchen HONGPENG LINYUAN JIANG-QINGXU yi-MINGSHAO DepartmentofVirologyandImmunology NationalCenterforAIDS／STDControlandPreventionChineseCenterforDiseaseControlandPreventionBeijing100050China

Objective Although HIV-1 infection is prevalent in many regions in China, it remains largely unknown on the biological characteristics of dominant circulating isolates. This study was designed to isolate the circulating viral strains from different prevalent regions and to characterize their biological properties and neutralization sensitivity. Methods Primary viruses were isolated from fresh PBMCs using the traditional co-culture method and their capacity of inducing syncytium was tested in MT-2 cells. Meanwhile, their coreceptor usage was determined with two cell lines: Magi and GHOST (3) stably expressing CD4 and the chemokine receptor CCR5 or CXCR4. Furthermore, the sensitivity of these viruses to neutralization by HIV-1-infected patients' plasma which were highly active to neutralize SF33 strain, was quantified in GHOST cell-based neutralization assay. Results Six primary viral strains were isolated from 4 separated regions. Isolates LTG0213> LTG0214 and XVS032691 induced syncytia in MT-2 cells, and used CXCR4 as coreceptor. Isolates XJN0021, XJN0091, or SHXDC0041 did not induce syncytia, and used CCR5 as coreceptor. Overall neutralization sensitivity differed among four representative strains: HIV-1 XVS032691>LTG0214>XJN0091=SHXDC0041. Conclusion The neutralization sensitivity of HIV isolates is linked with the phenotype of isolates, in which syncytium-inducing (SI) or CXCR4-tropic (X4) viruses are more easily neutralized than non-syncytium-inducing (NSI) or CCR5-tropic (R5) viruses. The genetic subtypes based on the phylogeny of env sequences are not classical neutralization serotypes.

关键词： Human immunodeficiency virus type 1 Primary isolates Syncytium-inducing (SI) Coreceptor usage Neutralization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Community-based survey of HCV and HIV coinfection in injection drug abusers in Sichuan Province of China

引用

World Journal of Gastroenterology 2004年第11期10卷 1589-1593页

作者： Yu-HuaRuan Kun-XueHong Shi-ZhuLiu yi-XinHe FengZhou Guan-MingQin Kang-LinChen HuiXing Jian-pingchen yi-MingShao CenterforAIDS/STDControlandPrevention ChineseCenterforDiseaseControlandPreventionBei.jing100050China SichuanProvincialCenterforDiseaseControlandPrevention Chengdu610031SichuanProvinceChina XichangCenterforSTDandLeprosyControl XichangCounty615000SichuanProvinceChina

AIM: To investigate the prevalence and risk factors of HCV/HIV coinfection in injection drug abusers (IDAs) in Lianshan yi Autonomous Prefecture of Sichuan province, China. METHODS: From November 8, 2002 to November 29, 2002, a community-based survey was conducted to investigate the demographic characteristics, patterns of shared injectors devices and sexual behaviors in IDAs. Blood samples were also collected to test HCV and HIV infection. A total of 379 subjects were recruited in the study through community outreach and peer recruiting methods. RESULTS: Of the 379 IDAs, the HCV prevalence and HIV prevalence were 71.0% and 11.3%, respectively, and HCV/HIV coinfection was 11.3%. HCV infection was found in 100% and 67.3% of HIV-positive and HIV-negative IDAs, respectively. HIV prevalence was 16.0% in HCV positive IDAs while none of the HCV negative IDAs was positive for HIV. Ethnicity, shared needles or syringes and cotton in the past 3 mo and syphilis infection were associated with HCV/HIV coinfection shown by univariate analysis using chi-square test. Multivariate logistic regression analysis showed that shared needles or syringes in the past 3 mo (Odds ratio=3.121, 95% CI: 1.278-7.617, P<0.05) and syphilis infection (Odds ratio=2.914, 95% CI: 1.327-6.398, P<0.01) were significantly associated with HCV infection. No statistically significant association was found in univariate analysis between sexual behaviors and HCV/HIV coinfection. CONCLUSION: Shared needles and syringes in the past 3 mo and syphilis infection were significantly associated with HCV infection. Further sero-epidemiological prospective cohort studies should be conducted to clarify the impact of syphilis and high risk sexual behaviors on HCV transmission through unprotected sexual intercourse.

关键词：调查报告 HCV HIV 协同感染麻醉剂注射四川中国传染病

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mechanism for the Nanometer Scale Modification on HOPG Surface by Scanning Tunneling Microscope

引用

Chinese Physics Letters 1993年第9期10卷 535-538页

作者： WANG Zhonghuai DAI Changchun ZHANG pingchen HUANG Guizhen LI Renli GUO yi BAI Chunli Institute of Chemistry Acadenua.Sinica.Beijing 100080

A technique applying voltage across the tunnel junction for wnting permanent features on graphite with lines about 10nm wiidth using our home-built scanning tunneling microscope has been presented.Multiple-tip effects while modifying the surface are often met.The phenomciifi are explained in terms of the strong electric field existing at the junction between the tip an<7 the surface.In addition,the shape of the structure indented by the tip is in agreement with that of the tip.

关键词： surface. Tunnel tunneling

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Glycyrrhetinic acid-triggered cytoprotective autophagy via inositolrequiring enzyme 1α-c-Jun N-terminal kinase cascade in non-small cell lung cancer cells

引用

中国药理学与毒理学杂志 2015年第S1期29卷 81-81页

作者： Zheng-haiTANG Le-leZHANG TingLI Jia-hongLU Chung-hangLEUNG Xiu-pingchen yi-taoWANG Jin-jianLU State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical Sciences University of Macao Macao China

OBJECTIVE Glycyrrhetinic acid(GA),one of the main bioactive constituents of the famous Chinese medicinal herb Glycyrrhizauralensis Fisch,presents potent cytotoxicity to various cancer cells both in vitro and in vivo.Herein,we′d like to determine whether GA triggers autophagy in non-small cell lung cancer cells and the mechanisms involved.METHODS Cell proliferation was determined by MTT assay and colony formation.AnnexinⅤ/PI staining,Hoechst33342 staining,and Western blotting were used to detect GA-induced apoptosis.GA-induced autophagy was measured by expression of the lipid modification of light chain-3(LC3)and transfected with GFP-LC3 or GFP-RFP-LC3 plasmid.Pharmacological regulators,siRNA,and plasmid transfection were used to study the mechanisms of GA-triggered autophagy.RESULTS GA inhibited cell proliferation and induced apoptosis in a concentrationdependent manner in non-small cell lung cancer A549 cells.GA induced autophagyas evidenced by up-regulation of LC3-Ⅱexpression when combined treatment with chloroquine and induction of the red punta after GFP-RFP-LC3 plasmid transfection.Knockdown of autophagy related proteins(ATG)7,ATG 5,or beclin 1by siRNA,the expression of LC3-Ⅱand GFP-LC3 punt atriggered by GA were decreased.Furthermore,the c-Jun N-terminal kinase(JNK)pathways were activated after treatment with GA,and pretreatment with JNK inhibitor SP600125 or silence of JNK pathway by siRNA of JNK or c-jun obviously reduced GA-induced LC3-Ⅱexpression and GFP-LC3 punta formation.GA also stimulate dendoplasmic reticulum stress response by triggering inositol-requiring enzyme 1α(IRE1α)pathway,and knockdown of IRE1αinhibited the activation of JNK pathway and autophagy induced by GA.In addition,GA-induced cell proliferative inhibition and apoptosis were both enhanced when silence of autophagy as well as JNK pathway.CONCLUSION Our study demonstrated,for the first time,that GA induced a cytoprotective autophagy in non-small cell lung cancer cells by activating the IRE1α-JNK pathway,which might decreased the anti-c

关键词： glycyrrhetinic acid autophagy A549 IRE JNK

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

电针对肘关节僵硬松解术后患者Mayo功能评分和血清炎性因子的影响

引用

河北医学 2020年第11期26卷 1927-1932页

作者：朱轶欧阳元明陆平辰蒋静涵口锁堂吴耀持上海健康医学院附属第六人民医院东院针推伤科上海201306 上海交通大学附属第六人民医院针推伤科上海200233

目的:探讨电针对肘关节僵硬松解术后患者Mayo肘关节功能评分和血清炎性因子的影响。方法:对上海健康医学院附属第六人民医院东院2018年1月至2019年10月收治的300例肘关节僵硬松解术后患者进行康复,遵循随机数字表法分为对照组与观察组,... 详细信息

目的:探讨电针对肘关节僵硬松解术后患者Mayo肘关节功能评分和血清炎性因子的影响。方法:对上海健康医学院附属第六人民医院东院2018年1月至2019年10月收治的300例肘关节僵硬松解术后患者进行康复,遵循随机数字表法分为对照组与观察组,各150例,对照组给予常规康复训练,观察组在常规康复训练的基础上给予电针治疗。采用酶联免疫吸附试验(ELISA)检测治疗前后炎性因子指标[白细胞介素-6(IL-6)及白细胞介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)]水平的变化情况,采用Mayo肘关节功能评分,分析其临床疗效及不良反应情况。结果:观察组治疗有效率高于对照组(100.00%vs96.00%,χ^2=6.122,P=0.013);治疗前两组患者肘关节疼痛、活动范围、稳定性、活动能力及Mayo总分比较,差异无统计学意义(22.47±4.28 vs 22.53±4.31,t=0.121,P=0.904)、(8.96±2.15 vs 9.03±2.17,t=0.281,P=0.779)、(5.14±1.08 vs 5.09±1.01,t=0.414,P=0.679)、(17.69±4.25 vs 17.71±4.26,t=0.041,P=0.968)、(54.26±8.32 vs 54.36±8.37,t=0.104,P=0.917),治疗后,与对照组比较,观察组治疗后肘关节疼痛、活动范围、活动能力及Mayo总分等评分均高于对照组(39.96±7.28 vs 34.31±6.95,t=6.875,P<0.001)、(17.98±4.03 vs 13.86±3.52,t=9.430,P<0.001)、(25.63±4.52 vs 22.03±4.31,t=7.060,P<0.001)、(93.59±17.79 vs 80.05±16.69,t=6.798,P<0.001),但两组稳定性评分比较无差异(9.98±2.16 vs 9.85±2.07,t=0.532,P=0.595);治疗前,两组患者IL-6、IL-1、TNF-α水平比较差异无统计学意义[(11.82±2.41)ng/L vs(11.76±2.39)ng/L,t=0.217,P=0.829]、[(137.83±21.41)ng/L vs(139.47±19.52)ng/L,t=0.693,P=0.489]、[(47.83±11.10)ng/L vs(48.02±10.69)ng/L,t=0.151,P=0.880],治疗后,与对照组比较,观察组的IL-6、IL-1、TNF-α水平低于对照组[(9.52±1.03)ng/L vs(10.48±1.49)ng/L,t=6.491,P<0.001、[(60.31±12.48)ng/L vs(97.19±11.81)ng/L,t=27.281,P<0.001]、[(7.88±1.36)ng/L vs(23.84±4.65)ng/L,t=40.346,P<0.001];观察组与对照组不良反应发生率比较差异无统计学意义(2.00%vs 6.67%,χ^2=2.895,P=0.089)。结论:应用电针干预,能显著提高肘关节Mayo功能评分,降低术后炎性反应,对肘关节僵硬松解术后功能康复具有较好的临床效果,且具有较好的安全性,值得临床推广。

关键词：电针肘关节僵硬松解术 Mayo肘关节功能评分炎性因子

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：