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Neural Regeneration Research 2012年第10期7卷 761-765页

作者： Weihui Huang Dawei Zang yi Lu ping jiang Department of Neurology Tianjin First Center Hospital Tianjin Medical University Tianjin 300192 China

This study aimed to investigate the number of amino methyl isoxazole propionic acid （AMPA） receptors and production of endogenous neural stem cells in the SOD1 G93AG1H transgenic mouse model of amyotrophic lateral sclerosis, at postnatal day 60 following administration of basic fibroblast growth factor （FGF-2）. A radioligand binding assay and immunohistochemistry were used to estimate the number of AMPA receptors and endogenous neural stem cells respectively. Results showed that the number of AMPA receptors and endogenous neural stem cells in the brain stem and sensorimotor cortex were significantly increased, while motor function was significantly decreased at postnatal days 90 and 120. After administration of FGF-2 into mice, numbers of endogenous neural stem cells increased, while expression of AMPA receptors decreased, whilst motor functions were recovered. At postnatal day 120, the number of AMPA receptors was negatively correlated with the number of endogenous neural stem cells in model mice and FGF-2-treated mice. Our experimental findings indicate that FGF-2 can inhibit AMPA receptors and increase the number of endogenous neural stem cells, thus repairing neural injury in amyotrophic lateral sclerosis mice.

关键词： amino methyl isoxazole propionic acid receptor amyotrophic lateral sclerosis basic fibroblast growth factor endogenous neural stem cells

Antipyretic Effects of Eupatorium chinense and Its Mechanism

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Chinese Herbal Medicines 2015年第4期7卷 323-327页

作者： yi-ping jiang jiang-ping Xu Department of Neuropharmacology School of Pharmaceutical Sciences Southern Medical University

Objective To investigate the antipyretic effect of Eupatorium chinense and its mechanism. Methods The content of arginine vasopressin（AVP） in ventral septal area（VSA） and blood plasma, and cyclic adenosine monophosphate（c AMP） levels of hypothalamus and blood plasma were determined by enzyme linked immunosorbent assay. Results The body temperature（Tb） was decreased at 1 h after administration of E. chinense（3 and 6 g/kg） and Aspirin（0.3 g/kg） respectively, which was significantly different from the temperature of fever model group. The antipyretic effect of Aspirin and E. chinense lasted for longer time. Aspirin（0.3 g/kg） and E. chinense（3 and 6 g/kg）reduced the level of cA MP in hypothalamus of fever rats and increased AVP content in plasma. The changes in c AMP content in plasma of all drug treatment groups were not obvious. Conclusion E. chinense has strong antipyretic effect and may affect the production of AVP and c AMP in fever rats.

关键词： antipyretic arginine vasopressin cyclic adenosine monophosphate Eupatorium chinense ventral septal area

Gene cloning,protein expression,and enzymatic characterization of a double-stranded RNA degrading enzyme in Apolygus lucorum

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Insect Science 2024年第1期31卷 119-133页

作者： Jie-Yu Zhang Jing Zhao Keyan Zhu-Salzman Qin-Qin Ji yi-ping jiang Liu-Bin Xiao De-Jin Xu Guang-Chun Xu Lin-Quan Ge Yong-An Tan College of Plant Protection Yangzhou UniversityYangzhouJiangsu ProvinceChina Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjingJiangsu ProvinceChina Department of Entomology Texas A&M UniversityCollege StationTXUSA Taizhou Customs of the People's Republic of China TaizhouJiangsu ProvinceChina

RNA interference(RNAi)is a powerful tool that post-transcriptionally silences target genes in eukaryotic ***,silencing efficacy varies greatly among different insect ***,we met with little success when attempting to knock down genes in the mirid bug Apolygus lucorum via dsRNA *** disappearance of double-stranded RNA(dsRNA)could be a potential factor that restricts RNAi ***,we found that dsRNA can be degraded in midgut fluids,and a dsRNase of ***(AldsRNase)was identified and *** alignment indicated that its 6 key amino acid residues and the Mg2+-binding site were similar to those of other insects’*** signal peptide and endonuclease non-specific domain shared high sequence identity with the brown-winged green stinkbug Plautia stali *** showed high salivary gland and midgut expression and was continuously expressed through the whole life cycle,with peaks at the 4th instar ecdysis in the whole *** purified AldsRNase protein obtained by heterologously expressed can rapidly degrade *** comparing the substrate specificity of AldsRNase,3 specific substrates(dsRNA,small interfering RNA,and dsDNA)were all degraded,and the most efficient degradation is ***,immunofluorescence revealed that AldsRNase was expressed in the cytoplasm of midgut *** cloning and functional study of AldsRNase,the enzyme activity and substrate specificity of the recombinant protein,as well as the subcellular localization of nuclease,the reason for the disappearance of dsRNA was explained,which was useful in improving RNAi efficiency in *** and related species.

关键词： Apolygus lucorum cloning dsRNase enzymatic activity protein expression

Genetic analysis of Chinese families reveals a novel truncation allele of the retinitis pigmentosa GTPase regulator gene

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International Journal of Ophthalmology(English edition) 2014年第5期7卷 753-758页

作者： Fang Hu Xiang-Yun Zeng Lin-Lin Liu Yao-Ling Luo yi-ping jiang Hui Wang Jing Xie Cheng-Quan Hu Lin Gan Liang Huang Department of Ophthalmology the First Affiliated HospitalGannan Medical University Flaum Eye Institute and Department of Ophthalmology School of Medicine and DentistryUniversity of Rochester

AIMTo make comprehensive molecular diagnosis for retinitis pigmentosa (RP) patients in a consanguineous Han Chinese family using next generation sequencing based Capture-NGS screen technology.

关键词： retinitis pigmentosa GTPase regulator retinitis pigmentosa next-generation sequencing genetic diagnosis

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800kV裕隆换流站GIS钢结构的防护

金属材料与冶金工程 2013年第1期41卷 26-29页

作者：易江平湖南省送变电建设公司湖南长沙410131

以裕隆高电压换流站钢结构工程中使用的防护为背景,介绍了冷喷锌涂料的原理、优势及在施工过程中的各项参数。该锌盾镀层能够有效隔绝防腐因子并具有极佳的长效防腐能力。

关键词：钢结构防护冷喷锌镀层

MSP430G2553在智能脉搏测试仪的设计与应用

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自动化技术与应用 2016年第9期35卷 130-133页

作者：易江平仙桃职业学院湖北仙桃433000

本文提出了一种新型的传统脉搏测试仪的数字化设计---利用高性能光电转换传感装置及信号处理电路、MSP430F2553微控制器、液晶显示器等模块组成智能脉搏测试仪,实现了实时显示被测者每分钟的脉搏数目。电路结构简单,所选器件价格合理,... 详细信息

本文提出了一种新型的传统脉搏测试仪的数字化设计---利用高性能光电转换传感装置及信号处理电路、MSP430F2553微控制器、液晶显示器等模块组成智能脉搏测试仪,实现了实时显示被测者每分钟的脉搏数目。电路结构简单,所选器件价格合理,并给出了测试结果 ,达到了设计要求。

关键词：光电传感脉搏测试 MSP430F2553

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精卵融合机制研究进展

生殖与避孕 2008年第8期28卷 482-486页

作者：刘志春江一平福建医科大学细胞生物学与遗传学系细胞与发育工程研究中心

精卵融合是发生在有性生殖过程中最重要的事件之一,其分子机制至今还未完全阐明。基因打靶已经被证明是一个能够揭示细胞间相互作用候选分子功能的权威工具,以前通过抗体抑制试验认为介导了精卵融合的关键性分子因为基因功能的深入研究... 详细信息

精卵融合是发生在有性生殖过程中最重要的事件之一,其分子机制至今还未完全阐明。基因打靶已经被证明是一个能够揭示细胞间相互作用候选分子功能的权威工具,以前通过抗体抑制试验认为介导了精卵融合的关键性分子因为基因功能的深入研究而被一一否定,如整合素、ADAM1、ADAM2、ADAM3。近年来作为参与精卵融合的候选分子,如CD9、Izumo、甘露糖受体等,已经引起广大研究者的关注。

关键词：精卵融合受精卵母细胞精子

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人精子甘露糖受体的表达与精卵融合的关系

生殖与避孕 2005年第11期25卷 671-674页

作者：魏玉珍江一平福建医科大学人体解剖与组织胚胎学系福州350004 福建医科大学细胞与发育工程研究中心福州350004

目的:研究人精子甘露糖受体(MR)的表达与精卵融合的关系。方法:正常人活精子获能培养后与去透明带金黄地鼠卵行精子穿透试验(SPA)监测精卵相互作用,同时用异硫氰酸荧光素偶联的甘露糖化牛血清白蛋白(FITC-DMA)检测精子甘露糖受体的表达... 详细信息

目的:研究人精子甘露糖受体(MR)的表达与精卵融合的关系。方法:正常人活精子获能培养后与去透明带金黄地鼠卵行精子穿透试验(SPA)监测精卵相互作用,同时用异硫氰酸荧光素偶联的甘露糖化牛血清白蛋白(FITC-DMA)检测精子甘露糖受体的表达并分析SPA各项指标与精子甘露糖受体表达的关系。结果:SPA指标中穿透指数、结合指数均与精子MR表达率呈正相关。结论:人精子MR与精-卵膜融合密切相关,可能起某种介导作用。

关键词：甘露糖受体精卵融合精子

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生物油酚醛泡沫制备工艺研究

现代化工 2014年第1期34卷 79-82页

作者：李本伊江平王宇飞常建民北京林业大学材料科学与技术学院北京100083

利用生物油中富含酚类物质的特性,将其与苯酚、多聚甲醛反应合成可发性生物油酚醛树脂,通过调整发泡工艺制备出综合性能优异的生物油酚醛泡沫.结果最佳发泡配方为：以树脂质量分数计,催化剂选用复配比例为m（对甲苯磺酸）/m（磷酸）=2∶... 详细信息

利用生物油中富含酚类物质的特性,将其与苯酚、多聚甲醛反应合成可发性生物油酚醛树脂,通过调整发泡工艺制备出综合性能优异的生物油酚醛泡沫.结果最佳发泡配方为：以树脂质量分数计,催化剂选用复配比例为m（对甲苯磺酸）/m（磷酸）=2∶1的催化剂体系,质量分数为10％～15％;发泡剂选用石油醚,用量为5％～l0％;表面活性剂选用吐温-80,质量分数为5％,发泡温度控制在75℃.得到的生物油酚醛泡沫各项性能良好.

关键词：生物油酚醛树脂发泡工艺

Acute and chronic excitotoxicity in ischemic stroke and late-onset Alzheimer's disease

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Neural Regeneration Research 2025年第7期20卷 1981-1988页

作者： Shan ping Yu Emily Choi Michael Qjiang Ling Wei Department of Anesthesiology Emory University School of MedicineAtlantaGAUSA Center for Visual and Neurocognitive Rehabilitation Atlanta Veterans Affairs Medical CenterDecaturGAUSA

Stroke and Alzheimer's disease are common neurological disorders and often occur in the same *** comorbidity of the two neurological disorders represents a grave health threat to older *** review presents a brief background of the development of novel concepts and their clinical *** activity of glutamatergic N-methyl-D-aspartate receptors and N-methyl-D-aspartate receptor-mediated Ca^(2+)influx is critical for neuronal *** ischemic insult induces prompt and excessive glutamate release and drastic increases of intracellular Ca^(2+)mainly via N-methyl-D-aspartate receptors,particularly of those at the extrasynaptic *** Ca^(2+)-evoked neuronal cell death in the ischemic core is dominated by necrosis within a few hours and days known as acute ***,mild but sustained Ca^(2+)increases under neurodegenerative conditions such as in the distant penumbra of the ischemic brain and early stages of Alzheimer's disease are not immediately toxic,but gradually set off deteriorating Ca^(2+)-dependent signals and neuronal cell loss mostly because of activation of programmed cell death *** on the Ca^(2+)hypothesis of Alzheimer's disease and recent advances,this Ca^(2+)-activated“silent”degenerative excitotoxicity evolves from years to decades and is recognized as a unique slow and chronic *** N-methyl-D-aspartate receptor subunit GluN3A,primarily at the extrasynaptic site,serves as a gatekeeper for the N-methyl-D-aspartate receptor activity and is neuroprotective against both acute and chronic *** stroke and Alzheimer's disease,therefore,share an N-methyl-D-aspartate receptor-and Ca^(2+)-mediated mechanism,although with much different time *** is thus proposed that early interventions to control Ca^(2+)homeostasis at the preclinical stage are pivotal for individuals who are susceptible to sporadic late-onset Alzheimer's disease and Alzheimer's disease-related

关键词： Ca^(2+)hypothesis cognitive deficits hyperactivity late-onset Alzheimer's disease neurodegeneration N-methyl-D-aspartate receptors N-methyl-D-aspartate receptor subunits pathogenesis preventive treatment