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Nanopore sensing system for high-throughput single molecular analysis

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Science China Chemistry 2018年第12期61卷 1483-1485页

作者： Zhen Gu yi-lun ying yi-Tao Long Key Laboratory for Advanced Materials & School of Chemistry and Molecular EngineeringEast China University of Science and Technology Shanghai 200237China

Nanopore technique enables a sensitive, label free, low cost and high throughput method for single-molecule analysis[1–4]. Usually, the nanopore is fabricated on an insulating membrane with electrolyte solution in two sides, which can act as the only pathway for the ionic flow. When a single molecule enters the pore under the applied biased potential, it will temporarily block the ionic current flow through the nanopore. By analyzing the amplitude, duration, shape and frequency spectra of ionic current modulations, nanopore detection could provide the characteristics of the analytes.

关键词： Nanopore sensing system high-throughput single molecular analysis

In-situ discrimination of the water cluster size distribution in aqueous solution by ToF-SIMS

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Science China Chemistry 2018年第2期61卷 159-163页

作者： ying-Ya Liu yi-lun ying Xin Hua yi-Tao Long Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering East China University of Science and Technology

The size distribution and molecular structure of water clusters play a critical role in the chemical,biological and atmospheric *** common experimental study of water clusters in aqueous solution is challenged due to the influence of local H-bonding environments on vibration spectroscopies or vacuum requirements for most mass spectrometry ***,the time-of-flight secondary ion mass spectrometry（To F-SIMS）combining with a microfluidic chip has been applied to achieve the in-situ discrimination of the size distribution for water clusters in liquid water at room *** results demonstrated that the presented method is highly system stable,reproducible and *** comparison of heavy water with pure water was made to further demonstrate the accuracy of this *** results showed that（H2O）3H-＋ and （D2O）4D-＋ are the most dominant clusters in pure and heavy water,*** one water molecule difference in the dominant cluster size may due to the nuclear quantum effects on water’s hydrogen bonded *** is the first time to experimentally show the size distribution of water clusters over a wide range（n=1–30）for pure

关键词： In-situ ToF-SIMS water clusters liquid water microfluidic chip

Nanopore-based sensing interface for single molecule electrochemistry

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Science China Chemistry 2019年第12期62卷 1576-1587页

作者： Rui Gao Yao Lin yi-lun ying yi-Tao Long Department of Chemistry University of UtahSalt Lake CityUtah 84112USA School of Chemistry&Molecular Engineering East China University of Science and TechnologyShanghai 200237China State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

Nanopore techniques are experiencing a gallop since it walked out the notebook and show its charm on the science *** nanoscale pore offers a single-molecule resolution with a label-free and high-selective manner for the research of molecular structures,molecular dynamics,single-molecule reactions and for a variety of applications in biophysics and *** this review,we introduce the construction of three types of nanopore platforms along with the latest progress in DNA sensing,structure and dynamics analysis of peptides/proteins,and the detection of redox reactions with new sensing ***,we depict nanopore data processing methods which provide an insight of data mining under the background ofbig *** could fully expect the great impact of nanopore techniques on not only for DNA sequencing and sensing applications,but also in protein sequencing and clinical diagnostics.

关键词： nanopore single-molecule sensing nanoelectrochemistry

Ultra-low noise measurements of nanopore-based single molecular detection

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Science Bulletin 2017年第18期62卷 1245-1250页

作者： Zhen Gu Huifeng Wang yi-lun ying yi-Tao Long Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering East China University of Science and Technology Shanghai 200237 China School of Information Science and Engineering East China University of Science and Technology Shanghai 200237 China

Nanopore is an ultra-sensitive electrochemical technique for single molecular detection in confined space. To suppress the noise in detection of the weak current of nanopore, we investigated the influence of membrane capacitance and applied voltage on the noise of the current signal by model analysis, simulation and experiment. The obtained results demonstrated that membrane capacitance affects the noise by amplifying the noise of the applied voltage. Therefore, suppression of applied voltage noise is an efficient approach for reducing the noise in nanopore detection. Here, we developed an ultra-low noise instrument system for detecting the single molecule signal in nanopores. As demonstrated by nanopore experiments, the p-p noise of the developed system during the recording is reduced to 3.2B pA using the filter of 5 kHz. Therefore, the developed system could be applied in highly sensitive nanopore detection.

关键词： Nanopore Membrane capacitance Current amplifier Signal amplifier and recording High current resolution

A single biomolecule interface for advancing the sensitivity, selectivity and accuracy of sensors

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National Science Review 2018年第4期5卷 450-452页

作者： yi-lun ying Chan Cao Yong-Xu Hu yi-Tao Long Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering East China University of Science and Technology

The performance of a sensor is mainly determined by its interface with sensing molecules,which comprises reactive molecules for the recognition,active molecules for the signal responses and/or functional materials for signal enhancement(Fig.1a(i)).For example,a double layer is set up at the electrode/electrolyte interface,across which charge-transfer reactions *** a pre-

关键词： A single biomolecule interface for advancing the sensitivity

A Nanopore Phosphorylation Sensor for Single Oligonucleotides and Peptides

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Research 2019年第1期2019卷 415-422页

作者： yi-lun ying Jie Yang Fu-Na Meng Shuang Li Meng-ying Li yi-Tao Long State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Chemistry and Biomedicine Innovation Center Nanjing 210023China School of Chemistry and Molecular Engineering East China University of Science and TechnologyShanghai 200237China

The phosphorylation of oligonucleotides and peptides plays a critical role in regulating virtually all cellular *** fully understand these complex and fundamental regulatory pathways,the cellular phosphorylate changes of both oligonucleotides and peptides should be simultaneously identified and ***,we demonstrated a single-molecule,highthroughput,label-free,general,and one-step aerolysin nanopore method to comprehensively evaluate the phosphorylation of both oligonucleotide and peptide *** virtue of electrochemically confined effects in aerolysin,our results show that the phosphorylation accelerates the traversing speed of a negatively charged substrate for about hundreds of time while significantly enhances the translocation frequency of a positively charged ***,the kinase/phosphatase activity could be directly measured with the aerolysin nanopore from the characteristically dose-dependent event frequency of the *** using this straightforward approach,a model T4 oligonucleotide kinase(PNK)further achieved the nanopore evaluation of its phosphatase activity and real-time monitoring of its phosphatase-catalyzed dephosphorylation at a singlemolecule *** study provides a step forward to nanopore enzymology for analyzing the phosphorylation of both oligonucleotides and peptides with significant feasibility in fundamental biochemical researches,clinical diagnosis,and kinase/phosphatase-targeted drug discovery.

关键词： processes straight catalyzed

An integrated current measurement system for nanopore analysis

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Chinese Science Bulletin 2014年第35期59卷 4968-4973页

作者： Rui Gao yi-lun ying Bing-Yong Yan yi-Tao Long Key Laboratory for Advanced Materials Department of ChemistryEast China University of Science and Technology School of Information Science and Engineering East China University of Science and Technology

Nanopore-based techniques have attracted increasing attention as a unique tool for single-molecule *** accurately detect individual motions of each single molecule,nanopore techniques are used to develop an ultrasensitive current measurement *** work proposes an integrated current measurement system containing an amplifier system and a current signal acquisition system with a high current resolution and a high temporal resolution for nanopore *** exploration and achievements in instrument and signal processing endow nanopore techniques with reliability,affordability,and portability,which make a great leap toward its real applications.

关键词：测量系统纳米孔高电流集成高时间分辨率信号采集系统分子分析信号处理

Exponentially modified Gaussian relevance to the distributions of translocation events in nanopore-based single molecule detection

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Chinese Chemical Letters 2014年第7期25卷 1029-1032页

作者： Zhen Gu yi-lun ying Bing-Yong Yan Hui-Feng Wang Pin-Gang He yi-Tao Long Department of Chemistry East China Normal University Key Laboratory for Advanced Materials & Department of Chemistry East China University of Science and Technology School of Information Science and Engineering East China University of Science and Technology

Nanopore technique plays an important role in single molecule detection, which illuminates the properties of an individual molecule by analyzing the blockage durations and currents. However, the traditional exponential function is lack of efficiency to describe the distributions of blockage durations in nanopore experiments. Herein, we introduced an exponentially modified Gaussian （EMG） function to fit the duration histograms of both simulated events and experimental events. In comparison with the traditional exponential function, our results demonstrated that the EMG provides a better fit while covers the entire range of the distributions. In particular, the fitted parameters of EMG could be directly used to discriminate the sequence length of the oligonucleotides at single molecule level.

关键词： Nanopore Single-molecule detection Exponentially modified Gaussian

An integrated software system for analyzing nanopore data

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Chinese Science Bulletin 2014年第35期59卷 4942-4945页

作者： Na Zhang Yong-Xu Hu Zhen Gu yi-lun ying Pin-Gang He yi-Tao Long Department of Chemistry East China Normal University Key Laboratory for Advanced Materials Department of ChemistryEast China University of Science and Technology

We presented an integrated software system for analyzing nanopore *** self-developed software provided rapid processes for accurate location,classification,and evaluation of every individual *** the proposed software,statistical analysis could be achieved easily and *** results of β-Amyloid 42 demonstrated that our data process could rapidly extract duration time and current *** addition,our data process could accurately carry out statistical fittings.

关键词：软件系统数据集成纳米孔统计分析数据处理淀粉样蛋白分析数据自主研发